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The Science of the Total Environment Dec 2023Antibiotic resistance genes (ARGs), emerging environmental contaminants, have become challenges of public health security. However, the distribution and drivers of ARGs,...
Antibiotic resistance genes (ARGs), emerging environmental contaminants, have become challenges of public health security. However, the distribution and drivers of ARGs, especially high-risk ARGs, in large-scale aquaculture sediments remain unknown. Here, we collected sediment samples from 40 crayfish ponds in seven main crayfish culture provinces in China and then investigated the distribution and risk of ARGs based on high-throughput sequencing and quantitative PCR techniques. Our results suggested that aquaculture sediment was potential reservoir of ARGs and the abundance of aadA-02 was the highest. High-risk ARG (floR) was also prevalent in the sediment and was the most abundant in Jiangsu Province, where opportunistic pathogens were also enriched. The abundance of floR was positively correlated with different environmental factors, such as total phosphorus in water and total carbon in sediment. In addition, Mycobacterium sp., opportunistic pathogenic bacteria, might be potential host for floR. Furthermore, the potential propagation pathway of ARGs was from sediment to crayfish gut, and Bacteroidetes and Proteobacteria might be the main bacterial groups responsible for the proliferation of ARGs. Generally, our results illustrate that pond sediment may be an ARG reservoir of aquatic animals. Meanwhile, our study helps develop valuable strategies for accessing risks and managing ARGs.
Topics: Animals; Anti-Bacterial Agents; Genes, Bacterial; Drug Resistance, Microbial; Bacteria; Aquaculture; Astacoidea; China
PubMed: 37714353
DOI: 10.1016/j.scitotenv.2023.167068 -
Journal of Morphology Aug 2023Animal weapons are morphological traits that improve the fighting ability of the wielder and are associated with competition. These traits are typically sexually...
Animal weapons are morphological traits that improve the fighting ability of the wielder and are associated with competition. These traits are typically sexually dimorphic, with males possessing weaponry and females lacking weaponry. However, in some cases, like in many crustaceans, both males and females wield enlarged claws, which may function as weapons. Further, animal weapons may vary in their size, shape, and performance, with theory predicting that selection pressure for weaponry should be the highest when the importance of fights is the greatest, such as during a reproductive season. However, the degree and direction of selection may vary based on sex and season, with females potentially benefiting from wielding larger weapons during nonreproductive seasons. Crayfishes offer an ideal system to investigate how weapon phenotypes change across reproductive seasons since both males and females undergo a form alteration associated with reproduction. Thus, we investigated whether female Allegheny crayfish, Faxonius obscurus, claws change in size, shape, or pinching strength based on whether a female is in a reproductive or nonreproductive form. We found that female F. obscurus claws are larger and stronger during the reproductive season. These findings align with previous research on males of the same species. We discuss how predictions about the relationship between seasonality and weapon investment may differ based on sex.
Topics: Male; Female; Animals; Astacoidea; Seasons; Hoof and Claw; Reproduction
PubMed: 37458087
DOI: 10.1002/jmor.21614 -
Chemosphere Dec 2023The present study was aimed at evaluating the in vivo effects of microplastics (MP), in terms of oxidative stress and histopathological effects, in two crustacean...
The present study was aimed at evaluating the in vivo effects of microplastics (MP), in terms of oxidative stress and histopathological effects, in two crustacean species: Procambarus clarkii and Leptuca pugilator. In addition, MP accumulation in the hepatopancreas (HP) of both species was also determined. Adults of both crayfish and crabs were exposed for one month to fluorescent polystyrene beads (size: 1 μm) at nominal concentrations of 1000 or 5000 particles/mL. During the exposure, animals were maintained under controlled feeding, aeration, temperature, and photoperiod conditions. At the end of the exposure, HP and hemolymph (HL) samples were harvested for analysis of oxidative damage and total antioxidant levels. Additionally, the presence of MPs in both tissues was confirmed. Significant differences with the control groups were observed in lipid peroxidation levels in HP in animals exposed to the lowest concentration in P. clarkii and to the highest concentration in L. pugilator. A marked increase in antioxidant levels was also observed in the HL at both concentrations in P. clarkii, and at the highest MPs concentration in L. pugilator. Moreover, several histopathological changes were detected in both gills and HP, including hypertrophied lamellae, lifting or collapse of gill epithelia, loss of normal shape of hepatopancreatic tubules, and epithelial atrophy in the HP tissue. We conclude that exposure to MP beads at selected concentrations results in oxidative damage, induces histopathological changes in gills and HP, and triggers an antioxidant response in two crustacean species.
