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Toxicon : Official Journal of the... Jun 2024This review analyzes the current progress in loaded nanoparticles (NPs) of plant extracts or isolated antineoplastic compounds used in breast and cervical cancer... (Review)
Review
This review analyzes the current progress in loaded nanoparticles (NPs) of plant extracts or isolated antineoplastic compounds used in breast and cervical cancer treatments. Also, it provides a comprehensive overview of the contributions made by traditional medicine and nanomedicine to the research of two of the most prevalent types of cancer in women worldwide: breast and cervical cancer. Searches were conducted in electronic databases to gather relevant information related to the biological activity of the NPs, which were meticulously reviewed. Nanomedicine has advanced to incorporate plant compounds including their crude extracts, in the preparation of NPs. The most used method is green synthesis, whose most outstanding advantages, is the reduced preparation time, and the variety of results that can be obtained depending on the reaction times, pH, temperature, and concentration of both the bio-reducing agent and the compound or plant extract. Most of the studies focus on evaluating crude extracts with high polarity, such as aqueous, alcoholic, and hydroalcoholic extracts. In conclusion, exploring the use of organic compounds is considered an area of opportunity for further research and future perspectives. Most of the analyzed studies were conducted using in vitro assays, highlighting the relatively recent nature of this field. It is expected that future research will involve more in vivo assays, particularly focusing on isolated cell lines representing the most difficult-to-treat types of cancer, such as triple-negative breast cancer like MDA-MB-231. Notably the MCF-7 cell line is one of the most used, while limited studies were found concerning cervical cancer.
Topics: Humans; Plant Extracts; Uterine Cervical Neoplasms; Breast Neoplasms; Female; Nanoparticles; Antineoplastic Agents, Phytogenic; Nanomedicine
PubMed: 38740098
DOI: 10.1016/j.toxicon.2024.107753 -
Journal of Ethnopharmacology Jan 2024Cyperus iria L. is a sedge belongs to Cyperaceae family. Tuber of this plant is traditionally used in fever.
ETHNOPHARMACOLOGICAL RELEVANCE
Cyperus iria L. is a sedge belongs to Cyperaceae family. Tuber of this plant is traditionally used in fever.
AIM OF THE STUDY
This study aimed to verify the effectiveness of this plant part against fever. Additionally, antinociceptive effect of the plant was evaluated.
MATERIALS AND METHODS
Antipyretic effect was evaluated by yeast induced hyperthermia experiment. Antinociceptive effect was determined by acetic acid induced writhing test and hot plate test. Four different doses of plant extract were used in mice model.
RESULTS
Extract at dose of 400 mg/kg.bw produced greater effect than paracetamol; reduction of elevated mice body temperature was observed by 2.6 °F and 4.2 °F after 4 h by paracetamol and 400 mg/kg.bw extract respectively. In acetic acid writhing test, extract at 400 mg/kg.bw and diclofenac were found to have equivalent effects producing percentage inhibition of writhing of 67.68% and 68.29% respectively. In hot plat test, significant reduction in latency was also observed after administration of plant extracts. Mean percent maximal effect was 83.55% and 67.26% for ketorolac and extract (400 mg/kg.bw) respectively.
CONCLUSION
Our study endorsed the traditional use of C. iria tuber in fever with possible antinociceptive effects.
Topics: Mice; Animals; Antipyretics; Analgesics; Acetaminophen; Methanol; Plant Extracts; Fever; Saccharomyces cerevisiae; Acetic Acid
PubMed: 37419227
DOI: 10.1016/j.jep.2023.116860 -
Scientific Reports Jul 2023This study focuses on the efficient and cost-effective synthesis of silver nanoparticles (AgNPs) using plant extracts, which have versatile and non-toxic applications....
