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PeerJ 2024To study the efficacy of PAD Plus-based photoactivated disinfection (PAD) for treating denture stomatitis (DS) in diabetic rats by establishing a diabetic rat DS model.
OBJECTIVE
To study the efficacy of PAD Plus-based photoactivated disinfection (PAD) for treating denture stomatitis (DS) in diabetic rats by establishing a diabetic rat DS model.
METHODS
The diabetic rat DS model was developed by randomly selecting 2-month-old male Sprague-Dawley rats and dividing them into four groups. The palate and denture surfaces of rats in the PAD groups were incubated with 1 mg/mL toluidine blue O for 1 min each, followed by a 1-min exposure to 750-mW light-emitting diode light. The PAD-1 group received one radiation treatment, and the PAD-2 group received three radiation treatments over 5 days with a 1-day interval. The nystatin (NYS) group received treatment for 5 days with a suspension of NYS of 100,000 IU. The infection group did not receive any treatment. In each group, assessments included an inflammation score of the palate, tests for fungal load, histological evaluation, and immunohistochemical detection of interleukin-17 (IL-17) and tumor necrosis factor (TNF-α) conducted 1 and 7 days following the conclusion of treatment.
RESULTS
One day after treatment, the fungal load on the palate and dentures, as well as the mean optical density values of IL-17 and TNF-α, were found to be greater in the infection group than in the other three treatment groups ( < 0.05). On the 7th day after treatment, these values were significantly higher in the infection group than in the PAD-2 and NYS groups ( < 0.05). Importantly, there were no differences between the infection and PAD-1 groups nor between the PAD-2 and NYS groups ( 0.05).
CONCLUSIONS
PAD effectively reduced the fungal load and the expressions of IL-17 and TNF-α in the palate and denture of diabetic DS rats. The efficacy of multiple-light treatments was superior to that of single-light treatments and similar to that of NYS.
Topics: Animals; Male; Rats, Sprague-Dawley; Rats; Stomatitis, Denture; Disinfection; Diabetes Mellitus, Experimental; Tolonium Chloride; Tumor Necrosis Factor-alpha; Interleukin-17; Disease Models, Animal
PubMed: 38708351
DOI: 10.7717/peerj.17268 -
Journal of Analytical Toxicology Sep 2023Rilmazafone is a pro-drug that can be prescribed in Japan to treat insomnia. Rilmazafone metabolizes into active compounds by a ring closure resulting in a triazolo...
Rilmazafone is a pro-drug that can be prescribed in Japan to treat insomnia. Rilmazafone metabolizes into active compounds by a ring closure resulting in a triazolo benzodiazepine structure similar to alprazolam. In mid-2022, the National Board of Forensic Medicine in Sweden were requested to investigate two separate deaths with the suspected use of pagoclone. Packages labeled "Pagoclone" were found at each scene that was suspected to contain rilmazafone based on website information. During screening by high resolution mass spectrometry, rilmazafone metabolites were presumptively identified. Due to the lack of reference material for the active metabolites, the metabolites were synthesized in house and quantification of the compounds identified in the two autopsy cases was prompted. In Case 1, femoral blood concentrations of 7.9, 65 and 170 ng/g of the metabolites rilmazolam, N-desmethyl rilmazolam and di-desmethyl rilmazolam, respectively, were detected. Additional toxicological findings included the medications haloperidol, alimemazine, fluoxetine, olanzapine and acetaminophen. In Case 2, femoral blood concentrations of 1.7, 1.4 and 70 ng/g of rimazolam, N-desmethyl rilmazolam and di-desmethyl rilmazolam, respectively, were detected. Additional toxicological findings included loperamide, alimemazine and pregabalin. The intake of rilmazafone was determined as the cause of death in Case 1 and contributed in the Case 2.
Topics: Benzodiazepines; Prodrugs; Trimeprazine; Triazoles
PubMed: 37348041
DOI: 10.1093/jat/bkad041 -
Environmental Research Aug 2023In this study, the deployment of post Reverse Osmosis (RO)-carbon as a adsorbent for dye removal from water has been investigated. The post RO-carbon was thermally...
