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Naunyn-Schmiedeberg's Archives of... Jun 2024In pharmaceutical industries, various chemical carriers are present which are used for drug delivery to the correct target sites. The most popular and upcoming drug... (Review)
Review
In pharmaceutical industries, various chemical carriers are present which are used for drug delivery to the correct target sites. The most popular and upcoming drug delivery carriers are mesoporous silica nanoparticles (MSN). The main reason for its popularity is its ability to be specific and optimize the drug delivery process in a controlled manner. Nowadays, MSNs are widely used to eradicate various microbial infections, especially the ones related to biofilms. Biofilms are sessile groups of cells that live by forming a consortium and exhibit antibacterial resistance (AMR). They exhibit AMR by extracellular polymeric substances (EPS) and various quorum sensing (QS) signaling molecules. Usually, bacterial and fungal cells are capable of forming biofilms. These biofilms are pathogenic. In the majority of the cases, biofilms cause nosocomial diseases. This review will focus on the antibiofilm activities of MSN, its mechanism of target-specific drug delivery, and its ability to disrupt the bacterial biofilms inhibiting the infection. The review will also discuss various mechanisms for the delivery of pharmaceutical molecules by the MSNs to inhibit the bacterial biofilms, and lastly, we will talk about the different types of MSNs and their antibiofilm activities.
Topics: Biofilms; Silicon Dioxide; Nanoparticles; Humans; Anti-Bacterial Agents; Animals; Porosity; Drug Carriers; Drug Delivery Systems; Bacterial Infections; Quorum Sensing; Bacteria
PubMed: 38051365
DOI: 10.1007/s00210-023-02872-0 -
Journal of Dairy Science Dec 2023Biofilms formed by pathogenic or spoilage microorganisms have become serious issues in the dairy industry, as this mode of life renders such microorganisms highly... (Review)
Review
Biofilms formed by pathogenic or spoilage microorganisms have become serious issues in the dairy industry, as this mode of life renders such microorganisms highly resistant to cleaning-in-place (CIP) procedures, disinfectants, desiccation, and other control strategies. The advent of omics techniques, especially the integration of different omics tools, has greatly improved our understanding of the features of microbial biofilms, and provided in-depth knowledge on developing effective methods that are directly against deleterious biofilms. This review provides novel insights into the single use of each omics tool and the application of multiomics tools to unravel the mechanisms of biofilm formation, specific molecular phenotypes exhibited by biofilms, and biofilm control strategies. Challenges and future perspective on the integration of omics tools for biofilm studies are also addressed.
Topics: Animals; Multiomics; Biofilms; Disinfectants; Dairying
PubMed: 37641326
DOI: 10.3168/jds.2023-23306 -
Journal of Materials Chemistry. B May 2024Currently, the rapid spread of multidrug-resistant bacteria derived from the indiscriminate use of traditional antibiotics poses a significant threat to public health... (Review)
Review
Currently, the rapid spread of multidrug-resistant bacteria derived from the indiscriminate use of traditional antibiotics poses a significant threat to public health worldwide. Moreover, established bacterial biofilms are extremely difficult to eradicate because of their high tolerance to traditional antimicrobial agents and extraordinary resistance to phagocytosis. Hence, it is of universal significance to develop novel robust and efficient antibacterial strategies to combat bacterial infections. Micro/nanomotors exhibit many intriguing properties, including enhanced mass transfer and micro-mixing resulting from their locomotion, intrinsic antimicrobial capabilities, active cargo delivery, and targeted treatment with precise micromanipulation, which facilitate the targeted delivery of antimicrobials to infected sites and their deep permeation into sites of bacterial biofilms for fast inactivation. Thus, the ideal antimicrobial activity of antibacterial micro/nanorobots makes them desirable alternatives to traditional antimicrobial treatments and has aroused extensive interest in recent years. In this review, recent advancements in antibacterial micro/nanomotors are briefly summarized, focusing on their synthetic methods, propulsion mechanism, and versatile antibacterial applications. Finally, some personal insights into the current challenges and possible future directions to translate proof-of-concept research to clinic application are proposed.
Topics: Humans; Anti-Bacterial Agents; Bacteria; Biofilms; Microbial Sensitivity Tests; Nanostructures; Bacterial Infections
PubMed: 38712692
DOI: 10.1039/d3tb02718j -
Journal of Environmental Management Oct 2023Algal-bacterial symbiotic system is a biological purification system that combines sewage treatment with resource utilization and has the dual effects of carbon...
