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Ultrastructural Pathology Mar 2024Radiation exposure is a major health concern due to bone involvement including mandible, causing deleterious effects on bone metabolism, and healing with an increasing...
Radiation exposure is a major health concern due to bone involvement including mandible, causing deleterious effects on bone metabolism, and healing with an increasing risk of infection and osteoradionecrosis. This study aims to investigate the radiotherapy-induced microstructural changes in the human mandible by scanning electron microscopy (SEM). Mandibular cortical bone biopsies were obtained from control, irradiated, and patients with osteoradionecrosis (ORN). Bone samples were prepared for light microscopy and SEM. The SEM images were analyzed for the number of osteons, number of Haversian canal (HC), diameter of osteon (D.O), the diameter of HC (D.HC), osteonal wall thickness (O.W.Th), number of osteocytes, and number of osteocytic dendrites. The number of osteons, D.O, D.HC, O.W.Th, the number of osteocytes, and osteocytic dendrites were significantly decreased in both irradiated and ORN compared to controls ( < .05). The number of HCs decreased in irradiated and ORN bone compared to the control group. However, this was statistically not significant. The deleterious effect of radiation continues gradually altering the bone quality, structure, cellularity, and vascularity in the long term (>5 years mean radiation biopsy interval). The underlying microscopic damage in bone increases its susceptibility and contributes further to radiation-induced bone changes or even ORN.
Topics: Humans; Microscopy, Electron, Scanning; Osteoradionecrosis; Osteocytes; Haversian System; Mandible
PubMed: 38115187
DOI: 10.1080/01913123.2023.2295458 -
Acta Biomaterialia Sep 2023The development of treatment strategies for skeletal diseases relies on the understanding of bone mechanical properties in relation to its structure at different length...
The development of treatment strategies for skeletal diseases relies on the understanding of bone mechanical properties in relation to its structure at different length scales. At the microscale, indention techniques can be used to evaluate the elastic, plastic, and fracture behaviour of bone tissue. Here, we combined in situ high-resolution SRµCT indentation testing and digital volume correlation to elucidate the anisotropic crack propagation, deformation, and fracture of ovine cortical bone under Berkovich and spherical tips. Independently of the indenter type we observed significant dependence of the crack development due to the anisotropy ahead of the tip, with lower strains and smaller crack systems developing in samples indented in the transverse material direction, where the fibrillar bone ultrastructure is largely aligned perpendicular to the indentation direction. Such alignment allows to accommodate the strain energy, inhibiting crack propagation. Higher tensile hoop strains generally correlated with regions that display significant cracking radial to the indenter, indicating a predominant Mode I fracture. This was confirmed by the three-dimensional analysis of crack opening displacements and stress intensity factors along the crack front obtained for the first time from full displacement fields in bone tissue. The X-ray beam significantly influenced the relaxation behaviour independent of the tip. Raman analyses did not show significant changes in specimen composition after irradiation compared to non-irradiated tissue, suggesting an embrittlement process that may be linked to damage of the non-fibrillar organic matrix. This study highlights the importance of three-dimensional investigation of bone deformation and fracture behaviour to explore the mechanisms of bone failure in relation to structural changes due to ageing or disease. STATEMENT OF SIGNIFICANCE: Characterising the three-dimensional deformation and fracture behaviour of bone remains essential to decipher the interplay between structure, function, and composition with the aim to improve fracture prevention strategies. The experimental methodology presented here, combining high-resolution imaging, indentation testing and digital volume correlation, allows us to quantify the local deformation, crack propagation, and fracture modes of cortical bone tissue. Our results highlight the anisotropic behaviour of osteonal bone and the complex crack propagation patterns and fracture modes initiating by the intricate stress states beneath the indenter tip. This is of wide interest not only for the understanding of bone fracture but also to understand other architectured (bio)structures providing an effective way to quantify their toughening mechanisms in relation to their main mechanical function.
Topics: Sheep; Animals; Synchrotrons; Anisotropy; Bone and Bones; Cortical Bone; Fractures, Bone; Stress, Mechanical
PubMed: 37127075
DOI: 10.1016/j.actbio.2023.04.038 -
Heliyon Sep 2023In orthodontic procedures, mini-implants are routinely used as temporary anchorage devices. Early failure is primarily attributed to a variety of issues, which are...
BACKGROUND
In orthodontic procedures, mini-implants are routinely used as temporary anchorage devices. Early failure is primarily attributed to a variety of issues, which are mostly connected to the quality and geometry of the screw that lead to insufficient primary stability.
OBJECTIVES
To evaluate the primary stability of different sizes and brands of orthodontic mini-implants by optimizing the insertion torque value (ITV) and to clear out which one has the greatest primary stability among the most widely used mini-implants by orthodontists.
