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Parasites, Hosts and Diseases Nov 2023Paleoparasitology is a discipline that applies existing conventional and molecular techniques to study parasites found in ancient ruins. This review focuses on the... (Review)
Review
Paleoparasitology is a discipline that applies existing conventional and molecular techniques to study parasites found in ancient ruins. This review focuses on the history of the discovery of parasites (mostly helminth eggs and larvae) in archaeological soil samples and mummies in Korea from the Three Kingdoms Period to the Joseon Dynasty (100 BCE-1910 CE). We also briefly review important milestones in global paleoparasitology. The helminth species reported so far in Korea included Ascaris lumbricoides, Trichuris trichiura, Strongyloides stercoralis (larva), Trichostrongylus sp. (larva), Paracapillaria philippinensis (syn. Capillaria philippinensis), Enterobius vermicularis, Fasciola hepatica, dicrocoeliids, Paragonimus westermani, Clonorchis sinensis, Metagonimus yokogawai, Pygidiopsis summa, Gymnophalloides seoi, Isthmiophora hortensis, Dibothriocephalus nihonkaiensis (syn. Diphyllobothrium nihonkaiense), and Taenia spp. tapeworms. The findings obtained by Korean paleoparasitologists/archaeologists have brought about deep insight into the status of helminthic infections in Korea's past populations. Continued paleoparasitological research is essential for further understanding of ancient parasites and parasitic diseases in Korea.
Topics: Animals; Larva; Republic of Korea; Parasitic Diseases; Helminthiasis; Trematoda; Parasites; Heterophyidae
PubMed: 38043533
DOI: 10.3347/PHD.23085 -
International Journal of Infectious... Aug 2023
Topics: Animals; Humans; Paragonimus westermani; Mediastinal Cyst; Paragonimiasis
PubMed: 37150353
DOI: 10.1016/j.ijid.2023.05.001 -
Journal of Clinical Microbiology Apr 2024Read the full article for the answer.
Read the full article for the answer.
PubMed: 38597632
DOI: 10.1128/jcm.01259-23 -
The American Journal of Tropical... Jul 2023Paragonimiasis is an important but neglected foodborne trematodiasis caused by Paragonimus mexicanus in Costa Rica. Immunological techniques for diagnosing this...
Paragonimiasis is an important but neglected foodborne trematodiasis caused by Paragonimus mexicanus in Costa Rica. Immunological techniques for diagnosing this parasitosis in humans do not exist in Central America. The objective of the present study was to use recombinant Paragonimus westermani cysteine protease 7 to standardize an ELISA for the detection of antibodies against Paragonimus spp. Human sera positive for P. westermani, P. mexicanus, or Paragonimus spp., human sera infected with other helminths, as well as sera of healthy humans without parasitic infections, were analyzed. The sensitivity of the ELISA was 92.9%, and the specificity was 91.9%. This report is the first to describe the development of an ELISA for the diagnosis of Paragonimus spp. in Costa Rica and Central America. Using this ELISA in the health system of Costa Rica is recommended to detect infections.
Topics: Animals; Humans; Paragonimiasis; Paragonimus westermani; Cysteine Proteases; Paragonimus; Enzyme-Linked Immunosorbent Assay; Costa Rica
PubMed: 37253437
DOI: 10.4269/ajtmh.22-0452 -
Pathology International Aug 2023
Topics: Animals; Humans; Paragonimus westermani; Brachyura; Soy Foods; Trematoda; Fresh Water
PubMed: 37493110
DOI: 10.1111/pin.13352 -
The American Journal of Tropical... Nov 2023
Topics: Humans; Animals; Paragonimiasis; Pleural Effusion; Paragonimus
PubMed: 37549895
DOI: 10.4269/ajtmh.23-0301 -
Tropical Medicine and Infectious Disease Dec 2023Human pulmonary paragonimiasis, an emerging concern in North East India, frequently masquerades as pulmonary tuberculosis due to clinical and radiological similarities,...