Algal-bacterial symbiotic system is a biological purification system that combines sewage treatment with resource utilization and has the dual effects of carbon sequestration and pollution reduction. In this study, an immobilized algal-bacterial biofilm system was constructed for the treatment of natural sewage. Effects of exposure to microplastics (MPs) with different particle diameters (0.065 μm, 0.5 μm and 5 μm) were determined in terms of algal biomass recovery efficiency, the composition of extracellular polymeric substances (EPS) and morphologic characteristics. The impacts of MPs on the bacterial diversity and community structure of biofilms were also examined. The metagenomic analysis of key microorganisms and related metabolism pathways involved in system was further investigated. Results showed that following exposure to 5 μm MP, a maximum algal recovery efficiency of 80% was achieved, with a minimum PSII primary light energy conversion efficiency (F/F ratio) of 0.513. Furthermore, 5 μm MP caused the highest level of damage to the algal-bacterial biofilm, enhancing the secretion of protein-rich EPS. The biofilm morphology became rough and loose following exposure to 0.5 μm and 5 μm MP. Community diversity and richness were significantly high in biofilms exposed to 5 μm MP. Proteobacteria (15.3-24.1%), Firmicutes (5.0-7.8%) and Actinobacteria (4.2-4.9%) were dominant in all groups, with exposure to 5 μm MP resulting in the highest relative abundance for these species. The addition of MPs promoted the related metabolic functions while inhibited the degradation of harmful substances by algal-bacterial biofilms. The findings have environmental significance for the practical application of algal-bacterial biofilms for sewage treatment, providing novel insights into the potential effects of MPs on immobilized algal-bacterial biofilm systems.
Topics: Bacteria; Biofilms; Microbiota; Microplastics; Plastics; Sewage
PubMed: 37245312
DOI: 10.1016/j.jenvman.2023.118246 -
Trends in Microbiology Feb 2024Marine species, such as corals and kelp, are responding to climate change by altering their distributions. Microbial biofilms underpin key processes that affect the... (Review)
Review
Marine species, such as corals and kelp, are responding to climate change by altering their distributions. Microbial biofilms underpin key processes that affect the establishment, maintenance, and function of these dominant habitat-formers. Climate-mediated changes to microbial biofilms can therefore strongly influence species' range shifts. Here, we review emerging research on the interactions between benthic biofilms and habitat-formers and identify two key areas of interaction where climate change can impact this dynamic: (i) via direct effects on biofilm composition, and (ii) via impacts on the complex feedback loops which exist between the biofilm microbes and habitat-forming organisms. We propose that these key interactions will be fundamental in driving the speed and extent of tropicalisation of coastal ecosystems under climate change.
Topics: Animals; Ecosystem; Anthozoa; Climate Change; Aquatic Organisms; Biofilms; Coral Reefs
PubMed: 37633773
DOI: 10.1016/j.tim.2023.07.015 -
The Science of the Total Environment Jan 2024Microalgal-bacterial biofilms have been increasingly considered of great potential in wastewater treatment due to the advantages of microalgal-bacterial synergistic... (Review)
Review
Microalgal-bacterial biofilms have been increasingly considered of great potential in wastewater treatment due to the advantages of microalgal-bacterial synergistic pollutants removal/recovery, CO sequestration, and cost-effective biomass-water separation. However, such advantages may vary widely among different types of microalgal-bacterial biofilms, as the biofilms could be formed on different shapes and structures of attachment substratum, generating "false hope" for certain systems in large-scale wastewater treatment if the operating conditions and pollutants removal properties are evaluated based on the general term "microalgal-bacterial biofilm". This study, therefore, classified microalgal-bacterial biofilms into biofilms formed on 2D substratum, biofilms formed on 3D substratum, and biofilms formed without substratum (i.e. microalgal-bacterial granular sludge, MBGS). Biofilms formed on 2D substratum display higher microalgae fractions and nutrients removal efficiencies, while the adopted long hydraulic retention times were unacceptable for large-scale wastewater treatment. MBGS are featured with much lower microalgae fractions, most efficient pollutants removal, and acceptable retention times for realistic application, yet the feasibility of using natural sunlight should be further explored. 3D substratum systems display wide variations in operating conditions and pollutants removal properties because of diversified substratum shapes and structures. 2D and 3D substratum biofilms share more common in eukaryotic and prokaryotic microbial community structures, while MGBS biofilms are more enriched with microorganisms favoring EPS production, biofilm formation, and denitrification. The specific roles of stratified extracellular polymeric substances (EPS) in nutrients adsorption and condensation still require in-depth exploration. Nutrients removal uncertainties caused by microalgal-bacterial synergy decoupling under insufficient illumination, limited microbial community control, and possible greenhouse gas emission exacerbation arising from microalgal NO generation were also indicated. This review is helpful for revealing the true potential of applying various microalgal-bacterial biofilms in large-scale wastewater treatment, and will provoke some insights on the challenges to the ideal state of synergistic pollutants reclamation and carbon neutrality via microalgal-bacterial interactions.