Topics: Animals; Astacoidea; Brachyura; Plastics; Antioxidants; Microplastics; Water Pollutants, Chemical; Oxidative Stress
PubMed: 37742760
DOI: 10.1016/j.chemosphere.2023.140260 -
Microbial Ecology Nov 2023Intestinal microbiota plays an important role in promoting digestion, metabolism, and immunity. Intestinal microbiota and fatty acids are important indicators to...
Intestinal microbiota plays an important role in promoting digestion, metabolism, and immunity. Intestinal microbiota and fatty acids are important indicators to evaluate the health and nutritional composition of Procambarus clarkii. They have been shown to be strongly influence by environmental and genetic factors. However, it is not clear whether environmental factors have a greater impact on the intestinal microbiota and fatty acid composition of crayfish. The link between the intestinal microbial communities and fatty acid (FA) compositions of red swamp crayfish from different geographical has not yet been studied. Thus, the current paper focuses on the influence of different environments on the fatty acids in muscles of crayfish and the possible existence between gut microbiota and fatty acids. Therefore, in this study, we compared the fatty acid compositions and intestinal microbiota of five crayfish populations from different geographical locations. The results were further analyzed to determine whether there is a relationship between geographical location, fatty acid compositions and intestinal microbiota. The gut microbial communities of the crayfish populations were characterized using 16S rRNA high-throughput gene sequencing. The results showed that there were significant differences in FA compositions of crayfish populations from different geographical locations. A similar trend was observed in the gut microbiome, which also varied significantly according to geographic location. Interestingly, the analysis revealed that there was a relationship between fatty acid compositions and intestinal microbes, revealed by alpha diversity analysis and cluster analysis. However, further studies of the interactions between the P. clarkii gut microbiota and biochemical composition are needed, which will ultimately reveal the complexity of microbial ecosystems with potential applications in aquaculture and species conservation.
Topics: Animals; Gastrointestinal Microbiome; Astacoidea; RNA, Ribosomal, 16S; Microbiota; Fatty Acids
PubMed: 37878052
DOI: 10.1007/s00248-023-02298-4 -
Food Chemistry May 2024The purpose of this study was to compare the influences of gamma-poly glutamic acid (γ-PGA) (1, 2, 3, and 4 %) to see which could outperform conventional...
The purpose of this study was to compare the influences of gamma-poly glutamic acid (γ-PGA) (1, 2, 3, and 4 %) to see which could outperform conventional cryoprotectant mixture (4 % sorbitol + 4 % sucrose) on cooked crayfish properties, such as physicochemical, textural qualities, oxidation reaction, water distributions, and microstructure integrity, during different freeze-thaw cycles. Crayfish quality characteristics improved significantly as γ-PGA concentration increased compared to control samples.Adding γ-PGA 4 % reduced the carbonyl content from 4.20 to 3.00 nmol/ mg protein during fluctuation-1 (F1), and from 4.15 to 2.80 nmol/ mg protein during fluctuation-2 (F2) compared to control samples. Furthermore, it increased the total sulfhydryl content from 4.15 and 4.76 to 6.19 and 6.47 mol/10 g protein during F1 and F2 and after five freeze-thaw cycles (FTC). This suggests that this concentration was more effective at controlling protein changes than other concentrations. γ-PGA generally enhanced the water-holding capacity by preventing protein denaturation and limiting ice crystal recrystallization. As a result, microstructure stability was evident, texture degradation was avoided, and the crayfish's color was preserved.
Topics: Animals; Astacoidea; Temperature; Freezing; Water; Polyglutamic Acid
PubMed: 38219359
DOI: 10.1016/j.foodchem.2023.138258 -
Ultrasound in Medicine & Biology Dec 2023Focused ultrasound (FUS) can modulate neuronal activity by depolarization or hyperpolarization. Although FUS-evoked depolarization has been studied extensively, the...
