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Mass Spectrometry Reviews 2024Mass spectrometry (MS) has become a central technique in cancer research. The ability to analyze various types of biomolecules in complex biological matrices makes it... (Review)
Review
Mass spectrometry (MS) has become a central technique in cancer research. The ability to analyze various types of biomolecules in complex biological matrices makes it well suited for understanding biochemical alterations associated with disease progression. Different biological samples, including serum, urine, saliva, and tissues have been successfully analyzed using mass spectrometry. In particular, spatial metabolomics using MS imaging (MSI) allows the direct visualization of metabolite distributions in tissues, thus enabling in-depth understanding of cancer-associated biochemical changes within specific structures. In recent years, MSI studies have been increasingly used to uncover metabolic reprogramming associated with cancer development, enabling the discovery of key biomarkers with potential for cancer diagnostics. In this review, we aim to cover the basic principles of MSI experiments for the nonspecialists, including fundamentals, the sample preparation process, the evolution of the mass spectrometry techniques used, and data analysis strategies. We also review MSI advances associated with cancer research in the last 5 years, including spatial lipidomics and glycomics, the adoption of three-dimensional and multimodal imaging MSI approaches, and the implementation of artificial intelligence/machine learning in MSI-based cancer studies. The adoption of MSI in clinical research and for single-cell metabolomics is also discussed. Spatially resolved studies on other small molecule metabolites such as amino acids, polyamines, and nucleotides/nucleosides will not be discussed in the context.
Topics: Humans; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Artificial Intelligence; Metabolomics; Neoplasms; Lipidomics
PubMed: 36065601
DOI: 10.1002/mas.21804 -
Journal of Gynecology Obstetrics and... Nov 2023The French National College of Obstetricians and Gynecologists (CNGOF) published guidelines for managing endometriosis-associated pain in 2018. Given the development of...
The French National College of Obstetricians and Gynecologists (CNGOF) published guidelines for managing endometriosis-associated pain in 2018. Given the development of new pharmacological therapies and a review that was published in 2021, most national and international guidelines now suggest a new therapeutic approach. In addition, a novel validated screening method based on patient questionnaires and analysis of 109-miRNA saliva signatures, which combines biomarkers and artificial intelligence, opens up new avenues for overcoming diagnostic challenges in patients with pelvic pain and for avoiding laparoscopic surgery when sonography and MRI are not conclusive. Dienogest (DNG) 2 mg has been a reimbursable healthcare expense in France since 2020, and, according to recent studies, it is at least as effective as combined hormonal contraception (CHC) and can be used as an alternative to CHC for first-line treatment of endometriosis-associated pain. Since 2018, the literature concerning the use of DNG has grown considerably, and the French guidelines should be modified accordingly. The levonorgestrel intrauterine system (LNG IUS) and other available progestins per os, including DNG, or the subcutaneous implant, can be offered as first-line therapy, gonadotropin-releasing hormone (GnRH) agonists with add-back therapy (ABT) as second-line therapy. Oral GnRH antagonists are promising new medical treatments for women with endometriosis-associated pain. They competitively bind to GnRH receptors in the anterior pituitary, preventing native GnRH from binding to GnRH receptors and from stimulating the secretion of luteinizing hormone and follicle-stimulating hormone. Consequently, estradiol and progesterone production is reduced. Oral GnRH antagonists will soon be on the market in France. Given their mode of action, their efficacy is comparable to that of GnRH agonists, with the advantage of oral administration and rapid action with no flare-up effect. Combination therapy with ABT is likely to allow long-term treatment with minimal impact on bone mass. GnRH antagonists with ABT may thus be offered as second-line treatment as an alternative to GnRH agonists with ABT. This article presents an update on the management of endometriosis-associated pain in women who do not have an immediate desire for pregnancy.
Topics: Female; Humans; Endometriosis; Receptors, LHRH; Artificial Intelligence; Pelvic Pain; Gonadotropin-Releasing Hormone; Hormone Antagonists
PubMed: 37669732
DOI: 10.1016/j.jogoh.2023.102664 -
Pharmaceuticals (Basel, Switzerland) Aug 2023Xerostomia, commonly known as dry mouth, is a widespread oral health malfunction characterized by decreased salivary flow. This condition results in discomfort, impaired... (Review)
Review
Xerostomia, commonly known as dry mouth, is a widespread oral health malfunction characterized by decreased salivary flow. This condition results in discomfort, impaired speech and mastication, dysphagia, heightened susceptibility to oral infections, and ultimately, a diminished oral health-related quality of life. The etiology of xerostomia is multifaceted, with primary causes encompassing the use of xerostomic medications, radiation therapy to the head and neck, and systemic diseases such as Sjögren's syndrome. Consequently, there is a growing interest in devising management strategies to address this oral health issue, which presents significant challenges due to the intricate nature of saliva. Historically, natural products have served medicinal purposes, and in contemporary pharmaceutical research and development, they continue to play a crucial role, including the treatment of xerostomia. In this context, the present review aims to provide an overview of the current state of knowledge regarding natural compounds and extracts for xerostomia treatment, paving the way for developing novel therapeutic strategies for this common oral health issue.
