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Tissue Engineering and Regenerative... Aug 2021Extracellular vesicles (EVs) are sub-micrometer lipid vesicles secreted from parental cells with their information such as DNA, RNA, and proteins. EVs can deliver their... (Review)
Review
Extracellular vesicles (EVs) are sub-micrometer lipid vesicles secreted from parental cells with their information such as DNA, RNA, and proteins. EVs can deliver their cargo to recipient cells and regulate the signaling pathway of the recipient cells to determine their destiny. Depending on the cargo of EVs, the recipient cells can be changed into abnormal state or be relieved from diseases. Therefore, EVs has been spotlighted as emerging therapeutics in biomedical research. However, slow EV secretion rate is the major limitation for the clinical applications of EVs. EV secretion is highly environmental dependent and can be regulated by various stimulants such as chemicals, oxygen levels, pH, radiation, starvation, and culture methods. To overcome the limitation of low productivity of EVs, EV stimulation methods have been widely studied and applied to massive EV productions. Another strategy is the synthesis of artificial EVs from cells by physical methods such as nitrogen cavitation, extrusion via porous membrane, and sonication. These physical methods disrupt cellular membrane and reassemble the membrane to lipid vesicles containing proteins or drugs. In this review, we will focus on how EV generation can be enhanced and recent advances in large scale EV generation strategies.
Topics: Biological Transport; Cell Membrane; Extracellular Vesicles; Signal Transduction; Sonication
PubMed: 34275103
DOI: 10.1007/s13770-021-00364-x -
Biomacromolecules Mar 2022Cellulose fibrils are the structural backbone of plants and, if carefully liberated from biomass, a promising building block for a bio-based society. The mechanism of...
Cellulose fibrils are the structural backbone of plants and, if carefully liberated from biomass, a promising building block for a bio-based society. The mechanism of the mechanical release─fibrillation─is not yet understood, which hinders efficient production with the required reliable quality. One promising process for fine fibrillation and total fibrillation of cellulose is cavitation. In this study, we investigate the cavitation treatment of dissolving, enzymatically pretreated, and derivatized (TEMPO oxidized and carboxymethylated) cellulose fiber pulp by hydrodynamic and acoustic (i.e., sonication) cavitation. The derivatized fibers exhibited significant damage from the cavitation treatment, and sonication efficiently fibrillated the fibers into nanocellulose with an elementary fibril thickness. The breakage of cellulose fibers and fibrils depends on the number of cavitation treatment events. In assessing the damage to the fiber, we presume that microstreaming in the vicinity of imploding cavities breaks the fiber into fibrils, most likely by bending. A simple model showed the correlation between the fibrillation of the carboxymethylated cellulose (CMCe) fibers, the sonication power and time, and the relative size of the active zone below the sonication horn.
Topics: Biomass; Carbohydrates; Cellulose; Oxidation-Reduction; Sonication
PubMed: 35099936
DOI: 10.1021/acs.biomac.1c01309 -
Molecules (Basel, Switzerland) Jun 2021Subcritical water refers to high-temperature and high-pressure water. A unique and useful characteristic of subcritical water is that its polarity can be dramatically... (Review)
Review
Subcritical water refers to high-temperature and high-pressure water. A unique and useful characteristic of subcritical water is that its polarity can be dramatically decreased with increasing temperature. Therefore, subcritical water can behave similar to methanol or ethanol. This makes subcritical water a green extraction fluid used for a variety of organic species. This review focuses on the subcritical water extraction (SBWE) of natural products. The extracted materials include medicinal and seasoning herbs, vegetables, fruits, food by-products, algae, shrubs, tea leaves, grains, and seeds. A wide range of natural products such as alkaloids, carbohydrates, essential oil, flavonoids, glycosides, lignans, organic acids, polyphenolics, quinones, steroids, and terpenes have been extracted using subcritical water. Various SBWE systems and their advantages and drawbacks have also been discussed in this review. In addition, we have reviewed co-solvents including ethanol, methanol, salts, and ionic liquids used to assist SBWE. Other extraction techniques such as microwave and sonication combined with SBWE are also covered in this review. It is very clear that temperature has the most significant effect on SBWE efficiency, and thus, it can be optimized. The optimal temperature ranges from 130 to 240 °C for extracting the natural products mentioned above. This review can help readers learn more about the SBWE technology, especially for readers with an interest in the field of green extraction of natural products. The major advantage of SBWE of natural products is that water is nontoxic, and therefore, it is more suitable for the extraction of herbs, vegetables, and fruits. Another advantage is that no liquid waste disposal is required after SBWE. Compared with organic solvents, subcritical water not only has advantages in ecology, economy, and safety, but also its density, ion product, and dielectric constant can be adjusted by temperature. These tunable properties allow subcritical water to carry out class selective extractions such as extracting polar compounds at lower temperatures and less polar ingredients at higher temperatures. SBWE can mimic the traditional herbal decoction for preparing herbal medication and with higher extraction efficiency. Since SBWE employs high-temperature and high-pressure, great caution is needed for safe operation. Another challenge for application of SBWE is potential organic degradation under high temperature conditions. We highly recommend conducting analyte stability checks when carrying out SBWE. For analytes with poor SBWE efficiency, a small number of organic modifiers such as ethanol, surfactants, or ionic liquids may be added.
