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Differentiation; Research in Biological... 2024Collagen is a highly abundant protein in the extracellular matrix of humans and mammals, and it plays a critical role in maintaining the body's structural integrity.... (Review)
Review
Collagen is a highly abundant protein in the extracellular matrix of humans and mammals, and it plays a critical role in maintaining the body's structural integrity. Type I collagen is the most prevalent collagen type and is essential for the structural integrity of various tissues. It is present in nearly all connective tissues and is the main constituent of the interstitial matrix. Mutations that affect collagen fiber formation, structure, and function can result in various bone pathologies, underscoring the significance of collagen in sustaining healthy bone tissue. Studies on type 1 collagen have revealed that mutations in its encoding gene can lead to diverse bone diseases, such as osteogenesis imperfecta, a disorder characterized by fragile bones that are susceptible to fractures. Knowledge of collagen's molecular structure, synthesis, assembly, and breakdown is vital for comprehending embryonic and foetal development and several aspects of human physiology. In this review, we summarize the structure, molecular biology of type 1 collagen, its biomineralization and pathologies affecting bone.
Topics: Animals; Humans; Collagen Type I; Calcification, Physiologic; Collagen; Osteogenesis Imperfecta; Bone and Bones; Mutation; Mammals
PubMed: 38437764
DOI: 10.1016/j.diff.2024.100757 -
Nature Biomedical Engineering Nov 2023In patients with breast cancer, lower bone mineral density increases the risk of bone metastasis. Although the relationship between bone-matrix mineralization and...
In patients with breast cancer, lower bone mineral density increases the risk of bone metastasis. Although the relationship between bone-matrix mineralization and tumour-cell phenotype in breast cancer is not well understood, mineralization-induced rigidity is thought to drive metastatic progression via increased cell-adhesion forces. Here, by using collagen-based matrices with adjustable intrafibrillar mineralization, we show that, unexpectedly, matrix mineralization dampens integrin-mediated mechanosignalling and induces a less proliferative stem-cell-like phenotype in breast cancer cells. In mice with xenografted decellularized physiological bone matrices seeded with human breast tumour cells, the presence of bone mineral reduced tumour growth and upregulated a gene-expression signature that is associated with longer metastasis-free survival in patients with breast cancer. Our findings suggest that bone-matrix changes in osteogenic niches regulate metastatic progression in breast cancer and that in vitro models of bone metastasis should integrate organic and inorganic matrix components to mimic physiological and pathologic mineralization.
Topics: Humans; Mice; Animals; Female; Bone Matrix; Integrins; Breast Neoplasms; Calcification, Physiologic; Collagen; Mammary Neoplasms, Animal; Calcinosis
PubMed: 37550422
DOI: 10.1038/s41551-023-01077-3 -
Environmental Research Aug 2023Amphibole minerals are found throughout nature and could pose a respiratory hazard if these exist in the asbestiform growth habit. Though amphibole asbestos has not been...
Amphibole minerals are found throughout nature and could pose a respiratory hazard if these exist in the asbestiform growth habit. Though amphibole asbestos has not been used in commercial products as an added material for more than 30 years, these minerals could exist in other materials as trace contaminants as well as occurring in mines and earth-moving environments. It is necessary, then, that the asbestiform amphibole minerals be properly identified in order to appropriately use health-protective measures. Recent analyses of various amphibole data sets have been used to derive a discriminant function that can be used to differentiate asbestiform amphibole from non-asbestiform amphibole minerals. This paper expands on this function and examines the validity of the procedure for different size fractions of minerals. This analysis suggests that the derived function is appropriate for fibers 10 μm and longer. For fibers shorter than 10 μm, the data suggest that a broader acceptance limit may be needed. The data also suggest that current analytical procedures may require some adjustment to provide more accurate details on the widths of fibers. With additional samples, the accuracy of the discriminate function can be improved by calculating functions for each mineral.
Topics: Asbestos, Amphibole; Discriminant Analysis; Minerals; Mining
PubMed: 36965796
DOI: 10.1016/j.envres.2022.114579 -
Journal of Cellular Physiology Sep 2023Repair of orthodontic external root resorption and periodontal tissue dysfunction induced by mechanical force remains a clinical challenge. Cementoblasts are vital in...