METHODS
Eighty-two self-drilling mini-implants from three different brands with different sizes were used (Optimus Ortho System (Osteonic made in Korea), Smart anchor (GNI made in Korea) (1.4 × 6, 1.6 × 8 and 1.8 × 10mm) and Morelli (made in Brazil) (1.5 × 6, 1.5 × 8 and 1.5 × 10mm), made from (Ti 6Al 4V). All were drilled at a 60° angle on Sixty artificial bone blocks made from polyurethane foam with a digital torque meter device (Orthonia, Jeil made in Korea), pullout strength (tensile force) was measured with a universal testing machine to find out the best brand and size in the mean of primary stability. Data were analyzed using SPSS Version 25 and JMP Pro Version 16 software using the One-way ANOVA test, the Post hoc and Tukey HCD tests.
RESULTS
There were significant differences between the pullout strength of different sizes for the GNI and OSTEONIC brands, while for the MORELLI brand there were no significant differences between the three different sizes considering ITV (10Ncm) whereas for ITV (20Ncm) there was a significant difference between the different sizes for the pullout of all three brands. GNI was the best brand for all the selected sizes with ITV (10Ncm) and size 1.4 × 6 for ITV (20Ncm), whereas OSTEONIC sizes 1.6 × 8 and 1.8 × 10 were the best for ITV (20Ncm) in term of primary stability.
CONCLUSION
GNI screws were demonstrated higher primary among the three widely used brands followed by OSTEONIC for size 1.6 × 8 and 1.8 × 10 while MORELLI was the least resistant to dislodgement for the two torque insertion values 10 N/cm and 20 N/cm.
PubMed: 37809894
DOI: 10.1016/j.heliyon.2023.e19858 -
Journal of Advanced Research Dec 2023The bone ingrowth depth in the porous scaffolds is greatly affected by the structural design, notably the pore size, pore geometry, and the pore distribution. To enhance...
INTRODUCTION
The bone ingrowth depth in the porous scaffolds is greatly affected by the structural design, notably the pore size, pore geometry, and the pore distribution. To enhance the bone regeneration capability of scaffolds, the bionic design can be regarded as a potential solution.
OBJECTIVES
We proposed a Haversian system-like gradient structure based on the triply periodic minimal surface architectures with pore size varying from the edge to the center. And its effects in promoting bone regeneration were evaluated in the study.
METHODS
The gradient scaffold was designed using the triply periodic minimal surface architectures. The mechanical properties were analyzed by the finite element simulation and confirmed using the universal machine. The fluid characteristics were calculated by the computational fluid dynamics analysis. The bone regeneration process was simulated using a in silico computational model containing the main biological, physical, and chemical variation during the bone growth process. Finally, the in vitro and in vivo studies were carried out to verify the actual osteogenic effect.
RESULTS
Compared to the uniform scaffold, the biomimetic gradient scaffold demonstrated better performance in stress conduction and reduced stress shielding effects. The fluid features were appropriate for cell migration and flow diffusion, and the permeability was in the same order of magnitude with the natural bone. The bone ingrowth simulation exhibited improved angiogenesis and bone regeneration. Higher expression of the osteogenesis-related genes, higher alkaline phosphatase activity, and increased mineralization could be observed on the gradient scaffold in the in vitro study. The 12-week in vivo study proved that the gradient scaffold had deeper bone inserting depth and a more stable bone-scaffold interface.
CONCLUSION
The Haversian system-like gradient structure can effectively promote the bone regeneration. This structural design can be used as a new solution for the clinical application of prosthesis design.
Topics: Tissue Scaffolds; Porosity; Haversian System; Osteogenesis; Bone Regeneration
PubMed: 36632888
DOI: 10.1016/j.jare.2023.01.004 -
Journal of the Mechanical Behavior of... Aug 2023The research focuses on the evaluation of the mechanical properties of osteonal cortical bone at the lamellar level. Elastic properties of the mid-diaphysis region of...
The research focuses on the evaluation of the mechanical properties of osteonal cortical bone at the lamellar level. Elastic properties of the mid-diaphysis region of the bovine tibia are investigated via cantilever-based nanoindentation at the submicron length scale utilizing Atomic Force Microscopy, where the force-displacement curves are used for the elastic assessment using the Derjaguin-Muller-Toropov model to calculate indentation modulus. Variations of the modulus and the directional mechanical response of the osteonal bone at different distances from the Haversian canal are investigated. Additionally, the effects of demineralization on the indentation modulus are discussed. It was found that in the axial direction, the first and last untreated thick lamella layers show a significant indentation modulus difference compared to all other layers (4.26 ± 0.4 and 4.6 ± 0.3 GPa vs ∼3.5 GPa). On the other hand, the indentation modulus of transverse thick lamella layers shows a periodic variation between ∼3 ± 0.7 GPa and ∼4 ± 0.3 GPa from near the Haversian canal to near the interstitial bone. A periodic variation in the anisotropy ratio was found. Mineral content was quantified via energy-dispersive X-ray microanalysis at different levels of mineralization and shows a positive correlation with the indentation modulus.
