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Differentiation; Research in Biological... Jun 2024Osteoclast (OC) differentiation, vital for bone resorption, depends on osteoclast and precursor fusion. Osteoprotegerin (OPG) inhibits osteoclast differentiation. OPG's...
Osteoclast (OC) differentiation, vital for bone resorption, depends on osteoclast and precursor fusion. Osteoprotegerin (OPG) inhibits osteoclast differentiation. OPG's influence on fusion and mechanisms is unclear. Osteoclasts and precursors were treated with OPG alone or with ATP. OPG significantly reduced OC number, area and motility and ATP mitigated OPG's inhibition. However, OPG hardly affected the motility of precusors. OPG downregulated fusion-related molecules (CD44, CD47, DC-STAMP, ATP6V0D2) in osteoclasts, reducing only CD47 in precursors. OPG reduced Connexin43 phosphorylated forms (P1 and P2) in osteoclasts, affecting only P2 in precursors. OPG disrupted subcellular localization of CD44, CD47, DC-STAMP, ATP6V0D2, and Connexin43 in both cell types. Findings underscore OPG's multifaceted impact, inhibiting multinucleated osteoclast and mononuclear precursor fusion through distinct molecular mechanisms. Notably, ATP mitigates OPG's inhibitory effect, suggesting a potential regulatory role for the ATP signaling pathway. This study enhances understanding of intricate processes in osteoclast differentiation and fusion, offering insights into potential therapeutic targets for abnormal bone metabolism.
PubMed: 38896972
DOI: 10.1016/j.diff.2024.100789 -
Academic Medicine : Journal of the... Jan 2024This scoping review aims to map the breadth of the literature examining how trust is defined in health care teams, describe what measurements of trust are used, and... (Review)
Review
PURPOSE
This scoping review aims to map the breadth of the literature examining how trust is defined in health care teams, describe what measurements of trust are used, and investigate the precursors and outcomes of trust.
METHOD
Five electronic databases (Ovid MEDLINE, CINAHL, PsycInfo, Embase, and ASSIA [Applied Social Sciences Index and Abstracts]) were searched alongside sources of gray literature in February 2021. To be included, studies needed to discuss a health care team directly involved in managing patient care and one aspect of trust as a relational concept. A content count of the definitions of trust and tools used to measure trust and a deductive thematic analysis of the precursors and outcomes of trust in health care teams were conducted.
RESULTS
Ultimately, 157 studies were included after full-text review. Trust was the main focus of 18 (11%) studies and was not routinely defined (38, 24%). Ability appeared to be key to the definition. Trust was measured in 34 (22%) studies, often using a bespoke measure (8/34, 24%). The precursors of trust within health care teams occur at the individual, team, and organizational levels. The outcomes of trust occur at the individual, team, and patient levels. Communication was a broad overarching theme that was present at all levels, both as a precursor and outcome of trust. Respect, as a precursor, influenced trust at the individual, team, and organizational levels, while trust influenced learning, an outcome, across the patient, individual, and team levels.
CONCLUSIONS
Trust is a complex, multilevel construct. This scoping review has highlighted gaps in the literature, including exploration of the swift trust model, which may be applicable to health care teams. Furthermore, knowledge from this review may be integrated into future training and health care practices to optimize team processes and teamworking.
Topics: Humans; Trust; Clinical Competence; Learning; Delivery of Health Care; Patient Care Team
PubMed: 37433205
DOI: 10.1097/ACM.0000000000005320 -
Gastroenterology Dec 2023Estimates on the progression of precursor lesions to pancreatic cancer (PC) are scarce. We used microsimulation modeling to gain insight into the natural disease course...
BACKGROUND & AIMS
Estimates on the progression of precursor lesions to pancreatic cancer (PC) are scarce. We used microsimulation modeling to gain insight into the natural disease course of PC and its precursors. This information is pivotal to explore the efficacy of PC screening.
METHODS
A Microsimulation Screening Analysis model was developed in which pancreatic intraepithelial neoplasms and cysts can evolve from low-grade dysplasia (LGD) to high-grade dysplasia (HGD) to PC. The model was calibrated to Dutch PC incidence data and Japanese precursor prevalence data (autopsy cases without PC) and provides estimates of PC progression (precursor lesion onset and stage duration).
RESULTS
Mean LGD state durations of cysts and pancreatic intraepithelial neoplasms were 15.8 years and 17.1 years, respectively. Mean HGD state duration was 5.8 years. For lesions that progress to PC, the mean duration was 4.8-4.9 years for LGD lesions and 4.0-4.1 years for HGD lesions. In 13.7% of individuals who developed PC, the HGD state lasted less than 1 year. The probability that an individual at age 50 years developed PC in the next 20 years was estimated to be 1.8% in the presence of any cyst and 6.1% in case of an LGD mucinous cyst. This 20-year PC risk was estimated to be 5.1% for individuals with an LGD pancreatic intraepithelial neoplasm.
