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The Journal of Dermatological Treatment Dec 2023Onychopapilloma is a rare benign nail tumor affecting the distal matrix and the nail bed. Currently, the only available treatment is surgical resection, which has a...
Onychopapilloma is a rare benign nail tumor affecting the distal matrix and the nail bed. Currently, the only available treatment is surgical resection, which has a recurrence rate of 20% and may lead to various complications. Here we report a new method to treat onychopapilloma with pulsed dye laser (PDL). We retrospectively analyzed 13 cases and evaluated disease classification, dermoscopic examination, laser treatment parameters, photographs before and after treatment, and treatment outcome. The site distribution of onychopapilloma was consistent with previous reports. PDL treatment was performed with 595 nm laser, with 1.5 ms pulse duration, spot diameter 3-5 mm, and 11.5-13.5 J/cm fluence. Irradiation covered the telangiectatic area up to the edge of the nail folds, with the terminal response of purpura occurrence. The overall effective rate was 77%; the effective rates for erythronychia, leukonychia, and melanonychia were 88%, 67%, and 50%, respectively. PDL treatment for onychopapilloma provides an alternative to traditional surgery with comparable effectiveness but much less risk for complications.
Topics: Humans; Lasers, Dye; Retrospective Studies; Treatment Outcome; Purpura; Low-Level Light Therapy; Nail Diseases
PubMed: 37260138
DOI: 10.1080/09546634.2023.2213363 -
Scientific Reports Nov 2023The removal of toxic dye pigments from the environment is of utmost importance since even trace amounts of these pollutants can lead to harmful impacts on ecosystems....
The removal of toxic dye pigments from the environment is of utmost importance since even trace amounts of these pollutants can lead to harmful impacts on ecosystems. Heterogeneous photocatalysis is a potential technique for eliminating microbiological, inorganic, and organic pollutants from wastewater. Here, we report the band gap alteration of ZnO by making its composites with CuSe to enhance photocatalytic activity. The purpose is to develop metal oxide nanocomposites (ZnO/CuSe) as an effective and efficient material for the photodegradation of methyl blue. The photocatalysts, ZnO nanorods, CuSe, and ZnO/CuSe nanocomposites of different weight ratios were synthesized by the simple and cost-effective technique of precipitation. UV-Vis spectra verified that the ZnO/CuSe photocatalyst improved absorption in the visible region. The optical bandgap of ZnO/CuSe nanocomposites reduced from 3.37 to 2.68 eV when CuSe concentration increased from 10 to 50%. ZnO/CuSe composites demonstrated better photocatalytic activity than ZnO when exposed to UV-visible light. The pure ZnO nanorods could absorb UV light and the nanocomposites could absorb visible light only; this was attributed to the transfer of excited high-energy electrons from ZnO to CuSe.
PubMed: 37949952
DOI: 10.1038/s41598-023-46780-y -
ACS Applied Optical Materials Dec 2023The lasing characteristics of Thioflavin T (ThT) and Thioflavin X (ThX) dyes were investigated in solvents with increasing viscosity: water, ethanol, butanol, ethylene...
Cavity Lasing Characteristics of Thioflavin T and Thioflavin X in Different Solvents and Their Interaction with DNA for the Controlled Reduction of a Light Amplification Threshold in Solid-State Biofilms.
The lasing characteristics of Thioflavin T (ThT) and Thioflavin X (ThX) dyes were investigated in solvents with increasing viscosity: water, ethanol, butanol, ethylene glycol, and glycerol and three forms of DNA (double-helix natural, fragmented, and aggregated). The results identified that lasing thresholds and photostability depend on three critical factors: the solvation shell surrounding dye molecules, the organization of their dipole moments, which is driven by the DNA structure, and the molecules diffusion coefficient in the excitation focal spot. The research highlights that dye doped to DNA accumulated in binding sites fosters long-range dye orientation, facilitating a marked reduction of lasing thresholds in the liquid phase as well as amplified spontaneous emission (ASE) thresholds in the solid state. Leveraging insights from lasing characteristics obtained in liquid, ASE in the solid state was optimized in a controlled way by changing the parameters influencing the DNA structure, i.e., magnesium salt addition, heating, and sonication. The modifications led to a large decrease in the ASE thresholds in the dye-doped DNA films. It was shown that the examination of lasing in cavities can be useful for preparing optical materials with improved architectures and functionalities for solid-state lasers.
