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Se Pu = Chinese Journal of... Jul 2022This paper provides an annual review of capillary electrophoresis (CE) technology in 2021. A total of 291 research papers related to CE technology published in 2021 were... (Review)
Review
This paper provides an annual review of capillary electrophoresis (CE) technology in 2021. A total of 291 research papers related to CE technology published in 2021 were retrieved from the ISI Web of Science using the keywords, "capillary electrophoresis-mass spectrometry" "capillary isoelectric focusing" "micellar electrokinetic chromatography", or "capillary electrophoresis" (not "capillary electrochromatography" "microchip" and "capillary monolithic column"). In addition, nine research papers related to CE technology in Chinese journals were reviewed: and . This review focused on seven papers published in , , , , and with impact factors (IFs) greater than 10.0, as well as 42 papers reported in , , , and with IFs between 5.0 and 10.0. This review also provides a comprehensive overview of representative CE works in and with IFs<5.0, as well as important Chinese journals, and . According to the IF, this paper introduces the representative work of CE-related papers to allow readers to quickly understand the important research progress of CE technology in the past year.
Topics: Capillary Electrochromatography; Food; Isoelectric Focusing; Micelles; Technology
PubMed: 35791597
DOI: 10.3724/SP.J.1123.2022.03040 -
Proceedings of the Japan Academy.... 2023Micelles are useful and widely applied molecular assemblies, formed from amphiphilic molecules, in water. The majority of amphiphiles possess an alkyl chain as the... (Review)
Review
Micelles are useful and widely applied molecular assemblies, formed from amphiphilic molecules, in water. The majority of amphiphiles possess an alkyl chain as the hydrophobic part. Amphiphiles bearing hydrophilic and hydrophobic polymer chains generate so-called polymeric micelles in water. This review focuses on the recent progress of "aromatic micelles", formed from bent polyaromatic/aromatic amphiphiles, for the development of third-generation micelles. Thanks to multiple host-guest interactions, e.g., the hydrophobic effect and π-π/CH-π interactions, the present micelles display wide-ranging uptake abilities toward various hydrophobic compounds in water. In addition to such host functions, new stimuli-responsive aromatic micelles with pH, light, and redox switches, aromatic oligomer micelles, saccharide-coated aromatic micelles, and related cycloalkane-based micelles were recently developed by our group.
Topics: Micelles; Polymers; Water; Hydrophobic and Hydrophilic Interactions
PubMed: 36631075
DOI: 10.2183/pjab.99.002 -
ACS Biomaterials Science & Engineering Jul 2022Block copolymer micelles have demonstrated great promise in the solubilization of hydrophobic drugs, but an understanding of the blood stability of the drug-laden...
Block copolymer micelles have demonstrated great promise in the solubilization of hydrophobic drugs, but an understanding of the blood stability of the drug-laden micelles is needed for therapeutic advancement of micelle technologies. Following intravenous administration, mPEG-CL and mPEG-LA micelles have demonstrated quick release of their cargo and disassembly in blood, but the prevailing mechanisms of micelle disruption and key biomacromolecules driving this disruption have yet to be elucidated. Although protein interactions with solid polymeric nanoparticles have been characterized, not much is known regarding protein interactions with dynamic block copolymer micelles. Herein, we characterize the interaction of bovine and human serum albumins (BSA and HSA) with polymeric micelles, mPEG-CL and mPEG-LA, using protein fluorescence, isothermal titration calorimetry (ITC), and circular dichroism (CD) spectroscopy. We find that BSA and HSA have interactions with mPEG-CL, while only HSA is observed to weakly interact with mPEG-LA. Protein fluorescence suggests that binding of HSA to mPEG-CL and mPEG-LA is driven by electrostatic interactions. ITC suggests an interaction between serum albumin and mPEG-CL block copolymers driven by hydrogen bonding and electrostatic interactions in physiological MOPS-buffered saline, while mPEG-LA has no measurable interaction with either of the serum albumins. CD spectroscopy demonstrates that the protein secondary structure is intact in both proteins in the presence of mPEG-CL and mPEG-LA. Overall, BSA is not always predictive of polymeric interactions with HSA. Understanding of interactions between serum proteins and block copolymer micelles and the exact mechanisms of destabilization will direct the rational design of block copolymer systems for improving blood stability.
Topics: Animals; Cattle; Humans; Hydrophobic and Hydrophilic Interactions; Micelles; Nanoparticles; Polymers; Serum Albumin
PubMed: 35767337
DOI: 10.1021/acsbiomaterials.2c00016 -
Advanced Healthcare Materials Jul 2022Elastin-like peptides (ELPs) are a versatile platform for tissue engineering and drug delivery. Here, micelle forming ELP chains are genetically fused to three...
