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Biomaterials Research Dec 2023Magnesium oxychloride cement has good mechanical properties, but poor water resistance.
BACKGROUND
Magnesium oxychloride cement has good mechanical properties, but poor water resistance.
METHODS
Phytic acid, which can form chelate with Mg, was used to modify magnesium oxychloride cement, and the effects of phytic acid on the strength, in vitro degradation and biological activity of magnesium oxychloride cement were studied. Based on the preparation of phytic acid modified magnesium oxychloride cement with good water resistance and biological activity, osteoporosis treatment strontium ranelate was loaded on phytic acid- magnesium oxychloride cement, strontium ranelate/phytic acid-magnesium oxychloride cement was prepared.
RESULTS
It was found that the compressive strength of 1.25 wt% phytic acid-magnesium oxychloride cement after soaking in SBF for 28 d could reach 40.5 ± 2.0 MPa, 13.33% higher than that of the control group (when phytic acid was 0 wt%), and the mass loss rate of all ages was lower than that of the control group. The water resistance of magnesium oxychloride cement was effectively improved by phytic acid. After loading with strontium ranelate, the water resistance of 1.25 wt% phytic acid-magnesium oxychloride cement was improved. Cell experiments showed that strontium ranelate could effectively promote cell proliferation and improve the expression of osteoblast-related proteins. When strontium ranelate/phytic acid-magnesium oxychloride cement samples were implanted subcutaneously in rats for 4 w, no obvious inflammatory response was observed, and the material was tightly bound to the surrounding tissues. When bone cement was implanted into rat femur for 4 w, the bone cement was gradually wrapped and absorbed by new bone tissue, which grew from the outside to the inside, indicating that the bone cement containing strontium ranelate/phytic acid-magnesium oxychloride cement had excellent bone-forming ability.
CONCLUSIONS
In conclusion, the results indicated that strontium ranelate/phytic acid-magnesium oxychloride cement composite bone cement had a potential application prospect in clinical bone repair.
PubMed: 38072982
DOI: 10.1186/s40824-023-00474-8 -
Journal of Materials Science. Materials... Jun 2024Calcium phosphate cements, primarily brushite cements, require the addition of setting retarders to ensure adequate processing time and processability. So far, citric...
Calcium phosphate cements, primarily brushite cements, require the addition of setting retarders to ensure adequate processing time and processability. So far, citric acid has been the primary setting retarder used in this context. Due to the poor biocompatibility, it is crucial to explore alternative options for better processing. In recent years, the setting retarder phytic acid (IP6) has been increasingly investigated. This study investigates the biological behaviour of calcium phosphate cements with varying concentrations of IP6, in addition to their physical properties. Therefore cytocompatibility in vitro testing was performed using osteoblastic (MG-63) and osteoclastic (RAW 264.7 differentiated with RANKL) cells. We could demonstrate that the physical properties like the compressive strength of specimens formed with IP6 (brushite_IP6_5 = 11.2 MPa) were improved compared to the reference (brushite = 9.8 MPa). In osteoblast and osteoclast assays, IP6 exhibited significantly better cytocompatibility in terms of cell activity and cell number for brushite cements up to 11 times compared to the brushite reference. In contrast, the calcium-deficient hydroxyapatite (CDHA) cements produced similar results for IP6 (CDHA_IP6_0.25 = 27.0 MPa) when compared to their reference (CDHA = 21.2 MPa). Interestingly, lower doses of IP6 were found to be more effective than higher doses with up to 3 times higher. Additionally, IP6 significantly increased degradation in both passive and active resorption. For these reasons, IP6 is emerging as a strong new competitor to established setting retarders such as citric acid. These cements have potential applications in bone augmentation, the stabilisation of non-load bearing fractures (craniofacial), or the cementation of metal implants.
Topics: Phytic Acid; Animals; Calcium Phosphates; Mice; Materials Testing; Bone Cements; Osteoblasts; RAW 264.7 Cells; Humans; Osteoclasts; Compressive Strength; Biocompatible Materials; Durapatite
PubMed: 38900219
DOI: 10.1007/s10856-024-06805-y -
Carbohydrate Polymers Sep 2024Efficient removal of uranium from radioactive wastewater is crucial for both environmental protection and sustainable development of nuclear energy. However, selectively...