PubMed: 38149104
DOI: 10.1021/acsaom.3c00264 -
American Journal of Dentistry Feb 2024To investigate the differences between irrigant propagation and temperature changes using laser-activated irrigation (LAI) at different settings in an artificial root...
PURPOSE
To investigate the differences between irrigant propagation and temperature changes using laser-activated irrigation (LAI) at different settings in an artificial root canal model.
METHODS
Using an artificial resin root canal model, irrigant activation was achieved in 19 experimental groups with eight samples each. A 9,300 nm CO₂ laser, two diode lasers with different settings (wavelengths 455, 808, 970, and 980 nm) were compared to 2,940 nm Er:YAG laser and traditional needle irrigation. Er:YAG and CO₂ laser were activated in the pulpal chamber only, while diode lasers and needles were inserted into the main root canal. Lasers were activated for 5x 20 seconds resulting in 100 seconds of activation or rinsing for each sample. After each activation of 20 seconds, a photo was taken of the side canals and the propagation of the dye was measured with a digital measuring tool after calibration. Further, the temperature of the irrigant was reported after activation of 20 seconds and repeated 5 times. Data were checked for normality and statistically compared.
RESULTS
All lasers increased the irrigant propagation compared to conventional irrigation. Significant differences were found between groups regarding propagation and temperature (P< 0.0027). Er:YAG and CO₂ laser had similar effects on irrigant propagation in middle and apical located side-canals with specific power parameters and were superior to diode lasers and syringe irrigation. The irrigant's temperature increased significantly with the diode and CO₂ lasers.
CLINICAL SIGNIFICANCE
Diode lasers and CO₂ lasers have not been established for irrigant activation. 9,300 nm CO₂ lasers absorb well in water and were shown to introduce vapor bubble formation and streaming in water. Diode lasers are highly accepted in periodontics. The laser light is not absorbed in water but interacts with bacteria as well as soft tissues and contributes therefore to infection control. With a modified laser tip it was however possible to introduce cavitation and streaming in irrigants.
Topics: Carbon Dioxide; Lasers, Semiconductor; Proof of Concept Study; Root Canal Irrigants; Dental Pulp Cavity; Lasers, Solid-State; Water; Root Canal Preparation
PubMed: 38458982
DOI: No ID Found -
Facial Plastic Surgery & Aesthetic... 2024
Topics: Humans; Dermabrasion; Lasers, Dye; Nose
PubMed: 37205866
DOI: 10.1089/fpsam.2023.0054 -
Skin Research and Technology : Official... Sep 2023Viral warts, induced by infection by different HPV genotypes, are highly frequent in the general population, particularly during infancy and among adolescents. The...
BACKGROUND
Viral warts, induced by infection by different HPV genotypes, are highly frequent in the general population, particularly during infancy and among adolescents. The effectiveness of the 595 nm Pulse dye-laser in treating viral warts on the hands and feet in both adults and children was investigated in this study.
MATERIALS AND METHODS
A selection of 203 patients with multiple viral warts was made. All patients underwent 4-5 treatment sessions with a 595 nm pulse Dye Laser (PDL). Treatment outcome was assessed by a quartile scale: 1 indicates no or low results (0%-25% of the lesion area cleared), 2 indicates slight clearance (25%-50% of the lesion area cleared), 3 indicates moderate-good clearance (50%-75%), and 4 indicates excellent clearance (75%-100%). Patients were asked for a subjective evaluation of the perceived overall results by means of the following score: unsatisfied (1), not very satisfied (2), satisfied (3), and very satisfied (4). Possible side effects were monitored. Results obtained were judged with a photographic evaluation, immediately and at the control visit (6 months after the last laser session).
