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International Journal of Molecular... Feb 2023The aim of this Special Issue is to highlight the molecular dialogue between the pollen tube and the pistil [...].
The aim of this Special Issue is to highlight the molecular dialogue between the pollen tube and the pistil [...].
Topics: Pollen Tube; Cell Communication; Pollination; Flowers
PubMed: 36835118
DOI: 10.3390/ijms24043707 -
Science (New York, N.Y.) Dec 2017In flowering plants, fertilization requires complex cell-to-cell communication events between the pollen tube and the female reproductive tissues, which are controlled...
In flowering plants, fertilization requires complex cell-to-cell communication events between the pollen tube and the female reproductive tissues, which are controlled by extracellular signaling molecules interacting with receptors at the pollen tube surface. We found that two such receptors in , BUPS1 and BUPS2, and their peptide ligands, RALF4 and RALF19, are pollen tube-expressed and are required to maintain pollen tube integrity. BUPS1 and BUPS2 interact with receptors ANXUR1 and ANXUR2 via their ectodomains, and both sets of receptors bind RALF4 and RALF19. These receptor-ligand interactions are in competition with the female-derived ligand RALF34, which induces pollen tube bursting at nanomolar concentrations. We propose that RALF34 replaces RALF4 and RALF19 at the interface of pollen tube-female gametophyte contact, thereby deregulating BUPS-ANXUR signaling and in turn leading to pollen tube rupture and sperm release.
Topics: Arabidopsis; Arabidopsis Proteins; Fertilization; Ligands; Pollen Tube; Protein Kinases; Signal Transduction
PubMed: 29242234
DOI: 10.1126/science.aao3642 -
Cell Oct 2023Pollen-pistil interactions establish interspecific/intergeneric pre-zygotic hybridization barriers in plants. The rejection of undesired pollen at the stigma is crucial...
Pollen-pistil interactions establish interspecific/intergeneric pre-zygotic hybridization barriers in plants. The rejection of undesired pollen at the stigma is crucial to avoid outcrossing but can be overcome with the support of mentor pollen. The mechanisms underlying this hybridization barrier are largely unknown. Here, in Arabidopsis, we demonstrate that receptor-like kinases FERONIA/CURVY1/ANJEA/HERCULES RECEPTOR KINASE 1 and cell wall proteins LRX3/4/5 interact on papilla cell surfaces with autocrine stigmatic RALF1/22/23/33 peptide ligands (sRALFs) to establish a lock that blocks the penetration of undesired pollen tubes. Compatible pollen-derived RALF10/11/12/13/25/26/30 peptides (pRALFs) act as a key, outcompeting sRALFs and enabling pollen tube penetration. By treating Arabidopsis stigmas with synthetic pRALFs, we unlock the barrier, facilitating pollen tube penetration from distantly related Brassicaceae species and resulting in interspecific/intergeneric hybrid embryo formation. Therefore, we uncover a "lock-and-key" system governing the hybridization breadth of interspecific/intergeneric crosses in Brassicaceae. Manipulating this system holds promise for facilitating broad hybridization in crops.
Topics: Arabidopsis; Arabidopsis Proteins; Brassicaceae; Peptide Hormones; Peptides; Pollen; Pollen Tube; Reproductive Isolation
PubMed: 37806310
DOI: 10.1016/j.cell.2023.09.003 -
Science (New York, N.Y.) Jan 2022Fertilization of an egg by multiple sperm (polyspermy) leads to lethal genome imbalance and chromosome segregation defects. In , the block to polyspermy is facilitated...
Fertilization of an egg by multiple sperm (polyspermy) leads to lethal genome imbalance and chromosome segregation defects. In , the block to polyspermy is facilitated by a mechanism that prevents polytubey (the arrival of multiple pollen tubes to one ovule). We show here that FERONIA, ANJEA, and HERCULES RECEPTOR KINASE 1 receptor-like kinases located at the septum interact with pollen tube-specific RALF6, 7, 16, 36, and 37 peptide ligands to establish this polytubey block. The same combination of RALF (rapid alkalinization factor) peptides and receptor complexes controls pollen tube reception and rupture inside the targeted ovule. Pollen tube rupture releases the polytubey block at the septum, which allows the emergence of secondary pollen tubes upon fertilization failure. Thus, orchestrated steps in the fertilization process in are coordinated by the same signaling components to guarantee and optimize reproductive success.