In this study, the deployment of post Reverse Osmosis (RO)-carbon as a adsorbent for dye removal from water has been investigated. The post RO-carbon was thermally activated (RO900), and the material thus obtained exhibited high surface area viz. 753 m/g. In the batch system, the efficient Methylene Blue (MB) and Methyl Orange (MO) removal was obtained by using 0.08 g and 0.13 g/50 mL adsorbent dosage respectively. Moreover, 420 min was the optimized equilibration time for both the dyes. The maximum adsorption capacities of RO900 for MB and MO dyes were 223.29 and 158.14 mg/g, respectively. The comparatively higher MB adsorption was attributed to the electrostatic attraction between adsorbent and MB. The thermodynamic findings revealed the process as spontaneous, endothermic, and accompanied by entropy increment. Additionally, simulated effluent was treated, and >99% dye removal efficiency was achieved. To mimic an industrial perspective, MB adsorption onto RO900 was also carried out in continuous mode. The initial dye concentration and effluent flow rate were among the process parameters that were optimized using the continuous mode of operation. Further, the experimental data of continuous mode was fitted with Clark, Yan, and Yoon-Nelson models. Py-GC/MS investigation revealed that dye-loaded adsorbents could be pyrolyzed to produce valuable chemicals. The cost and low toxicity associated benefits of discarded RO-carbon over other adsorbents reveal the significance of the present study.
Topics: Wastewater; Carbon; Coloring Agents; Adsorption; Water Pollutants, Chemical; Kinetics; Thermodynamics; Methylene Blue; Osmosis; Hydrogen-Ion Concentration
PubMed: 37196691
DOI: 10.1016/j.envres.2023.116165 -
New Zealand Veterinary Journal Sep 2023To evaluate the effect of IM administration of three sedative drugs, acepromazine, alfaxalone and dexmedetomidine, in combination with morphine, on the size of the...
AIMS
To evaluate the effect of IM administration of three sedative drugs, acepromazine, alfaxalone and dexmedetomidine, in combination with morphine, on the size of the feline spleen using ultrasonography.
METHODS
Twenty-four client-owned cats undergoing elective de-sexing or minor procedures were recruited for a focused ultrasonographic examination of the spleen prior to and at 10, 20 and 30 minutes following administration of one of three randomly assigned IM sedation protocols: 0.05 mg/kg acepromazine (ACE group), 3 mg/kg alfaxalone (ALF group), or 10 μg/kg dexmedetomidine (DEX group), in combination with 0.5 mg/kg morphine. B-mode images of the spleen were collected and measured following a standardised protocol. Cardiorespiratory parameters and sedation score were also recorded. Mean thickness of the head, body and tail of the spleen for each group at 10, 20 and 30 minutes after drug administration was compared to baseline.
RESULTS
Mean splenic thickness increased over time in the ACE group (thickness of body at T0 = 8.9 (SE 2.1) mm and at T30 = 10.5 (SE 2.0) mm; p = 0.001) and the ALF group (thickness of body at T0 = 8.8 (SE 1.0) mm and at T30 = 10.3 (SE 1.7) mm; p = 0.022) but not in the DEX group (thickness of body at T0 = 8.6 mm (1.2) and at T30 = 8.9 mm (0.6); p = 0.67). Mean arterial blood pressure in the DEX group was significantly higher than in the other groups (p = 0.002). Sedation scores in the DEX group were consistently high for the entire period. However, the sedation score in the ACE group increased over 30 minutes (p = 0.007). Sedation score in the ALF group was highest at 10 minutes but gradually decreased over the following 20 minutes (p = 0.003).
CONCLUSIONS
Sedation with IM dexmedetomidine and morphine did not change splenic size, whereas acepromazine or alfaxalone and morphine increased it regardless of the degree of sedation.
CLINICAL RELEVANCE
Where splenomegaly is identified in a cat sedated with acepromazine or alfaxalone, the effects of the sedation protocol could be considered as a possible cause.