RESULTS
All patients observed global improvements. Most of the lesions were completely removed after laser therapy. A total of 95% of patients achieved excellent clearance and they were very satisfied following the laser treatment. Relevant side effects were absent in all patients.
CONCLUSION
PDL treatment with the study device using a wavelength of 595 nm has proven to be a tolerable and safe therapy for viral warts management.
Topics: Adolescent; Adult; Child; Humans; Lasers, Dye; Hand; Foot; Warts; Photography
PubMed: 37753676
DOI: 10.1111/srt.13460 -
Cytometry. Part a : the Journal of the... Mar 2024High dimensional flow cytometry relies on multiple laser sources to excite the wide variety of fluorochromes now available for immunophenotyping. Ultraviolet lasers...
High dimensional flow cytometry relies on multiple laser sources to excite the wide variety of fluorochromes now available for immunophenotyping. Ultraviolet lasers (usually solid state 355 nm) are a critical part of this as they excite the BD Horizon™ Brilliant Ultraviolet (BUV) series of polymer fluorochromes. The BUV dyes have increased the number of simultaneous fluorochromes available for practical high-dimensional analysis to greater than 40 for spectral cytometry. Immunologists are now seeking to increase this number, requiring both novel fluorochromes and additional laser wavelengths. A laser in the deep ultraviolet (DUV) range (from ca. 260 to 320 nm) has been proposed as an additional excitation source, driven by the on-going development of additional polymer dyes with DUV excitation. DUV lasers emitting at 280 and 320 nm have been previously validated for flow cytometry but have encountered practical difficulties both in probe excitation behavior and in availability. In this article, we validate an even shorter DUV 266 nm laser source for flow cytometry. This DUV laser provided minimal excitation of the BUV dyes (a desirable characteristic for high-dimensional analysis) while demonstrating excellent excitation of quantum nanoparticles (Qdots) serving as surrogate fluorochromes for as yet undeveloped DUV excited dyes. DUV 266 nm excitation may therefore be a viable candidate for expanding high-dimensional flow cytometry into the DUV range and providing an additional incidental excitation wavelength for spectral cytometry. Excitation in a spectral region with strong absorption by nucleic acids and proteins (260-280 nm) did result in strong autofluorescence requiring care in fluorochrome selection. DUV excitation of endogenous molecules may nevertheless have additional utility for label-free analysis applications.
Topics: Fluorescent Dyes; Flow Cytometry; Light; Lasers; Polymers
PubMed: 38116677
DOI: 10.1002/cyto.a.24813 -
Biomacromolecules Jul 2023A stimuli-responsive polymeric three-dimensional microstructured film (PTMF) is a 3D structure with an array of sealed chambers on its external surface. In this work, we...
A stimuli-responsive polymeric three-dimensional microstructured film (PTMF) is a 3D structure with an array of sealed chambers on its external surface. In this work, we demonstrate the use of PTMF as a laser-triggered stimulus-response system for local in vivo targeted blood vessels stimulation by vasoactive substances. The native vascular networks of the mouse mesentery were used as model tissues. Epinephrine and KCl were used as vasoactive agents that were sealed into individual chambers upon precipitation in the amount of pictograms. We demonstrated the method for non-damaged one-by-one chamber activation using a focused 532 nm laser light passed through biological tissues. To avoid laser-induced photothermal damage to biological tissues, the PTMF was functionalized with Nile Red dye, which effectively absorbs laser light. Chemically stimulated blood vessel fluctuations were analyzed using digital image processing methods. Hemodynamics changes were measured and visualized using the particle image velocimetry approach.