Topics: Arabidopsis; Arabidopsis Proteins; Fertilization; Ligands; Ovule; Peptides; Phosphotransferases; Pollen; Pollen Tube; Pollination; Protein Kinases; Signal Transduction
PubMed: 35050671
DOI: 10.1126/science.abl4683 -
The New Phytologist Oct 2018
Topics: Cell Wall; Mechanical Phenomena; Plant Proteins; Pollen Tube; Pollination
PubMed: 30156021
DOI: 10.1111/nph.15358 -
International Journal of Molecular... Nov 2021Angiosperm mature pollen represents a quiescent stage with a desiccated cytoplasm surrounded by a tough cell wall, which is resistant to the suboptimal environmental... (Review)
Review
Angiosperm mature pollen represents a quiescent stage with a desiccated cytoplasm surrounded by a tough cell wall, which is resistant to the suboptimal environmental conditions and carries the genetic information in an intact stage to the female gametophyte. Post pollination, pollen grains are rehydrated, activated, and a rapid pollen tube growth starts, which is accompanied by a notable metabolic activity, synthesis of novel proteins, and a mutual communication with female reproductive tissues. Several angiosperm species (, tobacco, maize, and kiwifruit) were subjected to phosphoproteomic studies of their male gametophyte developmental stages, mostly mature pollen grains. The aim of this review is to compare the available phosphoproteomic studies and to highlight the common phosphoproteins and regulatory trends in the studied species. Moreover, the pollen phosphoproteome was compared with root hair phosphoproteome to pinpoint the common proteins taking part in their tip growth, which share the same cellular mechanisms.
Topics: Phosphoproteins; Plant Proteins; Pollen Tube; Pollination; Proteome; Proteomics
PubMed: 34830092
DOI: 10.3390/ijms222212212 -
Frontiers in Plant Science 2022Double fertilization is a unique and particularly complicated process for the generation alternation of angiosperms. Sperm cells of angiosperms lose the motility... (Review)
Review
Double fertilization is a unique and particularly complicated process for the generation alternation of angiosperms. Sperm cells of angiosperms lose the motility compared with that of gymnosperms. The sperm cells are passively carried and transported by the pollen tube for a long journey before targeting the ovule. Two sperm cells are released at the cleft between the egg and the central cell and fused with two female gametes to produce a zygote and endosperm, respectively, to accomplish the so-called double fertilization process. In this process, extensive communication and interaction occur between the male (pollen or pollen tube) and the female (ovule). It is suggested that small peptides and receptor kinases play critical roles in orchestrating this cell-cell communication. Here, we illuminate the understanding of phases in the process, such as pollen-stigma recognition, the hydration and germination of pollen grains, the growth, guidance, and rupture of tubes, the release of sperm cells, and the fusion of gametes, by reviewing increasing data recently. The roles of peptides and receptor kinases in signaling mechanisms underlying cell-cell communication were focused on, and directions of future studies were perspected in this review.
PubMed: 36589119
DOI: 10.3389/fpls.2022.1090836 -
FEBS Letters Sep 2022The delivery of sperm cells via tip-growing pollen tubes is an innovation of seed plants and shows the importance of pollen tubes for reproduction and their specific... (Review)
Review
The delivery of sperm cells via tip-growing pollen tubes is an innovation of seed plants and shows the importance of pollen tubes for reproduction and their specific growth kinetics. Fast-growing pollen tubes demand an extensive and dynamic vesicular trafficking network to build new cell membrane and wall, to deliver proteins among endomembrane compartments, and also to respond to external stimuli for growth adjustment. In this review, we summarize current findings on endomembrane compartments and vesicular trafficking routes of pollen tubes, comparing and contrasting their features with those of most somatic cells. We discuss the importance of membrane homeostasis, either at the plasma membrane (PM) or between PM-targeted trafficking and vacuolar trafficking, for pollen tube growth. We also provide perspectives to facilitate future studies of vesicular trafficking in pollen tubes.
Topics: Arabidopsis; Arabidopsis Proteins; Pollen Tube; Seeds; Vacuoles
PubMed: 35348201
DOI: 10.1002/1873-3468.14343 -
International Journal of Molecular... Jan 2019The pollen tube was a fundamental step forward in the evolution of terrestrial plants; in fact, it allowed plants to liberate themselves from water demand during...
The pollen tube was a fundamental step forward in the evolution of terrestrial plants; in fact, it allowed plants to liberate themselves from water demand during reproduction [...].
Topics: Plant Physiological Phenomena; Pollen Tube; Reproduction
PubMed: 30691221
DOI: 10.3390/ijms20030531 -
Plants (Basel, Switzerland) Jun 2021Pollen germination and pollen tube growth are common to all seed plants, but these processes first developed in gymnosperms and still serve for their successful sexual... (Review)
Review
Pollen germination and pollen tube growth are common to all seed plants, but these processes first developed in gymnosperms and still serve for their successful sexual reproduction. The main body of data on the reproductive physiology, however, was obtained on flowering plants, and one should be careful to extrapolate the discovered patterns to gymnosperms. In recent years, physiological studies of coniferous pollen have been increasing, and both the features of this group and the similarities with flowering plants have already been identified. The main part of the review is devoted to physiological studies carried out on conifer pollen. The main properties and diversity of pollen grains and pollination strategies in gymnosperms are described.
PubMed: 34206892
DOI: 10.3390/plants10071301