Topics: Cats; Animals; Dexmedetomidine; Acepromazine; Spleen; Hypnotics and Sedatives; Morphine; Ultrasonography
PubMed: 37345417
DOI: 10.1080/00480169.2023.2223177 -
Journal of Environmental Management Jan 2024In this study, a new porous nanocomposite adsorbent for water conservancy was synthesized using the freeze-drying technique to adsorb a cationic dye (Methylene Blue) in...
Synthesis and characterization of Alg/Gel/n-HAP/MNPs porous nanocomposite adsorbent for efficient water conservancy and removal of methylene blue in aqueous environments: Kinetic modeling and artificial neural network predictions.
In this study, a new porous nanocomposite adsorbent for water conservancy was synthesized using the freeze-drying technique to adsorb a cationic dye (Methylene Blue) in an aqueous environment. The nanocomposite adsorbent was synthesized using natural polymers, gelatin, and sodium alginate, and hydroxyapatite and magnetic iron oxide nanoparticles was incorporated into the polymer network to improve mechanical properties and increase the surface-to-volume ratio. To confirm the structure and morphology of the sample, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and scanning electron microscope (SEM) techniques were employed. In addition, the magnetic properties of the synthesis of MNPs and porous nanocomposite were determined using value stream mapping (VSM) and dynamic light scattering (DLS). The adsorption of Methylene Blue (MB) was studied as a function of effective physical and variable parameters, such as time, temperature, pH, and initial concentration. The synthesized porous nanocomposite adsorbent exhibited a high adsorption capacity of 473.2 mg g and followed pseudo-second-order kinetics. Additionally, the maximum adsorption capacity was observed at an initial concentration of 534.9 mg g. The adsorbent was also sensitive to temperature changes and was well-described thermodynamically and isothermally by the Freundlich isotherm model. Two artificial neural networks (ANNs) were also developed to investigate the properties of the synthesized nanocomposites. In the first ANN, the properties of the nanocomposites, including pore size, porosity, compressive strength, and elastic modulus, were predicted based on the variations in the weight percentages of gelatin and hydroxyapatite. In the second ANN, the effects of changes in temperature and initial concentration on the adsorption of MB by the synthesized nanocomposite samples were predicted. The ANNs' predictions indicated that increasing the weight percentage of hydroxyapatite nanoparticles and gelatin enhances the physical, mechanical, and adsorption performance of the synthesized porous nanocomposites. The best results were achieved for the sample containing 40 wt % of gelatin and 30 wt % of hydroxyapatite nanoparticles. Furthermore, the ANN models demonstrated that increasing the temperature and initial concentration resulted in an increase in the amount of MB adsorbed.
Topics: Water; Methylene Blue; Porosity; Gelatin; Neural Networks, Computer; Nanocomposites; Adsorption; Hydroxyapatites; Water Pollutants, Chemical; Kinetics; Hydrogen-Ion Concentration
PubMed: 37918240
DOI: 10.1016/j.jenvman.2023.119446 -
Environmental Pollution (Barking, Essex... Jul 2023Understanding the spatial and temporal penetration patterns of organic pollutants in microplastics (μP) is important for evaluating their environmental and biological...
Understanding the spatial and temporal penetration patterns of organic pollutants in microplastics (μP) is important for evaluating their environmental and biological impacts, such as the "Trojan Horse" effect. However, there is a lack of an effective method to monitor the penetration processes and patterns in situ. This study aimed to develop a simple and sensitive approach for in situ imaging of organic pollutant penetration into μP. The novel method was developed using surface-enhanced Raman spectroscopy (SERS) coupled with gold nanoparticles as nanoprobes that could sensitively detect organic pollutants in low-density polyethylene (LDPE) μP spatially and temporally. The detection limit of this SERS-based method was 0.36 and 0.02 ng/mm for ferbam (pesticide) and methylene blue (synthetic dye), respectively. The results showed that both ferbam and methylene blue could penetrate LDPE μP. The penetration depth and amount increased as the interaction time increased. Most of the absorbed organic pollutants accumulated within the top 90 μm layer of the tested μP. Compared to methylene blue, ferbam was more quickly absorbed and achieved higher accumulation in μP with a maximum of 32.57 ng/mm after 168 h interaction. This pioneering study clearly demonstrated that SERS mapping is a sensitive and in situ approach to visualize and quantify the penetration patterns of organic pollutants in μP. The new approach developed here can advance our understanding of μP as pollutant carriers and their influence on the environmental fate, behavior, and biological impacts of organic pollutants.