Repair of orthodontic external root resorption and periodontal tissue dysfunction induced by mechanical force remains a clinical challenge. Cementoblasts are vital in cementum mineralization, a process important for restoring damaged cementum. Despite autophagy plays a role in mineralization under various environmental stimuli, the underlying mechanism of autophagy in mediating cementoblast mineralization remains unclear. Here we verified that murine cementoblasts exhibit compromised mineralization under compressive force. Autophagy was indispensable for cementoblast mineralization, and autophagic activation markedly reversed cementoblast mineralization and prevented cementum damage in mice during tooth movement. Subsequently, messenger RNA sequencing analyses identified periostin (Postn) as a mediator of autophagy and mineralization in cementoblasts. Cementoblast mineralization was significantly inhibited following the knockdown of Postn. Furthermore, Postn silencing suppressed Wnt signaling by modulating the stability of β-catenin. Together our results highlight the role of autophagy in cementoblast mineralization via Postn/β-catenin signaling under compressive force and may provide a new strategy for the remineralization of cementum and regeneration of periodontal tissue.
Topics: Animals; Mice; beta Catenin; Cell Differentiation; Cell Line; Dental Cementum; Wnt Signaling Pathway; Calcification, Physiologic; Cell Adhesion Molecules; Autophagy; Compressive Strength
PubMed: 37475648
DOI: 10.1002/jcp.31075 -
Microbial Biotechnology Sep 2023Biomineralization, the capacity to form minerals, has evolved in a great diversity of bacterial lineages as an adaptation to different environmental conditions and...
Biomineralization, the capacity to form minerals, has evolved in a great diversity of bacterial lineages as an adaptation to different environmental conditions and biological functions. Microbial biominerals often display original properties (morphology, composition, structure, association with organics) that significantly differ from those of abiotically formed counterparts, altogether defining the 'mineral phenotype'. In principle, it should be possible to take advantage of microbial biomineralization processes to design and biomanufacture advanced mineral materials for a range of technological applications. In practice, this has rarely been done so far and only for a very limited number of biomineral types. This is mainly due to our poor understanding of the underlying molecular mechanisms controlling microbial biomineralization pathways, preventing us from developing bioengineering strategies aiming at improving biomineral properties for different applications. Another important challenge is the difficulty to upscale microbial biomineralization from the lab to industrial production. Addressing these challenges will require combining expertise from environmental microbiologists and geomicrobiologists, who have historically been working at the forefront of research on microbe-mineral interactions, alongside bioengineers and material scientists. Such interdisciplinary efforts may in the future allow the emergence of a mineral biomanufacturing industry, a critical tool towards the development more sustainable and circular bioeconomies.
Topics: Minerals; Bacteria
PubMed: 37522764
DOI: 10.1111/1751-7915.14298 -
Bone Mar 2024Bone Mineral Density (BMD) is an important parameter in the development of orthopedic fracture-healing methods. A recent article (Inoue, S., et al. Bone. 2023, 177,...
Bone Mineral Density (BMD) is an important parameter in the development of orthopedic fracture-healing methods. A recent article (Inoue, S., et al. Bone. 2023, 177, 116916) investigated the use of higher intensity ultrasound to promote murine bone formation by measuring BMD levels. In this work, we present the numerical values of BMD, which show sigmoid kinetics and hyperbolic asymptotic increase with the application of higher intensity ultrasound. Our analysis may provide a foundation for the understanding and application of ultrasound to the human body.
Topics: Humans; Mice; Animals; Calcification, Physiologic; Bone Density; Ultrasonography; Bone and Bones; Osteogenesis
PubMed: 38158169
DOI: 10.1016/j.bone.2023.116999 -
Frontiers in Endocrinology 2024
Topics: Humans; Minerals; Ions; Metabolic Diseases; Endocrine System
PubMed: 38633752
DOI: 10.3389/fendo.2024.1391096 -
Acta Biomaterialia Jul 2023The hierarchical design of bio-based nanostructured materials such as bone enables them to combine unique structure-mechanical properties. As one of its main components,...
