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Journal of Fungi (Basel, Switzerland) Sep 2022Three new species of are introduced based on specimens collected from China. sp. nov. is distinguished by pale yellow to pale orange-yellow perithecia with a very low...
Three new species of are introduced based on specimens collected from China. sp. nov. is distinguished by pale yellow to pale orange-yellow perithecia with a very low papilla, clavate to subcylindrical asci possessing ellipsoidal to elongate-ellipsoidal spinulose ascospores 13-16 × 4.5-5.5 μm; it has acremonium- to verticillium-like conidiophores and ellipsoidal to rod-shaped conidia. sp. nov. has pinkish-white subglobose to globose perithecia on a well-developed stroma and with a thin perithecial wall, clavate to subcylindrical asci with ellipsoidal to elongate-ellipsoidal spinulose ascospores 7.5-11 × 2.5-3.5 μm; it produces verticillium-like conidiophores and ellipsoidal to subellipsoidal conidia. sp. nov. features solitary to gregarious perithecia with a papilla, clavate asci containing 6-8 smooth-walled ascospores 9-17 × 3-5.5 μm; it forms verticillium-like conidiophores and sparse, subfusiform conidia. The morphological characteristics and phylogenetic analyses of combined nuclear ribosomal DNA ITS1-5.8S-ITS2 and beta-tubulin sequences support their placement in and their classification as new to science. Distinctions between the novel taxa and their close relatives are compared herein.
PubMed: 36294592
DOI: 10.3390/jof8101027 -
Open Life Sciences 2021The entomopathogenic fungus T011, parasitizing on nymph of Cicada, collected in the coffee garden in Dak Lak Province, Vietnam, was preliminarily morphologically...
The entomopathogenic fungus T011, parasitizing on nymph of Cicada, collected in the coffee garden in Dak Lak Province, Vietnam, was preliminarily morphologically identified as , belonged to order Hypocreales and family Clavicipitaceae. To ensure the authenticity of T011, phylogenetic analysis of the concatenated set of multiple genes including , , , , and was applied to support the identification. Genomic DNA was isolated from dried sample T011. The PCR assay sequencing was applied to amplify , , , , and gene. For phylogenetic analysis, the concatenated data of both target gens were constructed with MEGAX with a 1,000 replicate bootstrap based on the neighbor-joining, maximum likelihood, maximum parsimony method. As the result, the concatenated data containing 62 sequences belonged to order Hypocreales, families Clavicipitaceae, and 2 outgroup sequences belonged to order Hypocreales, genus . The phylogenetic analysis results indicated that T011 was accepted at subclade and significantly formed the monophyletic group with referent (Telemorph of ) with high bootstrap value. The phylogenetically analyzed result was strongly supported by our morphological analysis described as the . In summary, phylogenetic analyses based on the concatenated dataset were successfully applied to strengthen the identification of T011 as .
PubMed: 34307886
DOI: 10.1515/biol-2021-0074 -
Persoonia Jun 2015Stromata of Trichoderma species having green ascospores were collected in various regions of China. Based on morphology of the sexual and asexual morph, culture...
Stromata of Trichoderma species having green ascospores were collected in various regions of China. Based on morphology of the sexual and asexual morph, culture characteristics, and sequence analyses of rpb2 and tef1 genes, 17 species with green ascospores were identified. Among them, Trichoderma rosulatum, T. rufobrunneum and T. stipitatum are described as new species, and seven other species are reported for the first time from China. Trichoderma rosulatum produces small bright yellow or pale greenish stromata with dense dark green ostioles and gliocladium-like conidiophores, shows a close relationship to T. thelephoricola, and belongs to the Chlorospora clade. Trichoderma rufobrunneum, which typically forms reddish brown stromata, is recognised as a member of the Harzianum clade. Trichoderma stipitatum is characterised by turbinate, pale yellow to nearly orange stromata and verticillium-like conidiophores; it forms a distinct, independent lineage with strong bootstrap support in the phylogenetic trees. The distinctions between the new species and their close relatives are discussed, and their phylogenetic positions are explored.
PubMed: 26240449
DOI: 10.3767/003158515X686732 -
PeerJ 2022The peachfruit fly, (Saunders) is a polyphagous pest in nature, belonging to order, and their respective family is . It mostly feeds on different crops, vegetables and...
The peachfruit fly, (Saunders) is a polyphagous pest in nature, belonging to order, and their respective family is . It mostly feeds on different crops, vegetables and fruits. Different traditional chemical insecticides have been used to control this notorious pest. Excessive consumption of pesticides has become a major threat to the fresh fruits trade since many importing countries refused to accept the shipments due to public health and environmental concerns. There is a growing trend to control these pests using the most effective biological control methods and other preventive measures have been adopted for reducing their attacks. Fungal agents have been used as biological agents to manage the attack of different insects pest through biological means. The present study was conducted to assess the virulence of three entomopathogenic fungi, , and against stages under different laboratory conditions. The results showed that and were more effective in pathogenicity and potentially kill at all stages of as compared to . The highest mortality rate for the third larval instar and the pupal stage were recorded after exposure to the 1 × 10 conidia/ml concentrations, with 68.67% and 89.67%, respectively. Adult flies were the most susceptible to all entomopathogenic fungi. However, was more virulent against adult flies than and at 1 × 10 conidial concentration. Therefore, the entomopathogenic fungi and can be used as an cost effective bio-insecticide in the integrated pest management programs to control . This study will be helpful to overcome this pest through biological control means.
