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Chemistry (Weinheim An Der Bergstrasse,... Jul 2023Benefiting from the features like polymeric linear structures, stimuli-responsiveness and dynamic adaptiveness, supramolecular polymers (SPs) are favored as exploiting...
Benefiting from the features like polymeric linear structures, stimuli-responsiveness and dynamic adaptiveness, supramolecular polymers (SPs) are favored as exploiting muscle-like materials, allowing for imitating the muscle functions. However, a substantial part of these materials barely owned an unitary motion orientation while it was obviously known that muscle movements involved distinct orientations. Herein, M1 holding the structure of 44-membered macrocycle with two aldehyde groups was designed, meanwhile, M2 comprising of secondary ammonium ions, 3,5-di-tert-butylphenyl groups as well as the alkyl chains was fabricated, for which it could be assembled with M1 to generate SPs based on host-guest interactions from a large macrocycle and two secondary ammonium ions. SPs underwent vertical compression upon the addition of N H owing to the forming dynamic covalent bonds, notably, mechanically interlocked structures were also generated. Afterwards, the vertically compressed SPs experienced horizontal shrinkage when tetrabutylammonium chloride was contributed due to the destruction of host-guest interactions.
PubMed: 37158473
DOI: 10.1002/chem.202301313 -
Environmental Science and Pollution... Aug 2023Waste printed circuit boards (WPCBs) contain a plethora of valuable metals, considered an attractive secondary resource. In the current research, a hydrometallurgical...
Waste printed circuit boards (WPCBs) contain a plethora of valuable metals, considered an attractive secondary resource. In the current research, a hydrometallurgical process combined ammonia/ammonium chloride leaching and reduction (using L-ascorbic acid) to recover copper and its oxide (CuO) as nanosized particles from WPCBs was investigated. The results of leaching indicated that 96.7% of copper could be recovered at a temperature of 35 °C for a leaching duration of 2 h with ammonium chloride and ammonia concentration of 2 mol/L at a solid:liquid ratio of 1:10 g/cm. The synthesized particles exhibit spherical and distorted sphere morphology with average particle size of 460 nm and 50 nm for Cu and CuO NPs, respectively. The antibacterial activity of Cu, CuO, and a (1:1) blend of both (Cu/CuO) has been examined against five different bacterial and fungal strains. The highest zone of inhibition was measured as 21.2 mm for Cu NPs toward Escherichia coli and 16.7 mm for Cu/CuO blend toward Bacillus cereus bacteria. The highest zone of inhibition was measured as 13 mm and 13.8 mm for Cu/CuO blend toward Fusarium proliferatum and Penicillium verrucosum fungi. Cu/CuO blend showed notable photocatalytic activity towards Rhodamine B dye under visible light irradiation with 96% degradation rate within 120 min. Using the process developed in this study, copper and its oxide as nanoparticles can be produced from WPCBs and used for multifunctional applications.
Topics: Copper; Electronic Waste; Ammonia; Ammonium Chloride; Nanoparticles; Fungi; Anti-Bacterial Agents; Oxides; Metal Nanoparticles
PubMed: 37458881
DOI: 10.1007/s11356-023-28437-5 -
Journal of Hazardous Materials Jul 2023Microplastics (MPs) contamination in soils seriously threatens agroecosystems globally. However, very few studies have been done on the effects of MPs on the soil...
Microplastics (MPs) contamination in soils seriously threatens agroecosystems globally. However, very few studies have been done on the effects of MPs on the soil nitrogen cycle and related functional microorganisms. To assess MP's impact on the soil nitrogen cycle and related functional bacteria, we carried out a one-month soil incubation experiment using typical acidic soil. The soil was amended with alfalfa meal and was spiked with 1% and 5% (mass percentage) of low-density polyethylene (LDPE) and polyvinyl chloride (PVC) MPs. Our results showed that both LDPE and PVC addition significantly increased soil nitrification rate and nitrate reductase activity, which could further promote soil denitrification. The relative abundance of diazotrophs, ammonium oxidizing, and denitrifying bacterial groups were significantly altered with MPs addition. Moreover, the MPs treatments greatly enhanced denitrifying bacteria richness. Redundancy analysis showed that nitrate reductase activity was the most significant factor affecting the soil functional bacterial community. Correlation analysis shows that Nitrosospira genus might be for the improvement of soil nitrification rate. Our results implied that MPs exposure could significantly affect the soil nitrogen cycling in farmland ecosystems by influencing essential nitrogen functional microorganisms and related enzymatic activities.
