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Microbial Cell Factories Dec 2023Color chemicals contaminate pure water constantly discharged from different points and non-point sources. Physical and chemical techniques have certain limitations and...
Color chemicals contaminate pure water constantly discharged from different points and non-point sources. Physical and chemical techniques have certain limitations and complexities for bioenergy production, which motivated the search for a novel sustainable production approaches during dye wastewater treatment. The emerging environmental problem of dye decolorization has attracted scientist's attention to a new, cheap, and economical way to treat dye wastewater and power production via fungal fuel cells. Ganoderma gibbosum was fitted in the cathodic region with laccase secretion in the fuel cell. At the same time, dye water was placed in the anodic region to move electrons and produce power. This study treated wastewater using the oxidoreductase enzymes released extracellularly from Ganoderma gibbosum for dye Remazol Brilliant Blue R (RBBR) degradation via fungal-based fuel cell. The maximum power density of 14.18 mW/m and the maximum current density of 35 mA/m were shown by the concentration of 5 ppm during maximum laccase activity and decolorization of RBBR. The laccase catalysts have gained considerable attention because of eco-friendly and alternative easy handling approaches to chemical methods. Fungal Fuel Cells (FFCs) are efficiently used in dye treatment and electricity production. This article also highlighted the construction of fungal catalytic cells and the enzymatic performance of fungal species in energy production during dye water treatment.
Topics: Laccase; Wastewater; Coloring Agents; Electricity
PubMed: 38098010
DOI: 10.1186/s12934-023-02258-0 -
Biotechnic & Histochemistry : Official... Nov 2024Romanowsky staining was an important methodological breakthrough in diagnostic hematology and cytopathology during the late 19 and early 20 centuries; it has facilitated... (Review)
Review
Romanowsky staining was an important methodological breakthrough in diagnostic hematology and cytopathology during the late 19 and early 20 centuries; it has facilitated for decades the work of biologists, hematologists and pathologists working with blood cells. Despite more than a century of studying Romanowsky staining, no systematic review has been published that explains the chemical processes that produce the "Romanowsky effect" or "Romanowsky-Giemsa effect" (RGE), i.e., a purple coloration arising from the interaction of an azure dye with eosin and not due merely to their simultaneous presence. Our review is an attempt to build a bridge between chemists and biomedical scientists and to summarize the available data on methylene blue (MB) demethylation as well as the related reduction and decomposition of MB to simpler compounds by both light and enzyme systems and microorganisms. To do this, we analyze modern data on the mechanisms of MB demethylation both in the presence of acids and bases and by disproportionation due to the action of light. We also offer an explanation for why the RGE occurs only when azure B, or to a lesser extent, azure A is present by applying experimental and calculated physicochemical parameters including dye-DNA binding constants and electron density distributions in the molecules of these ligands. Finally, we discuss modern techniques for obtaining new varieties of Romanowsky dyes by modifying previously known ones. We hope that our critical literature study will help scientists understand better the chemical and physicochemical processes and mechanisms of cell staining with such dyes.
Topics: Azure Stains; Staining and Labeling; Coloring Agents; Methylene Blue; Eosine Yellowish-(YS)
PubMed: 37929609
DOI: 10.1080/10520295.2023.2273860 -
ACS Nano Jan 2024We present super-resolution microscopy of isolated functional mitochondria, enabling real-time studies of structure and function (voltages) in response to...
We present super-resolution microscopy of isolated functional mitochondria, enabling real-time studies of structure and function (voltages) in response to pharmacological manipulation. Changes in mitochondrial membrane potential as a function of time and position can be imaged in different metabolic states (not possible in whole cells), created by the addition of substrates and inhibitors of the electron transport chain, enabled by the isolation of vital mitochondria. By careful analysis of structure dyes and voltage dyes (lipophilic cations), we demonstrate that most of the fluorescent signal seen from voltage dyes is due to membrane bound dyes, and develop a model for the membrane potential dependence of the fluorescence contrast for the case of super-resolution imaging, and how it relates to membrane potential. This permits direct analysis of mitochondrial structure and function (voltage) of isolated, individual mitochondria as well as submitochondrial structures in the functional, intact state, a major advance in super-resolution studies of living organelles.