PubMed: 37631049
DOI: 10.3390/ph16081136 -
Oral Diseases Nov 2023Radiation-induced dry mouth negatively impacts patients' oral health and quality of life. Currently, evidence-based recommendation for non-pharmacologic interventions is... (Review)
Review
OBJECTIVES
Radiation-induced dry mouth negatively impacts patients' oral health and quality of life. Currently, evidence-based recommendation for non-pharmacologic interventions is still lacking. This study aimed to systematically review clinical trials evaluating the efficacy of non-pharmacologic interventions in cancer patients with radiation-induced dry mouth.
METHODS
Randomized controlled trials from 2000 were searched from 4 databases, including MEDLINE, Cochrane, Embase via OVID, and SCOPUS, up to December 16th, 2022 (PROSPERO registration CRD42022378405). The risk of bias was assessed using the revised Cochrane risk of bias assessment tool.
RESULTS
Twenty-one studies were included: 11 on artificial saliva, 4 on electrical nerve stimulation (TENS), 2 on acupuncture, and one study each on low-level laser therapy, stem cells, chewing gum, and probiotics. Overall bias was low, medium, and high in 33%, 48%, and 19% of the studies, respectively. Certain artificial saliva products and TENS were shown to improve dry mouth symptoms and salivary flow rate (SFR). One study showed that stem cell transplantation significantly increased SFR.
CONCLUSIONS
The evidence suggested that certain artificial saliva products and TENS are promising management. However, the evidence was still limited due to heterogeneity of interventions and outcome measurements. Thus, future studies using standard measurements and long-term follow-up are warranted.
PubMed: 37946598
DOI: 10.1111/odi.14804 -
Materials (Basel, Switzerland) Oct 2023The pitting corrosion of orthodontic apparatus elements in the oral environment is an interest of both clinicians and scientists dealing with the assessment of the...
The pitting corrosion of orthodontic apparatus elements in the oral environment is an interest of both clinicians and scientists dealing with the assessment of the biocompatibility of medical materials. This work presents a study on the effect of ready-to-use Listerine and Meridol mouthwashes and sodium fluoride on the resistance of the commercial Remanium-type orthodontic archwire to pitting corrosion in artificial saliva at 37 °C. XRD, SEM, EDS, mechanical properties, and microhardness measurements were used to characterize the archwire. The in vitro corrosion resistance of the archwire was examined using the open-circuit potential method, electrochemical impedance spectroscopy, and anodic polarization curves. The physicochemical characteristics confirmed the presence of a bi-phase alloy with a mixed austenite/ferrite structure containing Fe 74.4(7) at.%, Cr 18.4(4) at.%, and Ni 7.2(4) at.%. The Fe-Cr-Ni alloy was characterized by high tensile strength and Vickers microhardness. EIS revealed the capacitive behavior with high corrosion resistance. It was found that the kinetics of pitting corrosion in the artificial saliva decreased in the presence of NaF and mouthwashes. The potentiodynamic characteristics confirmed the decrease in susceptibility to pitting corrosion after the modification of artificial saliva. The pitting corrosion mechanism of the self-passive oxide layer on the surface of the Fe-Cr-Ni electrode in the biological environment containing chloride ions was discussed in detail. Mechanical properties after corrosion tests were weakened.
PubMed: 37895772
DOI: 10.3390/ma16206791 -
European Journal of Translational... Jul 2023The purpose of this study was to investigate the effect of different solutions on the optical behavior of two types of 3D-printed resin (Detax and Asiga). The samples...
The purpose of this study was to investigate the effect of different solutions on the optical behavior of two types of 3D-printed resin (Detax and Asiga). The samples were randomly divided into 4 groups of cola, orange juice, tea and artificial saliva (control group) in the form of 14 x 10 mm blocks with a thickness of 1.5 ± 0.3 mm (10 samples in each group). By spectrophotometric device, the color of the examined samples was recorded in the CIE system and in three parameters *L (brightness), a* (red-green) and b* (blue-yellow) as the initial record. The color difference of the samples was recorded on 7 and 30 days after immersion as a secondary record. After that, using the formula, the color difference (ΔE) were calculated. T-test also was used to analyze the data. There was no significant difference between the amount of ΔE on 7th and 30th days after exposure to artificial saliva and cola and on 7th days after exposure to orange juice and tea. But on the 30th day, a significant difference was found in orange juice and tea drinks (p<0.05), so that the value of ΔE in Asiga printer was lower than Detax. ΔE changes in orange juice solution were more than other drinks. On the 30th day of immersion in tea drinks and orange juice, Asiga printer had significantly less color difference than Detax printer.