Topics: Nitrification; Polyethylene; Microplastics; Plastics; Polyvinyl Chloride; Ecosystem; Soil; Nitrogen; Bacteria; Nitrate Reductases; Soil Microbiology
PubMed: 37043864
DOI: 10.1016/j.jhazmat.2023.131391 -
Frontiers in Bioengineering and... 2023Ammonia (Amm), and its aqueous solved state, ammonium, which is produced from glutamine (Gln) metabolism, is a known inhibitor of stem cell proliferation . In the...
Ammonia (Amm), and its aqueous solved state, ammonium, which is produced from glutamine (Gln) metabolism, is a known inhibitor of stem cell proliferation . In the context of cultivated beef, primary bovine fibro-adipogenic progenitor cells (FAPs) need to be grown and differentiated for several weeks for the production of cultivated fat. In this study, the ammonium sensitivity of these cells was investigated by introducing ammonium chloride, which was found to inhibit their proliferation when above 5 mM and their adipogenic differentiation when above 2 mM. Novel serum-free proliferation and differentiation media were hence developed with the aim to suppress Amm production during expansion and adipogenesis. Glutamine substitutes, such as a-ketoglutarate (aKG), glutamate (Glt) and pyruvate (Pyr) were investigated. It was found that aKG based proliferation medium (PM) was the most effective in promoting and maintaining FAPs growth over several passages while the specific Amm production rate was reduced more than 5-fold. In terms of differentiation capacity, the substitution of glucose (Gluc) and Gln with galactose (Gal) and Pyr was shown to be the most effective in promoting FAPs differentiation into mature adipocytes, resulting in over 2-fold increase of fat volume per cell, while suppressing Amm production. Our findings suggest that FAPs do not require Gln as an essential nutrient but, on the contrary, possess all the necessary metabolic pathways to proliferate and subsequently differentiate in a Gln-free medium, resulting in decreased Amm production rates and seemingly synthesising glutamine . These findings are important for prolonging the lifespan of culture medium, allowing for reduced costs and process interventions.
PubMed: 37555077
DOI: 10.3389/fbioe.2023.1202165 -
Environmental Toxicology and Chemistry Aug 2023While it is recognized that groundwater contaminant plumes can impact surface waters, there remains little information on the magnitude, spatial extent, and especially...
While it is recognized that groundwater contaminant plumes can impact surface waters, there remains little information on the magnitude, spatial extent, and especially temporal variability of the resulting exposure to the variety of aquatic organisms, particularly for stagnant surface waters (e.g., ponds). The present study of a historic landfill plume discharging to a pond investigated contaminant exposure to multiple aquatic zones (endobenthic, epibenthic, pelagic) over approximately 1 year within a temperate climate. Landfill tracers included the artificial sweetener saccharin, ammonium, chloride, and specific conductance. Sampling of pond sediment porewater (upwelling groundwater) and continuous geophysical imaging of the subsurface showed a relatively stable plume footprint covering approximately 26% of the pond, although with spatially varying leachate composition, revealing year-round exposure to endobenthic (within sediments) organisms. Substantial and variable contaminant exposure to epibenthic organisms within the plume footprint was shown by elevated specific conductance measured directly above the sediment interface. Exposure varied daily at times and increased through winter to values representing undiluted plume groundwater. Exposure to pelagic organisms (overlying water) covered a larger area (~50%) due to in-pond circulation. The stream outlet concentrations were stable at approximately 10 times dilution for chloride and saccharin, but were substantially less in summer for ammonium due to in-pond processes. Whereas groundwater contaminants are typically assumed elevated at base flows, the outlet stream contaminant mass discharges to downstream receptors were notably higher in winter than summer, following stream flow patterns. Insights from the present study into the timings and locations of contaminant plume exposure to multiple ecological zones of a pond can provide guidance to contaminated site and aquatic ecosystem managers on improved monitoring, assessment, and remediation protocols. Environ Toxicol Chem 2023;42:1667-1684. © 2023 His Majesty the King in Right of Canada and The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC. Reproduced with the permission of the Minister of Environment and Climate Change Canada.