Topics: Biological Products; Hot Temperature; Plant Extracts; Solvents; Sonication; Water
PubMed: 34209151
DOI: 10.3390/molecules26134004 -
Acta Orthopaedica Feb 2017
Topics: Canada; Editorial Policies; Fracture Healing; Humans; Periodicals as Topic; Sonication; Tibial Fractures
PubMed: 27998203
DOI: 10.1080/17453674.2016.1269273 -
Ultrasonics Sonochemistry Jun 2021Antibiotic residues in water are general health and environmental risks due to the antibiotic-resistance phenomenon. Sonication has been included among the advanced... (Review)
Review
Antibiotic residues in water are general health and environmental risks due to the antibiotic-resistance phenomenon. Sonication has been included among the advanced oxidation processes (AOPs) used to remove recalcitrant contaminants in aquatic environments. Sonochemical processes have shown substantial advantages, including cleanliness, safety, energy savings and either negligible or no secondary pollution. This review provides a wide overview of the different protocols and degradation mechanisms for antibiotics that either use sonication alone or in hybrid processes, such as sonication with catalysts, Fenton and Fenton-like processes, photolysis, ozonation, etc.
Topics: Anti-Bacterial Agents; Solutions; Sonication; Water; Water Pollutants, Chemical
PubMed: 33975189
DOI: 10.1016/j.ultsonch.2021.105566 -
Ultrasonics Sonochemistry Nov 2022Spherical SiO nanoparticles (SSNs) have been inventively synthesized using the Stöber method with sonication at medium-high frequencies (80, 120, and 500 kHz), aiming...
Spherical SiO nanoparticles (SSNs) have been inventively synthesized using the Stöber method with sonication at medium-high frequencies (80, 120, and 500 kHz), aiming to control SSN size and shorten reaction time. Compared to the conventional method, such sonication allowed the Stöber reaction complete in 20-60 min with a low molar ratio of NHOH/tetraethyl orthosilicate (0.84). The hydrodynamic diameters of 63-117 nm of SSNs were obtained under sonication with 80, 120, and 500 kHz of ultrasonic frequencies. Moreover, the SSNs obtained were smaller at 120 kHz than at 80 kHz in a multi-frequencies ultrasonic reactor, and the SSN size decreased with increasing ultrasonic power at 20 °C, designating the sonochemical unique character, namely, the SSN-size control is associated with the number of microbubbles originated by sonication. With another 500 kHz ultrasonic bath, the optimal system temperature for producing smaller SSNs was proven to be 20 °C. Also, the SSN size decreased with increasing ultrasonic power. The smallest SSNs (63 nm, hydrodynamic diameter by QELS, or 21 nm by FESEM) were obtained by sonication at 207 W for 20 min at 20 °C. Furthermore, the SSN size increased slightly with increasing sonication time and volume, favoring the scale-up of SSNs preparation. The mechanisms of controlling the SSN size were further discussed by the radical's role and effects of ammonia and ethanol concentration.
Topics: Sonication; Silicon Dioxide; Microbubbles; Nanoparticles; Temperature
PubMed: 36182836
DOI: 10.1016/j.ultsonch.2022.106181 -
Annual Review of Biomedical Engineering Jul 2021Specialized features of vasculature in the central nervous system greatly limit therapeutic treatment options for many neuropathologies. Focused ultrasound, in... (Review)
Review
Specialized features of vasculature in the central nervous system greatly limit therapeutic treatment options for many neuropathologies. Focused ultrasound, in combination with circulating microbubbles, can be used to transiently and noninvasively increase cerebrovascular permeability with a high level of spatial precision. For minutes to hours following sonication, drugs can be administered systemically to extravasate in the targeted brain regions and exert a therapeutic effect, after which permeability returns to baseline levels. With the wide range of therapeutic agents that can be delivered using this approach and the growing clinical need, focused ultrasound and microbubble (FUS+MB) exposure in the brain has entered human testing to assess safety. This review outlines the use of FUS+MB-mediated cerebrovascular permeability enhancement as a drug delivery technique, details several technical and biological considerations of this approach, summarizes results from the clinical trials conducted to date, and discusses the future direction of the field.