Topics: Animals; Cattle; Tibia; Anisotropy; Bone and Bones; Cortical Bone; Elasticity
PubMed: 37393887
DOI: 10.1016/j.jmbbm.2023.105992 -
ACS Nano Dec 2023Mineralized collagen fibrils are the building block units of bone at the nanoscale. While it is known that collagen fibrils are mineralized both inside their gap zones...
Mineralized collagen fibrils are the building block units of bone at the nanoscale. While it is known that collagen fibrils are mineralized both inside their gap zones (intra-fibrillar mineralization) and on their outer surfaces (extra-fibrillar mineralization), a clear visualization of this architecture in three dimensions (3D), combining structural and compositional information over large volumes, but without compromising the resolution, remains challenging. In this study, we demonstrate the use of on-axis -contrast electron tomography (ET) with correlative energy-dispersive X-ray spectroscopy (EDX) tomography to examine rod-shaped samples with diameters up to 700 nm prepared from individual osteonal lamellae in the human femur. Our work mainly focuses on two aspects: (i) low-contrast nanosized circular spaces ("holes") observed in sections of bone oriented perpendicular to the long axis of a long bone, and (ii) extra-fibrillar mineral, especially in terms of morphology and spatial relationship with respect to intra-fibrillar mineral and collagen fibrils. From our analyses, it emerges quite clearly that most "holes" are cross-sectional views of collagen fibrils. While this had been postulated before, our 3D reconstructions and reslicing along meaningful two-dimensional (2D) cross-sections provide a direct visual confirmation. Extra-fibrillar mineral appears to be composed of thin plates that are interconnected and span over several collagen fibrils, confirming that mineralization is cross-fibrillar, at least for the extra-fibrillar phase. EDX tomography shows mineral signatures (Ca and P) within the gap zones, but the signal appears weaker than that associated with the extra-fibrillar mineral, pointing toward the existence of dissimilarities between the two types of mineralization.
Topics: Humans; Cross-Sectional Studies; Electrons; Spectrum Analysis; Electron Microscope Tomography; Collagen; Minerals
PubMed: 37846873
DOI: 10.1021/acsnano.3c04633 -
Computer Methods in Biomechanics and... 2024The bone lacunar-canalicular system (LCS) is a unique complex 3D microscopic tubular network structure within the osteon that contains interstitial fluid flow to ensure...
The bone lacunar-canalicular system (LCS) is a unique complex 3D microscopic tubular network structure within the osteon that contains interstitial fluid flow to ensure the efficient transport of signaling molecules, nutrients, and wastes to guarantee the normal physiological activities of bone tissue. The mass transfer laws in the LCS under microgravity and hypergravity are still unclear. In this paper, a multi-scale 3D osteon model was established to mimic the cortical osteon, and a finite element method was used to numerically analyze the mass transfer in the LCS under hypergravity, normal gravity and microgravity and combined with high-intensity exercise conditions. It was shown that hypergravity promoted mass transfer in the LCS to the deep lacunae, and the number of particles in lacunae increased more significantly from normal gravity to hypergravity the further away from the Haversian canal. The microgravity environment inhibited particles transport in the LCS to deep lacunae. Under normal gravity and microgravity, the number of particles in lacunae increased greatly when doing high-intensity exercise compared to stationary standing. This paper presents the first simulation of mass transfer within the LCS with different gravity fields combined with high-intensity exercise using the finite element method. The research suggested that hypergravity can greatly promote mass transfer in the LCS to deep lacunae, and microgravity strongly inhibited this mass transfer; high-intensity exercise increased the mass transfer rate in the LCS. This study provided a new strategy to combat and treat microgravity-induced osteoporosis.
Topics: Hypergravity; Weightlessness; Bone and Bones; Computer Simulation
PubMed: 36912751
DOI: 10.1080/10255842.2023.2187738 -
Dental Research Journal 2023This study compared the effect of various grafting materials on the area and volume of minerals attached to dental implants.
BACKGROUND
This study compared the effect of various grafting materials on the area and volume of minerals attached to dental implants.
MATERIALS AND METHODS
In this animal study, 13 dogs were divided into three groups according to the time of sacrificing (2 months, 4 months, or 6 months). The implants were placed in oversized osteotomies, and the residual defects were filled with autograft, bovine bone graft (Cerabone), or a synthetic substitute (Osteon II). At the designated intervals, the dogs were sacrificed and the segmented implants underwent micro-computed tomography analysis. The bone-implant area (BIA) and bone-implant volume (BIV) of bone and graft material were calculated in the region of interest around the implant. The data were analyzed by two-way analysis of variance (ANOVA) at < 0.05.