Topics: Animals; Tephritidae; Pest Control, Biological; Insecticides; Larva; Metarhizium; Spores, Fungal
PubMed: 35480558
DOI: 10.7717/peerj.13316 -
Mycology 2022The entomopathogenic fungus strain IMI 268317, previously known as and , is currently used as a microbial insecticide to protect tomatoes from serious leaf-inhabiting...
Entomopathogenic fungus (Hypocreales: Cordycipitaceae) strain IMI 268317 colonises on tomato leaf surface through conidial adhesion and general and microcycle conidiation.
The entomopathogenic fungus strain IMI 268317, previously known as and , is currently used as a microbial insecticide to protect tomatoes from serious leaf-inhabiting pests in greenhouses. However, its persistence on tomato leaves has been unidentified. Understanding the events and processes of phyllosphere colonisation by this strain should help in developing its practical applications. This study assessed the epiphytic abilities of this strain on tomato leaves in humid conditions, simulating closed greenhouse environments. Conidia applied on tomato leaflets strongly adhered 12 h after inoculation. The mucilage-like materials were found around the germinated conidia after 3 days after inoculation (dpi), which possibly strengthened the adhesion. A total of 15% of conidia germinated at 3 dpi, of which 2% formed typical conidium or an enlarged structure on germ-tube tips. Many conidia were produced on phialide tips that branched from elongated hyphae at 7 dpi; however, invasion into leaf tissue was not observed. On the leaflets, inoculated conidia suspensions of 1 × 10 and 1 × 10 conidia/mL, colony forming units increased 52.6 and 8.8 folds from 0 to 14 dpi, respectively. These results suggested that strain IMI 268317 has high epiphytic abilities on tomato leaflets in a humid condition.
PubMed: 35711329
DOI: 10.1080/21501203.2021.1944929 -
Scientific Reports Sep 2016Chinese Cordyceps, known in Chinese as "DongChong XiaCao", is a parasitic complex of a fungus (Ophiocordyceps sinensis) and a caterpillar. The current study explored the...
Chinese Cordyceps, known in Chinese as "DongChong XiaCao", is a parasitic complex of a fungus (Ophiocordyceps sinensis) and a caterpillar. The current study explored the endogenetic fungal communities inhabiting Chinese Cordyceps. Samples were collected from five different geographical regions of Qinghai and Tibet, and the nuclear ribosomal internal transcribed spacer-1 sequences from each sample were obtained using Illumina high-throughput sequencing. The results showed that Ascomycota was the dominant fungal phylum in Chinese Cordyceps and its soil microhabitat from different sampling regions. Among the Ascomycota, 65 genera were identified, and the abundant operational taxonomic units showed the strongest sequence similarity to Ophiocordyceps, Verticillium, Pseudallescheria, Candida and Ilyonectria Not surprisingly, the genus Ophiocordyceps was the largest among the fungal communities identified in the fruiting bodies and external mycelial cortices of Chinese Cordyceps. In addition, fungal communities in the soil microhabitats were clustered separately from the external mycelial cortices and fruiting bodies of Chinese Cordyceps from different sampling regions. There was no significant structural difference in the fungal communities between the fruiting bodies and external mycelial cortices of Chinese Cordyceps. This study revealed an unexpectedly high diversity of fungal communities inhabiting the Chinese Cordyceps and its microhabitats.
Topics: Base Sequence; Cluster Analysis; Cordyceps; DNA, Ribosomal Spacer; Ecosystem; Fruiting Bodies, Fungal; Genetic Variation; High-Throughput Nucleotide Sequencing; Hypocreales; Mycelium; Principal Component Analysis
PubMed: 27625176
DOI: 10.1038/srep33437 -
Molecules (Basel, Switzerland) Oct 2021In the process of screening for new bioactive microbial metabolites we found a novel ƴ-pyrone derivative for which we propose the trivial name luteapyrone, in a...
In the process of screening for new bioactive microbial metabolites we found a novel ƴ-pyrone derivative for which we propose the trivial name luteapyrone, in a recently described microscopic filamentous fungus, BiMM-F96/DF4. The compound was isolated from the culture extract of the fungus grown on modified yeast extract sucrose medium by means of flash chromatography followed by preparative HPLC. The chemical structure was elucidated by NMR and LC-MS. The new compound was found to be non-cytotoxic against three mammalian cell lines (HEK 263, KB-3.1 and Caco-2). Similarly, no antimicrobial activity was observed in tested microorganisms (gram positive and negative bacteria, yeast and fungi).
Topics: Fungi; Hypocreales; Molecular Structure
PubMed: 34770997
DOI: 10.3390/molecules26216589