OBJECTIVE
Focused ultrasound (FUS) can modulate neuronal activity by depolarization or hyperpolarization. Although FUS-evoked depolarization has been studied extensively, the mechanisms underlying FUS-evoked hyperpolarization (FUSH) have received little attention. In the study described here, we developed a procedure using FUS to selectively hyperpolarize motor axons in crayfish. As a previous study had reported that these axons express mechano- and thermosensitive two-pore domain potassium (K2P) channels, we tested the hypothesis that K2P channels underlie FUSH.
METHODS
Intracellular recordings from a motor axon and a muscle fiber were obtained simultaneously from the crayfish opener neuromuscular preparation. FUSH was examined while K2P channel activities were modulated by varying temperature or by K2P channel blockers.
RESULTS
FUSH in the axons did not exhibit a coherent temperature dependence, consistent with predicted K2P channel behavior, although changes in the resting membrane potential of the same axons indicated well-behaved K2P channel temperature dependence. The same conclusion was supported by pharmacological data; namely, FUSH was not suppressed by K2P channel blockers. Comparison between the FUS-evoked responses recorded in motor axons and muscle fibers revealed that the latter exhibited very little FUSH, indicating that the FUSH was specific to the axons.
CONCLUSION
It is not likely that K2P channels are the underlying mechanism for FUSH in motor axons. Alternative mechanisms such as sonophore and axon-specific potassium channels were considered. Although the sonophore hypothesis could account for electrophysiological features of axonal recordings, it is not consistent with the lack of FUSH in muscle fibers. An axon-specific and mechanosensitive potassium channel is also a possible explanation.
Topics: Animals; Astacoidea; Axons; Neuromuscular Junction; Neurons; Potassium Channels; Muscle Fibers, Skeletal
PubMed: 37758529
DOI: 10.1016/j.ultrasmedbio.2023.08.016 -
The British Journal of Nutrition Sep 2023For the omnivorous crayfish, plant raw materials can be good alternatives to dietary fish meal (FM). A 56-d feeding trial was conducted in (11·70 (se 0·13) g). Diet...
Effects of dietary plant protein sources intake on growth, digestive enzyme activity, edible tissue nutritional status and intestinal health of the omnivorous Redclaw crayfish, .
For the omnivorous crayfish, plant raw materials can be good alternatives to dietary fish meal (FM). A 56-d feeding trial was conducted in (11·70 (se 0·13) g). Diet with 100 % FM as the protein source was the control. Seven experimental diets were formulated by replacing 75 or 100 % of FM with soyabean meal (SM75, SM100) or cottonseed meal (CM75 and CM100), and a mixture of SM and CM (protein content is 1:1) replacing 50, 75 or 100 % of FM (SC50, SC75 and SC100). Crayfish fed the CM100 and SC100 showed significantly lower weight gain (WG), specific growth rate, trypsin and pepsin activities compared with the control diet. Crayfish in CM100 group showed significantly higher GPx, alanine aminotransferase, aspartate aminotransferase activities and malondialdehyde content than the control. SM100 and CM100 diets can cause slight separation of the peritrophic membrane from the intestinal folds. The pepsin activity of crayfish in SC50 was significantly higher than those in other experimental diets. The highest WG and muscle arginine content were also found in crayfish fed SC50. The relative abundance of , and was significantly higher, but was significantly lower in SM100, CM100 and SC100 than in control. Microbiota functional prediction indicated that the relative abundance of 'cell motility' pathway in crayfish fed CM100 was significantly decreased compared with the control. In conclusion, only half of the FM can be effectively substituted with a mixture of SM and CM (protein content is 1:1) for .
Topics: Animals; Astacoidea; Nutritional Status; Plant Proteins, Dietary; Pepsin A; Intestines; Diet; Animal Feed
PubMed: 36597816
DOI: 10.1017/S0007114522004044 -
PloS One 2024Burrowing is a common trait among crayfish thought to help species deal with adverse environmental challenges. However, little is known about the microhabitat ecology of...
