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International Journal of Molecular... Sep 2023In this narrative review, we aim to point out the close relationship between mpox virus (MPXV) infection and the role of saliva as a diagnostic tool for mpox,... (Review)
Review
In this narrative review, we aim to point out the close relationship between mpox virus (MPXV) infection and the role of saliva as a diagnostic tool for mpox, considering the current molecular approach and in the perspective of OMICs application. The MPXV uses the host cell's rough endoplasmic reticulum, ribosomes, and cytoplasmic proteins to replicate its genome and synthesize virions for cellular exit. The presence of oral mucosa lesions associated with mpox infection is one of the first signs of infection; however, current diagnostic tools find it difficult to detect the virus before the rashes begin. MPXV transmission occurs through direct contact with an infected lesion and infected body fluids, including saliva, presenting a potential use of this fluid for diagnostic purposes. Currently available diagnostic tests for MPXV detection are performed either by real-time quantitative PCR (RT-qPCR) or ELISA, which presents several limitations since they are invasive tests. Despite current clinical trials with restricted sample size, MPXV DNA was detected in saliva with a sensitivity of 85%-100%. In this context, the application of transcriptomics, metabolomics, lipidomics, or proteomics analyses coupled with saliva can identify novel disease biomarkers. Thus, it is important to note that the identification and quantification of salivary DNA, RNA, lipid, protein, and metabolite can provide novel non-invasive biomarkers through the use of OMICs platforms aiding in the early detection and diagnosis of MPXV infection. Untargeted mass spectrometry (MS)-based proteomics reveals that some proteins also expressed in saliva were detected with greater expression differences in blood plasma when comparing mpox patients and healthy subjects, suggesting a promising alternative to be applied in screening or diagnostic platforms for mpox salivary diagnostics coupled to OMICs.
Topics: Humans; Pathology, Oral; Mpox (monkeypox); Communicable Diseases; Body Fluids; Saliva
PubMed: 37762664
DOI: 10.3390/ijms241814362 -
Skinmed 2023TikTok is the newest and the most popular form of social media for young global audiences. Healthcare professionals can present medical information to all audiences in...
TikTok is the newest and the most popular form of social media for young global audiences. Healthcare professionals can present medical information to all audiences in engaging and short-length videos. Our study evaluated the reliability of monkeypox-related information presented on TikTok. We assessed the quality of the information presented using DISCERN, a peer-reviewed tool used for evaluating health information presented to consumers. Findings from our study suggested that physicians, members of the healthcare field, and nonmedical individuals post videos for viewers with unreliable information. Physicians and other healthcare professionals should prioritize providing reliable and well-cited information about current infectious diseases, such as monkeypox, to the young and impressionable audiences using TikTok.
Topics: Humans; Mpox (monkeypox); Reproducibility of Results; Social Media; Physicians; Communication
PubMed: 37771014
DOI: No ID Found -
Advances in Experimental Medicine and... 2024The smallpox infection with the variola virus was one of the most fatal disorders until a global eradication was initiated in the twentieth century. The last cases were... (Review)
Review
The smallpox infection with the variola virus was one of the most fatal disorders until a global eradication was initiated in the twentieth century. The last cases were reported in Somalia 1977 and as a laboratory infection in the UK 1978; in 1980, the World Health Organization (WHO) declared smallpox for extinct. The smallpox virus with its very high transmissibility and mortality is still a major biothreat, because the vaccination against smallpox was stopped globally in the 1980s. For this reason, new antivirals (cidofovir, brincidofovir, and tecovirimat) and new vaccines (ACAM2000, LC16m8 and Modified Vaccine Ankara MVA) were developed. For passive immunization, vaccinia immune globulin intravenous (VIGIV) is available. Due to the relationships between orthopox viruses such as vaccinia, variola, mpox (monkeypox), cowpox, and horsepox, the vaccines (LC16m8 and MVA) and antivirals (brincidofovir and tecovirimat) could also be used in the mpox outbreak with positive preliminary data. As mutations can result in drug resistance against cidofovir or tecovirimat, there is need for further research. Further antivirals (NIOCH-14 and ST-357) and vaccines (VACΔ6 and TNX-801) are being developed in Russia and the USA. In conclusion, further research for treatment and prevention of orthopox infections is needed and is already in progress. After a brief introduction, this chapter presents the smallpox and mpox disease and thereafter full overviews on antiviral treatment and vaccination including the passive immunization with vaccinia immunoglobulins.