Human pulmonary paragonimiasis, an emerging concern in North East India, frequently masquerades as pulmonary tuberculosis due to clinical and radiological similarities, leading to diagnostic challenges. This research aimed to harness the immunoblotting technique to discern immunodiagnostic protein antigens from both adult worm and excretory-secretory (ES) extracts of the prevalent type 1 in Arunachal Pradesh, North East India. We studied the time kinetics of immunoreactive patterns in relation to the duration of infection in rodent models. Immunoblot analyses were also conducted using sera from ELISA-positive patients confirmed with paragonimiasis, facilitating the selection of antigenic extracts with diagnostic potential. Further, ES protein antigens were subjected to 2D immunoblot analysis and immunoreactive protein spots identified using MALDI-TOF MS. The immunoreactivity patterns of ES antigens with sera of paragonimiasis-positive patients were detailed, and specific immunoreactive protein antigens were pinpointed using peptide mass fingerprinting (MALDI-TOF). This work underscores the enhanced diagnostic accuracy when combining ELISA with immunoblotting for pulmonary paragonimiasis in regions like North East India, marked by co-existing helminth infections.
PubMed: 38251203
DOI: 10.3390/tropicalmed9010006 -
Zhongguo Xue Xi Chong Bing Fang Zhi Za... Mar 2024To investigate the population distribution of intermediate host snails and crabs of along the Jiulongjiang River, Zhangjiang River, and Dongxi River basins in...
[Investigation of the population diversity of intermediate host snails and crabs of along Jiulong River, Zhangjiang River and Dongxi River basins in southern Fujian Province].
OBJECTIVE
To investigate the population distribution of intermediate host snails and crabs of along the Jiulongjiang River, Zhangjiang River, and Dongxi River basins in Bopingling Mountain, southern Fujian Province, so as to provide baseline data for researches on parasitic disease prevention and control and enlargement of samples in the parasitic resource bank.
METHODS
A total of 23 villages in 8 counties (districts) along the Jiulong River, Zhangjiang River, and Dongxi River basins in Zhangzhou City, Fujian Province were selected as survey sites during the period from November 2020 through March 2023, and snail and freshwater crabs were sampled from 1 to 3 streams and ditches neighboring residential areas in each village. Morphological identification of snails was performed according to the external morphological characteristics of collected snail shells, and the unidentified snail species sampled from the natural foci of paragonimiasis in Yunxiao County were subjected to se-quence analysis of the mitochondrial cytochrome oxidase 1 () gene. The crab species was identified by observing the morphological characteristics of the terminal segment of the first pleopod of male crabs, and cercariae and metacercariae were detected in collected snails.
RESULTS
The shells of the unidentified snails sampled from the natural foci of paragonimiasis in Yunxiao County were approximately 50 mm in height and 18 mm in width, thick and solid, long tower cone-shaped, and had 8 to 10 whorls. gene sequence analysis identified the snail species as . A total of 6 freshwater snail species belonging to 5 genera within 3 families, identified 23 survey sites, including , and that belonged to the Family Pleurceridae, and that belonged to the subfamily Triculinae, Family Pomatiopsidae, and (Family Thiaridae), and 11 species of freshwater crabs belonging to 5 genera within 2 families were identified, including genus of , , and , genus of and , genus of and , and genus of and that belonged to the Family Potamidae, and genus of and (Family Parathelphusidae). In addition, the prevalence of cercariae infections was 0.08% (2/2 317) in from Danyan Village in Changtai District and 0.09% (1/1 039) in from Jinkeng Village in Yunxiao County, and the prevalence of metacercariae infections was 25.81% (8/31) in from Danyan Village in Changtai District, and 26.31% (5/19) in from Jinkeng Village in Yunxiao County, respectively.
CONCLUSIONS
There is a population diversity in the intermediate host snails and crabs along the Jiulongjiang River, Zhangjiang River, and Dongxi River basins in Bopingling Mountain, southern Fujian Province, and and are, for the first time, confirmed as the first intermediate hosts of .
Topics: Humans; Animals; Male; Paragonimus; Brachyura; Paragonimiasis; Rivers; Fresh Water; Gastropoda
PubMed: 38604684
DOI: 10.16250/j.32.1374.2023144 -
Clinical Nuclear Medicine Aug 2023A 32-year-old man underwent 18 F-FDG PET/CT to evaluate suspicious hepatic metastases, which were revealed by ultrasonography and MRI. The FDG PET/CT images demonstrated...
A 32-year-old man underwent 18 F-FDG PET/CT to evaluate suspicious hepatic metastases, which were revealed by ultrasonography and MRI. The FDG PET/CT images demonstrated only one focus of subtly increased activity in the liver without abnormality elsewhere. The pathological result from hepatic biopsy was consistent with Paragonimus westermani infection.
Topics: Humans; Male; Adult; Paragonimiasis; Liver; Positron Emission Tomography Computed Tomography; Magnetic Resonance Imaging; Ultrasonography
PubMed: 37290448
DOI: 10.1097/RLU.0000000000004736