Topics: Ponds; Ecosystem; Saccharin; Chlorides; Water Pollutants, Chemical; Groundwater; Ammonium Compounds; Waste Disposal Facilities; Environmental Monitoring
PubMed: 37194992
DOI: 10.1002/etc.5650 -
The Science of the Total Environment Dec 2023Lignin-based flocculants are widely used for wastewater purification, but their application in sludge dewatering has not yet been documented. In this study, a novel...
Lignin-based flocculants are widely used for wastewater purification, but their application in sludge dewatering has not yet been documented. In this study, a novel cationic lignin-based flocculant named LS-g-CPA was prepared by grafting cationic polyacrylamide (CPA) synthesized from methacryloyloxy ethyltrimethyl ammonium chloride (DMC) and acrylamide (AM) onto sodium lignosulfonate (LS), and its roles and underlying mechanisms in sludge conditioning were investigated. The results showed that LS-g-CPA effectively improved the dewaterability of sludge, reducing the filtration resistance and filter cake moisture content of sludge from 0.61 ± 0.05 × 10 m/kg to 0.14 ± 0.02 × 10 m/kg and 85.64 ± 0.25 % to 76.84 ± 0.41 %, respectively. The dewatering performance of LS-g-CPA was positively correlated with the DMC/AM ratio. The quaternary ammonium groups brought by DMC disrupted the reticular structure of extracellular polymeric substances, exposing hydrophobic residues and releasing bound water. Nevertheless, the key to LS-g-CPA for improving sludge dewatering lies more in the amphoteric flocculant properties that enhance sludge flocculation and the octopus-type structure that provides good drainage channels. This study reveals that lignin-based flocculants are effective in improving the dewaterability of sludge, which provides direct evidence for their application in sludge dewatering.
PubMed: 37696410
DOI: 10.1016/j.scitotenv.2023.166958 -
Journal of Environmental Management Sep 2023Landfill leachate (LL) management is an urgent issue at recently closed Sisdol Landfill Site (SLS) used to dispose of solid waste generated in Kathmandu (Nepal) as...
Landfill leachate (LL) management is an urgent issue at recently closed Sisdol Landfill Site (SLS) used to dispose of solid waste generated in Kathmandu (Nepal) as untreated leachate is flowing directly to the nearby Kolpu River causing environmental and health concerns. This study aims to assess the potential of algae-based treatment of LL pretreated by optimized coagulation-flocculation (CF) for the removal of conventional pollutants such as biological oxygen demand (BOD), chemical oxygen demand (COD), ammonia, nitrate, and phosphate. Response Surface Methodology (RSM) was used to optimize the operating variables (dose and pH) during the pretreatment of leachate by the CF process using ferric chloride (FeCl.7HO), alum (Al(SO).6HO) and commercial poly aluminium chloride (PAC) as coagulants using a jar test apparatus. The pretreated LL was subjected to algal treatment using the mixed microalgae culture isolated and enriched from the wastewater collection pond and grown in artificial light. The combined physicochemical and algal treatment of LL from SLS achieved 62.93-72.43%, 74.93-75.55% and 87.58-93.40% and 73.63-86.73% removal for COD, BOD, ammonium-nitrogen and phosphate, respectively. Thus, this research has proven the feasibility of a combined physiochemical and algae-based treatment of LL and also offers an exciting alternative to current treatment practices for LL.
Topics: Water Pollutants, Chemical; Flocculation; Wastewater; Phosphates; Nepal
PubMed: 37270978
DOI: 10.1016/j.jenvman.2023.118223 -
Langmuir : the ACS Journal of Surfaces... Apr 2024Interactions of anions with hydrophobic surfaces of proteins and water-soluble polymers depend on the ability of the ions to shed their hydration shells. At positively...
Interactions of anions with hydrophobic surfaces of proteins and water-soluble polymers depend on the ability of the ions to shed their hydration shells. At positively charged surfactant monolayers, the interactions of anions are less well understood. Due to the interplay of electrostatic surface forces, hydration effects, and ion-ion interactions in the electrostatic double layer, a comprehensive microscopic picture remains elusive. Herein, we study the interactions of chloride, bromide, and a mixture of these two anions at the aqueous interface of dihexadecyldimethylammonium (DHDA) and dioctadecyldimethylammonium (DODA) cationic monolayers. Using molecular dynamics simulations and three surface-sensitive X-ray scattering techniques, we demonstrate that bromide interacts preferentially over chloride with both monolayers. The structure of the two monolayers and their interfacial electron density profiles obtained from the simulations quantitatively reproduce the experimental data. We observe that chloride and bromide form contact ion pairs with the quaternary ammonium groups on both monolayers. However, ion pairing with bromide leads to a greater reduction in the number of water molecules hydrating the anion, resulting in more energetically stable ion pairs. This leads to long-range (>3 nm) lateral correlations between bromide ions on the structured DODA monolayer. These observations indicate that ion hydration is the dominant factor determining the interfacial electrolyte structure.