Topics: Mitochondria; Organelles; Microscopy; Membrane Potentials; Coloring Agents; Fluorescent Dyes
PubMed: 37289571
DOI: 10.1021/acsnano.3c02768 -
Water Environment Research : a Research... Dec 2023A notable level of apprehension exists over the adverse impacts of dye pollution on aquatic ecosystems and human well-being. The primary objective of this research is to...
A notable level of apprehension exists over the adverse impacts of dye pollution on aquatic ecosystems and human well-being. The primary objective of this research is to assess the effectiveness of Fenton catalytic reactions in degrading 14 different commercial azo dyes (both single and double) present in aqueous solutions. The investigation focused on the function of dye structures, using a combination of experimental data and examination of theoretical factors. Dye degradation process was carried out at pH 3, and the concentrations of Fe (10 mol/L), H O (2 × 10 mol/L), and dye (0.05 g/L). The findings revealed that dyes with a larger molecular weight were more effective at degrading (D%), with the overall degradation efficiency varying from 0% to 94%. Functional groups played an important role in degradation efficiency; for example, dyes with higher aromatic rings led to less D%, while a higher number of sulfonic, methyl, and nitro groups was responsible for better D%. Notably, the presence of OH groups in the backbone of dyes (AB 24, ABE 113, and MB 9) formed the Fe complex during the catalytic process, and the D% was minimal. On the other hand, theoretical quantum calculations such as the greater the JCLogP, highest occupied molecular orbital, and Dipole moment value, the higher the degradation efficiency. And dyes with low lowest unoccupied molecular orbital tended to have a better degradation efficiency. To some extent, UV-Vis spectral analysis was investigated to determine the degradation pathway, and the pseudo-second-order kinetic model fitted better in the degradation process. The overall experimental and theoretical findings suggested that dye degradation efficiency by the Fenton process is structure-dependent. PRACTITIONER POINTS: Insights into the role of azo dye structures-properties on degradation efficiency. Higher molecular weight and sulfonic groups containing dyes showed better degradation efficiency. Hydroxyl groups play the formation of the Fe complex during the degradation process. Higher values of HOMO and lower values of LUMO enhanced degradation efficiency. The pseudo-second-order (PSO) kinetic model obeyed the Fenton process.
Topics: Humans; Coloring Agents; Wastewater; Iron; Ecosystem; Hydrogen Peroxide; Azo Compounds
PubMed: 38062884
DOI: 10.1002/wer.10948 -
International Journal of Biological... Dec 2023Chitosan (Ch)-diatomite (D) composite beads (Ch-D) were synthesized using epichlorohydrin and tripolyphosphate crosslinkers and then moulded into uniform beads in...
Chitosan (Ch)-diatomite (D) composite beads (Ch-D) were synthesized using epichlorohydrin and tripolyphosphate crosslinkers and then moulded into uniform beads in tripolyphosphate solution. FT-IR and SEM-EDX analyses showed that Ch-D composite adsorbent was successfully synthesized by functionalization of chitosan to Ch-D by hydrogen bonding and electrostatic interactions and improved adsorption capacity for removal of Allura red AC (AR) food dye. The removal rate of AR food dye was found to be 98 % at 25 °C, natural AR dye pH value, 24 h, and at the initial AR dye concentration of 200 mg L. The maximum adsorption capacity was found to be 34.7 mg g. It was observed that π-π interactions, hydrogen bonds, and electrostatic interactions between the functional groups containing oxygen and amine on the surface of the Ch-D composite and the functional groups of the anionic AR dye, and AR dye adsorption via complexation took place. In light of all this information, the Ch-D composite adsorbent is a promising potential material for the effective treatment of colored pollutants from wastewater.