Elastin-like peptides (ELPs) are a versatile platform for tissue engineering and drug delivery. Here, micelle forming ELP chains are genetically fused to three therapeutic molecules, keratinocyte growth factor (KGF), stromal cell-derived growth factor 1 (SDF1), and cathelicidin (LL37), to be used in wound healing. Chronic wounds represent a growing problem worldwide. A combinatorial therapy approach targeting different aspects of wound healing would be beneficial, providing a controlled and sustained release of active molecules, while simultaneously protecting these therapeutics from the surrounding harsh wound environment. The results of this study demonstrate that the conjugation of the growth factors KGF and SDF1 and the antimicrobial peptide LL37 to ELPs does not affect the micelle structure and that all three therapeutic moieties retain their bioactivity in vitro. Importantly, when the combination of these micelle ELP nanoparticles are applied to wounds in diabetic mice, over 90 % wound closure is observed, which is significantly higher than when the therapeutics are applied in their naked forms. The application of the nanoparticles designed here is the first report of targeting different aspect of wound healing synergistically.
Topics: Animals; Diabetes Mellitus, Experimental; Elastin; Mice; Micelles; Nanoparticles; Tissue Engineering; Wound Healing
PubMed: 35373501
DOI: 10.1002/adhm.202102795 -
Macromolecular Rapid Communications Aug 2023Fragrances are ubiquitously and extensively used in everyday life and several industrial applications, including perfumes, textiles, laundry formulations, hygiene... (Review)
Review
Fragrances are ubiquitously and extensively used in everyday life and several industrial applications, including perfumes, textiles, laundry formulations, hygiene household products, and food products. However, the intrinsic volatility of these small organic molecules leaves them particularly susceptible to fast depletion from a product or from the surface they have been applied to. Encapsulation is a very effective method to limit the loss of fragrance during their use and to sustain their release. This review gives an overview of the different materials and techniques used for the encapsulation of fragrances, scents, and aromas, as well as the methods used to characterize the resulting encapsulation systems, with a particular focus on cyclodextrins, polymer microcapsules, inorganic microcapsules, block copolymer micelles, and polymersomes for fragrance encapsulation, sustained release, and controlled release.
Topics: Odorants; Micelles; Capsules; Perfume; Polymers
PubMed: 37150605
DOI: 10.1002/marc.202300120 -
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi =... Oct 2022Polymer micelles formed by self-assembly of amphiphilic polymers are widely used in drug delivery, gene delivery and biosensors, due to their special hydrophobic... (Review)
Review
Polymer micelles formed by self-assembly of amphiphilic polymers are widely used in drug delivery, gene delivery and biosensors, due to their special hydrophobic core/hydrophilic shell structure and nanoscale. However, the structural stability of polymer micelles can be affected strongly by environmental factors, such as temperature, pH, shear force in the blood and interaction with non-target cells, leading to degradations and drug leakage as drug carriers. Therefore, researches on the structural integrity and distribution of micelle-based carriers are very important for evaluating their therapeutic effect and clinical feasibility. At present, fluorescence resonance energy transfer (FRET) technology has been widely used in real-time monitoring of aggregation, dissociation and distribution of polymer micelles ( and vo). In this review, the polymer micelles, characteristics of FRET technology, structure and properties of the FRET-polymer micelles are briefly introduced. Then, methods and mechanism for combinations of several commonly used fluorescent probes into polymer micelles structures, and progresses on the stability and distribution studies of FRET-polymer micelles ( and ) as drug carriers are reviewed, and current challenges of FRET technology and future directions are discussed.
Topics: Micelles; Drug Carriers; Polymers; Fluorescence Resonance Energy Transfer; Polyethylene Glycols
PubMed: 36310492
DOI: 10.7507/1001-5515.202111040 -
Biophysical Journal Aug 2020The functional roles of the lipid asymmetry of biomembranes are attracting increasing attention. This study characterizes the activity of surfactants to induce...
The functional roles of the lipid asymmetry of biomembranes are attracting increasing attention. This study characterizes the activity of surfactants to induce transmembrane flip-flop of lipids and thus "scramble" this asymmetry. Detergent-induced lipid scrambling of liposomes mimicking the charge asymmetry of bacterial membranes with 20 mol % of 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-rac-glycerol in the outer leaflet only was quantified by ζ-potential measurements for octaethylene glycol dodecyl ether (CEO), octyl glucoside (OG), and dodecyl maltoside. Membrane leakage was separately measured by the fluorescence lifetime-based calcein leakage assay and the onset of the membrane-to-micelle transition by isothermal titration calorimetry. Partition coefficients and partial molar areas were obtained as well. For the quickly membrane-permeant CEO and OG, leakage proceeds at a rather sharp threshold content in the membrane, which is well below the onset of solubilization and little dependent on incubation time; it is accompanied by fast lipid scrambling. However, unlike leakage, flip-flop is a relaxation process that speeds up gradually from taking weeks in the detergent-free membrane to minutes or less in the leaking membrane. Hence, after 24 h of incubation, 10 mol % of CEO or 50 mol % of OG in the membrane suffice for virtually complete lipid scrambling, whereas leakage remains below 10% for up to 14 mol % of CEO and 88 mol % of OG. There is thus a concentration window in which lipid scrambling proceeds without leakage. This implies that lipid scrambling must be considered a possible mode of action of antimicrobial peptides and other membrane-active drugs or biomolecules. A related, detergent-based protocol for scrambling the lipid asymmetry of liposomes and maybe cells without compromising their overall integrity would be a very valuable tool to study functions of lipid asymmetry.
