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Journal of Advanced Research Jan 2024Mastitis is an inflammatory response in the mammary gland that results in huge economic losses in the breeding industry. The aetiology of mastitis is complex, and the... (Review)
Review
BACKGROUND
Mastitis is an inflammatory response in the mammary gland that results in huge economic losses in the breeding industry. The aetiology of mastitis is complex, and the pathogenesis has not been fully elucidated. It is commonly believed that mastitis is induced by pathogen infection of the mammary gland and induces a local inflammatory response. However, in the clinic, mastitis is often comorbid or secondary to gastric disease, and local control effects targeting the mammary gland are limited. In addition, recent studies have found that the gut/rumen microbiota contributes to the development of mastitis and proposed the gut/rumen-mammary gland axis. Combined with studies indicating that gut/rumen microbiota disturbance can damage the gut mucosa barrier, gut/rumen bacteria and their metabolites can migrate to distal extraintestinal organs. It is believed that the occurrence of mastitis is related not only to the infection of the mammary gland by external pathogenic microorganisms but also to a gastroenterogennic pathogenic pathway.
AIM OF REVIEW
We propose the pathological concept of "gastroenterogennic mastitis" and believe that the gut/rumen-mammary gland axis-mediated pathway is the pathological mechanism of "gastroenterogennic mastitis".
KEY SCIENTIFIC CONCEPTS OF REVIEW
To clarify the concept of "gastroenterogennic mastitis" by summarizing reports on the effect of the gut/rumen microbiota on mastitis and the gut/rumen-mammary gland axis-mediated pathway to provide a research basis and direction for further understanding and solving the pathogenesis and difficulties encountered in the prevention of mastitis.
Topics: Animals; Female; Humans; Rumen; Gastrointestinal Microbiome; Bacteria; Mastitis
PubMed: 36822391
DOI: 10.1016/j.jare.2023.02.009 -
Journal of Mammary Gland Biology and... Jun 2018The One Health concept promotes integrated evaluation of human, animal, and environmental health questions to expedite advances benefiting all species. A recognition of... (Review)
Review
The One Health concept promotes integrated evaluation of human, animal, and environmental health questions to expedite advances benefiting all species. A recognition of the multi-species impact of mastitis as a painful condition with welfare implications leads us to suggest that mastitis is an ideal target for a One Health approach. In this review, we will evaluate the role of the mammary microenvironment in mastitis in humans, ruminants and rabbits, where appropriate also drawing on studies utilising laboratory animal models. We will examine subclinical mastitis, clinical lactational mastitis, and involution-associated, or dry period, mastitis, highlighting important anatomical and immunological species differences. We will synthesise knowledge gained across different species, comparing and contrasting disease presentation. Subclinical mastitis (SCM) is characterised by elevated Na/K ratio, and increased milk IL-8 concentrations. SCM affecting the breastfeeding mother may result in modulation of infant mucosal immune system development, whilst in ruminants notable milk production losses may ensue. In the case of clinical lactational mastitis, we will focus on mastitis caused by Staphylococcus aureus and Escherichia coli. Understanding of the pathogenesis of involution-associated mastitis requires characterization of the structural and molecular changes occurring during involution and we will review these changes across species. We speculate that milk accumulation may act as a nidus for infection, and that the involution 'wound healing phenotype' may render the tissue susceptible to bacterial infection. We will discuss the impact of concurrent pregnancy and a 'parallel pregnancy and involution signature' during bovine mammary involution.
Topics: Animals; Cattle; Cellular Microenvironment; Female; Humans; Mammary Glands, Animal; Mastitis; One Health; Rabbits; Rodentia; Ruminants
PubMed: 29705830
DOI: 10.1007/s10911-018-9395-1 -
Frontiers in Immunology 2022Subclinical mastitis (SCM) is an important risk factor of postnatal HIV-1 transmission that is still poorly understood. A longitudinal sub-study of the ANRS12174 trial... (Clinical Trial)
Clinical Trial
Subclinical mastitis (SCM) is an important risk factor of postnatal HIV-1 transmission that is still poorly understood. A longitudinal sub-study of the ANRS12174 trial including 270 breastfeeding mothers in Lusaka, Zambia measured sodium (Na) and potassium (K) in archived paired breast milk samples collected at week 14, 26 and 38 postpartum to determine cumulative incidence of SCM and the effects of recurrent severe SCM on HIV-1 shedding in breast milk. A nested retrospective cohort study including 112 mothers was also done to determine longitudinal effects of SCM on four pro-inflammatory cytokines; IL6, IL8, IP10 and RANTES. The cumulative incidence for any SCM (Na /K ratio > 0.6) and severe SCM (Na /K ratio > 1) were 58.6% (95%CI: 52.7 - 64.5) and 27.8% (95%CI: 22.5 - 33.1), respectively. In majority of affected mothers (51.4%) severe SCM was recurrent. Both breasts were involved in 11.1%, 33.3% and 70% of the mothers with a single episode, 2 and 3 episodes respectively. In affected breasts, an episode of severe SCM resulted in steep upregulation of the four cytokines considered (IL8, IP10, RANTES and IL6) compared to: before and after the episode; contralateral unaffected breasts; and SCM negative control mothers. Recurrent severe SCM significantly increased the odds of shedding cell-free HIV-1 in breast milk (OR: 5.2; 95%CI: 1.7 - 15.6) whereas single episode of severe SCM did not (OR: 1.8; 95%CI: 0.8 - 4.2). A Na/K ratio > 1 indicative of severe SCM is an excellent indicator of breast inflammation characterized by a steep, localized and temporal upregulation of several pro-inflammatory cytokines that favor HIV-1 shedding in mature breast milk and may facilitate postnatal HIV-1 transmission through breastfeeding.