Topics: Smallpox; Humans; Antiviral Agents; Smallpox Vaccine; Mpox (monkeypox); Vaccination; Variola virus; Animals; Cytosine; Monkeypox virus; Immunization, Passive; Organophosphonates; Isoindoles; Cidofovir; Immunoglobulins, Intravenous; Benzamides; Phthalimides
PubMed: 38801586
DOI: 10.1007/978-3-031-57165-7_19 -
The Journal of Gene Medicine Sep 2023Monkeypox infection outbreaks have been observed sporadically in Africa, usually as a result of interaction with wildlife reservoirs. The genomes of the new strain range... (Review)
Review
Monkeypox infection outbreaks have been observed sporadically in Africa, usually as a result of interaction with wildlife reservoirs. The genomes of the new strain range in size from 184.7 to 198.0 kb and are identified with 143-214 open reading frames. Viral cores are rapidly carried on microtubules away from the cell's perimeter and deeper into the cytoplasm once the virus and cell membranes fuse. Depending on the kind of exposure, patients with monkeypox may experience a febrile prodrome 5-13 days after exposure, which frequently includes lymphadenopathy, malaise, headaches, and muscle aches. A different diagnostic approach is available for monkeypox, including histopathological analysis, electron microscopy, immunoassays, polymerase chain reaction, genome sequencing, microarrays, loop-mediated isothermal amplification technology and CRISPR (i.e., "clustered regularly interspaced short palindromic repeats"). There are currently no particular, clinically effective treatments available for the monkeypox virus. An initial treatment is cidofovir. As a monophosphate nucleotide analog, cidofovir is transformed into an inhibitor of viral DNA polymerase by cellular kinases, which is analogous to cidofovir's function in inhibiting viral DNA synthesis. The European Medicine Agency and the Food and Drug Administration have both granted permission for IMVAMUNE, a replication-deficient, attenuated third-generation modified vaccinia Ankara vaccine, to be used for the prevention of smallpox and monkeypox in adults.
Topics: United States; Humans; Mpox (monkeypox); Cidofovir; Vaccinia virus; Monkeypox virus; Clinical Laboratory Techniques
PubMed: 37132057
DOI: 10.1002/jgm.3521 -
Emerging Infectious Diseases Oct 2023The 2022-2023 mpox outbreak predominantly affected adult men; 1.3% of reported cases were in children and adolescents <18 years of age. Analysis of global surveillance...
The 2022-2023 mpox outbreak predominantly affected adult men; 1.3% of reported cases were in children and adolescents <18 years of age. Analysis of global surveillance data showed 1 hospital intensive care unit admission and 0 deaths in that age group. Transmission routes and clinical manifestations varied across age subgroups.
Topics: Adolescent; Child; Humans; Disease Outbreaks; Hospitalization; Intensive Care Units; Mpox (monkeypox)
PubMed: 37647121
DOI: 10.3201/eid2910.230516 -
Advances in Experimental Medicine and... 2024Variola virus is an anthroponotic agent that belongs to the orthopoxvirus family. It is an etiological agent of smallpox, an ancient disease that caused massive... (Review)
Review
Variola virus is an anthroponotic agent that belongs to the orthopoxvirus family. It is an etiological agent of smallpox, an ancient disease that caused massive mortality of human populations. Twentieth century has witnessed the death of about 300 million people due to the unavailability of an effective vaccine. Early detection is the primary strategy to prevent an outbreak of smallpox. Variola virus forms the characteristic pus-filled pustules and centrifugal rash distribution in the infected patients while transmission occurs mainly through respiratory droplets during the early stage of infection. No antiviral drugs are approved for variola virus till date. Generation of first-generation vaccines helped in the eradication of smallpox which was declared by the World Health Organization.
Topics: Humans; Variola virus; Smallpox; Animals; Smallpox Vaccine; Disease Outbreaks
PubMed: 38801576
DOI: 10.1007/978-3-031-57165-7_9 -
Advances in Experimental Medicine and... 2024The current multicounty outbreak of monkeypox virus (MPXV) posed an emerging and continued challenge to already strained public healthcare sector, around the globe.... (Review)
Review
The current multicounty outbreak of monkeypox virus (MPXV) posed an emerging and continued challenge to already strained public healthcare sector, around the globe. Since its first identification, monkeypox disease (mpox) remained enzootic in Central and West African countries where reports of human cases are sporadically described. Recent trends in mpox spread outside the Africa have highlighted increased incidence of spillover of the MPXV from animal to humans. While nature of established animal reservoirs remained undefined, several small mammals including rodents, carnivores, lagomorphs, insectivores, non-human primates, domestic/farm animals, and several species of wildlife are proposed to be carrier of the MPXV infection. There are established records of animal-to-human (zoonotic) spread of MPXV through close interaction of humans with animals by eating bushmeat, contracting bodily fluids or trading possibly infected animals. In contrast, there are reports and increasing possibilities of human-to-animal (zooanthroponotic) spread of the MPXV through petting and close interaction with pet owners and animal care workers. We describe here the rationales and molecular factors which predispose the spread of MPXV not only amongst humans but also from animals to humans. A range of continuing opportunities for the spread and evolution of MPXV are discussed to consider risks beyond the currently identified groups. With the possibility of MPXV establishing itself in animal reservoirs, continued and broad surveillance, investigation into unconventional transmissions, and exploration of spillover events are warranted.
