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Journal of Veterinary Diagnostic... Nov 2023The Western honey bee () is economically important as the primary managed pollinator of many agricultural crops and for the production of various hive-related...
The Western honey bee () is economically important as the primary managed pollinator of many agricultural crops and for the production of various hive-related commodities. Honey bees are not classically or thoroughly covered in veterinary pathology training programs. Given their unique anatomic and biological differences from the other species more traditionally evaluated by veterinary pathologists, establishing routine and consistent methods for processing samples for histology ensures accurate diagnostic and research conclusions. We developed and tested several field protocols for the sampling of honey bees. We compared the tissue-quality outcomes for worker bees fixed, collected, and/or softened under the following protocols: 1) routine formalin fixation; 2) softening chitin via exposure to Nair for 2 d or 3) 5 d; 4) shortened times between formalin submersion and trimming of body segments to enhance penetration of formalin into internal tissues; 5) ethanol submersion of specimen prior to formalin fixation; 6) indirect dry ice exposure; and 7) prolonged -80°C storage. Routine formalin fixation, exposure to Nair for 2 d, indirect dry ice exposure, and trimming body segments within 2 h of formalin submersion resulted in the highest quality histologic tissue sections. The poorest quality sections resulted from softening of chitin by exposure to Nair for 5 d, submersion in ethanol for 3 d before formalin fixation, and prolonged storage at -80°C. Our results indicate that routine formalin fixation is adequate, and that immobilizing bees with indirect dry ice exposure aids in sample collection without negatively impacting the quality of histologic sections.
Topics: Bees; Animals; Dry Ice; Formaldehyde; Chitin; Ethanol
PubMed: 37587755
DOI: 10.1177/10406387231191732 -
Advanced Science (Weinheim,... Aug 2023Bioelectric electrodes with low modulus and high adhesion have been intensively pursued, as they afford conformal and strong bonding at skin-electrode interface to...
Bioelectric electrodes with low modulus and high adhesion have been intensively pursued, as they afford conformal and strong bonding at skin-electrode interface to improve the fidelity and stability of electrophysiological signals. However, during detachment, tough adhesion can cause pain or skin allergy; worse still, the soft electrodes can suffer damage due to excessive stretch/torsion, hampering long-term, dynamic, and multiple uses. Herein, a bioelectric electrode is proposed by transferring silver nanowires (AgNWs) network to the surface of bistable adhesive polymer (BAP). The phase transition temperature of BAP is tuned to be slightly below skin temperature at 30 °C. Triggered by skin heat, the BAP electrode achieves low modulus and high adhesion within seconds, allowing robust skin-electrode interface under dry, wet, and body-moving conditions. Ice bag treatment can dramatically stiffen the electrode and reduce the adhesion, which allows painless detachment and avoids electrode damage. Meanwhile, the AgNWs network with biaxial wrinkled microstructure remarkably promotes the electro-mechanical stability of the BAP electrode. The BAP electrode successfully combines long-term (7 days) and dynamic (body movements, sweat, underwater) stability, reusability (at least ten times), and minimized skin irritation during electrophysiological monitoring. The high signal-to-noise ratio and dynamic stability are demonstrated in the application of piano-playing training.
PubMed: 37199692
DOI: 10.1002/advs.202300793 -
Foods (Basel, Switzerland) Jul 2023In this paper, the quality change of Yesso scallop () in the process of anhydrous storage and transportation after cold acclimation and induced dormancy was studied, and...
In this paper, the quality change of Yesso scallop () in the process of anhydrous storage and transportation after cold acclimation and induced dormancy was studied, and the regulation mechanism of quality degradation during storage and transportation in the process of gradient chilling stress and drying exposure was further explored. The results show that, when transferred from hydrous to anhydrous states, the breathing pattern of the scallops changed from aerobic to anaerobic. Their gill filaments were altered and their apparent vitality constantly declined, which was reflected by the edge shrinkage of the pallium and the direct proportions of the edge reduction rate and the stimulus response period. After being in the anhydrous state for 4 d, the AEC value dropped to 67.59%. At this time, if they were placed under hydration again, the scallops resumed a good growth state. By proteomics analysis, it was revealed that cold acclimation and dry exposure mainly led to changes in biological functions and pathways, such as mitochondrial inner membrane and ATP hydrolysis activity. In addition, it can be seen from the functional annotation and enrichment analysis of the metabolite KEGG that cold acclimation promoted the purine metabolism of scallops, while dry exposure inhibited the metabolism of saturated fatty acids. In this study, the infrared sensing mode was used for the first time, too, in order to record the heart-rate changes of the scallops during circulation, which shows that non-destructive vitality monitoring of is feasible.