Topics: Breast Feeding; Chemokine CCL5; Chemokine CXCL10; Cytokines; Female; HIV Infections; HIV-1; Humans; Interleukin-6; Interleukin-8; Mastitis; Retrospective Studies; Sodium; Zambia
PubMed: 35309352
DOI: 10.3389/fimmu.2022.822076 -
Microbiological Research Mar 2022Escherichia coli is one of the leading causes of bovine mastitis; it can cause sub-clinical, and clinical mastitis characterized by systemic changes, abnormal appearance... (Review)
Review
Escherichia coli is one of the leading causes of bovine mastitis; it can cause sub-clinical, and clinical mastitis characterized by systemic changes, abnormal appearance of milk, and udder inflammation. E. coli pathogenicity in the bovine udder is due to the interaction between its virulence factors and the host factors; it was also linked to the presence of a new pathotype termed mammary pathogenic E. coli (MPEC). However, the presence of this pathotype is commonly debated. Its main virulence factor is the lipopolysaccharide (LPS) that is responsible for causing an endotoxic shock, and inducing a strong immune response by binding to the toll-like receptor 4 (TLR4), and stimulating the expression of chemokines (such as IL-8, and RANTES) and pro-inflammatory cytokines (such as IL-6, and IL-1β). This strong immune response could be used to develop alternative and safe approaches to control E. coli causing bovine mastitis by targeting pro-inflammatory cytokines that can damage the host tissue. The need for alternative treatments against E. coli is due to its ability to resist many conventional antibiotics, which is a huge challenge for curing ill animals. Therefore, the aim of this review was to highlight the pathogenicity of E. coli in the mammary gland, discuss the presence of the new putative pathotype, the mammary pathogenic E. coli (MPEC) pathotype, study the host's immune response, and the alternative treatments that are used against mastitis-associated E. coli.
Topics: Animals; Cattle; Complementary Therapies; Escherichia coli; Escherichia coli Infections; Female; Humans; Mastitis; Virulence
PubMed: 35021119
DOI: 10.1016/j.micres.2021.126960 -
International Journal of Environmental... May 2022(1) Background: Breast abscess (BA) is a condition leading in the majority of cases to breastfeeding interruption. Abscesses are commonly treated with antibiotics,... (Observational Study)
Observational Study
(1) Background: Breast abscess (BA) is a condition leading in the majority of cases to breastfeeding interruption. Abscesses are commonly treated with antibiotics, needle aspiration or incision and drainage (I&D), but there is still no consensus on the optimal treatment. Since there are no well-defined clinical guidelines for abscess management, we conducted a retrospective, observational study with the aim of assessing ultrasound (US)-guided management of BA without surgery, regardless of the BA size. The secondary objective was the microbiologic characterization and, in particular, the methicillin resistance identification. (2) Methods: our population included 64 breastfeeding mothers with diagnosis of BA. For every patient, data about maternal, perinatal and breastfeeding features were collected. All patients underwent office US scans and 40 out of 64 required a more detailed breast diagnostic ultrasound performed by a radiologist. In all cases, samples of milk or abscess material were microbiologically tested. All patients received oral antibiotic treatment. We performed needle aspiration, when feasible, even on abscesses greater than 5 cm. (3) Results: most of the women developed BA during the first 100 days (68.8% during the first 60 days) after delivery and 13 needed hospitalization. Four abscesses were bilateral and 16 had a US major diameter greater than 5 cm. All patients were treated with antibiotic therapy according to our clinical protocol and 71.9% (46/64) underwent fine needle aspiration. None of them required I&D. The average duration of breastfeeding was 5 months (IR 2; 9.5) and 40.6% of women with BA continued to breastfeed for more than 6 months. Only 21 mothers interrupted breastfeeding before 3 months. (4) Conclusions: our observational data suggest, regardless of the size and the clinical features of the BA, a conservative approach with antibiotic therapy targeted at the Methicillin-Resistant (MRSA) identified and needle aspiration, if feasible. In our experience, treatment with needle aspiration is a cost- effective method. Unlike drainage, it is an outpatient procedure, easily repeatable, with no cosmetic damage. In addition, it has lower risk of recurrences since, differently from surgical incision, it does not cause interruption of the ducts. Moreover, needle aspiration is less painful, does not require the separation of the mother-child dyad and allows for a quicker, if not immediate, return to breastfeeding.
