-
Biology of Reproduction Jan 2024Maternal exposure to microplastics and nanoplastics has been shown to result in fetal growth restriction in mice. In this study, we investigated the placental and fetal...
Maternal exposure to microplastics and nanoplastics has been shown to result in fetal growth restriction in mice. In this study, we investigated the placental and fetal hemodynamic responses to plastics exposure in mice using high-frequency ultrasound. Healthy, pregnant CD-1 dams were given either 106 ng/L of 5 μm polystyrene microplastics or 106 ng/L of 50 nm polystyrene nanoplastics in drinking water throughout gestation and were compared with controls. Maternal exposure to both microplastics and nanoplastics resulted in evidence of placental dysfunction that was highly dependent on the particle size. The umbilical artery blood flow increased by 48% in the microplastic-exposed group and decreased by 25% in the nanoplastic-exposed group compared to controls (p < 0.05). The microplastic- and nanoplastic-exposed fetuses showed a significant decrease in the middle cerebral artery pulsatility index of 10% and 13%, respectively, compared to controls (p < 0.05), indicating vasodilation of the cerebral circulation, a fetal adaptation that is part of the brain sparing response to preserve oxygen delivery. Hemodynamic markers of placental dysfunction and fetal hypoxia were more pronounced in the group exposed to polystyrene nanoplastics, suggesting nanoplastic exposure during human pregnancy has the potential to disrupt fetal brain development, which in turn may cause suboptimal neurodevelopmental outcomes.
Topics: Pregnancy; Female; Humans; Animals; Mice; Microplastics; Plastics; Polystyrenes; Placenta; Fetal Development
PubMed: 37724921
DOI: 10.1093/biolre/ioad126 -
Journal of Hazardous Materials Oct 2023Microplastics (MPs) pollution poses an emerging threat to aquatic biota, which could hinder their physiological processes. Recently various evidence has demonstrated the...
Microplastics (MPs) pollution poses an emerging threat to aquatic biota, which could hinder their physiological processes. Recently various evidence has demonstrated the toxic impacts of MPs on cellular and organismal levels, but still, the underlying molecular mechanism behind their toxicity remains ambiguous. The hypothalamic-pituitary-gonadal (HPG) axis regulates the synthesis and release of sex steroid hormones, and SIRT1 plays a vital role in this process. The current study aimed to elucidate the harmful effects of MPs on female reproduction via SIRT1 modulation. Healthy female zebrafish were exposed to different concentrations (50 and 500 µg/L) of polystyrene microplastics (PS-MPs). The results revealed a significant change in the gonadosomatic index (GSI) after exposure to PS-MPs. In addition, the decreased fecundity rate displayed an evident dosage effect, indicating that exposure to PS-MPs causes deleterious effects on fertilization. Furthermore, significantly enhanced levels of reactive oxygen species (ROS) and apoptotic signals through the TUNEL assay were evaluated in different treated groups. Moreover, morphological alterations in the gonads of zebrafish exposed to MPs were also observed through H&E staining. The subsequent change in plasma steroid hormone levels (E2/T ratio) showed an imbalance in hormonal homeostasis. Meanwhile, to follow PS-MPs' effects on the HPG axis via SIRT1 modulation and gene expression related to steroidogenesis, SIRT1/p53 pathway was evaluated through qPCR. The altered transcription levels of genes indicated the plausible interference of PS-MPs on the HPG axis function. Our in-silico molecular docking study proves that PS-MPs efficiently bind and inhibit endocrine receptors and SIRT1. Thus, these findings add to our understanding of the probable molecular mechanisms of reproductive impairment caused by PS-MPs in zebrafish.
Topics: Female; Animals; Zebrafish; Microplastics; Plastics; Polystyrenes; Sirtuin 1; Molecular Docking Simulation; Reproductive Health; Fertility; Reproduction
PubMed: 37639793
DOI: 10.1016/j.jhazmat.2023.132359 -
The Science of the Total Environment Sep 2023Nanoplastics (NPs) induce nephrotoxicity in mammals, but an understanding of the potential mechanism or amelioration strategies is lacking. Herein, we established the...
