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The Journal of Experimental Biology Mar 2023Fungicides and herbicides are two of the most heavily applied pesticide classes in the world, but receive little research attention with regards to their potential...
Fungicides and herbicides are two of the most heavily applied pesticide classes in the world, but receive little research attention with regards to their potential impacts on bees. As they are not designed to target insects, the mechanisms behind potential impacts of these pesticides are unclear. It is therefore important to understand their influence at a range of levels, including sublethal impacts on behaviours such as learning. We used the proboscis extension reflex (PER) paradigm to assess how the herbicide glyphosate and the fungicide prothioconazole affect bumblebee olfactory learning. We also assessed responsiveness, and compared the impacts of these active ingredients and their respective commercial formulations (Roundup Biactive and Proline). We found that learning was not impaired by either formulation but, of the bees that displayed evidence of learning, exposure to prothioconazole active ingredient increased learning level in some situations, while exposure to glyphosate active ingredient resulted in bumblebees being less likely to respond to antennal stimulation with sucrose. Our data suggest that fungicides and herbicides may not negatively impact olfactory learning ability when bumblebees are exposed orally to field-realistic doses in a lab setting, but that glyphosate has the potential to cause changes in responsiveness in bees. As we found impacts of active ingredients and not commercial formulations, this suggests that co-formulants may modify impacts of active ingredients in the products tested on olfactory learning without being toxic themselves. More research is needed to understand the mechanisms behind potential impacts of fungicides and herbicides on bees, and to evaluate the implications of behavioural changes caused by glyphosate and prothioconazole for bumblebee fitness.
Topics: Bees; Animals; Herbicides; Fungicides, Industrial; Learning; Conditioning, Classical; Smell
PubMed: 36861783
DOI: 10.1242/jeb.245180 -
Parasites & Vectors Dec 2018Functional inference on the attachment of acanthocephalans has generally been drawn directly from morphology. However, performance of structures is often non-intuitive...
BACKGROUND
Functional inference on the attachment of acanthocephalans has generally been drawn directly from morphology. However, performance of structures is often non-intuitive and context-dependent, thus performance analysis should be included whenever possible to improve functional interpretation. In acanthocephalans, performance analysis of attachment is available only for Acanthocephalus ranae, a species that solely relies on the proboscis to attach. Here we compare body morphology and muscle arrangement in 13 species of Corynosoma, which use their spiny body as a fundamental holdfast. A basic performance analysis using live cystacanths of two representative species is also provided.
METHODS
Adults of 13 Corynosoma spp. were obtained from 11 marine mammal species. Specimens were cut and carefully cleaned to examine muscle arrangement through light and scanning electron microscopy. Live cystacanths of C. australe and C. cetaceum were selected for performance analysis. Video records of evagination-invagination cycles of the proboscis were obtained and analysed with a video editor.
RESULTS
The basic arrangement of proboscis retractors, trunk circular and longitudinal muscles, neck retractors and receptacle retractors, was conserved in all Corynosoma species. Interspecific variability was found in the relative development of disk muscles: minimum in C. enhydri, maximum in C. cetaceum; the distal insertion of the ventral neck retractor: ventro-lateral in C. cetaceum, C. hamannni and C. pseudohamanni and ventral in the other species; and the distal insertion of the receptacle retractors: more proximal in species with a longer hindtrunk. Performance analysis indicated striking similarities to that described for A. ranae except that (i) the foretrunk bends ventrally during the evagination-invagination cycles of the proboscis; (ii) disk muscles can flatten the tip of the foretrunk regardless of these cycles; and (iii) the receptacle bends ventrally and is driven to the hindtrunk by coordinated action of receptacle retractors.
CONCLUSIONS
Species of Corynosoma are able to use up to six holfast mechanisms. Attachment relies on a similar performance to that described for A. ranae. However, structural ventral bending of an inflated, spiny foretrunk, with a parallel re-arrangement of foretrunk muscles, have generated unexpected novel functions that make attachment extremely effective in species of Corynosoma. Interspecific variability in trunk shape and muscle arrangement grossly correlates with the rheological conditions each species experiences in their microhabitats within the gut of marine mammals.
Topics: Acanthocephala; Animals; Female; Helminthiasis, Animal; Host-Parasite Interactions; Male; Mammals; Microscopy, Electron, Scanning; Muscles; Seawater; Video Recording
PubMed: 30545426
DOI: 10.1186/s13071-018-3165-1 -
Current Biology : CB Nov 2020Hemichordate relationships remain contentious due to conflicting molecular results [1-7] and the high degree of morphological disparity between the two hemichordate...