This study focuses on the efficient and cost-effective synthesis of silver nanoparticles (AgNPs) using plant extracts, which have versatile and non-toxic applications. The research objectives include synthesizing AgNPs from readily available plant extracts, optimizing their production and multi scale characterization, along with exploring their use for enzyme immobilization and mitigation of poultry feather waste. Among the plant extracts tested, the flower extract of Hibiscus rosa-sinensis (HF) showed the most potential for AgNP synthesis. The synthesis of HF-mediated AgNPs was optimized using response surface methodology (RSM) for efficient and environment friendly production. Additionally, the keratinase enzyme obtained from Bacillus sp. NCIM 5802 was covalently linked to AgNPs, forming a keratinase nanocomplex (KNC) whose biochemical properties were evaluated. The KNC demonstrated optimal activity at pH 10.0 and 60 °C and it displayed remarkable stability in the presence of various inhibitors, metal ions, surfactants, and detergents. Spectroscopic techniques such as FTIR, UV-visible, and X-ray diffraction (XRD) analysis were employed to investigate the formation of biogenic HF-AgNPs and KNC, confirming the presence of capping and stabilizing agents. The morphological characteristics of the synthesized AgNPs and KNC were determined using transmission electron microscopy (TEM) and particle size analysis. The study highlighted the antimicrobial, dye scavenging, and antioxidant properties of biogenic AgNPs and KNC, demonstrating their potential for various applications. Overall, this research showcases the effectiveness of plant extract-driven green synthesis of AgNPs and the successful development of keratinase-laden nanocomplexes, opening possibilities for their use in immobilizing industrial and commercial enzymes.
Topics: Animals; Anti-Bacterial Agents; Silver; Keratins; Feathers; Metal Nanoparticles; X-Ray Diffraction; Plant Extracts; Spectroscopy, Fourier Transform Infrared
PubMed: 37463953
DOI: 10.1038/s41598-023-38721-6 -
Microbial Physiology 2024The current study investigated the antioxidant and anti-inflammatory effects of ethanol extracts from Lindera glauca twig (LGT) and leaf/stem (LGLS).
INTRODUCTION
The current study investigated the antioxidant and anti-inflammatory effects of ethanol extracts from Lindera glauca twig (LGT) and leaf/stem (LGLS).
METHODS
The antioxidant activities were measured by total content of polyphenol and flavonoid, DPPH radical scavenging, and ABTS+ radical scavenging activity. To evaluate the anti-inflammatory effect in the LPS-induced RAW 264.7 cells, protein and mRNA expression of major inflammatory factors were analyzed using Western blot analysis and RT-PCR.
RESULTS
The total polyphenol content of LGT and LGLS was 88.45 ± 11.74 and 115.75 ± 7.87 GA mg/g, respectively. The total flavonoid content was 66 ± 2.89 and 74.33 ± 2.89 QE mg/g. Both LGT and LGLS showed high DPPH and ABTS+ radical scavenging activities. Neither LGT nor LGLS was cytotoxic to RAW 264.7 cells. The anti-inflammatory activities were measured by LPS-induced RAW 264.7 cells. LGT and LGLS showed inhibition of the LPS-induced production of nitric oxide (NO), inducible NO synthase, cyclooxygenase-2 at the protein and mRNA levels, as determined by Western blotting and RT-PCR, respectively. In addition, the release of tumor necrosis factor-α and interleukin-6 mRNA expression levels of these cytokines was reduced by LGT and LGLS.
CONCLUSION
These results suggest that LGT and LGLS extracts have potential for use as a functional antioxidant and anti-inflammatory ingredient in cosmetic industry.
Topics: Animals; Mice; Anti-Inflammatory Agents; Plant Extracts; Lindera; Antioxidants; Plant Leaves; Nitric Oxide; Macrophages; Flavonoids; Cyclooxygenase 2; Lipopolysaccharides; RAW 264.7 Cells; Polyphenols; Cell Line; Plant Stems; Cell Survival; Nitric Oxide Synthase Type II
PubMed: 38286118
DOI: 10.1159/000536491 -
Molecules (Basel, Switzerland) Nov 2023In the present research, leaf extracts were utilized as reductants to bio-fabricate silver nanoparticles (LC-AgNPs) and this was followed by the evaluation of their...