Topics: Chitosan; Water; Spectroscopy, Fourier Transform Infrared; Coloring Agents; Adsorption; Water Pollutants, Chemical; Hydrogen-Ion Concentration; Kinetics
PubMed: 37657566
DOI: 10.1016/j.ijbiomac.2023.126632 -
Environmental Science and Pollution... Aug 2023Water pollution caused by organic dyes is one of the greatest threats to the ecosystem. The removal of dyes from water has remained a challenge for... (Review)
Review
Water pollution caused by organic dyes is one of the greatest threats to the ecosystem. The removal of dyes from water has remained a challenge for scientists. Recently, metal sulphides have emerged as a potential candidate for water remediation applications. The efficient charge transportation, greater surface-active sites, and low bandgap of metal sulphides make them an excellent choice of semiconductor photocatalysts for degradation of dyes. This review summarises the potential application of metal sulphides and their heterojunctions for the photocatalytic degradation of organic dyes from wastewater. A detailed study has been presented on the synthesis, basics of photodegradation and heterojunctions and photocatalytic activity. The effect of the use of templates, doping agents, synthesis route, and various other factors affecting the photocatalytic activity of metal sulphides have been summarised in this review. The synthesis techniques, characterisation techniques, mechanism of degradation of organic dyes by Z-scheme heterojunction photocatalyst, reusability and stability of metal sulphides, and the scope of future research are also discussed. This study indicates that Scopus-based core gathered data could be used to give an objective overview of the global dye degradation research from 2008 to 2023 (15 years). All data (articles, authors, keywords, and publications) is compiled in the Scopus database. For the bibliometric study, 1962 papers relevant to dye photodegradation by sulfide-based photocatalysts were found, and this number rises yearly. A bibliometric analysis provides a 15-year evaluation of the state-of-the-art research on the impact of metal sulfide-based photocatalysts on the photodegradation of dyes.
Topics: Ecosystem; Catalysis; Metals; Water; Coloring Agents
PubMed: 37474851
DOI: 10.1007/s11356-023-28753-w -
Chemosphere Aug 2023It would be extremely momentous to familiarize a low-cost sole adsorbent NiAlFe-layered triple hydroxides (LTHs) having a strong sorption affinity towards both anionic...
It would be extremely momentous to familiarize a low-cost sole adsorbent NiAlFe-layered triple hydroxides (LTHs) having a strong sorption affinity towards both anionic and cationic dyes. Using the urea hydrolysis hydrothermal method LTHs were fabricated and by altering the ratio of participant metal cations the adsorbent was optimized. BET analysis revealed that the optimized LTHs possess an elevated surface area (160.04 m/g) while TEM and FESEM analysis portrayed the stacked sheets-like 2D morphology. LTHs were employed for the amputation of anionic congo red (CR) and cationic brilliant green (BG) dye. The adsorption study showed that within 20 and 60 min, respectively, maximum adsorption capacities were achieved at 57.47 mg/g and 192.30 mg/g for CR and BG dye. Adsorption isotherm, kinetics, and thermodynamics study revealed that both chemisorptions with physisorptions were the assertive factor for the dye encapsulation. This enhanced adsorption performance of the optimized LTH for the anionic dye is attributed to its inherent anions exchange properties and new bond formation with the adsorbent skeleton. Whereas for the cationic dye, it was because of the formation of strong hydrogen bonds, and electrostatic interaction. Morphological manipulation of LTHs, formulates the optimized adsorbent LTH111, provokes the adsorbent for this elevated adsorption performance. Overall, this study revealed that LTHs have a high potential for the effectual remediation of dyes from wastewater as a sole adsorbent at a low cost.
Topics: Humans; Coloring Agents; Hydroxides; Congo Red; Cations; Adsorption; Water Pollutants, Chemical; Kinetics
PubMed: 37172625
DOI: 10.1016/j.chemosphere.2023.138878 -
Environmental Research Apr 2024The excessive and uncontrollable discharge of diverse organic pollutants into the environment has emerged as a significant concern, presenting a substantial risk to...
The excessive and uncontrollable discharge of diverse organic pollutants into the environment has emerged as a significant concern, presenting a substantial risk to human health. Among the advanced oxidation processes used for the purification of wastewater, cold plasma technology is superior in fast and effective decontamination but often fails facing mixed pollutants. To address these issues, here we develop the new conceptual approach, plasma process, and proprietary reactor that ensure, for the first time, that the efficiency of treatment (114.7%) of two mixed organic dyes, methylene blue (MB) and methyl orange (MO), is higher than when the two dyes are treated separately. We further reveal the underlying mechanisms for the energy-efficient complete degradation of the mixed dyes. The contribution of plasma-induced ROS and the distinct degradation characteristics and mechanism of pollutants in mixed treatment are discussed. The electron transfer pathway revealed for the first time suggest that the mixed pollutants reduce the overall redox potentials and facilitate electron transfer during the plasma treatment, promoting synergistic degradation effects. The integrated frameworks including both direct and indirect mechanisms provide new insights into the high-efficiency mixed-contaminant treatment. The degradation products for mixed degradation are revealed based on the identification of intermediate species. The plasma-treated water is proven safe for living creatures in waterways and sustainable fishery applications, using in vivo zebrafish model bio-toxicity assay. Overall, these findings offer a feasible approach and new insights into the mechanisms for the development of highly-effective, energy-efficient technologies for wastewater treatment and reuse in agriculture, industry, and potentially in urban water networks.
