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Molecules (Basel, Switzerland) Aug 2023is a singular halophyte that has been shown to have anti-inflammatory, antioxidant, and allelopathic properties. In the work presented herein, we have characterized its...
is a singular halophyte that has been shown to have anti-inflammatory, antioxidant, and allelopathic properties. In the work presented herein, we have characterized its inflorescences hydromethanolic extract and assessed its antifungal activity for the pre- and postharvest management of tomato crop diseases. Gas chromatography-mass spectrometry characterization of the extract showed that 4-ethenyl-1,3-benzenediol, 2,3-dihydro-benzofuran, quinic acid, 3,5-dihydroxy-6,7,8-trimethoxy-2-phenyl-4H-1-benzopyran-4-one, 1,6-anhydro--D-glucopyranose, catechol, scopoletin, and maltol were the main constituents. The co-occurrence of pyranones, benzenediols, and quinic acids as phytoconstituents of extract resulted in promising in vitro minimum inhibitory concentrations of 500, 375, 500, 187.5, 187.5, and 375 μg·mL against mycelia of , f. sp. , and , respectively. Further, to assess the potential of inflorescence extract for postharvest tomato crop protection, ex situ tests were conducted against , obtaining high protection at a dose of 750 μg·mL. Taking into consideration that the demonstrated activity is among the highest reported to date for plant extracts and comparable to that of the synthetic fungicides tested as positive controls, inflorescence extract may be put forward as a promising biorational and may deserve further testing in field-scale studies.
Topics: Helichrysum; Inflorescence; Solanum lycopersicum; Plant Extracts; Fusarium; Disease Management; Plant Diseases
PubMed: 37570830
DOI: 10.3390/molecules28155861 -
Acta Chimica Slovenica Dec 2023In 2020, around 19.3 million new cancer cases resulted in 3.5 million deaths. It is estimated that there will be 28.4 million cases by 2040. Plant-derived natural...
In 2020, around 19.3 million new cancer cases resulted in 3.5 million deaths. It is estimated that there will be 28.4 million cases by 2040. Plant-derived natural products, such as alkaloids, flavonoids, terpenoids, and phenolic compounds, offer a wide range of chemical structures and biological activities that can be explored for their potential in cancer research. In a particular study, researchers investigated the potential anticancer effects of Fallopia flower extracts on cancer induced by TAA (a carcinogen). They examined the extracts to identify specific amino acids with antioxidant properties and assessed their impact on liver function and tissue structure. The results showed that the plant extracts had positive effects on the histological section of the TAA-treated group. Additionally, the group of male rats treated with TAA and a chemotherapy drug called MMC displayed positive effects. Similarly, the group of male rats treated with TAA, flower plant extract, and MMC also showed positive effects. These findings suggest that the extract from Fallopia flowers could be considered as a traditional therapy with potential anticancer properties.
Topics: Animals; Rats; Antioxidants; Carcinoma, Hepatocellular; Flavonoids; Liver Neoplasms; Plant Extracts; Terpenes; Polyphenols; Alkaloids
PubMed: 38124642
DOI: 10.17344/acsi.2023.8387 -
Critical Reviews in Biotechnology Jun 2024Among the many different types of wound dressings, nanofiber-based materials produced through electrospinning are claimed to be ideal because of their advantageous... (Review)
Review
Among the many different types of wound dressings, nanofiber-based materials produced through electrospinning are claimed to be ideal because of their advantageous intrinsic properties and the feasibility of employing several strategies to load bioactive compounds into their structure. Bioactive compounds with antimicrobial properties have been incorporated into different wound dressings to promote healing as well as prevent and treat bacterial infections. Among these, natural products, such as medicinal plant extracts and essential oils (EOs), have proven particularly attractive thanks to their nontoxic nature, minor side effects, desirable bioactive properties, and favorable effects on the healing process. To this end, the present review provides an exhaustive and up-to-date revision of the most prominent medicinal plant extracts and EOs with antimicrobial properties that have been incorporated into nanofiber-based wound dressings. The most common methods used for incorporating bioactive compounds into electrospun nanofibers include: pre-electrospinning (blend, encapsulation, coaxial, and emulsion electrospinning), post-electrospinning (physical adsorption, chemical immobilization, and layer-by-layer assembly), and nanoparticle loading. Furthermore, a general overview of the benefits of EOs and medicinal plant extracts is presented, describing their intrinsic properties and biotechniques for their incorporation into wound dressings. Finally, the current challenges and safety issues that need to be adequately clarified and addressed are discussed.