Topics: Spectrum Analysis, Raman; Metal Nanoparticles; Microplastics; Plastics; Environmental Pollutants; Gold; Polyethylene; Methylene Blue
PubMed: 37098368
DOI: 10.1016/j.envpol.2023.121712 -
Chemosphere Oct 2023Dyestuff wastewater and pharmaceutical wastewater have become typical representatives of water pollution. In this study, a novel nano-silica-biochar composite (NSBC) was...
Dyestuff wastewater and pharmaceutical wastewater have become typical representatives of water pollution. In this study, a novel nano-silica-biochar composite (NSBC) was synthesized based on corn straw as raw material, by a combination of ball milling, pyrolysis and KOH activation. The modified biochar with rough surface had higher specific surface area (117.67-132.82 m/g), developed pore structure (0.12-0.15 cm/g) and abundant surface functional groups (-OH, -COOH, Si-O and aromatic CC were dominated). These provided abundant active sites for the adsorption of pollutants. The adsorption capacities of NSBC for Methylene Blue (MB) and Tetracycline (TC) were both higher than that of other similar products, the maximum adsorption capacity of Langmuir were 247.22 and 86.95 mg/g, respectively. After five adsorption-desorption cycle experiments, the adsorption capacities of NSBC for both were still excellent, reaching 99.30 and 19.87 mg/g, respectively. Due to the different structure and molecular size of MB and TC, the adsorption capacities of NSBC were significantly different, especially the influence of solution pH value. The adsorption mechanisms were comprehensively discussed by FTIR and XPS of the samples before and after adsorption, and combining experimental results of BET and simultaneously, which were manifested as monolayer chemisorption, specifically surface complexation, hydrogen bonding, n-π/π-π conjugation, electrostatic interaction and pore filling.
Topics: Methylene Blue; Silicon Dioxide; Wastewater; Adsorption; Charcoal; Anti-Bacterial Agents; Tetracycline; Water Pollutants, Chemical; Kinetics
PubMed: 37399993
DOI: 10.1016/j.chemosphere.2023.139395 -
Chemosphere Oct 2023The presence of dyes in contaminated water poses substantial dangers to the health of both humans and aquatic life. A process called precipitation polymerization was...
The presence of dyes in contaminated water poses substantial dangers to the health of both humans and aquatic life. A process called precipitation polymerization was used to create unique cross-linked hexa-chlorocyclotriphosphazene-co-phenolphthalein (Hex-CCP-co-PPT) microspheres for the purpose of this research. Advanced methods such as X-ray diffraction (XRD), energy-dispersive X-ray spectroscopy (EDX), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and differential thermogravimetry (DTG) were used to characterise these microspheres. In a simulated solution, the performance of Hex-CCP-co-PPTs as a sorbent for removing MB dye was investigated, and the results showed an unprecedentedly high removal rate of 88.4% for MB. Temperature of 25 °C, a Hex-CCP-co-PPTs dose of 40 mg, an MB concentration of 20 ppm, an MB solution volume of 20 mL, a contact time of 40 min, and a pH of 9 were found to be the optimal experimental conditions. According to the results of the kinetic and adsorption analyses, the PSO and Langmuir adsorption models are the best ones to use. These models favour the chemi-sorption nature and mono-layered adsorption of MB in comparison to Hex-CCP-co-PPTs. Importantly, the thermodynamic analysis demonstrated that the process of removing MB by utilizing Hex-CCP-co-PPTs was endothermic and occurred spontaneously. These findings highlight the potential application of Hex-CCP-co-PPT microspheres in Algal Membrane Bioreactors (AMBRs) for the efficient and sustainable removal of dye from wastewater. This would contribute to the protection of ecosystems as well as the public's health.