Topics: Calorimetry; Lipid Bilayers; Lipids; Liposomes; Micelles; Phosphatidylcholines
PubMed: 32738218
DOI: 10.1016/j.bpj.2020.07.004 -
Proceedings of the National Academy of... Mar 2024Carriers for RNA delivery must be dynamic, first stabilizing and protecting therapeutic RNA during delivery to the target tissue and across cellular membrane barriers...
Carriers for RNA delivery must be dynamic, first stabilizing and protecting therapeutic RNA during delivery to the target tissue and across cellular membrane barriers and then releasing the cargo in bioactive form. The chemical space of carriers ranges from small cationic lipids applied in lipoplexes and lipid nanoparticles, over medium-sized sequence-defined xenopeptides, to macromolecular polycations applied in polyplexes and polymer micelles. This perspective highlights the discovery of distinct virus-inspired dynamic processes that capitalize on mutual nanoparticle-host interactions to achieve potent RNA delivery. From the host side, subtle alterations of pH, ion concentration, redox potential, presence of specific proteins, receptors, or enzymes are cues, which must be recognized by the RNA nanocarrier via dynamic chemical designs including cleavable bonds, alterable physicochemical properties, and supramolecular assembly-disassembly processes to respond to changing biological microenvironment during delivery.
Topics: Cell Membrane; Cues; Micelles; Polymers; RNA
PubMed: 38437544
DOI: 10.1073/pnas.2307799120 -
Science Advances Aug 2022Altered host-microbe interactions and increased intestinal permeability have been implicated in disease pathogenesis. However, the mechanisms by which intestinal...
Altered host-microbe interactions and increased intestinal permeability have been implicated in disease pathogenesis. However, the mechanisms by which intestinal microbes affect epithelial barrier integrity remain unclear. Here, we investigate the impact of bacterial metabolism of host-produced bile acid (BA) metabolites on epithelial barrier integrity. We observe that rats fed a choline-deficient, l-amino acid-defined, high-fat diet (CDAHFD) exhibit reduced intestinal abundance of host-produced conjugated BAs at early time points, coinciding with increased gut permeability. We show that in vitro, conjugated BAs protect gut epithelial monolayers from damage caused by bacterially produced unconjugated BAs through micelle formation. We then demonstrate that inhibition of bacterial BA deconjugation with a small-molecule inhibitor prevents the development of pathologic intestinal permeability and hepatic inflammation in CDAHFD-fed rats. Our study identifies a signaling-independent, physicochemical mechanism for conjugated BA-mediated protection of epithelial barrier function and suggests that rational manipulation of microbial BA metabolism could be leveraged to regulate gut barrier integrity.
Topics: Animals; Bile Acids and Salts; Gastrointestinal Microbiome; Liver; Micelles; Permeability; Rats
PubMed: 36026454
DOI: 10.1126/sciadv.abo2794 -
International Journal of Molecular... Jun 2021Lipophilicity of 15 derivatives of sodium cholate, defined by the octan-1-ol/water partition coefficient (), has been theoretically determined by the method....
Lipophilicity of 15 derivatives of sodium cholate, defined by the octan-1-ol/water partition coefficient (), has been theoretically determined by the method. These derivatives bear highly hydrophobic or highly hydrophilic substituents at the C3 position of the steroid nucleus, being linked to it through an amide bond. The difference between the maximum value of and the minimum one is enlarged to 3.5. The partition coefficient and the critical micelle concentration () are tightly related by a double-logarithm relationship (VirtuallogP=-(1.00±0.09)log(cmcmM)+(2.79±0.09)), meaning that the Gibbs free energies for the transfer of a bile anion from water to either a micelle or to octan-1-ol differ by a constant. The equation also means that can be used as a measurement of lipophilicity. The demicellization of the aggregates formed by three derivatives of sodium cholate bearing bulky hydrophobic substituents has been studied by surface tension and isothermal titration calorimetry. Aggregation numbers, enthalpies, free energies, entropies, and heat capacities, Δ, were obtained. Δ, being positive, means that the interior of the aggregates is hydrophobic.
Topics: Algorithms; Bile Acids and Salts; Calorimetry; Chemical Phenomena; Hydrophobic and Hydrophilic Interactions; Micelles; Models, Theoretical; Molecular Structure; Thermodynamics
PubMed: 34206572
DOI: 10.3390/ijms22136684