Topics: Blood-Brain Barrier; Brain; Drug Delivery Systems; Humans; Microbubbles; Sonication
PubMed: 33752471
DOI: 10.1146/annurev-bioeng-062117-121238 -
Molecules (Basel, Switzerland) Dec 2022Sonication is recognized as a potential food processing method to improve the functional properties of fruit juice. This study evaluated the effects of different...
Sonication is recognized as a potential food processing method to improve the functional properties of fruit juice. This study evaluated the effects of different sonication durations (20, 40, and 60 min) and thermal pasteurization on the nutritional, antioxidant, and microbial properties of noni juice. Fresh noni juice served as the control. The main organic acids detected were malic (57.54−89.31 mg/100 mL) and ascorbic (17.15−31.55 mg/100 mL) acids. Compared with the fresh sample, the concentrations of these compounds were significantly improved (p < 0.05) in the 60 min sonicated sample but reduced (p < 0.05) in the pasteurized sample. Moreover, sonication for 60 min resulted in increments of scopoletin, rutin, and vanillic acid compared to the fresh sample. The antioxidant activity of the juice sample was improved in the sample sonicated for 60 min. Irrespective of juice processing method, the level of microbial counts in noni juice was within the satisfactory level over the 8 weeks of refrigerated (4 °C) storage. This study highlights the feasibility of using ultrasound processing to enhance the quality of noni juice on the industrial scale.
Topics: Antioxidants; Morinda; Fruit; Pasteurization; Sonication
PubMed: 36615507
DOI: 10.3390/molecules28010313 -
International Journal of Pharmaceutics Dec 2021Sonodynamic therapy (SDT) is a new therapeutic modality for noninvasive cancer treatment based on the association of ultrasound and sonosensitizer drugs. Up to date,... (Review)
Review
Sonodynamic therapy (SDT) is a new therapeutic modality for noninvasive cancer treatment based on the association of ultrasound and sonosensitizer drugs. Up to date, there is not a consensus on the standardization of the experimental conditions for the in vitro studies to correctly assess cell viability during SDT. Therefore, this review article mainly describes how the main ultrasound parameters and experimental setups of ultrasound application in vitro studies can influence the SDT bioeffects/response. The sonodynamic action is impacted by the combination of frequency, intensity, duty cycle, and ultrasound application time. The variation of experimental setups in cell culture, such as the transducer position, cell-transducer distance, coupling medium thickness, or type of culture, also influences the sonodynamic response. The intensity, duty cycle, and sonication duration increase cytotoxicity and reactive oxygen species production. For similar ultrasound parameters, differences in the experimental configuration impact cell death in vitro. Four main experimental setups are used to assess for SDT in cell culture (i) a planar transducer placed directly in contact with the bottom of the culture microplate; (ii) microplate positioned in the transducer's far-field using a water tank; (iii) sealed cell culture tubes immersed in water away from the transducer; and (iv) transducer dipped directly into the well with cell culture. Because of the significant variations in the experimental setups, sonodynamic response can significantly vary, and the translation of these results for in vivo experimentation is difficult. Therefore, a well-designed and detailed in vitro experimental setup is vital for understanding the interactions among the biological medium, the sonosensitizer, and the ultrasound for the in vitro to in vivo translation in SDT.
Topics: Cell Line, Tumor; Cell Survival; Reactive Oxygen Species; Sonication; Ultrasonic Therapy; Ultrasonography
PubMed: 34743959
DOI: 10.1016/j.ijpharm.2021.121243 -
Scientific Reports Apr 2019Biomolecules for OMIC analysis of microbial communities are commonly extracted by bead-beating or ultra-sonication, but both showed varying yields. In addition to that,...
Biomolecules for OMIC analysis of microbial communities are commonly extracted by bead-beating or ultra-sonication, but both showed varying yields. In addition to that, different disruption pressures are necessary to lyse bacteria and fungi. However, the disruption efficiency and yields comparing bead-beating and ultra-sonication of different biological material have not yet been demonstrated. Here, we show that ultra-sonication in a bath transfers three times more energy than bead-beating over 10 min. TEM imaging revealed intact gram-positive bacterial and fungal cells whereas the gram-negative bacterial cells were destroyed beyond recognition after 10 min of ultra-sonication. DNA extraction using 10 min of bead-beating revealed higher yields for fungi but the extraction efficiency was at least three-fold lower considering its larger genome. By our critical viewpoint, we encourage the review of the commonly used extraction techniques as we provide evidence for a potential underrepresentation of resistant microbes, particularly fungi, in ecological studies.
Topics: Bacteria; Bacterial Proteins; DNA; DNA, Bacterial; Drug Resistance, Bacterial; Drug Resistance, Fungal; Fungal Proteins; Fungi; Microspheres; Sonication
PubMed: 30948770
DOI: 10.1038/s41598-019-42188-9