Efficient removal of uranium from radioactive wastewater is crucial for both environmental protection and sustainable development of nuclear energy. However, selectively extracting uranium from acidic wastewater remains a significant challenge. Here we present a phytic acid-functionalized polyamidoxime/alginate hydrogel (PAG) via a facile one-step hydrothermal reaction. The PAG, leveraging the robust binding affinity of phytic acid and the selective coordination of amidoxime for U(VI), exhibited high efficiency and selectivity in adsorbing U(VI) from acidic uranium-containing wastewater. At pH 2.50, U(VI) adsorption equilibrium was achieved within 60 min, showcasing a maximum theoretical adsorption capacity of 218.34 mg/g. Additionally, the PAG demonstrated excellent reusability, maintaining a uranium removal rate exceeding 90 % over five adsorption-desorption cycles. Remarkably, the as-synthesized PAG removed 94.1 % of U(VI) from actual acidic uranium-contaminated groundwater with excellent anti-interference performance, reducing U(VI) concentration from 272.0 μg/L to 16.1 μg/L and making it meet the WHO drinking water standards (30 μg/L). The adsorption mechanism was elucidated through XPS and DFT calculation, revealing that the uranyl ion primarily coordinated with phosphate and amidoxime groups on phytic acid and polyamidoxime, respectively. These findings underscore the promising potential of PAG hydrogel for addressing acidic uranium-containing wastewater from uranium mining and metallurgy.
PubMed: 38823934
DOI: 10.1016/j.carbpol.2024.122283 -
World Journal of Microbiology &... Nov 2023Phytases are important enzymes used for eliminating the anti-nutritional properties of phytic acid in food and feed ingredients. Phytic acid is major form of organic... (Review)
Review
Phytases are important enzymes used for eliminating the anti-nutritional properties of phytic acid in food and feed ingredients. Phytic acid is major form of organic phosphorus stored during seed setting. Monogastric animals cannot utilize this phytate-phosphorus due to lack of necessary enzymes. Therefore, phytic acid excretion is responsible for mineral deficiency and phosphorus pollution. Phytases have been reported from diverse microorganisms, however, fungal phytases are preferred due to their unique properties. Aspergillus species are the predominant producers of phytases and have been explored widely as compared to other fungi. Solid-state fermentation has been studied as an economical process for the production of phytases to utilize various agro-industrial residues. Mixed substrate fermentation has also been reported for the production of phytases. Physical and chemical parameters including pH, temperature, and concentrations of media components have significantly affected the production of phytases in solid state fermentation. Fungi produced high levels of phytases in solid state fermentation utilizing economical substrates. Optimization of culture conditions using different approaches has significantly improved the production of phytases. Fungal phytases are histidine acid phosphatases exhibiting broad substrate specificity, are relatively thermostable and protease-resistant. These phytases have been found effective in dephytinization of food and feed samples with concomitant liberation of minerals, sugars and soluble proteins. Additionally, they have improved the growth of plants by increasing the availability of phosphorus and other minerals. Furthermore, phytases from fungi have played an important roles in bread making, semi-synthesis of peroxidase, biofuel production, production of myo-inositol phosphates and management of environmental pollution. This review article describes the production of fungal phytases in solid state fermentation and their biotechnological applications.
Topics: Animals; 6-Phytase; Fermentation; Phytic Acid; Phosphorus; Minerals
PubMed: 38008864
DOI: 10.1007/s11274-023-03783-1 -
ACS Applied Bio Materials May 2024Medical implants are constantly facing the risk of bacterial infections, especially infections caused by multidrug resistant bacteria. To mitigate this problem, gold...
Phytic Acid-Promoted Deposition of Gold Nanoparticles with Grafted Cationic Polymer Brushes for the Construction of Synergistic Contact-Killing and Photothermal Bactericidal Coatings.
Medical implants are constantly facing the risk of bacterial infections, especially infections caused by multidrug resistant bacteria. To mitigate this problem, gold nanoparticles with alkyl bromide moieties (Au NPs-Br) on the surfaces were prepared. Xenon light irradiation triggered the plasmon effect of Au NPs-Br to induce free radical graft polymerization of 2-(dimethylamino)ethyl methacrylate (DMAEMA), leading to the formation of poly(DMAEMA) brush-grafted Au NPs (Au NPs--PDM). The Au NPs--PDM nanocomposites were conjugated with phytic acid (PA) via electrostatic interaction and van der Waals interaction. The as-formed aggregates were deposited on the titanium (Ti) substrates to form the PA/Au NPs--PDM (PAP) hybrid coatings through surface adherence of PA and the gravitational effect. Synergistic bactericidal effects of contact-killing caused by the cationic PDM brushes, and local heating generated by the Au NPs under near-infrared irradiation, conferred strong antibacterial effects on the PAP-deposited Ti (Ti-PAP) substrates. The synergistic bactericidal effects reduced the threshold temperature required for the photothermal sterilization, which in turn minimized the secondary damage to the implant site. The Ti-PAP substrates exhibited 97.34% and 99.97% antibacterial and antiadhesive efficacy, respectively, against () and (), compared to the control under antimicrobial assays. Furthermore, the as-constructed Ti-PAP surface exhibited a 99.42% reduction in the inoculated under assays. In addition, the PAP coatings exhibited good biocompatibility in the hemolysis and cytotoxicity assays as well as in the subcutaneous implantation of rats.