Topics: Oils, Volatile; Bandages; Anti-Bacterial Agents; Plant Extracts; Humans; Nanofibers; Wound Healing; Animals
PubMed: 37156536
DOI: 10.1080/07388551.2023.2193859 -
Cell Biochemistry and Function Dec 2023Piper chaba (Piperaceae) is a medicinal spice plant that possesses several pharmacological activities. In the present study, we for the first time studied the effect of...
Piper chaba (Piperaceae) is a medicinal spice plant that possesses several pharmacological activities. In the present study, we for the first time studied the effect of P. chaba extract on breast cancer cells. P. chaba stem methanolic (PCSM) extract produced time and dose dependent cytotoxicity in luminal breast cancer cells (MCF-7 and T47D) with a minimal toxicity in breast normal cells (MCF-10A) at 10-100 µg/mL concentration. PCSM extract exerts 16.79 and 31.21 µg/mL IC for T47D and MCF-7 cells, respectively, in 48 h treatment. PCSM significantly arrests the T47D cells at the G0/G1 phase by reducing the CCND1 and CDK4 expression at mRNA and protein levels. PCSM extract treatment significantly altered nuclear morphology, mitochondria membrane potential, and production of reactive oxygen species in T47D cells at IC concentration. Extract treatment significantly altered the Bax/Bcl-2 ratio and altered caspase 8 and 3 mRNA/protein levels in T47D cells. Confocal microscopy showed an increase in late apoptosis in PCSM extract-treated breast cancer cells at IC . Further, an increased caspase 9 and caspase 3/7 enzymatic activity was observed in test cells compared with nontreated cells. In conclusion, P. chaba phytocompound possesses the potential to induce cell cycle arrest and induce apoptosis in luminal breast cancer cells.
Topics: Humans; Female; Plant Extracts; Piper; Cell Line, Tumor; Breast Neoplasms; S Phase; Spices; Apoptosis; Cell Cycle; MCF-7 Cells; RNA, Messenger; Cell Proliferation
PubMed: 37711079
DOI: 10.1002/cbf.3857 -
Journal of Enzyme Inhibition and... Dec 2024The Libyan Strawberry, Pampan (ARB), is an endemic Jebel Akhdar plant used for traditional medicine. This study presents the antioxidant and hepatoprotective properties...
The Libyan Strawberry, Pampan (ARB), is an endemic Jebel Akhdar plant used for traditional medicine. This study presents the antioxidant and hepatoprotective properties of ARB fruit-extract. ARB phytochemical analysis indicated the presence of 354.54 GAE and 36.2 RE of the phenolics and flavonoids. LC-MS analysis identified 35 compounds belonging to phenolic acids, procyanidins, and flavonoid glycosides. Gallic acid, procyanidin dimer B3, β-type procyanidin trimer C, and quercetin-3-O-glucoside were the major constituents of the plant extract. ARB administration to paracetamol (PAR)-intoxicated rats reduced serum ALT, AST, bilirubin, hepatic tissue MDA and proinflammatory markers; TNF-α and IL-6 with an increase in tissue GSH level and SOD activity. Histological and immunohistochemical studies revealed that ARB restored the liver histology and significantly reduced the tissue expression of caspase 3, IL-1B, and NF-KB in PAR-induced liver damage. Docking analysis disclosed good binding affinities of some compounds with XO, COX-1, 5-LOX, and PI3K.
Topics: Rats; Animals; Antioxidants; Fruit; Angiotensin Receptor Antagonists; Angiotensin-Converting Enzyme Inhibitors; Plant Extracts; Liver; Flavonoids; Oxidative Stress
PubMed: 38153110
DOI: 10.1080/14756366.2023.2293639 -
Molecules (Basel, Switzerland) Oct 2023Tick and mite infestations pose significant challenges to animal health, agriculture, and public health worldwide. The search for effective and environmentally friendly...