Burrowing is a common trait among crayfish thought to help species deal with adverse environmental challenges. However, little is known about the microhabitat ecology of crayfish taxa in relation to their burrows. To fill this knowledge gap, we assessed the availability of oxygen inside the crayfish shelter by series of in-vivo and in-silico modelling experiments. Under modeled condition, we found that, except for the entrance region of the 200 mm, a flooded burrow microenvironment became anoxic within 8 h, on average. Multiple 12-hour day-night cycles, with burrows occupied by crayfish for 12 h and empty for 12 h, were not sufficient for refreshing the burrow microenvironment. We then examined the degree to which crayfish species with different propensities for burrowing are tolerant of self-created anoxia. From these experiments, primary and secondary burrowers showed best and most consistent tolerance-exhibiting ≥ 64% survival to anoxia and 25-91% survival of ≥ 9 h at anoxia, respectively. Tertiary burrowers exhibited little to no tolerance of anoxia with 0-50% survival to anoxia and only one species exhibiting survival (2%) of ≥ 9 h at anoxia. Results suggest that moderate to strongly burrowing crayfish can quickly draw down the dissolved oxygen in burrow water but appear to have conserved a legacy of strong tolerance of anoxia from their monophyletic ancestors-the lobsters-whereas tertiary burrowers have lost (or never evolved) this ability.
Topics: Animals; Astacoidea; Decapoda; Water; Hypoxia; Ecology; Oxygen
PubMed: 38165988
DOI: 10.1371/journal.pone.0287888 -
Comparative Biochemistry and... Apr 2024Iron is a common and essential element for maintaining life in bacteria, plants and animals and is found in soil, fresh waters and marine waters; however, over exposure...
Iron is a common and essential element for maintaining life in bacteria, plants and animals and is found in soil, fresh waters and marine waters; however, over exposure is toxic to organisms. Iron is used in electron transport complexes within mitochondria as well as a co-factor in many essential proteins. It is also established that iron accumulation in the central nervous system in mammals is associated with various neurological disorders. Ample studies have investigated the long-term effects of iron overload in the nervous system. However, its acute effects in nervous tissue and additional organ systems warrant further studies. This study investigates the effects of iron overload on development, behavior, survival, cardiac function, and glutamatergic synaptic transmission in the Drosophila melanogaster. Additionally, physiological responses in crayfish were examined following Fe exposure. Fe reduced neuronal excitability in proprioceptive neurons in a crayfish model. Thus, Fe may block stretch activated channels (SACs) as well as voltage-gated Na channels. Exposure also rapidly reduces synaptic transmission but does not block ionotropic glutamatergic receptors, suggesting a blockage of pre-synaptic voltage-gated Ca channels in both crustacean and Drosophila models. The effects are partly reversible with acute exposure, indicating the cells are not rapidly damaged. This study is relevant in demonstrating the effects of Fe on various physiological functions in different organisms in order to further understand the acute and long-term consequences of overload.
Topics: Animals; Iron; Drosophila melanogaster; Iron Overload; Physiological Phenomena; Astacoidea; Invertebrates; Mammals
PubMed: 38354992
DOI: 10.1016/j.cbpc.2024.109856 -
Journal of the Science of Food and... Jan 2024Peeling of crayfish is a very important process in production. Crayfish peeling by machine can increase production efficiency and enhance safety in the production...
BACKGROUND
Peeling of crayfish is a very important process in production. Crayfish peeling by machine can increase production efficiency and enhance safety in the production process. The tight muscle-shell attachment causes difficulty in peeling freshly caught crayfish. However, few studies have explored the changes in crayfish quality under favorable shell-loosening treatments.
RESULTS
In this study, the shell-loosening properties of crayfish and changes in crayfish quality, microstructure and protein fluorescent features were investigated after high hydrostatic pressure (HHP) treatment. New methods were established to measure the peeling performance of crayfish, which are peelability and meat yield rate (MYR). The normalization of peelability and MYR were verified by different weights of crayfish tails and different treatments. The peeling effect of HHP-treated crayfish was evaluated by a new quantitative measurement method, and MYR was calculated. The results showed that all the HHP treatments reduced crayfish peeling work and increased MYR. The HHP treatment provided better crayfish quality in terms of texture and color and enlarged the shell-loosening gap. Among all HHP treatments, 200 MPa treatment exhibited lower peeling work, higher MYR and an expansion of the shell-loosening gap, reaching up to 573.8 μm. At the same time, 200 MPa treatment could maintain crayfish quality.
CONCLUSION
The findings outlined above suggest that high pressure is a promising method for loosening crayfish shells. 200 MPa is an optimal HHP treatment condition for crayfish peeling, exhibiting a promising application in industrial processing. © 2023 Society of Chemical Industry.
Topics: Animals; Hydrostatic Pressure; Astacoidea; Food Microbiology; Food Handling; Seafood
PubMed: 37437092
DOI: 10.1002/jsfa.12855