Topics: Gold; Anti-Bacterial Agents; Metal Nanoparticles; Phytic Acid; Materials Testing; Staphylococcus aureus; Escherichia coli; Microbial Sensitivity Tests; Particle Size; Animals; Surface Properties; Coated Materials, Biocompatible; Cations; Polymers; Titanium
PubMed: 38727030
DOI: 10.1021/acsabm.4c00237 -
Soft Matter Jan 2024Conductive hydrogels have been widely researched for their potential applications in soft electronic devices. Creating environmentally friendly and multifunctional...
Conductive hydrogels have been widely researched for their potential applications in soft electronic devices. Creating environmentally friendly and multifunctional high-strength hydrogels for high-performance devices remains a significant challenge. This study employs the biodegradable material polyvinyl alcohol (PVA) as the primary component, with phytic acid (PA) and tannic acid (TA) as reinforcing phases, to create a multifunctional, high-strength "green" hydrogel. Through the multiple complexations of two bio-enhancing phases with the PVA main chain, this hydrogel attains ultra-high tensile strength (9.341 MPa), substantial toughness (4.262 MJ m), and extensive fracture strain (> 1000%), making it a representative with both mechanical performance and antibacterial capabilities. Additionally, it exhibits a low strain sensing limit (0.5%) and excellent durability (500 cycles under 50% strain). This work introduces a novel strategy of combining biodegradable materials with biomass to fabricate multifunctional hydrogels suitable for human motion monitoring and 2D pressure distribution.
Topics: Humans; Phytic Acid; Anti-Bacterial Agents; Electric Conductivity; Hydrogels; Polyvinyl Alcohol; Polyphenols
PubMed: 38164001
DOI: 10.1039/d3sm01295f -
Nucleic Acids Research Nov 2023The classical Non-Homologous End Joining (c-NHEJ) pathway is the predominant process in mammals for repairing endogenous, accidental or programmed DNA Double-Strand...
The classical Non-Homologous End Joining (c-NHEJ) pathway is the predominant process in mammals for repairing endogenous, accidental or programmed DNA Double-Strand Breaks. c-NHEJ is regulated by several accessory factors, post-translational modifications, endogenous chemical agents and metabolites. The metabolite inositol-hexaphosphate (IP6) stimulates c-NHEJ by interacting with the Ku70-Ku80 heterodimer (Ku). We report cryo-EM structures of apo- and DNA-bound Ku in complex with IP6, at 3.5 Å and 2.74 Å resolutions respectively, and an X-ray crystallography structure of a Ku in complex with DNA and IP6 at 3.7 Å. The Ku-IP6 interaction is mediated predominantly via salt bridges at the interface of the Ku70 and Ku80 subunits. This interaction is distant from the DNA, DNA-PKcs, APLF and PAXX binding sites and in close proximity to XLF binding site. Biophysical experiments show that IP6 binding increases the thermal stability of Ku by 2°C in a DNA-dependent manner, stabilizes Ku on DNA and enhances XLF affinity for Ku. In cells, selected mutagenesis of the IP6 binding pocket reduces both Ku accrual at damaged sites and XLF enrolment in the NHEJ complex, which translate into a lower end-joining efficiency. Thus, this study defines the molecular bases of the IP6 metabolite stimulatory effect on the c-NHEJ repair activity.
Topics: Animals; DNA; DNA Breaks, Double-Stranded; DNA End-Joining Repair; DNA-Binding Proteins; Ku Autoantigen; Mammals; Phytic Acid; Humans
PubMed: 37870477
DOI: 10.1093/nar/gkad863 -
Food Chemistry Jan 2024Corn starch dispersions were heated with ethanol (E) and reacted with phytic acid (E-PA), citric acid (E-CA), and a mixture of phytic and citric acid (E-PACA) under...
Corn starch dispersions were heated with ethanol (E) and reacted with phytic acid (E-PA), citric acid (E-CA), and a mixture of phytic and citric acid (E-PACA) under dry-heating to prepare heavy metal adsorbents. Microscopy images indicated that ethanol treatment induced the formation of porous structures on the surface; furthermore, treatment with phytic and citric acid induced indentations, pores, and irregular structures in E-PA, E-CA, and E-PACA starches. Phytic and citric acid were retained in the starch molecules through ester bonds with the phosphate and carboxyl groups, respectively. Starch esterification by phytic and citric acid induced a loss of crystallinity, high water absorption capacity, and low solubility. E-PACA starch exhibited more efficient Cu adsorption (38.13 mg/g) than native, E, E-PA, and E-CA starches (0.11, 0.49, 2.05, and 36.23 mg/g, respectively). Thus, modification with ethanol, phytic acid and citric acid can be applied to prepare natural starch-based heavy metal adsorbents.