In the present research, leaf extracts were utilized as reductants to bio-fabricate silver nanoparticles (LC-AgNPs) and this was followed by the evaluation of their antioxidant, antibacterial, and anticancer potential. Multiple parameters were optimized for the formation and fidelity of LC-AgNPs. The color shift of the reaction mixture from yellow to dark brown confirmed the LC-AgNPs formation. UV/VIS spectroscopy exhibited a surface plasmon resonance (SPR) band at 436 nm. The Fourier transform infrared (FTIR) spectroscopy spectrum depicted phytochemicals in the plant extract acting as bio-reducers for LC-AgNPs synthesis. The XRD pattern confirmed the presence of LC-AgNPs by showing peaks corresponding to 2θ angle at 8.24° (111), 38.16° (200), 44.20° (220), and 64.72° (311). Zetasizer analysis exhibited size distribution by intensity of LC-AgNPs with a mean value of 255.7 d. nm. Moreover, the zeta potential indicated that the AgNPs synthesized were stable. The irregular shape of LC-AgNPs with a mean average of 38.46 ± 0.26 nm was found by scanning electron microscopy. Furthermore, the antioxidant potential of LC-AgNPs was examined using a DPPH assay and was calculated to be higher in LC-AgNPs than in leaf extracts. The calculated IC values of the LC-AgNPs and plant extract are 85.01 ± 0.17 and 209.44 ± 0.24, respectively. The antibacterial activity of LC-AgNPs was investigated against , , and as well as and maximum potential was observed after 24 h against . Moreover, LC-AgNPs exhibited maximum anticancer potential against TPC1 cell lines compared to the plant extract. The findings suggested that LC-AgNPs could be used as antioxidant, antibacterial, and anticancer agents for the cure of free-radical-oriented bacterial and oncogenic diseases.
Topics: Silver; Antioxidants; Metal Nanoparticles; Anti-Bacterial Agents; Free Radicals; Spectroscopy, Fourier Transform Infrared; Plant Extracts
PubMed: 38067504
DOI: 10.3390/molecules28237773 -
Reproductive Biology Dec 2023Maternal diabetes can influence the development of offspring during fetal life and postnatally. Curatella americana is a plant used as a menstrual cycle regulator and to...
Maternal diabetes can influence the development of offspring during fetal life and postnatally. Curatella americana is a plant used as a menstrual cycle regulator and to prevent diabetes. This study evaluates the effects of C. americana aqueous extract on the estrous cycle and preimplantation embryos of adult female pups from diabetic rats. Female Sprague Dawley newborn rats received Streptozotocin or vehicle (citrate buffer). At adulthood, were submitted to the Oral Glucose Tolerance Test, and mated. The female rats were obtained and were distributed into four experimental groups: OC and OC/T represent female pups of control mothers and received water or plant extract, respectively; OD and OD/T represent female pups of diabetic mothers and received water or plant extract, respectively. The estrous cycle was followed for 10 days, the rats were mated and on gestational day 4 was performed preimplantation embryo analysis. Phenolic composition and biogenic amines in the extract were analyzed about the influence of the thermal process. The female pups from diabetic dams exhibited glucose intolerance, irregular estral cycle and a higher percentage of pre-embryos in delayed development (morula stage). After C. americana treatment, OD/T group no present a regular estrous cycle. Furthermore, the infusion process increases phenolic compounds and biogenic amines levels, which can have anti-estrogenic effect, anticipates the early embryonic development, and impair pre-implantation embryos. Thus, the indiscriminate use of medicinal plants should be avoided in any life phases by women, especially during pregnancy.
Topics: Humans; Pregnancy; Rats; Animals; Female; Adult; Rats, Sprague-Dawley; Dilleniaceae; Diabetes Mellitus, Experimental; Plant Extracts; Embryonic Development; Water; Biogenic Amines
PubMed: 37918046
DOI: 10.1016/j.repbio.2023.100819 -
PloS One 2024The emergence and spread of antimalarial drug resistance have become a significant problem worldwide. The search for natural products to develop novel antimalarial drugs...
Assessment of antimalarial activity of crude extract of Chan-Ta-Lee-La and Pra-Sa-Chan-Dang formulations and their plant ingredients for new drug candidates of malaria treatment: In vitro and in vivo experiments.