Topics: Humans; Animals; Wastewater; Coloring Agents; Plasma Gases; Zebrafish; Water; Environmental Pollutants; Water Pollutants, Chemical
PubMed: 38199474
DOI: 10.1016/j.envres.2024.118125 -
Environmental Science and Pollution... Oct 2023Water pollution by synthetic anionic dyes is one of the most critical ecological concerns and challenges. Therefore, there is an urgent need to find an efficient...
Water pollution by synthetic anionic dyes is one of the most critical ecological concerns and challenges. Therefore, there is an urgent need to find an efficient adsorbent and photocatalyst for dye removal. In the present study, we aimed to fabricate a hybrid mesoporous composite of spongy sphere-like SnO and three-dimensional (3D) cubic-like MgO (SnO/MgO) as a promising adsorbent/photocatalyst to remove the anionic sunset yellow (SSY) dye from real wastewater at neutral pH conditions. The as-synthesized SnO and MgO composite was investigated using XRD, SEM, EDX, TEM, XPS, BET, and zeta potential. The experimental study of the SSY removal using SnO/MgO composite was performed at different conditions, such as pH, stirring time, dose, and temperature. More than 99% of 10 mg/L SSY was effectively adsorbed from aqueous solution using 40 mg of SnO/MgO composite at pH 7 and a stirring time of 60 min. The SSY adsorption behavior was well fitted by pseudo-second order and the Langmuir model, indicating that the SSY was chemisorbed to the composite-active sites as a monolayer. On the other hand, photocatalytic degradation process exhibited better results in terms of speed of removal and used quantity of photocatalyst, where 20 mg of SnO/MgO composite can be used to remove > 99% of SSY dye within 30 min. Mechanism of SSY adsorption and photocatalytic degradation was discussed. In addition, elution experiments demonstrated that the SnO/MgO composite as an SSY adsorbent could be reused for nine cycles without considerable reduction in the SSY adsorption efficiency. Therefore, this work exhibited that the mesoporous SnO/MgO composite can be considered an effective adsorbent/photocatalyst to remove SSY dye from real industrial effluent water at neutral pH conditions.
Topics: Coloring Agents; Magnesium Oxide; Adsorption; Water; Water Pollutants, Chemical; Kinetics; Hydrogen-Ion Concentration
PubMed: 37747604
DOI: 10.1007/s11356-023-29649-5 -
Analytical Chemistry Dec 2023Protein properties and interactions have been widely investigated by using external labels. However, the micromolar sensitivity of the current dyes limits their...
Protein properties and interactions have been widely investigated by using external labels. However, the micromolar sensitivity of the current dyes limits their applicability due to the high material consumption and assay cost. In response to this challenge, we synthesized a series of cyanine5 (Cy5) dye-based quencher molecules to develop an external dye technique to probe proteins at the nanomolar protein level in a high-throughput one-step assay format. Several families of Cy5 dye-based quenchers with ring and/or side-chain modifications were designed and synthesized by introducing organic small molecules or peptides. Our results showed that steric hindrance and electrostatic interactions are more important than hydrophobicity in the interaction between the luminescent negatively charged europium-chelate-labeled peptide (Eu-probe) and the quencher molecules. The presence of substituents on the quencher indolenine rings reduces their quenching property, whereas the increased positive charge on the indolenine side chain improved the interaction between the quenchers and the luminescent compound. The designed quencher structures entirely altered the dynamics of the Eu-probe (protein-probe) for studying protein stability and interactions, as we were able to reduce the quencher concentration 100-fold. Moreover, the new quencher molecules allowed us to conduct the experiments using neutral buffer conditions, known as the peptide-probe assay. These improvements enabled us to apply the method in a one-step format for nanomolar protein-ligand interaction and protein profiling studies instead of the previously developed two-step protocol. These improvements provide a faster and simpler method with lower material consumption.
Topics: Coloring Agents; Carbocyanines; Peptides; Luminescence; Fluorescent Dyes
PubMed: 38060502
DOI: 10.1021/acs.analchem.3c02844