Topics: Wastewater; Microalgae; Biofilms; Sewage; Bacteria; Biomass; Environmental Pollutants
PubMed: 37884155
DOI: 10.1016/j.scitotenv.2023.167974 -
Food Microbiology Sep 2024Biofilms formed by spoilage and pathogenic bacteria increase microbial persistence, causing an adverse influence on the quality of seafood. The mono-species biofilms are...
Biofilms formed by spoilage and pathogenic bacteria increase microbial persistence, causing an adverse influence on the quality of seafood. The mono-species biofilms are widely reported, however, the contamination of multi-species biofilms and their matrix in food environments are still not fully understood. Here, we assessed the contamination of multi-species biofilms in three seafood processing environments with different hygiene levels by detecting bacterial number and three biofilm matrix components (carbohydrates, extracellular DNA (eDNA), and proteins). Samples comprising seven food matrix surfaces and eight food processing equipment surfaces were collected from two seafood processing plants (XY and XC) and one seafood market (CC). The results showed that the bacterial counts ranged from 1.89 to 4.91 CFU/cm and 5.68 to 9.15 BCE/cm in these surfaces by cultivation and real-time PCR, respectively. Six biofilm hotspots were identified, including four in CC and two in XY. Among the three processing environments, the amplicon sequence variants (ASVs) of Proteobacteria, Bacteroidetes, and Actinobacteria decreased with improved processing hygiene, while Firmicutes showed a decrease in the four most abundant phyla. The most prevalent bacteria belonged to genera Psychrobacter, Acinetobacter, and Pseudomonas, demonstrating the significant differences and alteration in bacterial community composition during different environments. From the biofilm hotspots, 15 isolates with strong biofilm forming ability were identified, including 7 Pseudomonas, 7 Acinetobacter, and 1 Psychrobacter. The Pseudomonas isolates exhibited the highest production of EPS components and three strong motilities, whose characteristics were positively correlated. Thus, this study verified the presence of multi-species biofilms in seafood processing environments, offering preliminary insights into the diversity of microbial communities during processing. It highlights potential contamination sources and emphasizes the importance of understanding biofilms composition to control biofilms formation in seafood processing environments.
Topics: Biofilms; Seafood; Bacteria; Food Handling; Food Microbiology; Microbiota; Hygiene; Food Contamination
PubMed: 38839233
DOI: 10.1016/j.fm.2024.104553 -
Applied Biochemistry and Biotechnology Sep 2023Global water scarcity and water pollution necessitate wastewater reclamation for further use. As an alternative to conventional techniques, membrane technology is... (Review)
Review
Global water scarcity and water pollution necessitate wastewater reclamation for further use. As an alternative to conventional techniques, membrane technology is extensively used as an advanced method for water purification and wastewater treatment due to its selectivity, permeability, and efficient removal of pollutants. However, microbial biofouling is a major threat that deteriorates membrane performance and imparts operational challenges. It is a natural phenomenon caused by the undesirable colonization of microbes on membrane surfaces. The economic penalties associated with this menace are enormous. The traditional preventive measures are dominated by biocides, toxic chemicals, cleaners and antifouling surfaces, which are costly and often cause secondary pollution. Recent focus is thus being directed to promote inputs from nanotechnology to control and mitigate this major threat. Different anti-microbial nanomaterials can be effectively used to prevent the adhesion of microbes onto the membrane surfaces and eliminate microbial biofilms, to provide an economical and eco-friendly solution to biofouling. This review addresses the formation of microbial biofilms and biofouling in membrane operations. The potential of nanocomposite membranes in alleviating this problem and the challenges in commercialization are discussed. The antifouling mechanisms are also highlighted, which are not widely elucidated.