The emergence and spread of antimalarial drug resistance have become a significant problem worldwide. The search for natural products to develop novel antimalarial drugs is challenging. Therefore, this study aimed to assess the antimalarial and toxicological effects of Chan-Ta-Lee-La (CTLL) and Pra-Sa-Chan-Dang (PSCD) formulations and their plant ingredients. The crude extracts of CTLL and PSCD formulations and their plant ingredients were evaluated for in vitro antimalarial activity using Plasmodium lactate dehydrogenase enzyme and toxicity to Vero and HepG2 cells using the tetrazolium salt method. An extract from the CTLL and PSCD formulations exhibiting the highest selectivity index value was selected for further investigation using Peter's 4-day suppressive test, curative test, prophylactic test, and acute oral toxicity in mice. The phytochemical constituents were characterized using gas chromatography-mass spectrometry (GC-MS). Results showed that ethanolic extracts of CTLL and PSCD formulations possessed high antimalarial activity (half maximal inhibitory concentration = 4.88, and 4.19 g/mL, respectively) with low cytotoxicity. Ethanolic extracts of the CTLL and PSCD formulations demonstrated a significant dose-dependent decrease in parasitemia in mice. The ethanolic CTLL extract showed the greatest suppressive effect after 4 days of suppressive (89.80%) and curative (35.94%) testing at a dose of 600 mg/kg. Moreover, ethanolic PSCD extract showed the highest suppressive effect in the prophylactic test (65.82%) at a dose of 600 mg/kg. There was no acute toxicity in mice treated with ethanolic CTLL and PSCD extracts at 2,000 mg/kg bodyweight. GC-MS analysis revealed that the most abundant compounds in the ethanolic CTLL extract were linderol, isoborneol, eudesmol, linoleic acid, and oleic acid, whereas ethyl 4-methoxycinnamate was the most commonly found compound in the ethanolic PSCD extract, followed by 3-hydroxy-2-(4-hydroxy-3-methoxyphenyl)-4H-chromen-4-one, flamenol, oleic acid amide, linoleic acid, and oleic acid. In conclusions, ethanolic CTLL and PSCD extracts exhibited high antimalarial efficacy in vitro. The ethanolic CTLL extract at a dose of 600 mg/kg exhibited the highest antimalarial activity in the 4-day suppressive and curative tests, whereas the ethanolic PSCD extract at a dose of 600 mg/kg showed the highest antimalarial activity in the prophylactic test.
Topics: Animals; Mice; Antimalarials; Linoleic Acid; Oleic Acid; Plant Extracts; Malaria; Complex Mixtures; Plasmodium berghei
PubMed: 38206944
DOI: 10.1371/journal.pone.0296756 -
Biotechnology and Applied Biochemistry Dec 2023Silver nanoparticles (Ag NPs) via green synthesis using medicinal plants have been widely used in natural product research due to the economical and eco-friendly...
Preparation of silver nanoparticles by Osbeckia stellata aqueous extract via green synthesis approach: Characterization and assessment of their antioxidant, antidiabetic, cytotoxicity, and antibacterial properties.
Silver nanoparticles (Ag NPs) via green synthesis using medicinal plants have been widely used in natural product research due to the economical and eco-friendly properties of NPs. The plant-derived Ag NPs biosynthesis comprises the interaction between silver nitrate (precursor) and bioactive components of plant extract (reducing agents). In this work, Ag NPs were biosynthesized using Osbeckia stellata leaves aqueous extract. Characterization of Ag NPs was done by using ultraviolet-visible absorption (UV-Vis) spectroscopy, dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and energy-dispersive X-ray analysis (EDX). Further, antioxidant, antidiabetic, cytotoxicity, and antimicrobial activities were evaluated to establish the pharmacological properties of Ag NPs. UV-Vis spectroscopy and FTIR showed an absorption peak of Ag NPs due to the surface plasmonic resonance. In contrast, the particle size in the nanometer range was analyzed by XRD and DLS. The size of the particle was confirmed by the SEM, TEM, and EDX in the nanometer range. This study showed the spherical shape and crystalline nature of NPs. Zeta potential was used to determine the stability of Ag NPs. Biosynthesized Ag NPs showed significantly potent antioxidant, antidiabetic, and cytotoxicity activity. Ag NPs also showed effectiveness against gram-positive (Escherichia coli) and gram-negative (Staphylococcus aureus) bacteria in the antimicrobial activity study. The result concluded that these Ag NPs might be used in biomedical and pharmacological fields.