Nanoplastics (NPs) induce nephrotoxicity in mammals, but an understanding of the potential mechanism or amelioration strategies is lacking. Herein, we established the polystyrene nanoplastics (PS-NPs, 100 nm)-induced nephrotoxicity murine model, and investigated the potential molecular mechanism of docosahexaenoic acid-enriched phosphatidylserine (DHA-PS) alleviating effects. Based on the biochemical indices, H&E staining and kidney metabolomics, we found that PS-NPs did cause murine nephrotoxicity, mainly due to inflammation, oxidative stress, and lipid disturbance. DHA-PS administration alleviated these effects, mainly by decreasing renal levels of IL-6, IL-1β, TNF-α and MDA, increasing the level of IL-10, increasing the activities of SOD, GSH-Px, CAT, and alleviating lipid disturbance, mainly by modulating kidney glycerophospholipid metabolism, linoleic acid metabolism and the SIRT1-AMPK pathway. This is the first time that the amelioration effects of DHA-PS on PS-NPs-induced nephrotoxicity have been investigated from multiple perspectives, providing a potential mechanism of nephrotoxicity caused by PS-NPs.
Topics: Animals; Mice; Phosphatidylserines; Microplastics; Polystyrenes; Docosahexaenoic Acids; Kidney; Nanoparticles; Water Pollutants, Chemical; Mammals
PubMed: 37308008
DOI: 10.1016/j.scitotenv.2023.164808 -
Chemosphere Sep 2023Sorption studies involving microplastics (MPs) are essential to understand the mechanisms implicated in contaminant retention. In this research, a complete study of the...
Sorption studies involving microplastics (MPs) are essential to understand the mechanisms implicated in contaminant retention. In this research, a complete study of the sorption behaviour of a hormonal contraceptive -levonorgestrel- in MPs of different composition in two distinct matrices was performed, using high-performance liquid chromatography coupled to a UV detector for the determination of levonorgestrel. Characterization of the studied MPs was achieved by X-ray diffraction, differential scanning calorimetry, and Fourier-transformed infrared spectroscopy. Kinetic and isotherm studies were performed using a batch design under controlled conditions: 500 mg of MPs pellets of 3-5 mm diameter, agitation at 125 rpm, and 30 °C. The comparison of results in ultrapure water and artificial seawater, revealed changes in sorption capacity, and the predominant sorption mechanisms involved. Overall, all studied MPs showed sorption affinity towards levonorgestrel, being low-density polyethylene the one with the highest sorption capacity in ultrapure water and polystyrene in seawater.
Topics: Microplastics; Polystyrenes; Polyethylene; Polypropylenes; Plastics; Levonorgestrel; Water; Water Pollutants, Chemical; Adsorption
PubMed: 37244556
DOI: 10.1016/j.chemosphere.2023.139042 -
Chemosphere Oct 2023Microplastics (MPs) can adsorb heavy metals and induce combined toxicity to aquatic organisms. However, the combined effects on the gut-liver and gut-brain axes are yet...
Microplastics (MPs) can adsorb heavy metals and induce combined toxicity to aquatic organisms. However, the combined effects on the gut-liver and gut-brain axes are yet to be fully comprehended. This study investigated the combined effects of polystyrene microplastics (PS-MPs) with two concentrations (20 and 200 μg/L) and three sizes (0.1, 10, and 250 μm) and Pb (50 μg/L) on zebrafish through gut-liver and gut-brain axes. The results showed that the combined exposure of 0.1 μm PS-MPs and Pb resulted in the most significant changes to the community diversity of gut microbiota. The combined exposures of PS-MPs (0.1 μm and 250 μm PS-MPs) and Pb significantly down-regulated expression of zo-1 and occludin but increased the lipopolysaccharide content in zebrafish liver compared to the PS-MPs or Pb alone exposure groups, indicating impaired gut barrier function. Subsequent studies showed that combined exposure of PS-MPs (0.1 μm and 250 μm) and Pb induced liver inflammation through the TLR4/NF-κB pathway. Moreover, all exposure groups had an impact on the expression of genes related to bile acid metabolism (cyp7a1, fgf19, abcb11b, and slc10a2) and neurotransmitters (tph1a, tph2, pink, and trh). The findings of this study provide new evidence on the combined effects of MPs and heavy metals, which are significant for their hazard identification and risk assessment.
Topics: Animals; Microplastics; Plastics; Zebrafish; Lead; Polystyrenes; Metals, Heavy; Water Pollutants, Chemical
PubMed: 37394195
DOI: 10.1016/j.chemosphere.2023.139383 -
The Science of the Total Environment Dec 2023The toxic effects of organic pollutants and nanoplastics on fish have been extensively studied, but there is limited research available on their combined toxicity to...