Topics: Humans; Wastewater; Coloring Agents; Microspheres; Ecosystem; Methylene Blue; Water Pollutants, Chemical; Thermodynamics; Adsorption; Kinetics; Hydrogen-Ion Concentration; Spectroscopy, Fourier Transform Infrared
PubMed: 37499806
DOI: 10.1016/j.chemosphere.2023.139637 -
Environmental Research Jul 2023The complexity of the chemistry behind the hydrothermal conversion is enormous. Components interact with their own physical and chemical structure, making it harsh to...
The complexity of the chemistry behind the hydrothermal conversion is enormous. Components interact with their own physical and chemical structure, making it harsh to understand the conversion as a whole. Herein, the six-water recirculation and loading nano SiO experiment in a one-pot hydrothermal carbonization procedure was designed to elucidate the mechanism of regulating the functional groups and microporous structure of the hydrochar surface. The hydrochar prepared by the second circulating liquid and loading nano-SiO (HBC-R2/Si) was equipped most enriched functional groups (carboxyl = 11.48 μmol/g, phenolic hydroxyl = 52.98 μmol/g, lactone groups = 46.52 μmol/g) and suitable pore size (1.90 nm-1.93 nm) as a sorbent riched in hemicellulose. The sorption kinetics (equilibrium reached ≈ 480 min) are approximately evenly fitted by the pseudo-second-order, Weber and Morris, and Elovich models, indicating that membranes and particles diffusion, pore diffusion, and surface sorption coexisted in the sorption of methylene blue (MB) on the hydrochar materials. Simultaneously, all hydrochar materials achieved over 25% MB removal within 90 min (liquid membrane diffusion) and over 40% for HBC-R2 and HBC-R2/Si, suggesting that liquid membrane diffusion is the predominant rate-limiting step. Pearson's correlation analysis and Mantel's analysis announced that the cation exchange capacity (CEC), pore size, and carboxyl groups on the hemicellulose affect the sorption capacity by limiting the pore diffusion procedure. However, the CEC and the phenolic hydroxyl groups on the cellulose and hemicellulose affect the sorption rate by limiting membrane diffusion. Three consecutive sorption/desorption cycles confirmed the high stability and reusability of HBC-R2/Si composites.
Topics: Carbon; Cellulose; Kinetics; Methylene Blue; Surface Properties; Adsorption
PubMed: 37127106
DOI: 10.1016/j.envres.2023.116003 -
Journal of Materials Chemistry. B Nov 2023We have developed a targeted nano-drug delivery system that effectively harnesses the anti-tumor properties of trifluoperazine (TFP), while concurrently mitigating its...
We have developed a targeted nano-drug delivery system that effectively harnesses the anti-tumor properties of trifluoperazine (TFP), while concurrently mitigating its side effects on the central nervous system. The manufacturing process entailed the preparation of mesoporous silica nanoparticles (MSN-NH), followed by the loading of trifluoperazine into the pores of MSN-NH and then surface modification with polyethylene glycol (PEG) and anisamide (AA), resulting in the formation of TFP@MSN@PEG-AA (abbreviated as TMPA) nanoparticles. and anti-tumor activity and hemolysis experiments showed that TMPA had an excellent safety profile and a good anti-tumor effect. Importantly, the drug content of the TMPA nanoparticle group was found to be significantly lower than that of the TFP group in the mouse brain tissue as determined by High Performance Liquid Chromatography (HPLC) detection. Therefore, the developed drug delivery system achieved the goal of maintaining TFP's anti-tumor action while avoiding its negative effects on the central nervous system.
Topics: Mice; Animals; Silicon Dioxide; Trifluoperazine; Drug Delivery Systems; Nanoparticles; Neoplasms
PubMed: 37876312
DOI: 10.1039/d3tb01472j