Hemichordate relationships remain contentious due to conflicting molecular results [1-7] and the high degree of morphological disparity between the two hemichordate classes, Enteropneusta and Pterobranchia [8-11]. Additionally, hemichordates have a poor fossil record outside of the Cambrian, with the exception of the collagenous tubes of the pterobranchs (which include graptolites). By the middle Cambrian, tube-dwelling colonial pterobranchs [12, 13] and tube-dwelling enteropneusts coexisted [14, 15], supporting the origin of the hemichordate body plan earlier in the Cambrian without clarifying the morphology of their last common ancestor. Here, we describe a new hemichordate, Gyaltsenglossus senis, based on 33 specimens from the 506-million-year-old Burgess Shale (Odaray Mountain, British Columbia). G. senis has a unique combination of soft anatomical characters found in both extant classes of hemichordates, namely a trimeric-vermiform body plan with an elongate proboscis and six feeding arms with tentacles. The trunk possesses a long through-gut and terminates with a bulbous structure potentially used for locomotion and/or as a temporary anchor. There is no evidence of a secreted tube. Our phylogenetic analyses retrieve this new taxon as a stem-group hemichordate, supporting the hypothesis that a vermiform body plan preceded both tube building and colonial ecologies. This new taxon suggests that a bimodal feeding ecology using tentacles to filter feed and a proboscis to deposit feed may be plesiomorphic in hemichordates. Finally, the presence of a muscular, post-anal attachment structure in all known Cambrian hemichordates supports this feature as an additional hemichordate plesiomorphy critical for understanding early hemichordate evolution.
Topics: Animals; Biological Evolution; Chordata, Nonvertebrate; Feeding Behavior; Fossils; Locomotion; Phylogeny
PubMed: 32857969
DOI: 10.1016/j.cub.2020.07.078 -
Current Biology : CB Nov 2021The elephant proboscis (trunk), which functions as a muscular hydrostat with a virtually infinite number of degrees of freedom, is a spectacular organ for delicate to...
The elephant proboscis (trunk), which functions as a muscular hydrostat with a virtually infinite number of degrees of freedom, is a spectacular organ for delicate to heavy object manipulation as well as social and sensory functions. Using high-resolution motion capture and functional morphology analyses, we show here that elephants evolved strategies that reduce the biomechanical complexity of their trunk. Indeed, our behavioral experiments with objects of various shapes, sizes, and weights indicate that (1) complex behaviors emerge from the combination of a finite set of basic movements; (2) curvature, torsion, and strain provide an appropriate kinematic representation, allowing us to extract motion primitives from the trunk trajectories; (3) transport of objects involves the proximal propagation of an inward curvature front initiated at the tip; (4) the trunk can also form pseudo-joints for point-to-point motion; and (5) the trunk tip velocity obeys a power law with its path curvature, similar to human hand drawing movements. We also reveal with unprecedented precision the functional anatomy of the African and Asian elephant trunks using medical imaging and macro-scale serial sectioning, thus drawing strong connections between motion primitives and muscular synergies. Our study is the first combined quantitative analysis of the mechanical performance, kinematic strategies, and functional morphology of the largest animal muscular hydrostat on Earth. It provides data for developing innovative "soft-robotic" manipulators devoid of articulations, replicating the high compliance, flexibility, and strength of the elephant trunk. VIDEO ABSTRACT.
Topics: Animals; Biomechanical Phenomena; Movement; Nose; Robotics; Torso
PubMed: 34428468
DOI: 10.1016/j.cub.2021.08.029 -
Frontiers in Physiology 2018Niemann-Pick proteins type C2 (NPC2) are carriers of cholesterol in vertebrates, with a single member in each species. The high sequence conservation between mammals and...
Niemann-Pick proteins type C2 (NPC2) are carriers of cholesterol in vertebrates, with a single member in each species. The high sequence conservation between mammals and across vertebrates is related to their common function. In contrast, NPC2 proteins in arthropods have undergone extensive duplication and differentiation, probably under environmental pressure, and are likely to have different functions. Recent studies have suggested that in arthropods these proteins might act as carriers for semiochemicals and other hydrophobic compounds. In this study we focused on the function of a specific NPC2 gene in the moth (HarmNPC2-1). This protein binds several flavonoids with micromolar dissociation constants. The best ligand was gossypol, present in cotton, one of the main host plants for . Western blot revealed the presence of HarmNPC2-1 in different parts of the body, including the antennae, proboscis, and abdomen. In the antennae, hybridization experiments produced strong staining in auxiliary cells at the base of sensilla trichodea, basiconica, coeloconica, and chaetica. Immunocytochemistry confirmed the expression of the protein in sensilla chaetica. Our results support a role of semiochemical carriers for NPC2 proteins in insects and indicate such proteins as new targets for insecticide-free pest population control.