PubMed: 37569171
DOI: 10.3390/foods12152902 -
Journal of Food Science and Technology May 2024"Aquafaba", defined as legume cooking water, has a feature that can be used in various formulations as an egg and milk alternative in vegan products and improves...
UNLABELLED
"Aquafaba", defined as legume cooking water, has a feature that can be used in various formulations as an egg and milk alternative in vegan products and improves functional properties such as foaming, emulsifying and gelling. In this study, it was aimed to investigate the usability of aquafaba in ice cream type frozen desserts containing different fruit purees (strawberry, nectarine and banana) by using its foaming capacity. Rheological properties, microstructure, overrun, melting characteristics, color measurement, dry matter, and sensory properties were investigated in different fruit-based frozen dessert samples. The brix value, density, protein content, foaming capacity (FC) and foaming stability (FS), flow behavior index, consistency coefficient, and overrun of aquafaba were determined as 7.1 ± 0°Bx, 1.022 ± 0.011 g/ml, 1.51 ± 0.41%, 85 ± 0% FC and 81 ± 0.23% FS, between 0.28 and 0.64, between 8.68 and 41.30 Pa·sn, between 116.75 and 395.93%, respectively. The dry matter content of the strawberry, nectarine, and banana-based dessert samples ranged between 17 and 48%, 20-49%, 25-50%, and the first dropping times were determined between 348 and 1538 s, 369-1689 s and 435-1985 s, respectively. As a result, cooking liquid leftover aquafaba can be used as a suitable raw material in the production of an alternative ice cream type frozen dessert for individuals with milk allergy, lactose intolerance or who prefer a vegan diet.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s13197-023-05885-y.
PubMed: 38487276
DOI: 10.1007/s13197-023-05885-y -
Chemosphere Nov 2023Relics of World War One (WW1) were buried in alpine glaciers around 100 years ago. Today, these are emerging from the ice due to widespread glacier retreat, and are in...
Relics of World War One (WW1) were buried in alpine glaciers around 100 years ago. Today, these are emerging from the ice due to widespread glacier retreat, and are in direct contact with glacial meltwater-fed streams. To address a possible emergent contamination, we quantified major and trace elements (M-TEs) by mass spectrometry in water and larvae of Diamesa zernyi from three glacial streams fed by glaciers differently impacted by the Italian Austro-Hungarian war, in the Adamello-Presanella mountain range (Italian Alps): Lares and Presena, the two main battlefields, and Amola, 8 km from the front. M-TEs in stream water were interpreted using the crustal enrichment factor (EFc) while larval uptake was quantified by adopting the bioaccumulation factor (BAF). Despite low M-TEs concentrations in the water, in a range between 1 ng L (Ag, Ta) and 1-2 mg L (Al, Fe, Mg), low to moderate enrichments (10 ≥ EFc≥ 6) were observed for Sb and U in Presena and for Ag, As, Bi, Cd, Li, Mo, Pb, Sb and U in Lares. In addition, M-TE mass concentrations in larvae were up to ninety thousand times higher than in water, from 20 to 50 ng g dry weight (d.w.; for Bi, Sb, Ta, Tl) to 1-4 mg g d.w. (for Al, Fe, Na, and Mg). Larvae from Lares accumulated the largest amount of metals and metalloids, including those mostly used in the manufacture of artillery shells (As, Cu, Ni, Pb, Sb; BAFs from 375 to about 11,500). This was expected as most of the WW1 battles in this mountain range were fought on the Lares glacier, where the greatest number of war relics are emerging. These results provide preliminary evidence of water contamination and bioaccumulation of metals and metalloids by glacial fauna as a possible legacy of WW1 in the Alps.
Topics: Animals; Water; Chironomidae; Lead; Environmental Monitoring; Ice Cover; Italy; Trace Elements
PubMed: 37574091
DOI: 10.1016/j.chemosphere.2023.139757 -
Journal of Safety Research Dec 2023In parts of Europe and North America, cycling volumes decrease in winter due to a reduction in subjective safety. To counter this, high-quality winter maintenance is...