RESULTS
There was no significant difference in BIA and BIV between the healing intervals for any of the grafting materials ( > 0.05). ANOVA exhibited comparable BIA and BIV between the grafting materials at 2 and 4 months after surgery ( > 0.05), although a significant difference was observed after 6 months ( < 0.05). Pairwise comparisons revealed that BIA was significantly greater in the autograft-stabilized than the synthetic-grafted sites ( = 0.035). The samples augmented with autograft also showed significantly higher BIV than those treated by the xenogenic ( = 0.017) or synthetic ( = 0.002) particles.
CONCLUSION
All graft materials showed comparable performance in providing mineral support for implants up to 4 months after surgery. At the long-term (6-month) interval, autogenous bone demonstrated significant superiority over xenogenic and synthetic substitutes concerning the bone area and volume around the implant.
PubMed: 38020252
DOI: No ID Found -
The International Journal of Oral &... Jun 2024To answer the following focus question: In preclinical in vivo experimental models, do oral implants placed in overdimensioned (OD) sites present greater biomechanical... (Meta-Analysis)
Meta-Analysis
PURPOSE
To answer the following focus question: In preclinical in vivo experimental models, do oral implants placed in overdimensioned (OD) sites present greater biomechanical properties and histomorphometric parameters of osseointegration compared to implants placed in standard or undersized implant sockets?
MATERIALS AND METHODS
Online databases were searched for controlled animal studies reporting on OD sites up to February 2023. The relative implant- final drill discrepancy (IDD) was used to categorize the control and test groups according to surgical drilling protocol: (1) control: undersized (IDD > 0.5 mm) or standard (IDD = 0.2 to 0.5 mm); and (2) test OD: stress-free oversized (IDD = 0.0 to -0.1 mm); test GAP: friction-free oversized (IDD ≤ -0.1 mm). Random-effects meta-analyses were performed for the outcomes of insertion and removal torque values (ITV and RTV, respectively), bone-to-implant contact (%BIC), and bone density (%BD) for short- (0 to 2 weeks), intermediate- (3 to 4 weeks), and long-term (≥ 5 weeks) healing periods.
RESULTS
Of the 527 records identified, 13 studies met the eligibility criteria. Histologically, the OD and GAP groups prevented ischemic necrosis and extensive bone resorption at the bone-implant interface in both the marginal cortical layer and the trabeculae. Faster and increased rates of bone formation, characterized by primary osteons and highly vascularized tissue, took place in OD sites between 1 and 5 weeks of healing. Meta-analyses indicated statistically significant benefits in favor of (1) control vs OD for short-term healing in extraoral sites, with pooled estimates (weighted mean difference) of ITV = 25.35 Ncm, %BIC = 2.10%, and %BD = 26.19%; (2) control vs OD for long-term healing in intraoral sites, with %BD = 11.69%; (3) control vs GAP for intermediate-term healing in extraoral sites, with %BD = 3.03%; and (4) control vs GAP for long-term healing in extraoral sites, with RTV = 5.57 Ncm.
CONCLUSIONS
Oversized surgical preparation of the implant site does not seem to provide any additional benefit compared to standard or undersized sites regarding quantitative parameters of osseointegration. However, it does minimize marginal bone resorption and yields better-quality bone healing, despite the comparable results among different experimental animal models in the late postoperative period.
Topics: Dental Implantation, Endosseous; Osseointegration; Animals; Dental Implants; Biomechanical Phenomena; Torque
PubMed: 38905117
DOI: 10.11607/jomi.10059 -
Bulletin of Experimental Biology and... Dec 2023Stimulation of neoosteogenesis is the main mechanism of osseointegration during installation of dental implants, bone tissue recession, and alveolar process augmentation...
Stimulation of neoosteogenesis is the main mechanism of osseointegration during installation of dental implants, bone tissue recession, and alveolar process augmentation in adentia. In experiments on miniature pigs, we used the technology of two-stage splitting of the ridge of the alveolar process of the mandible in combination with a xenograft that was placed between the fragments of the split bone plate. The morphology of the reparative process and the distribution of osteogenic differentiation markers in the compact and trabecular bone of the alveolar crest were studied. Signs of reparative osteogenesis were observed in the bone regenerate that had a lamellar structure, formed osteons, and foci of woven tissue. It was found that the xenograft was replaced by newly formed trabecular bone tissue. These sites were characterized by increased expression of osteocalcin and CD44. Augmentation technology through two-stage splitting provides trophic relationship of osteoprogenitor cells and is an effective method of osteogenesis stimulation in the alveolar process.
Topics: Humans; Animals; Swine; Alveolar Ridge Augmentation; Osteogenesis; Bone Transplantation; Alveolar Process; Osseointegration; Mandible
PubMed: 38194070
DOI: 10.1007/s10517-024-06007-0