The hierarchical design of bio-based nanostructured materials such as bone enables them to combine unique structure-mechanical properties. As one of its main components, water plays an important role in bone's material multiscale mechanical interplay. However, its influence has not been quantified at the length-scale of a mineralised collagen fibre. Here, we couple in situ micropillar compression, and simultaneous synchrotron small angle X-ray scattering (SAXS) and X-ray diffraction (XRD) with a statistical constitutive model. Since the synchrotron data contain statistical information on the nanostructure, we establish a direct connection between experiment and model to identify the rehydrated elasto-plastic micro- and nanomechanical fibre behaviour. Rehydration led to a decrease of 65%-75% in fibre yield stress and compressive strength, and 70% in stiffness with a 3x higher effect on stresses than strains. While in agreement with bone extracellular matrix, the decrease is 1.5-3x higher compared to micro-indentation and macro-compression. Hydration influences mineral more than fibril strain with the highest difference to the macroscale when comparing mineral and tissue levels. The effect of hydration seems to be strongly mediated by ultrastructural interfaces while results provide insights towards mechanical consequences of reported water-mediated structuring of bone apatite. The missing reinforcing capacity of surrounding tissue for an excised fibril array is more pronounced in wet than dry conditions, mainly related to fibril swelling. Differences leading to higher compressive strength between mineralised tissues seem not to depend on rehydration while the lack of kink bands supports the role of water as an elastic embedding influencing energy-absorption mechanisms. STATEMENT OF SIGNIFICANCE: Characterising structure-property-function relationships in hierarchical biological materials helps us to elucidate mechanisms that enable their unique properties. Experimental and computational methods can advance our understanding of their complex behaviour with the potential to inform bio-inspired material development. In this study, we close a gap for bone's fundamental mechanical building block at micro- and nanometre length scales. We establish a direct connection between experiments and simulations by coupling in situ synchrotron tests with a statistical model and quantify the behaviour of rehydrated single mineralised collagen fibres. Results suggest a high influence of hydration on structural interfaces, and the role of water as an elastic embedding by outlining important differences between wet and dry elasto-plastic properties of mineral nanocrystals, fibrils and fibres.
Topics: Scattering, Small Angle; Stress, Mechanical; X-Ray Diffraction; Minerals; Collagen
PubMed: 37059408
DOI: 10.1016/j.actbio.2023.03.041 -
Journal of Bone and Mineral Metabolism Nov 2023
Topics: Bone and Bones; Awards and Prizes; Minerals
PubMed: 38008802
DOI: 10.1007/s00774-023-01483-2 -
Journal of Trace Elements in Medicine... Sep 2023Biomedical application is based on the use of LIBS-derived data on chemical contents of tissues in diagnosis of diseases, forensic investigation, as well as a mechanism... (Review)
Review
Medical application of laser-induced breakdown spectroscopy (LIBS) for assessment of trace element and mineral in biosamples: Laboratory and clinical validity of the method.
BACKGROUND
Biomedical application is based on the use of LIBS-derived data on chemical contents of tissues in diagnosis of diseases, forensic investigation, as well as a mechanism for providing online feedback for laser surgery. Although LIBS has certain advantages, the issue of correlation of LIBS-derived data on chemical element content in different human and animal tissues with other methods, and especially ICP-MS, remains pertinent. The objective of the present review was to discuss the application of laser-induced breakdown spectroscopy (LIBS) for elemental analysis of human biosamples or tissues from experimental models of human diseases.
METHODS
A systematic search in the PubMed-Medline, Scopus, and Google Scholar databases using the terms laser-induced breakdown spectroscopy, LIBS, metals, trace elements, minerals, and names of particular chemical elements was performed up through 25 February, 2023. Of all extracted studies only those dealing with human subjects, human tissues, in vivo animal and in vitro cell line models of human diseases were reviewed in detail.
RESULTS
The majority of studies revealed a wide number of metals and metalloids in solid tissues including teeth (As, Ag, Ca, Cd, Cr, Cu, Fe, Hg, Mg, Ni, P, Pb, Sn, Sr, Ti, and Zn), bones (Al, Ba, Ca, Cd, Cr, K, Mg, Na, Pb, Sr), and nails (Al, As, Ca, Fe, K, Mg, Na, P, Pb, Si, Sr, Ti, Zn). At the same time, LIBS was also used for estimation of trace element and mineral content in hair (Ca, Cu, Fe, K, Mg, Na, Zn), blood (Al, Ca, Co, Cd, Cu, Fe, Mg, Mn, Ni, Pb, Si, Sn, Zn), cancer tissues (Ca, Cu, Fe, Mg, K, Na, Zn) and other tissues. Single studies revealed satisfactory correspondence between quantitative LIBS and ICP-OES/MS data on the level of As (81-93 %), Pb (94-98 %), Cd (50-94 %) in teeth, Cu (97-105 %), Fe (117 %), Zn (88-117 %) in hair, Ca (97-99 %), Zn (90-95 %), and Pb (61-82 %) in kidney stones. LIBS also estimated specific patterns of trace element and mineral content associated with multiple pathologies, including caries, cancer, skin disorders, and other systemic diseases including diabetes mellitus type 2, osteoporosis, hypothyroidism, etc. Data obtained from in situ tissue LIBS analysis were profitably used for discrimination between tissue types.
CONCLUSIONS
Taken together, the existing data demonstrate the applicability of LIBS for medical studies, although further increase in its sensitivity, calibration range, cross-validation, and quality control is required.
Topics: Animals; Humans; Trace Elements; Cadmium; Lead; Minerals; Spectrum Analysis
PubMed: 37393771
DOI: 10.1016/j.jtemb.2023.127241