Topics: Mice; Animals; Lasers; Polymers; Infrared Rays
PubMed: 37289998
DOI: 10.1021/acs.biomac.3c00125 -
Physical Chemistry Chemical Physics :... Oct 2023Metal nanoparticles (NPs) can be employed to modify the emission level of a dye emitter by tailoring the spectral overlap of the optical gain and localized surface...
Metal nanoparticles (NPs) can be employed to modify the emission level of a dye emitter by tailoring the spectral overlap of the optical gain and localized surface plasmon resonance (LSPR). In the case of plasmonic random lasers, tuning the spectral overlap by manipulating metal NPs changes the scattering properties of the system, which is crucial in random lasers (RLs). In order to overcome this drawback, the emitter gain spectrum across the LSPR is tuned by appropriately choosing various dye emitters. A system with Au nanoislands (NIs) randomly distributed on the surface of vertically aligned ZnO nanorods on a glass substrate coated with three different dye emitters has been employed to study the metal-gain interaction as a function of spectral overlap. It is observed that the photoluminescence is quenched in the presence of Au NIs for all the three dye emitters; however, the degree of quenching is found to be directly proportional to the extent of spectral overlap of the LSPR and the fluorophore emission spectrum, with the resonantly coupled systems exhibiting higher random lasing thresholds. However, a dequenching of the emission is observed under spectrally off-resonant conditions, leading to a lower threshold RL. The effect of tailoring of the metal-gain interaction on the coherent and incoherent intensity components of RL emission is studied to elucidate the contrasting results of photoluminescence and RL emission. As the optical gain shifts away from the LSPR peak, the RL emission is dominated by the coherent intensity. The speckle-like field distributions of the RL modes couple to the plasmonic nanocavities along with a reduced absorption loss for the off-resonant case, leading to an enhanced stimulated emission. Hence, a synergy between random laser modes, plasmonic nanocavities and optimum spectral overlap has been utilized as a tool to dequench the plasmon quenched fluorophore emission.
PubMed: 37840472
DOI: 10.1039/d3cp04151d -
Indian Journal of Dental Research :... 2023Multiple accessory canals make furcation area of primary molars the most common port of entry of bacterial endotoxins to periradicular area. Reduction in permeability...
PURPOSE
Multiple accessory canals make furcation area of primary molars the most common port of entry of bacterial endotoxins to periradicular area. Reduction in permeability can improve prognosis of these teeth. Therefore, this study was designed to compare the effect of 940 nm diode laser and dentin bonding agent on the dye penetration of furcation area in primary molars.
MATERIAL AND METHODS
Thirty teeth were divided into three groups, that is, Group 1 (control group), Group 2 (dentin bonding agent applied over floor of pulp chamber), and Group 3 (diode laser irradiated over floor of pulp chamber). The samples were prepared and seven from each group were tested by dye penetration and three were sent for scanning electron microscopy. Rise in temperature in the perifurcal area was also recorded at the time of laser irradiation. Dunn's pair-wise comparison analysis was used for the analysis for the difference in dye penetration among the group.
RESULTS
: The maximum rise in temperature recorded postirradiation was 6.9C. Dye penetration was significantly reduced in Group 2 Dentin bonding group (DBG) as compared to control group (P = 0.0025). Reduction in permeability was observed in Group 3 Laser group (LG) but this was not statistically significant (P = 0.197). Scanning electron microscope revealed open dentinal tubules in Group 1. There were no open dentinal tubules in Group 2, while Group 3 had sealed dentinal tubules with glass-like surface over the region.
CONCLUSION
Dentin bonding agent led to substantial decrease in dye penetration of furcation area of primary molars. This method should be evaluated clinically to improve successful pulpectomy procedures of primary molars.
Topics: Lasers, Semiconductor; Permeability; Dental Pulp Cavity; Dentin-Bonding Agents; Molar
PubMed: 38197348
DOI: 10.4103/ijdr.ijdr_731_21