Tick and mite infestations pose significant challenges to animal health, agriculture, and public health worldwide. The search for effective and environmentally friendly acaricidal agents has led researchers to explore natural alternatives. In this study, we investigated the acaricidal potential of the plant extract against ticks and mites. Additionally, we employed a computational approach to identify phytochemicals from the extract that could serve as drug candidates against these ectoparasites. The contact bioassay results demonstrated that the plant extract exhibited significant efficacy against and , with higher concentrations outperforming the positive control acaricide permethrin in terms of mite mortality. Time exposure to the extract also showed a positive correlation with better lethal concentration (LC and LC) values. Similarly, the adult immersion test revealed a notable inhibition of tick oviposition via the plant extract, especially at higher concentrations. The two-protein primary structure, secondary structure and stability were predicted using the Expasy's ProtParam server, SOPMA and SUSUI server, respectively. Using Homology modeling, the 3D structure of the protein was obtained and validated through the ERRAT server, and active sites were determined through the CASTp server. The docking analysis revealed that Alpha-Amyrenyl acetate and alpha-Tocopherol exhibited the highest docking scores for and aspartic protease proteins, respectively. These phytochemicals demonstrated strong binding interactions, suggesting their potential as acaricidal drug candidates. In conclusion, the plant extract displayed significant acaricidal activity against and Moreover, the computational approach identified promising phytochemicals that could serve as potential drug candidates for controlling these ectoparasites.
Topics: Animals; Female; Rhipicephalus; Sarcoptes scabiei; Larva; Plant Extracts; Plants; Phytochemicals; Acaricides
PubMed: 37836773
DOI: 10.3390/molecules28196930 -
BMC Complementary Medicine and Therapies Oct 2023Breast cancer is the most common malignancy in women, and medicinal plants can prevent and play an inhibitory role for cancer. This study aims to evaluate the anticancer...
BACKGROUND
Breast cancer is the most common malignancy in women, and medicinal plants can prevent and play an inhibitory role for cancer. This study aims to evaluate the anticancer effect of colchicum autumnale L. Corm on breast cancer cell models.
METHODS
In this study, the alkaloid-rich extract was prepared using the percolation method and with methanol/water solvent (70:30). HFF2 normal cell line and MCF-7 breast cancer cell line were cultured in microplates (96 wells). Then cells were treated with concentrations of 62.5 to 2000 ng/ml of extract and concentrations of 62 to 1000 ng/ml of doxorubicin at regular intervals of 48 and 72 h, and the percentage of cell growth inhibition was calculated. Cytotoxicity of drugs was measured by the MTT assay method. IC50 values were calculated by Calcusyn software. Also, the P-value of < 0.05 was considered significant.
RESULTS
Alkaloid-rich extract of Colchicum autumnale plant inhibited breast cancer cell growth (MCF-7). The IC50 parameter showed more cytotoxic effects of Colchicum autumnale plant extract on the MCF-7 cancer cell line than HFF2 normal cell line for 48 and 72 h. In addition, with higher concentrations of the extract, cytotoxicity, and growth inhibitory effect increased significantly and in comparison to the doxorubicin was almost the same as cytotoxic.
CONCLUSION
This research provides a novel view into the development of new drugs for the treatment of cancer diseases. Colchicum autumnale plant extract had a significant cytotoxic effect like Doxorubicin drug on breast cancer cell line (MCF-7), which can alternatively treat and prevent breast cancer.
Topics: Female; Humans; Colchicum; Breast Neoplasms; Antineoplastic Agents; Plant Extracts; Doxorubicin; Alkaloids
PubMed: 37805533
DOI: 10.1186/s12906-023-04189-0 -
PeerJ 2024Malaria has been appraised as a significant vector-borne parasitic disease with grave morbidity and high-rate mortality. Several challenges have been confronting the...
BACKGROUND
Malaria has been appraised as a significant vector-borne parasitic disease with grave morbidity and high-rate mortality. Several challenges have been confronting the efficient diagnosis and treatment of malaria.
METHOD
Google Scholar, PubMed, Web of Science, and the Egyptian Knowledge Bank (EKB) were all used to gather articles.