PubMed: 37767891
DOI: 10.4081/ejtm.2023.11493 -
NEJM Evidence Jul 2023BACKGROUND: The discovery of a saliva-based micro–ribonucleic acid (miRNA) signature for endometriosis in 2022 opened up new perspectives for early and noninvasive...
BACKGROUND: The discovery of a saliva-based micro–ribonucleic acid (miRNA) signature for endometriosis in 2022 opened up new perspectives for early and noninvasive diagnosis of the disease. The 109-miRNA saliva signature is the product of miRNA biomarkers and artificial intelligence (AI) modeling. We designed a multicenter study to provide external validation of its diagnostic accuracy. We present here an interim analysis. METHODS: The first 200 patients included in the multicenter prospective ENDOmiRNA Saliva Test study (NCT05244668) were included for interim analysis. The study population comprised women from 18 to 43 years of age with a formal diagnosis of endometriosis or with suspected endometriosis. Epidemiologic, clinical, and saliva sequencing data were collected between November 2021 and March 2022. Genomewide miRNA expression profiling by small RNA sequencing using next-generation sequencing (NGS) was performed, and a random forest algorithm was used to assess the diagnostic accuracy. RESULTS: In this interim analysis of the external validation cohort, with a population prevalence of 79.5%, the 109-miRNA saliva diagnostic signature for endometriosis had a sensitivity of 96.2% (95% confidence interval [CI], 93.7 to 97.3%), specificity of 95.1% (95% CI, 85.2 to 99.1%), positive predictive value of 95.1% (95% CI, 85.2 to 99.1%), negative predictive value of 86.7% (95% CI, 77.6 to 90.3%), positive likelihood ratio of 19.7 (95% CI, 6.3 to 108.8), negative likelihood ratio of 0.04 (95% CI, 0.03 to 0.07), and area under the receiver operating characteristic curve of 0.96 (95% CI, 0.92 to 0.98). CONCLUSIONS: The use of NGS and AI in the sequencing and analysis of miRNA provided a saliva-based miRNA signature for endometriosis. Our interim analysis of a prospective multicenter external validation study provides support for its ongoing investigation as a diagnostic tool. (Funded by Ziwig and the Conseil Régional d’Ile de France [Grant EX024087]; ClinicalTrials.gov number, NCT05244668.)
Topics: Female; Humans; MicroRNAs; Endometriosis; Prospective Studies; Reproducibility of Results; Biomarkers, Tumor
PubMed: 38320163
DOI: 10.1056/EVIDoa2200282 -
ACS Central Science Jul 2023
PubMed: 37521799
DOI: 10.1021/acscentsci.3c00803 -
Journal of Prosthodontics : Official... May 2024To examine the color stability of 3D-printed and milled, interim, and definitive, restorative materials after immersion in artificial saliva and wine for 1, 3, and 6...
PURPOSE
To examine the color stability of 3D-printed and milled, interim, and definitive, restorative materials after immersion in artificial saliva and wine for 1, 3, and 6 months.
MATERIAL AND METHODS
The study used a 2 × 5 factorial design with 10 subgroups, including 2 immersion liquids (artificial saliva and wine) and 5 manufacturing technology and restorative material combinations (n = 10). Color measurements were taken using a contact-type digital spectrophotometer (CM-2600d Spectrophotometer; Konica Minolta Healthcare Americas Inc) before immersion and at 1 month (T1), 3 months (T3), and 6 months (T6) after immersion. The CIE2000 system was used to calculate quantitative measurements of color differences in ΔE, and comparisons were made to the acceptability threshold (AT) and perceptibility threshold (PT). Repeated measures of ANOVA (α = 0.05) were used to compare differences in color changes between manufacturing technology/restorative material-immersion liquid combinations at T1, T3, and T6.
RESULTS
To compare the effect of immersion liquid and time on the manufacturing technology/restorative material groups, the ΔE values were compared to the PT of 0.8 and the AT of 1.8. Wine caused significant color changes in ΔE values beyond the PT and AT values in all groups at all time intervals, except for the AT value of milled definitive crowns (hybrid nano-ceramic material). Wine immersion caused significant ΔE for all manufacturing technology/restorative material groups at all time intervals (1 month, 3 months, and 6 months) when compared to artificial saliva immersion (all p < 0.001).
CONCLUSION
Upon exposure to artificial saliva, 80%-100% of samples from all groups remained within the acceptable and perceptible color change thresholds. The wine had significant chromogenic effects on all tested restorative materials, however, the milled definitive crowns (hybrid nano-ceramic material) showed the greatest color stability. For patients with heavy wine consumption, 3D-printed definitive crowns (hybrid ceramic-filled material) may show discoloration exceeding acceptable and perceptible color change limits.
PubMed: 38742795
DOI: 10.1111/jopr.13868