Topics: Abscess; Anti-Bacterial Agents; Breast Diseases; Breast Feeding; Female; Humans; Mastitis; Pregnancy; Retrospective Studies; Staphylococcus aureus
PubMed: 35565158
DOI: 10.3390/ijerph19095762 -
International Journal of Molecular... Jan 2023Idiopathic granulomatous mastitis (IGM) is a rare and benign inflammatory breast disease with ambiguous aetiology. Contrastingly, lactational mastitis (LM) is commonly...
Idiopathic granulomatous mastitis (IGM) is a rare and benign inflammatory breast disease with ambiguous aetiology. Contrastingly, lactational mastitis (LM) is commonly diagnosed in breastfeeding women. To investigate IGM aetiology, we profiled the microbial flora of pus and skin in patients with IGM and LM. A total of 26 patients with IGM and 6 patients with LM were included in the study. The 16S rRNA sequencing libraries were constructed from 16S rRNA gene amplified from total DNA extracted from pus and skin swabs in patients with IGM and LM controls. Constructed libraries were multiplexed and paired-end sequenced on HiSeq4000. Metagenomic analysis was conducted using modified microbiome abundance analysis suite customised R-resource for paired pus and skin samples. Microbiome multivariable association analyses were performed using linear models. A total of 21 IGM and 3 LM paired pus and skin samples underwent metagenomic analysis. Bray−Curtis ecological dissimilarity distance showed dissimilarity across four sample types (IGM pus, IGM skin, LM pus, and LM skin; PERMANOVA, p < 0.001). No characteristic dominant genus was observed across the IGM samples. The IGM pus samples were more diverse than corresponding IGM skin samples (Shannon and Simpson index; Wilcoxon paired signed-rank tests, p = 0.022 and p = 0.07). Corynebacterium kroppenstedtii, reportedly associated with IGM in the literature, was higher in IGM pus samples than paired skin samples (Wilcoxon, p = 0.022). Three other species and nineteen genera were statistically significant in paired IGM pus−skin comparison after antibiotic treatment adjustment and multiple comparisons correction. Microbial profiles are unique between patients with IGM and LM. Inter-patient variability and polymicrobial IGM pus samples cannot implicate specific genus or species as an infectious cause for IGM.
Topics: Humans; Female; Granulomatous Mastitis; RNA, Ribosomal, 16S; Microbiota; Immunoglobulin M; Suppuration
PubMed: 36674562
DOI: 10.3390/ijms24021042 -
Revue Medicale de Liege Jul 2022The autologous fat injection technique, called lipofilling or lipomodeling, is used in both aesthetic and reconstruction procedures. Lipofilling is rarely accompanied by...
The autologous fat injection technique, called lipofilling or lipomodeling, is used in both aesthetic and reconstruction procedures. Lipofilling is rarely accompanied by complications. We report the case of a young female patient who had undergone breast lipofilling before and who developed significant recurrent but self-limiting inflammatory mastitis in the previously injected breast during two successive pregnancies. To our knowledge, no case of post-lipofilling inflammatory mastitis induced by pregnancy has been described to date. This case suggests an interaction between autologous fat injected into the breast and hormonal impregnation linked to pregnancy. This interaction would create a local environment conducive to the occurrence of an inflammatory reaction, according to a pathophysiological mechanism yet to be defined.
Topics: Adipose Tissue; Breast Neoplasms; Female; Humans; Mammaplasty; Mastitis; Pregnancy
PubMed: 35924505
DOI: No ID Found -
BMC Surgery Apr 2021As a chronic inflammatory disease of an unknown origin, the treatment of granulomatous mastitis has always been controversial. According to some researchers, surgical...
BACKGROUND AND OBJECTIVE
As a chronic inflammatory disease of an unknown origin, the treatment of granulomatous mastitis has always been controversial. According to some researchers, surgical treatment and certain medications, especially steroids, are more effective in treating the disease. This study aimed at evaluating the results of treatment in a group of patients with granulomatous mastitis.