CONCLUSIONS
Mean duration of HGD lesions before development of PC was estimated to be 4.0 years. This implies a window of opportunity for screening, presuming the availability of a reliable diagnostic test. The probability that an LGD cyst will progress to cancer was predicted to be low.
Topics: Humans; Middle Aged; Pancreatic Cyst; Pancreatic Neoplasms; Hyperplasia; Carcinoma in Situ; Adenocarcinoma
PubMed: 37633497
DOI: 10.1053/j.gastro.2023.08.027 -
ChemSusChem Apr 2024The conversion of biomass and natural wastes into carbon-based materials for various applications such as catalysts and energy-related materials is a fascinating and...
The conversion of biomass and natural wastes into carbon-based materials for various applications such as catalysts and energy-related materials is a fascinating and sustainable approach emerged during recent years. Precursor nature and characteristics are complex, hence, their effect on the properties of resulting materials is still unclear. In this work, we have investigated the effect of different precursors and pyrolysis temperature on the properties of produced carbon materials and their potential application as negative electrode materials in Li-ion batteries. Three biomasses, lignocellulosic brewery spent grain from a local brewery, catechol-rich lignin and tannins, were selected for investigations. We show that such end-product carbon characteristic as functional and elemental composition, porosity, specific surface area, defectiveness level, and morphology strictly depend on the precursor composition, chemical structure, and pyrolysis temperature. The electrochemical characteristics of produced carbon materials correlate with the characteristics of the produced materials. A higher pyrolysis temperature is shown to be favourable for production of carbon material for the Li-ion battery application in terms of both specific capacity and long-term cycling stability.
PubMed: 38126627
DOI: 10.1002/cssc.202301005 -
Chemical Communications (Cambridge,... May 2024Caged -dithiols have been developed as the donors of reactive sulfur species (RSS), but the chemistry of free -dithiols as RSS donors has not been well understood....
Caged -dithiols have been developed as the donors of reactive sulfur species (RSS), but the chemistry of free -dithiols as RSS donors has not been well understood. Herein, we report the study of a free -dithiol, 1,3-diphenylpropane-2,2-dithiol, as the precursor for several RSS. It releases HS under physiological conditions and can be converted to a mono--nitrosothiol, which serves as a NO donor. Furthermore, it can be converted to 3,3-dibenzyldithiirane, which is an active sulfur transfer reagent and can induce -persulfidation.
PubMed: 38712962
DOI: 10.1039/d4cc01003e -
BioRxiv : the Preprint Server For... Apr 2024Hematopoietic dysfunction has been associated with a reduction in the number of active precursors. However, precursor quantification at homeostasis and under diseased...
Hematopoietic dysfunction has been associated with a reduction in the number of active precursors. However, precursor quantification at homeostasis and under diseased conditions is constrained by the scarcity of available methods. To address this issue, we optimized a method for quantifying a wide range of hematopoietic precursors. Assuming the random induction of a stable label in precursors following a binomial distribution, the estimation depends on the inverse correlation between precursor numbers and the variance of precursor labeling among independent samples. Experimentally validated to cover the full dynamic range of hematopoietic precursors in mice (1 to 10), we utilized this approach to demonstrate that thousands of precursors, which emerge after modest expansion during fetal-to-adult transition, contribute to native and perturbed hematopoiesis. We further estimated the number of precursors in a mouse model of Fanconi Anemia, showcasing how repopulation deficits can be segregated into autologous (cell proliferation) and non-autologous causes (lack of precursor). Our results support an accessible and reliable approach for precursor quantification, emphasizing the contemporary perspective that native hematopoiesis is highly polyclonal.
PubMed: 38617223
DOI: 10.1101/2024.04.02.587737 -
Environmental Science. Nano Mar 2024Photocatalyst synthesis typically involves multiple steps, expensive precursors, and solvents. In contrast, spark ablation offers a simple process of electrical...
Photocatalyst synthesis typically involves multiple steps, expensive precursors, and solvents. In contrast, spark ablation offers a simple process of electrical discharges in a gap between two electrodes made from a desirable material. This enables a precursor- and waste-free generation of pure metal oxide nanoparticles or mixtures of various compositions. This study presents a two-step method for the production of photocatalytic filters with deposited airborne MnO, TiO, and ZnO nanoparticles using spark ablation and calcination processes. The resulting MnO and TiO filters demonstrated almost twice the activity with outstanding performance stability, as compared to sol-gel MnO and commercial TiO. The introduced method is not only simple, precursor- and waste-free, and leads to superior performance for the case studied, but it also has future potential due to its versatility. It can easily produce mixed and doped materials with further improved properties, making it an interesting avenue for future research.