Topics: Animals; Mpox (monkeypox); Humans; Monkeypox virus; Zoonoses; Disease Reservoirs; Disease Outbreaks; Animals, Wild
PubMed: 38801572
DOI: 10.1007/978-3-031-57165-7_5 -
Shanghai Kou Qiang Yi Xue = Shanghai... Aug 2023Monkeypox is becoming a viral infectious disease of global concern. WHO has reported monkeypox outbreaks in more than 50 countries. Since the first imported case has... (Review)
Review
Monkeypox is becoming a viral infectious disease of global concern. WHO has reported monkeypox outbreaks in more than 50 countries. Since the first imported case has been confirmed and reported by Taiwan, China, in June 2022, the monkeypox has draw high attention from the national public health and epidemic prevention department. Among the key tasks of Shanghai high-quality healthcare development in 2023, monkeypox has been identified as one of the key infectious diseases that need to be under strict prevention and control. The diagnosis and treatment in dental department are mainly performed face-to-face, with patients' masks taken-off. Large amount of aerosol spray will be generated during the operation. At the same time, dental diagnosis and treatment is in outpatient department, where the patient flow is large. Once monkeypox patients have been diagnosed and treated in the dental diagnosis and treatment area, and the preventive measures are not implemented, it will provide convenience for monkeypox to transmit. In order to avoid this kind of situation, this article made a review of monkey-pox from the following 3 aspects: epidemic transmission history of monkeypox, systemic and oral symptoms of monkeypox, and oral prevention of monkeypox, to improve the knowledge and prevention ability of dental medical staff on monkeypox for early recognition and prevention.
Topics: Humans; Mpox (monkeypox); China; Public Health; Outpatients; Quality of Health Care
PubMed: 38044724
DOI: No ID Found -
Clinical Microbiology and Infection :... Jul 2024Smallpox was a major cause of human mortality until its eradication, but the threat of orthopox viruses has not disappeared. Since the eradication of smallpox and the... (Review)
Review
BACKGROUND
Smallpox was a major cause of human mortality until its eradication, but the threat of orthopox viruses has not disappeared. Since the eradication of smallpox and the cessation of the related vaccination campaigns, the threat has been growing, as evidenced by the currently ongoing worldwide Mpox outbreak. In addition to threats of an evolving Mpox, we must also be aware of a myriad of other threats that remain. Many countries still lack biosecurity regulations reflecting the recent technological advances, and the threat of bioterrorism remains ever present. Reconstruction of smallpox is a distinct possibility, as are other scenarios whereby other orthopox viruses may be made more fit for transmission in humans.
OBJECTIVES
To outline and discuss potential biosafety and biosecurity threats posed by orthopox viruses.
SOURCES
Published scientific literature, news articles, and international agreements.
CONTENT AND IMPLICATIONS
It would be wise to take steps to mitigate these threats now. Vaccination campaigns should be considered in areas with frequent orthopox outbreaks, and more efforts must be made to put a final end to the Mpox outbreak. In many countries, national biosafety and biosecurity regulations may need to be revised and strengthened to better reflect the threats posed by new technologies, including controls on synthesis of smallpox sequences. Furthermore, more international cooperation and aid is needed. The present global Mpox outbreak could likely have been prevented had areas where Mpox is endemic not been neglected. Future outbreaks could be much worse.
Topics: Humans; Orthopoxvirus; Disease Outbreaks; Poxviridae Infections; Smallpox; Animals; Containment of Biohazards; Bioterrorism; Vaccination
PubMed: 38387500
DOI: 10.1016/j.cmi.2024.02.011 -
Infectious Disorders Drug Targets 2024Monkeypox is a viral disease; its outbreak was recently declared a global emergency by the World Health Organization. For the first time, a monkeypox virus... (Review)
Review
Monkeypox is a viral disease; its outbreak was recently declared a global emergency by the World Health Organization. For the first time, a monkeypox virus (MPXV)-infected patient was found in India. Various researchers back-to-back tried to find the solution to this health emergency just after COVID-19. In this review, we discuss the current outbreak status of India, its transmission, virulence factors, symptoms, treatment, and the preventive guidelines generated by the Indian Health Ministry. We found that monkeypox virus (MPXV) disease is different from smallpox, and the age group between 30-40 years old is more prone to MPXV disease. We also found that, besides homosexuals, gays, bisexuals, and non-vegetarians, it also affects normal straight men and women who have no history of travel. Close contact should be avoided from rats, monkeys and sick people who are affected by monkeypox. To date, there are no monkeypox drugs, but Tecovirimat is more effective than other drugs that are used for other viral diseases like smallpox. Therefore, we need to develop an effective antiviral agent against the virulence factor of MXPV.
Topics: Animals; Female; Humans; Male; Antiviral Agents; Benzamides; Disease Outbreaks; India; Isoindoles; Monkeypox virus; Mpox (monkeypox); Phthalimides; Virulence Factors; Adult
PubMed: 38243966
DOI: 10.2174/0118715265258451231214063506