Topics: Adsorption; Phytic Acid; Ethanol; Starch; Zea mays; Citric Acid; Metals, Heavy
PubMed: 37604005
DOI: 10.1016/j.foodchem.2023.137167 -
ACS Applied Materials & Interfaces Sep 2023Polyaniline (PANi) hydrogels that combine advantages of hydrogels and conductive PANi have recently emerged in areas of wearable devices and personal healthcare....
Polyaniline (PANi) hydrogels that combine advantages of hydrogels and conductive PANi have recently emerged in areas of wearable devices and personal healthcare. Nevertheless, their mechanical performance often gradually degrades after being used for a period, caused by destruction of the inner structures when external forces are applied. Inspired by biological structures with persistent durability, we develop here a phytic acid-assisted PANi/poly(vinyl alcohol) (PVA) hydrogel that shows self-reinforcing features. As a natural product holding plenty of phosphate groups, phytic acid (PA) plays two crucial roles when preparing this hydrogel: (1) aniline is salinized by PA in aqueous solution to promote in situ polymerization, making the resulting PANi conductive; (2) PA/PANi particles form hydrogen bonds with PVA, acting as stress concentration points to induce structure orientation. The optimal PVA/PA/PANi hydrogel displays dark green color with a uniform distribution of PA/PANi particles. After experiencing repetitive 4 × 100 stretching at a strain of 10%, the hydrogel exhibits an enhanced fracture strength (20.35 MPa), Young's modulus (22.66 MPa), and toughness (36.24 MJ·m) compared with the original hydrogel. This self-reinforcing feature is mainly attributed to the formation of anisotropic structures fixed by hydrogen bonds between PA/PANi particles and PVA chains upon repetitive external forces. Moreover, anisotropic structures can be disassembled by swelling the post-stretched hydrogel in water, and the swollen hydrogel shows similar self-reinforcing behaviors. The good mechanical durability and reusable characteristics make the PVA/PA/PANi hydrogel a reliable strain sensor. This work provides a structural growing-reviving approach for conductive hydrogels with persistent durability.
PubMed: 37615547
DOI: 10.1021/acsami.3c09032 -
British Poultry Science Dec 20231. Phytic acid (PA) is an antinutritional factor in poultry diets. The effect of high dietary PA in chicken diets might be exacerbated when the particle size of oyster...
1. Phytic acid (PA) is an antinutritional factor in poultry diets. The effect of high dietary PA in chicken diets might be exacerbated when the particle size of oyster shell (OS) is too fine. Thus, this study investigated the hypothesis that high PA with fine OS particle size would impair growth in broilers.2. Two hundred and eighty Cobb 500 broilers were assigned to four diets in a 2 × 2 factorial arrangement in a CRD. The factors were PA (low or high) and OS particle size (fine or coarse) in starter, grower and finisher diets. Data collected were performance, Ca digestibility, gastrointestinal pH and bone traits.3. On d 21, high PA increased intake ( < 0.05), gain ( = 0.099) and body weight (BW; = 0.093) compared to low PA. On d 42, high PA increased BW ( = 0.086) and gain ( = 0.089) compared to low PA. High PA increased intake ( = 0.063), BW ( = 0.054) and gain ( = 0.056) compared to low PA on d 56. High PA improved liveability on d 56 ( < 0.05) compared to low PA. In birds fed coarse OS, crop and ileal pH were reduced ( < 0.05) by high PA on d 28. The OS × PA interaction was observed for ileal pH ( < 0.05) on d 56, where in birds fed coarse OS, low PA increased ileal pH. Fine OS increased crop ( = 0.056) and proventriculus pH ( < 0.05) on d 56. There were no treatment effects on calcium digestibility. In birds fed fine OS, high PA decreased the BS ( < 0.05).4. Overall, the study showed that a combination of high PA and coarse OS particle size improves the production performance of broilers, while low PA and coarse OS improve their bone health.
Topics: Animals; Chickens; Calcium; Phytic Acid; Particle Size; Digestion; Animal Feed; Animal Nutritional Physiological Phenomena; Calcium Carbonate; Calcium, Dietary; Diet; Hydrogen-Ion Concentration; Ostreidae
PubMed: 37747291
DOI: 10.1080/00071668.2023.2262401