MATERIALS AND METHODS
This longitudinal cohort study evaluated the treatment outcomes of 87 patients with pathology-confirmed granulomatous mastitis referred to the surgical clinic of Central Hospital in Sari, Iran. Demographic, clinical, and pathological information, treatment methods and results, and the recurrence rate were analyzed.
FINDINGS
A total of 87 female patients with granulomatous mastitis aged 22-52 years with a mean age of 34 years were evaluated. All patients had palpable masses; the breast masses were painful in 48.3% of patients, and 55.2% of patients suffered from erythema and inflammation, and8% had fistulas and ulcers at the inflammation site. The patients were followed-up for an average duration of 26 months (8-48 months) after treatment and recovery. The overall recurrence rate was 24.1%, and the recurrence rate was 29.4% in patients underwent surgery, 34.8% in patients received high-dose prednisolone, and 17% in those received low-dose prednisolone together with drainage (p < 0.001).
CONCLUSIONS
According to the results, the low-dose prednisolone plus drainage was more effective with a lower recurrence rate than only surgical excision or high-dose prednisolone. In fact, the use of minimally invasive methods such as drainage plus low-dose steroids is a more effective method with fewer side effects than the other two methods.
Topics: Adult; Breast; Female; Granulomatous Mastitis; Humans; Iran; Longitudinal Studies; Middle Aged; Prednisolone; Young Adult
PubMed: 33882924
DOI: 10.1186/s12893-021-01210-6 -
Animal : An International Journal of... May 2023The present study aimed to investigate the causal relationships between clinical mastitis and some reproductive traits, including success at first insemination (SFI),...
The present study aimed to investigate the causal relationships between clinical mastitis and some reproductive traits, including success at first insemination (SFI), the number of inseminations to pregnancy (INS), the interval from calving to first service (CTFS), first and last service interval (IFL), and open days (OD) in first-parity Holstein cows. For this purpose, the records of 58 281 first parity Holstein cows were analysed. These data sets were collected from 17 large dairy herds from 2008 to 2017. Recursive Mixed Models (RMMs) were applied and compared with the estimations under Standard Mixed Models. Then, one trivariate and three bivariate Gaussian-threshold models were used for the analyses. Recursive models were applied, considering that clinical mastitis can influence fertility traits. Mastitis is considered a covariate for the reproductive traits to determine their causal relationship. The results of this study indicated that causal effects of mastitis on SFI (on the observed scale, %), CTFS, IFL, OD, and INS were -5.7%, 3.3 days, 12.27 days, seven days, and 0.26 services, respectively. The estimated structural coefficients of the recursive models in the first parity imply that mastitis significantly lengthened the fertility interval and decreased the conception rate. In addition, genetic, residual, and phenotypic correlations between mastitis and the reproductive traits under both models were statistically significant. Results of genetic correlations between mastitis and fertility traits suggest that more incidence of mastitis during lactation is related to the delays in the heat show and pregnancy rate after insemination. In summary, considering the causal effects under RMMs may be advantageous to comprehend complicated relationships between complex traits better.
Topics: Pregnancy; Female; Cattle; Animals; Parity; Latent Class Analysis; Reproduction; Fertility; Lactation; Mastitis; Milk; Cattle Diseases
PubMed: 37043934
DOI: 10.1016/j.animal.2023.100777 -
Medical Hypotheses Nov 2020Breast cancer is amongst the most common forms of cancer, is predominantly a woman's illness, and is the most frequently reported invasive cancer in women worldwide...
Breast cancer is amongst the most common forms of cancer, is predominantly a woman's illness, and is the most frequently reported invasive cancer in women worldwide (Bray et al., 2018). Varying risk factors have been identified, including genetics, family history, lifestyle, age and the use of hormone replacement therapy. Mastitis, also predominantly a woman's illness, is an inflammatory condition of the breast that, despite being an inflammation-related condition, is not currently considered a risk factor for breast cancer. This appears counterintuitive as epidemiological studies have identified chronic inflammation as a contributor to cancer risk, for example in gastric, oesophageal and colon cancers (Lin et al., 2016; Qadri et al., 2014; Principe et al., 2017). Previous reports have focused on women hospitalised for mastitis, and most commonly on puerperal mastitis, perhaps underestimating the relationship between breast cancer and non-lactational mastitis. Our hypothesis, based on systematic review, suggests that a longitudinal study of this disease, affecting women predominantly, is warranted.
Topics: Breast Neoplasms; Escherichia coli Infections; Female; Humans; Longitudinal Studies; Mastitis; Risk Factors
PubMed: 32758892
DOI: 10.1016/j.mehy.2020.110057