Topics: Biofouling; Wastewater; Water; Biofilms; Nanoparticles; Anti-Infective Agents; Membranes, Artificial; Water Purification
PubMed: 37093532
DOI: 10.1007/s12010-023-04497-8 -
International Journal of Molecular... Dec 2023Biofilms are composed of multicellular communities of microbial cells and their self-secreted extracellular polymeric substances (EPS). The viruses named bacteriophages...
Biofilms are composed of multicellular communities of microbial cells and their self-secreted extracellular polymeric substances (EPS). The viruses named bacteriophages can infect and lyze bacterial cells, leading to efficient biofilm eradication. The aim of this study was to analyze how bacteriophages disrupt the biofilm structure by killing bacterial cells and/or by damaging extracellular polysaccharides, proteins, and DNA. The use of colorimetric and spectrofluorimetric methods and confocal laser scanning microscopy (CLSM) enabled a comprehensive assessment of phage activity against biofilms. The impact of the phages vB_Efa29212_2e and vB_Efa29212_3e was investigated. They were applied separately or in combination on 1-day and 7-day-old biofilms. Phages 2e effectively inhibited the growth of planktonic cells with a limited effect on the biofilm. They did not notably affect extracellular polysaccharides and proteins; however, they increased DNA levels. Phages 3e demonstrated a potent and dispersing impact on biofilms, despite being slightly less effective than bacteriophages 2e against planktonic cells. Phages 3e reduced the amount of extracellular polysaccharides and increased eDNA levels in both 1-day-old and 7-day-old biofilm cultures. Phage cocktails had a strong antimicrobial effect on both planktonic and biofilm-associated bacteria. A significant reduction in the levels of polysaccharides, proteins, and eDNA in 1-day-old biofilm samples was noted, which confirms that phages interfere with the structure of biofilm by killing bacterial cells and affecting extracellular polymer levels.
Topics: Enterococcus faecalis; Bacteriophages; Biofilms; Polysaccharides; DNA
PubMed: 38139094
DOI: 10.3390/ijms242417260 -
Microbiological Research May 2024Pseudomonas aeruginosa is an opportunistic bacterial pathogen that causes life-threatening and persistent infections in immunocompromised patients. It is the culprit... (Review)
Review
Pseudomonas aeruginosa is an opportunistic bacterial pathogen that causes life-threatening and persistent infections in immunocompromised patients. It is the culprit behind a variety of hospital-acquired infections owing to its multiple tolerance mechanisms against antibiotics and disinfectants. Biofilms are sessile microbial aggregates that are formed as a result of the cooperation and competition between microbial cells encased in a self-produced matrix comprised of extracellular polymeric constituents that trigger surface adhesion and microbial aggregation. Bacteria in biofilms exhibit unique features that are quite different from planktonic bacteria, such as high resistance to antibacterial agents and host immunity. Biofilms of P. aeruginosa are difficult to eradicate due to intrinsic, acquired, and adaptive resistance mechanisms. Consequently, innovative approaches to combat biofilms are the focus of the current research. Nanocomposites, composed of two or more different types of nanoparticles, have diverse therapeutic applications owing to their unique physicochemical properties. They are emerging multifunctional nanoformulations that combine the desired features of the different elements to obtain the highest functionality. This review assesses the recent advances of nanocomposites, including metal-, metal oxide-, polymer-, carbon-, hydrogel/cryogel-, and metal organic framework-based nanocomposites for the eradication of P. aeruginosa biofilms. The characteristics and virulence mechanisms of P. aeruginosa biofilms, as well as their devastating impact and economic burden are discussed. Future research addressing the potential use of nanocomposites as innovative anti-biofilm agents is emphasized. Utilization of nanocomposites safely and effectively should be further strengthened to confirm the safety aspects of their application.
Topics: Humans; Pseudomonas aeruginosa; Biofilms; Anti-Bacterial Agents; Virulence; Pseudomonas Infections; Nanocomposites
PubMed: 38432017
DOI: 10.1016/j.micres.2024.127656