PubMed: 38496350
DOI: 10.1039/d3en00348e -
Amino Acids Oct 2023Cancer malignancies may broadly be described as heterogeneous disorders manifested by uncontrolled cellular growth/division and proliferation. Tumor cells utilize... (Review)
Review
Cancer malignancies may broadly be described as heterogeneous disorders manifested by uncontrolled cellular growth/division and proliferation. Tumor cells utilize metabolic reprogramming to accomplish the upregulated nutritional requirements for sustaining their uncontrolled growth, proliferation, and survival. Metabolic reprogramming also called altered or dysregulated metabolism undergoes modification in normal metabolic pathways for anabolic precursor's generation that serves to continue biomass formation that sustains the growth, proliferation, and survival of carcinogenic cells under a nutrition-deprived microenvironment. A wide range of dysregulated/altered metabolic pathways encompassing different metabolic regulators have been described; however, the current review is focused to explain deeply the metabolic pathways modifications inducing upregulation of proteins/amino acids metabolism. The essential modification of various metabolic cycles with their consequent outcomes meanwhile explored promising therapeutic targets playing a pivotal role in metabolic regulation and is successfully employed for effective target-specific cancer treatment. The current review is aimed to understand the metabolic reprogramming of different proteins/amino acids involved in tumor progression along with potential therapeutic perspective elucidating targeted cancer therapy via these targets.
Topics: Humans; Neoplasms; Metabolic Networks and Pathways; Amino Acids; Tumor Microenvironment
PubMed: 37646877
DOI: 10.1007/s00726-023-03316-y -
Journal of Colloid and Interface Science Dec 2023Cysteine, as a non-aromatic precursor, was used to produce Nitrogen (N) and Sulfur (S) sources for preparing N, S-doped carbon dots (CDs) with tunable luminescence...
Cysteine, as a non-aromatic precursor, was used to produce Nitrogen (N) and Sulfur (S) sources for preparing N, S-doped carbon dots (CDs) with tunable luminescence emission. Despite the tremendous investigations, the photoluminescence (PL) mechanism of CDs is still unclear due to its complex core-shell structure, variety of surface functional groups, and structure dependency. This study focuses on controlling aromatization and graphitization processes during the hydrothermal synthesis on CDs by using Citric Acid (CA) and Ammonium persulfate. Detailed characterizations by FTIR spectroscopy, XPS, and HR-TEM are provided to suggest both chemical and bandgap structures. Results reveal that the red-shift of PL occurred due to the graphitization and increasing content of graphitic nitrogen in the core, as well as the Pyridinic and Amine groups creating sub-bands on the surface. These findings resolve the controversy on the PL mechanism of Cysteine-based CDs and provide a general guide for increasing the aromatization and graphitization degree from non-aromatic precursors which clarify the mechanism exploration and structural analysis of other types of CDs.
PubMed: 37540928
DOI: 10.1016/j.jcis.2023.07.157 -
Chemosphere Sep 2023To understand sources and processes affecting per- and polyfluoroalkyl substances (PFAS), 32 PFAS were measured in landfill leachate from 17 landfills across Washington...
To understand sources and processes affecting per- and polyfluoroalkyl substances (PFAS), 32 PFAS were measured in landfill leachate from 17 landfills across Washington State in both pre-and post-total oxidizable precursor (TOP) assay samples, using an analytical method that was the precursor to EPA Draft Method 1633. As in other studies, 5:3FTCA was the dominant PFAS in the leachate, suggesting that carpets, textiles, and food packaging were the main sources of PFAS. Total PFAS concentrations (ΣPFAS) ranged from 61 to 172,976 ng/L and 580-36,122 ng/L in pre-TOP and post-TOP samples, respectively, suggesting that little or no uncharacterized precursors remained in landfill leachate. Furthermore, due to chain-shortening reactions, the TOP assay often resulted in a loss of overall PFAS mass. Positive matrix factorization (PMF) analysis of the combined pre- and post-TOP samples produced five factors that represent sources and processes. Factor 1 consisted primarily of 5:3FTCA (intermediate of 6:2 fluorotelomer degradation and characteristic of landfill leachate), while factor 2 was dominated by PFBS (degradant of C-4 sulfonamide chemistry) and, to a lesser extent, by several PFCAs and 5:3FTCA. Factor 3 consisted primarily of both short-chain PFCAs (end-products of 6:2 fluorotelomer degradation) and PFHxS (derived from C-6 sulfonamide chemistry), while the main component of factor 4 was PFOS (dominant in many environmental media but minor in landfill leachate, perhaps reflecting a production shift from longer to shorter chain PFAS). Factor 5, highly loaded with PFCAs, was dominant in post-TOP samples and therefore represented the oxidation of precursors. Overall, PMF analysis suggests that the TOP assay approximates some redox processes which occur in landfills, including chain-shortening reactions which yield biodegradable products.
Topics: Water Pollutants, Chemical; Fluorocarbons; Waste Disposal Facilities; Washington
PubMed: 37209854
DOI: 10.1016/j.chemosphere.2023.138924