INTRODUCTION
In parts of Europe and North America, cycling volumes decrease in winter due to a reduction in subjective safety. To counter this, high-quality winter maintenance is required on cycle paths. At the moment, grit and sodium chloride are considered state-of-the-art gritting/de-icing materials in Germany. However, grit has to be removed after winter because it poses a serious injury risk on dry streets, and, in various German cities, using sodium chloride is prohibited on segregated bike paths due to the harmful impact on surrounding trees. Therefore, there is a need for alternative gritting/de-icing materials.
METHOD
We used a mixed-methods approach consisting of qualitative and quantitative surveys together with laboratory investigations and a life cycle assessment to find suitable alternatives to sodium chloride and grit for use on segregated bike paths, and tested four de-icing materials (sodium chloride as a reference, sodium formate, calcium magnesium acetate or CMA, and potassium acetate) at two sites in Hamburg, Germany. The tests were accompanied by on-site cyclist surveys.
RESULTS
The results show that the use of alternative (non-sodium chloride) de-icing materials either reduces or eliminates negative impacts on the environment at a local level, for example on trees along the cycle path. However, this reduction goes hand in hand with increasing negative environmental impacts at a global level due to higher overall emissions associated with the tested alternative de-icing materials. Regarding cyclists' safety, sodium formate was the only de-icing material which delivered comparable results to sodium chloride and should therefore be tested in extended conditions.
CONCLUSIONS
Further research is needed on the large-scale application of the investigated de-icing agents on cycle paths in different cities, along with a survey of a larger number of cyclists. A final evaluation of the environmental aspects can only be made when the supply and production conditions for large-scale use are clear.
PRACTICAL APPLICATIONS
Basically there are two options for the practical application of de-icing materials: The usage of the costlier alternatives with better properties at a local level in the hope of fast development towards a better global GHG footprint in their manufacturing processes, or sticking to the use of grit and sodium chloride (where it is possible) while committing to improving clean-up after the snow and ice melt to prevent unsafe road conditions in spring.
Topics: Humans; Sodium Chloride; Cities; Bicycling; Formates
PubMed: 38081698
DOI: 10.1016/j.jsr.2023.09.020 -
Integrated Environmental Assessment and... Mar 2024Lemna L. sp. is a free-floating aquatic macrophyte that plays a key role as a standard test species in aquatic risk assessment for herbicides and other contaminants....
Lemna L. sp. is a free-floating aquatic macrophyte that plays a key role as a standard test species in aquatic risk assessment for herbicides and other contaminants. Population modeling can be used to extrapolate from laboratory to field conditions. However, there are insufficient data on longer-term seasonal dynamics of this species to evaluate such models. Therefore, several long-term growth experiments were conducted in outdoor microcosms (surface area 0.174 m). Monitoring parameters included biomass, frond numbers, water parameters, and weather data. Three different datasets were generated: frond numbers and biomass from weekly to monthly destructively sampled microcosms; a year-round dataset of frond numbers from five continuously monitored microcosms; and seasonal growth rates without the effect of density dependence over 1-2 weeks in freshly inoculated microcosms. Lemna sp. reached a maximum of approximately 500 000 fronds m and 190 g dry weight m. During the first winter, the microcosms were covered by ice for approximately four weeks, and Lemna sp. populations collapsed. The second winter was warmer, without any ice cover, and Lemna sp. populations maintained high abundance throughout the winter. Dry weight per frond was not constant throughout the year but was highest in autumn and winter. Growth rates without density dependence under outdoor environmental conditions reached 0.29 day for frond number, 0.43 day for fresh weight, and 0.39 day for dry weight. In linear regressions, these growth rates were best explained by water temperature. For the populations continuously monitored throughout a year, the nitrogen-to-phosphorus ratio best explained the growth rate of frond numbers. This study yielded a relevant dataset for testing and refining Lemna population models used in chemical risk assessment as well as for managing ecosystems and combating the effects of eutrophication. Integr Environ Assess Manag 2024;00:1-14. © 2024 The Authors. Integrated Environmental Assessment and Management published by Wiley Periodicals LLC on behalf of Society of Environmental Toxicology & Chemistry (SETAC).