Topics: Antioxidants; Hypoglycemic Agents; Metal Nanoparticles; Microbial Sensitivity Tests; Silver; Anti-Bacterial Agents; Spectroscopy, Fourier Transform Infrared; Plant Extracts
PubMed: 37700428
DOI: 10.1002/bab.2512 -
Anti-inflammatory & Anti-allergy Agents... 2024, sometimes called Indian jujube or Ber, belongs to the Rhamnaceae group of plants. The aqueous and ethanolic Ziziphus mauritiana formulations were shown to have...
INTRODUCTION
, sometimes called Indian jujube or Ber, belongs to the Rhamnaceae group of plants. The aqueous and ethanolic Ziziphus mauritiana formulations were shown to have analgesic, antipyretic, potent analgesic, anti-inflammatory, and anti-emetic properties.
AIMS & OBJECTIVES
The aim of this study is to investigate the sedative and anticonvulsant activities of extract by governing 200 and 400 mg/kg body weight orally.
MATERIALS AND METHODS
The leaves are extracted with ethanol and lukewarm water with a soxhlet apparatus for 72 hours. After that acute extract toxicity study was performed and then locomotor activity, pentobarbital induced sleeping time and anticonvulsant activity were performed with the extract.
RESULTS
Oral administration of extract at dosages of 200 & 400 mg/kg was employed after an immediate toxicity test. At a dosage of 400 mg/kg, the number of locomotions was reduced significantly lengthened the period of time spent sleeping and there was showed a dosage-dependent reduction in all phases of an epileptic episode.
CONCLUSION
In this study, the extract reduced locomotor activity, however, it had a superior profile for an antiepileptic action than phenytoin since it decreased locomotor activity to a lesser level. The considerable increase in pentobarbitone sleep hours with the extracts at a higher dose supported the sedative action of .
Topics: Animals; Anticonvulsants; Ziziphus; Plant Extracts; Hypnotics and Sedatives; Mice; Male; Rats; Sleep; Plant Leaves; Female; Seizures; Rats, Wistar
PubMed: 38279726
DOI: 10.2174/0118715230276586231215045816 -
Advances in Colloid and Interface... Nov 2023This article provides an in-depth analysis of various fabrication methods of bimetallic nanoparticles (BNP), including chemical, biological, and physical techniques. The... (Review)
Review
A review of physical, chemical and biological synthesis methods of bimetallic nanoparticles and applications in sensing, water treatment, biomedicine, catalysis and hydrogen storage.
This article provides an in-depth analysis of various fabrication methods of bimetallic nanoparticles (BNP), including chemical, biological, and physical techniques. The review explores BNP's diverse uses, from well-known applications such as sensing water treatment and biomedical uses to less-studied areas like breath sensing for diabetes monitoring and hydrogen storage. It cites results from over 1000 researchers worldwide and >300 peer-reviewed articles. Additionally, the article discusses current trends, actionable recommendations, and the importance of synthetic analysis for industry players looking to optimize manufacturing techniques for specific applications. The article also evaluates the pros and cons of various fabrication methods, highlighting the potential of plant extract synthesis for mass production of capped BNPs. However, it warns that this method may not be suitable for certain applications requiring ligand-free surfaces. In contrast, physical methods like laser ablation offer better control and reactivity, especially for applications where ligand-free surfaces are critical. The report underscores the environmental benefits of plant extract synthesis compared to chemical methods that use hazardous chemicals and pose risks to extraction, production, and disposal. The article emphasizes the need for life cycle assessment (LCA) articles in the literature, given the growing volume of research on nanotechnology materials. This article caters to researchers at all stages and applies to various fields applying nanomaterials.
Topics: Metal Nanoparticles; Nanotechnology; Nanostructures; Catalysis; Plant Extracts
PubMed: 37804661
DOI: 10.1016/j.cis.2023.103010