The toxic effects of organic pollutants and nanoplastics on fish have been extensively studied, but there is limited research available on their combined toxicity to bivalves. This research aimed to investigate the accumulation and ecotoxicological impacts such as antioxidant capacity, histopathology and intestinal microbiota in white hard clam Meretrix lyrata, resulting from 7 days of single and mixture exposure to 3,3',4,4'-tetrachlorobiphenyl (PCB77, 0.1 mg/L) and polystyrene nanoplastics (PS-NPs, 80 nm, 1 mg/L). Our findings revealed that PS-NPs accumulated in various tissues such as the intestine, gill, mantle, foot, and siphon. And when compared to the PCB-PSNPs (PP) co-exposure group, the intestinal fluorescence intensity mediated by plastic particles in the PS-NPs (PS group) was significantly higher. The gill, digestive gland, and intestine were all damaged to varying extent by single exposure to PS-NPs or PCB77, according to histopathological analysis, which was aggravated by PP group. Moreover, the co-exposure induced a higher level of oxidative stress, which reflected by increase of activities of superoxide dismutase, catalase, glutamate oxaloacetate transaminase and glutamic-pyruvic transaminase and malondialdehyde content. In addition, the intestine microbial composition was dramatically altered by the combined exposure, reducing the abundance of probiotics such as Firmicutes, thereby posing a great threat to the health and metabolism of M. lyrata. In conclusion, our findings showed that PS-NPs and PCB77 co-exposure induced a higher toxicity to M. lyrata, including histopathological changes, altered antioxidant capacity and intestinal microbiota disruption. This study provides novel insights into PCB77 and PS-NPs' combined toxicity to marine organisms and its underlying molecular mechanisms of ecotoxicological effects.
Topics: Animals; Antioxidants; Polystyrenes; Microplastics; Gastrointestinal Microbiome; Oxidative Stress; Bivalvia; Water Pollutants, Chemical; Nanoparticles
PubMed: 37722427
DOI: 10.1016/j.scitotenv.2023.167125 -
Environmental Health Perspectives Feb 2024Micro- and nanoplastics (MNPs) and homosalate (HMS) are ubiquitous emerging environmental contaminants detected in human samples. Despite the well-established...
BACKGROUND
Micro- and nanoplastics (MNPs) and homosalate (HMS) are ubiquitous emerging environmental contaminants detected in human samples. Despite the well-established endocrine-disrupting effects (EDEs) of HMS, the interaction between MNPs and HMS and its impact on HMS-induced EDEs remain unclear.
OBJECTIVES
This study aimed to investigate the influence of MNPs on HMS-induced estrogenic effects and elucidate the underlying mechanisms and .
METHODS
We assessed the impact of polystyrene nanospheres (PNSs; , ) on HMS-induced MCF-7 cell proliferation (HMS: , equivalent to ) using the E-SCREEN assay and explored potential mechanisms through transcriptomics. Adult zebrafish were exposed to HMS () with or without PNSs (, ) for 21 d. EDEs were evaluated through gonadal histopathology, fertility tests, steroid hormone synthesis, and gene expression changes in the hypothalamus-pituitary-gonad-liver (HPGL) axis.
RESULTS
Coexposure of HMS and PNSs resulted in higher expression of estrogen receptor () and the mRNAs of target genes (, , and ), a greater estrogen-responsive element transactivation activity, and synergistic stimulation on MCF-7 cell proliferation. Knockdown of serum and glucocorticoid-regulated kinase 1 (SGK1) rescued the MCF-7 cell proliferation induced by PNSs alone or in combination with HMS. In zebrafish, coexposure showed higher expression of and promoted ovary development but inhibited spermatogenesis. In addition, coexposure led to lower egg hatchability, higher embryonic mortality, and greater larval malformation. Coexposure also modulated steroid hormone synthesis genes (, , , , and ), and resulted in higher () release in females. Conversely, males showed lower testosterone, , and gene expressions of , , , , and .
DISCUSSION
PNS exposure exacerbated HMS-induced estrogenic effects via SGK1 up-regulation in MCF-7 cells and disrupting the HPGL axis in zebrafish, with gender-specific patterns. This offers new mechanistic insights and health implications of MNP and contaminant coexposure. https://doi.org/10.1289/EHP13696.
Topics: Adult; Female; Humans; Male; Animals; Nanospheres; Zebrafish; MCF-7 Cells; Polystyrenes; Estrogens; Glucocorticoids; Steroids
PubMed: 38381479
DOI: 10.1289/EHP13696 -
Marine Pollution Bulletin Sep 2023The current experiment measured the multifaceted effects of polystyrene and fluoranthene, acting alone or in a mixture, on the meiobenthic nematode species Oncholaimus...