PubMed: 38546104
DOI: 10.1002/ieam.4916 -
Sensors (Basel, Switzerland) Mar 2024This work presents an approach that integrates the results of measuring, analyzing, and modeling air flow phenomena driven by pressurized air in a flue organ pipe. The...
This work presents an approach that integrates the results of measuring, analyzing, and modeling air flow phenomena driven by pressurized air in a flue organ pipe. The investigation concerns a Bourdon organ pipe. Measurements are performed in an anechoic chamber using the Cartesian robot equipped with a 3D acoustic vector sensor (AVS) that acquires both acoustic pressure and air particle velocity. Also, a high-speed camera is employed to observe the jet coming out from the windway. For that purpose, the steam resulting from dry ice and hot water is used. A numerical simulation of the sounding mechanism of a pipe of the same geometry is based on measuring the pressure signal and the intensity field around the mouth employing an intensity probe and visualizing and observing the motion of the air jet, which represents the excitation mechanism of the system. The ParaVIEW software serves for the simulation and visualization of the air jet. Then, the results obtained from measurements and simulations are compared and discussed. Also, some future directions discussing the application of a machine-learning approach to the area of pipe organ air flow investigation are contained in the Conclusions section.
PubMed: 38544227
DOI: 10.3390/s24061962 -
Acta Medica Okayama Feb 2024Ice bags are frequently used in medical care settings for pain relief, comfort, and in some cases, whole-body cooling. This study quantifies heat energy transfer...
Ice bags are frequently used in medical care settings for pain relief, comfort, and in some cases, whole-body cooling. This study quantifies heat energy transfer capacity of ice bags and evaluates their cooling effects on body temperature. Forty-eight healthy adults in their 20s were recruited. An ice bag wrapped in two layers of dry towel was applied to the forehead, neck, or palm of each participant for 10 min. The skin surface temperature, heat flow, and core temperature were recorded during the cooling and non-cooling periods, with energy transfer calculated by integrating heat flow over time. Over the non-cooling period, 31.4-53.6 kJ·m-2 of energy was dissipated over 10 min, whereas during the cooling period, the range increased to 180.0-218.7 kJ·m-2 over 10 min. Skin surface temperature decreased by 3.2-5.7°C, whereas core temperature was unchanged. Ice bag use augmented energy transfer by about 150-180 kJ·m-2 over 10 min, but this was insufficient for rapid whole body cooling due to the small skin-surface area in contact with the ice bag. The measured energy transfer indicated that topical ice bag application absorbs insufficient energy to affect core temperature. Quantitative assessment of energy transfer was shown to inform the safe and appropriate use of thermotherapy.
Topics: Adult; Humans; Hot Temperature; Temperature; Cold Temperature
PubMed: 38419315
DOI: 10.18926/AMO/66671 -
Research Square Dec 2023Lipids play many important physiological roles in mammalian reproduction, being essential for the acquisition of oocyte competence and post-fertilization embryonic...
Lipids play many important physiological roles in mammalian reproduction, being essential for the acquisition of oocyte competence and post-fertilization embryonic development. Lipid profiling in samples of minute size, such as oocytes, is challenging but has been achieved by mass spectrometry technologies such as multiple reaction monitoring (MRM) profiling. With the goals of further simplifying sample workflow and investigating the influence of pre-analytical conditions, we have evaluated how different extraction methods and transportation of lipid extracts in vacuum and at room temperature impacted the lipid profile of bovine oocytes. Using a comprehensive method, 316 MRMs associated with lipids of 10 different classes were screened in oocyte lipid extracts prepared by 2 extraction methods (one-step methanol addition or Bligh and Dyer) and transporting them in dry ice or at room temperature inside vacuum packages. No changes in the multivariate analysis (PCA) were noticeable due to transportation temperature, while lipid profiles were more affected by the lipid extraction protocol. Sample extraction using pure methanol favored the detection of phospholipids uniformly, while Bligh and Dyer favored the detection of neutral intracellular lipids. Triacylglycerol lipids and free fatty acids yielded decreased abundances when samples were transported at room temperature. We conclude that if samples are submitted to the same lipid extraction protocol and same transportation batch at room temperature coupled with vacuum conditions it is possible to analyze lipid extracts of bovine oocytes and still obtain informative lipid profiling results.
PubMed: 38196623
DOI: 10.21203/rs.3.rs-3788683/v1