RESULTS
Diverse biochemical and physiological indices can mirror complicated malaria e.g., hypoglycemia, dyslipidemia, elevated renal and hepatic functions in addition to the lower antioxidant capacity that does not only destroy the parasite but also induces endothelial damage. Multiple trials have been conducted to improve recent points of care in malaria involving biosensors, lap on-chip, and microdevices technology. Regarding recent therapeutic trials, chemical falcipain inhibitors and plant extracts with anti-plasmodial activities are presented. Moreover, antimalaria nano-medicine and the emergence of nanocarrier (either active or passive) in drug transportation are promising. The combination therapeutic trials e.g., amodiaquine + artemether + lumefantrine are presented to safely counterbalance the emerging drug resistance in addition to the Tafenoquine as a new anti-relapse therapy.
CONCLUSION
Recognizing the pathophysiology indices potentiate diagnosis of malaria. The new points of care can smartly manipulate the biochemical and hematological alterations for a more sensitive and specific diagnosis of malaria. Nano-medicine appeared promising. Chemical and plant extracts remain points of research.
Topics: Humans; Antimalarials; Malaria, Falciparum; Artemisinins; Malaria; Plant Extracts
PubMed: 38529311
DOI: 10.7717/peerj.17084 -
International Journal of Molecular... Nov 2023Oxidative stress has been associated with different diseases, and different medicinal plants have been used to treat or prevent this condition. The leaf ethanolic...
Oxidative stress has been associated with different diseases, and different medicinal plants have been used to treat or prevent this condition. The leaf ethanolic extract (EE) and aqueous extract (AE) from have previously been characterized to have antioxidant potential in vitro and in vivo. In this study, we worked with EE and AE and two partition phases, AF (ethyl acetate) and BF (butanol), from AE extract. These extracts and partition phases did not display cytotoxicity. The EE and AE reduced NO production and ROS in all three concentrations tested. Furthermore, it was observed that EE and AE at 500 μg/mL concentration were able to reduce phagocytic activity by 30 and 50%, respectively. A scratch assay using a fibroblast cell line (NHI/3T3) showed that extracts and fractions induced cell migration with 60% wound recovery within 24 h, especially for BF. It was also observed that AF and BF had antioxidant potential in all the assays evaluated. In addition, copper chelation was observed. This activity was previously not detected in AE. The HPLC-DAD analysis showed the presence of phenolic compounds such as -cumaric acid and vitexin for extracts, while the GNPS annotated the presence of isoorientin, vitexin, kanakugiol, and tryptamine in the BF partition phase. The data presented here demonstrated that the EE, AE, AF, and BF of have potential immunomodulatory effects, antioxidant effects, as well as in vitro wound healing characteristics, which are important for dynamic inflammation process control.
Topics: Antioxidants; Wound Healing; Oxidative Stress; Phenols; Cell Line; Plant Extracts; Ethanol; Plant Leaves
PubMed: 37958868
DOI: 10.3390/ijms242115885 -
Journal of Medical Entomology Sep 2023In the search for effective and environmentally friendly mosquito control agents, we have examined natural sources, such as microbes and plants, and the synthetic...
In the search for effective and environmentally friendly mosquito control agents, we have examined natural sources, such as microbes and plants, and the synthetic analogs of natural products. These plants and microbes have evolved in their ecological niches to produce defensive compounds against other competing organisms in their surroundings such as microbes, plants, and insects as a means to enhance their survival. Thus, some of these plants and microbes have bioactive compounds with insecticidal, fungicidal, and phytotoxic activities. In our previous research, we successfully isolated bioactive constituents from natural sources. We have carried out synthetic modifications and total synthesis of marginally active isolated compounds to achieve significantly higher active compounds. We have focused on plants in the Rutaceae family as the members of this family are known to possess bioactive compounds with algicidal, antifungal, insecticidal, and fungicidal activities. In this article, we report the isolation and structure elucidation of mosquito larvicidal constituents from Poncirus trifoliata (Rutaceae) root extract.
Topics: Animals; Poncirus; Plant Extracts; Insecticides; Larva; Aedes
PubMed: 37409987
DOI: 10.1093/jme/tjad086