Do functional traits and biochemical biomarkers of the nematode Oncholaimus campylocercoides De Coninck and Schuurmans Stekhoven, 1933 affected by fluoranthene and polystyrene microplastics? Results from a microcosm bioassay and molecular modeling.
The current experiment measured the multifaceted effects of polystyrene and fluoranthene, acting alone or in a mixture, on the meiobenthic nematode species Oncholaimus campylocercoides. This Oncholaimid was first experimentally selected from an entire nematode assemblage taken from the Jeddah coasts (Saudi Arabia). Several discernible changes were found in morphometry and functional traits after exposure to single and combined treatments. An increase in the activity of the biochemical biomarkers catalase and glutathione S-transferase was also observed following the exposure of males and gravid females of O. campylocercoides to 37.5 ng fluoranthene·g dry weight (DW) and 62.5 mg polystyrene·kg DW paralleled by a higher vulnerability of females. Moreover, the reproduction and feeding of this species were impaired, starting from 37.5 ng fluoranthene·g and 62.5 mg polystyrene·kg, respectively. These results have been confirmed by good binding affinities and molecular interactions of fluoranthene and polystyrene with both GLD-3 and SDP receptors.
Topics: Animals; Female; Male; Microplastics; Polystyrenes; Plastics; Nematoda; Biomarkers
PubMed: 37506479
DOI: 10.1016/j.marpolbul.2023.115294 -
Marine Pollution Bulletin Sep 2023This study investigates the abundances and composition of microplastics (MP) among the shallow layers of a coastal Mediterranean Marine Protected Area (Cabrera MPA),...
This study investigates the abundances and composition of microplastics (MP) among the shallow layers of a coastal Mediterranean Marine Protected Area (Cabrera MPA), seafloor sediments, hyperbenthic environment, and the water column. The mid waters samples were collected mid-way between the sea surface and the seafloor and hyperbenthic samples at the water layer adjacent to the seafloor. Sampling was carried out on patchiness seafloor of Posidonia oceanica meadows. The seafloor sediments showed a mean abundance of 378,769.20 ± 508,109.11 MPs/m, three orders of magnitude higher than the hyperbenthic (209.17 ± 117.07 MPs/m), and the mid waters layer (106.48 ± 107.17 MPs/m). An increasing vertical gradient in MP abundances, mainly composed of fibers was observed. Fibers were made-up mainly of polystyrene (PS, 25 %), expanded polystyrene (EPS, 18 %) and cellulose acetate (CA, 16 %). The results stress the need to increase efforts to find solutions to mitigate fiber pollution in the marine environment.
Topics: Plastics; Polystyrenes; Geologic Sediments; Water Pollutants, Chemical; Environmental Monitoring; Microplastics; Water; Ecosystem
PubMed: 37459771
DOI: 10.1016/j.marpolbul.2023.115291 -
Ecotoxicology and Environmental Safety Sep 2023As a new type of environmental pollutant, microplastics have been garnered increasing attention, especially in regard to their effects on the reproductive system....
As a new type of environmental pollutant, microplastics have been garnered increasing attention, especially in regard to their effects on the reproductive system. However, researchers have yet to report whether prevention and treatment measures exist for reproductive injury caused by microplastics. The aim of this study was therefore to explore the mechanism of spermatogenic injury induced by polystyrene microplastics (PS-MPs) and the intervention effect of probiotics based on the gut microbiota-testis axis. Mice were orally exposed for 35 days to 5 µm of PS-MPs with a gavage dose was 0.1 mg/day, and the intervention group was given probiotics (Lactobacillus, Bifidobacterium longum, and Enterococcus) orally. Fecal samples were then subjected to 16 S rRNA sequencing analysis, and sperm motion was analyzed by a Hamilton-Thorne Sperm analyzer. The results showed that PS-MPs exposed mice had significant spermatogenic dysfunction and testicular inflammation. In addition, the intestinal microbial structure of exposed mice changed significantly; the abundance of Lactobacillus decreased, and the abundance of Prevotella increased. Furthermore, with fecal microbiota transplantation, the recipient mice showed a significant decrease in sperm quality. However, probiotics supplementation helped inhibit the activation of IL-17A signaling driven by gut microbes, thereby alleviating the inflammatory response and improving sperm quality decline caused by PS-MPs. These results may provide a scientific basis for further understanding of the mechanism of male reproductive damage caused by environmental pollutants such as microplastics and for novel reproductive damage intervention measures.
Topics: Male; Animals; Mice; Microplastics; Plastics; Polystyrenes; Semen; Environmental Pollutants; Lactobacillus; Probiotics
PubMed: 37441951
DOI: 10.1016/j.ecoenv.2023.115248