PubMed: 38502024
DOI: 10.1021/acs.langmuir.3c04003 -
Tree Physiology Feb 2024Nitrogen (N) plays an important role in mitigating salt stress in tree species. We investigate the genotypic differences in the uptake of ammonium (NH4+) and nitrate...
Nitrogen (N) plays an important role in mitigating salt stress in tree species. We investigate the genotypic differences in the uptake of ammonium (NH4+) and nitrate (NO3-) and the importance for salt tolerance in two contrasting poplars, salt-tolerant Populus euphratica Oliv. and salt-sensitive P. simonii × (P. pyramidalis ×Salix matsudana) (P. popularis cv. 35-44, P. popularis). Total N content, growth and photosynthesis were significantly reduced in P. popularis after 7 days of exposure to NaCl (100 mM) supplied with 1 mM NH4+ and 1 mM NO3-, while the salt effects were not pronounced in P. euphratica. The 15NH4+ trace and root flux profiles showed that salt-stressed poplars retained ammonium uptake, which was related to the upregulation of ammonium transporters (AMTs) in roots, as two of the four AMTs tested significantly increased in salt-stressed P. euphratica (i.e., AMT1.2, 2.1) and P. popularis (i.e., AMT1.1, 1.6). It should be noted that P. euphratica differs from salt-sensitive poplar in the maintenance of NO3- under salinity. 15NO3- tracing and root flux profiles showed that P. euphratica maintained nitrate uptake and transport, while the capacity to uptake NO3- was limited in salt-sensitive P. popularis. Salt increased the transcription of nitrate transporters (NRTs), NRT1.1, 1.2, 2.4, 3.1, in P. euphratica, while P. popularis showed a decrease in the transcripts of NRT1.1, 2.4, 3.1 after 7 days of salt stress. Furthermore, salt-stimulated transcription of plasmalemma H+-ATPases (HAs), HA2, HA4 and HA11 contributed to H+-pump activation and NO3- uptake in P. euphratica. However, salt stimulation of HAs was less pronounced in P. popularis, where a decrease in HA2 transcripts was observed in the stressed roots. We conclude that the salinity-decreased transcripts of NRTs and HAs reduced the ability to uptake NO3- in P. popularis, resulting in limited nitrogen supply. In comparison, P. euphratica maintains NH4+ and NO3- supply, mitigating the negative effects of salt stress.
Topics: Nitrates; Sodium Chloride; Populus; Plant Roots; Ammonium Compounds; Membrane Transport Proteins; Proton-Translocating ATPases; Nitrogen
PubMed: 38366380
DOI: 10.1093/treephys/tpae020 -
Advanced Materials (Deerfield Beach,... Mar 2024Polyester nanofiltration membranes highlight beneficial chlorine resistance, but their loose structures and negative charge result in poor cations retention precluding...
Polyester nanofiltration membranes highlight beneficial chlorine resistance, but their loose structures and negative charge result in poor cations retention precluding advanced use in cations separation. This work designs a new monomer (TET) containing "hydroxyl-ammonium" entities that confer dense structures and positive charge to polyester nanofiltration membranes. The TET monomer undergoes efficient interfacial polymerization with the trimesoyl chloride (TMC) monomer, and the resultant TET-TMC membranes feature one of the lowest molecular weight cut-offs (389 Da) and the highest zeta potential (4 mv, pH: 7) among all polyester nanofiltration membranes. The MgCl rejection of the TET-TMC membrane is 95.5%, significantly higher than state-of-the-art polyester nanofiltration membranes (<50%). The Li /Mg separation performance of TET-TMC membrane is on par with cutting-edge polyamide membranes, while additionally, the membrane is stable against NaClO though polyamide membranes readily degrade. Thus the TET-TMC is the first polyester nanofiltration membrane for efficient cations separation.
PubMed: 37907065
DOI: 10.1002/adma.202309406