PubMed: 29472868
DOI: 10.3389/fphys.2018.00052 -
Journal of Visualized Experiments : JoVE Apr 2018Heteropteran insects such as assassin bugs (Reduviidae) and giant water bugs (Belostomatidae) descended from a common predaceous and venomous ancestor, and the majority...
Heteropteran insects such as assassin bugs (Reduviidae) and giant water bugs (Belostomatidae) descended from a common predaceous and venomous ancestor, and the majority of extant heteropterans retain this trophic strategy. Some heteropterans have transitioned to feeding on vertebrate blood (such as the kissing bugs, Triatominae; and bed bugs, Cimicidae) while others have reverted to feeding on plants (most Pentatomomorpha). However, with the exception of saliva used by kissing bugs to facilitate blood-feeding, little is known about heteropteran venoms compared to the venoms of spiders, scorpions and snakes. One obstacle to the characterization of heteropteran venom toxins is the structure and function of the venom/labial glands, which are both morphologically complex and perform multiple biological roles (defense, prey capture, and extra-oral digestion). In this article, we describe three methods we have successfully used to collect heteropteran venoms. First, we present electrostimulation as a convenient way to collect venom that is often lethal when injected into prey animals, and which obviates contamination by glandular tissue. Second, we show that gentle harassment of animals is sufficient to produce venom extrusion from the proboscis and/or venom spitting in some groups of heteropterans. Third, we describe methods to harvest venom toxins by dissection of anaesthetized animals to obtain the venom glands. This method is complementary to other methods, as it may allow harvesting of toxins from taxa in which electrostimulation and harassment are ineffective. These protocols will enable researchers to harvest toxins from heteropteran insects for structure-function characterization and possible applications in medicine and agriculture.
Topics: Animals; Insecta; Reduviidae; Toxins, Biological; Venoms
PubMed: 29733320
DOI: 10.3791/57729 -
The Journal of Experimental Biology May 2022Multisensory integration is assumed to entail benefits for receivers across multiple ecological contexts. However, signal integration effectiveness is constrained by...
Multisensory integration is assumed to entail benefits for receivers across multiple ecological contexts. However, signal integration effectiveness is constrained by features of the spatiotemporal and intensity domains. How sensory modalities are integrated during tasks facilitated by learning and memory, such as pollination, remains unsolved. Honey bees use olfactory and visual cues during foraging, making them a good model to study the use of multimodal signals. Here, we examined the effect of stimulus intensity on both learning and memory performance of bees trained using unimodal or bimodal stimuli. We measured the performance and the latency response across planned discrete levels of stimulus intensity. We employed the conditioning of the proboscis extension response protocol in honey bees using an electromechanical setup allowing us to control simultaneously and precisely olfactory and visual stimuli at different intensities. Our results show that the bimodal enhancement during learning and memory was higher as the intensity decreased when the separate individual components were least effective. Still, this effect was not detectable for the latency of response. Remarkably, these results support the principle of inverse effectiveness, traditionally studied in vertebrates, predicting that multisensory stimuli are more effectively integrated when the best unisensory response is relatively weak. Thus, we argue that the performance of the bees while using a bimodal stimulus depends on the interaction and intensity of its individual components. We further hold that the inclusion of findings across all levels of analysis enriches the traditional understanding of the mechanics and reliance of complex signals in honey bees.
Topics: Animals; Bees; Cues; Learning; Smell
PubMed: 35531628
DOI: 10.1242/jeb.243832 -
Molecules (Basel, Switzerland) Jun 2021The past decade has seen growing interest in marine natural pigments for biotechnological applications. One of the most abundant classes of biological pigments is the... (Comparative Study)
Comparative Study
The past decade has seen growing interest in marine natural pigments for biotechnological applications. One of the most abundant classes of biological pigments is the tetrapyrroles, which are prized targets due their photodynamic properties; porphyrins are the best known examples of this group. Many animal porphyrinoids and other tetrapyrroles are produced through heme metabolic pathways, the best known of which are the bile pigments biliverdin and bilirubin. is a marine Polychaeta characterized by its bright green coloration resulting from a remarkably wide range of greenish and yellowish tetrapyrroles, some of which have promising photodynamic properties. The present study combined metabolomics based on HPLC-DAD with RNA-seq transcriptomics to investigate the molecular pathways of porphyrinoid metabolism by comparing the worm's proboscis and epidermis, which display distinct pigmentation patterns. The results showed that pigments are endogenous and seemingly heme-derived. The worm possesses homologs in both organs for genes encoding enzymes involved in heme metabolism such as ALAD, FECH, UROS, and PPOX. However, the findings also indicate that variants of the canonical enzymes of the heme biosynthesis pathway can be species- and organ-specific. These differences between molecular networks contribute to explain not only the differential pigmentation patterns between organs, but also the worm's variety of novel endogenous tetrapyrrolic compounds.
Topics: Animals; Chromatography, High Pressure Liquid; Epidermis; Gene Expression Profiling; Gene Regulatory Networks; Metabolic Networks and Pathways; Metabolomics; Organ Specificity; Photosensitizing Agents; Polychaeta; Sequence Analysis, RNA; Species Specificity; Tetrapyrroles
PubMed: 34198975
DOI: 10.3390/molecules26133924 -
BMC Evolutionary Biology Jan 2017Arthropoda, Tardigrada and Onychophora evolved from lobopodians, a paraphyletic group of disparate Palaeozoic vermiform animals with soft legs. Although the...
BACKGROUND
Arthropoda, Tardigrada and Onychophora evolved from lobopodians, a paraphyletic group of disparate Palaeozoic vermiform animals with soft legs. Although the morphological diversity that this group encompasses likely illustrates the importance of niche diversification in the early radiation of panarthropods, the ecology of lobopodians remains poorly characterized.
RESULTS
Here we describe a new luolishaniid taxon from the middle Cambrian Burgess Shale (Walcott Quarry) in British Columbia, Canada, whose specialized morphology epitomizes the suspension-feeding ecology of this clade, and is convergent with some modern marine animals, such as caprellid crustaceans. This species possesses two long pairs and four shorter pairs of elongate spinose lobopods at the front, each bearing two slender claws, and three pairs of stout lobopods bearing single, strong, hook-like anterior-facing claws at the back. The trunk is remarkably bare, widening rearwards, and, at the front, extends beyond the first pair of lobopods into a small "head" bearing a pair of visual organs and a short proboscis with numerous teeth. Based on a critical reappraisal of character coding in lobopodians and using Bayesian and parsimony-based tree searches, two alternative scenarios for early panarthropod evolution are retrieved. In both cases, hallucigeniids and luolishaniids are found to be extinct radiative stem group panarthropods, in contrast to previous analyses supporting a position of hallucigeniids as part of total-group Onychophora. Our Bayesian topology finds luolishaniids and hallucigeniids to form two successive clades at the base of Panarthropoda. Disparity analyses suggest that luolishaniids, hallucigeniids and total-group Onychophora each occupy a distinct region of morphospace.
CONCLUSIONS
Hallucigeniids and luolishaniids were comparably diverse and successful, representing two major lobopodian clades in the early Palaeozoic, and both evolved body plans adapted to different forms of suspension feeding. A Bayesian approach to cladistics supports the view that a semi-sessile, suspension-feeding lifestyle characterized the origin and rise of Panarthropoda from cycloneuralian body plans.
Topics: Animals; Arthropods; Bayes Theorem; Biological Evolution; Fossils; Phylogeny
PubMed: 28137244
DOI: 10.1186/s12862-016-0858-y -
Nature Communications Nov 2022A crucial step in the evolution of Euarthropoda (chelicerates, myriapods, pancrustaceans) was the transition between fossil groups that possessed frontal appendages...
A crucial step in the evolution of Euarthropoda (chelicerates, myriapods, pancrustaceans) was the transition between fossil groups that possessed frontal appendages innervated by the first segment of the brain (protocerebrum), and living groups with a protocerebral labrum and paired appendages innervated by the second brain segment (deutocerebrum). Appendage homologies between the groups are controversial. Here we describe two specimens of opabiniid-like euarthropods, each bearing an anterior proboscis (a fused protocerebral appendage), from the Middle Ordovician Castle Bank Biota, Wales, UK. Phylogenetic analyses support a paraphyletic grade of stem-group euarthropods with fused protocerebral appendages and a posterior-facing mouth, as in the iconic Cambrian panarthropod Opabinia. These results suggest that the labrum may have reduced from an already-fused proboscis, rather than a pair of arthropodized appendages. If some shared features between the Castle Bank specimens and radiodonts are considered convergent rather than homologous, phylogenetic analyses retrieve them as opabiniids, substantially extending the geographic and temporal range of Opabiniidae.
Topics: Animals; Phylogeny; Biological Evolution; Fossils; Arthropods; Head; Mouth
PubMed: 36379946
DOI: 10.1038/s41467-022-34204-w