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Veterinary Medicine and Science Jul 2023Pericarditis means inflammation in the pericardial sac. Pericarditis is divided into three categories based on morphology, including fibrinous, purulent, and...
Pericarditis means inflammation in the pericardial sac. Pericarditis is divided into three categories based on morphology, including fibrinous, purulent, and constrictive. In the present study, a 7-month-old male Ghezel breed sheep was examined for low weighting rate for three months. Tachypnea, tachycardia, heart friction sound, absence of fever and normal appetite were recorded in the clinical examination. In the patient's history, there was a history of perforated chest trauma behind the left scapula about three months ago. After the echocardiography examination and bacteriology procedures, purulent pericarditis caused by Proteus mirabilis was diagnosed. The bacterium was analysed using genome sequencing and new strain called Abhar114 was diagnosed. This is the first report of pericarditis caused by Proteus mirabilis in sheep.
Topics: Male; Animals; Sheep; Proteus mirabilis; Pericarditis; Echocardiography; Sheep Diseases
PubMed: 37192480
DOI: 10.1002/vms3.1160 -
SAGE Open Medicine 2023This study aimed to evaluate the microbiological quality of water sources in Ishaka division, Bushenyi district.
OBJECTIVES
This study aimed to evaluate the microbiological quality of water sources in Ishaka division, Bushenyi district.
METHODS
Water from taps, wells and springs were sampled for the cross-sectional investigation. The enumeration and identification of microbes ( and total coliforms) in water samples were carried out using a variety of methods. was enumerated using the membrane filtration method; and using a two-step enrichment method; using the surface spread method and total coliforms using the most probable number technique. Mannitol salt agar was used for enumeration of and violet red bile agar was used for enumeration of total coliforms and ; xylose lysine deoxycholate agar was used for both spp. and spp. API-20E was used to phenotypically identify the Enterobacteriaceae contaminants in water. These included spp. and .
RESULTS
counts in the water from springs and wells ranged from 0 to 314 cfu/mL ( = 0.173) and 0 to 3 cfu/mL ( = 0.269), respectively, while tap water had no incidence of . Highest level of bacterial contamination in water sources, beyond acceptable WHO (0 cfu/100 mL) limits for drinking water, was reported: spp., 34 (54.8%), followed by total coliforms, 24 (38.7%), spp., 22 (35.5%) and least were spp. (8.1%) and spp. (8.1%).
CONCLUSION
It is therefore concluded that spring and well community water sources in Ishaka division, Uganda, are significantly contaminated with pathogenic bacteria and thus unsafe for drinking without adequate water treatment (disinfection and filtration).
PubMed: 38020800
DOI: 10.1177/20503121231194239 -
Annals of Clinical Microbiology and... May 2024Proteus mirabilis is a significant nosocomial pathogen that is frequently associated with a wide range of infections, necessitating heightened attention to mitigate...
BACKGROUND
Proteus mirabilis is a significant nosocomial pathogen that is frequently associated with a wide range of infections, necessitating heightened attention to mitigate potential health risks. Hence, this study was performed to investigate the impact of sub-minimum inhibitory concentrations (MICs) of ciprofloxacin (CIP) on Proteus mirabilis clinical isolates.
METHODS
The sub-MICs of CIP were selected using the growth curve approach. The untreated and treated isolates with sub-MICs of CIP were assessed for their biofilm development, motilities on agar, and other virulence factors. The cell morphology of untreated and treated isolates with sub-MIC of CIP was explored using electron microscope. Moreover, the expression levels of the virulence genes in isolates were measured using quantitative real-time PCR.
RESULTS
Data revealed that sub-MICs of CIP significantly (p < 0.05), in a concentration-dependent manner, inhibited biofilm formation and other virulence factors in the selected isolates. Electron microscope analysis showed cell enlargement and various abnormalities in the cell wall and membrane integrity.
CONCLUSION
Sub-MICs of CIP exhibited inhibition of virulence and alterations in morphological integrity against P. mirabilis isolates.
Topics: Proteus mirabilis; Ciprofloxacin; Biofilms; Microbial Sensitivity Tests; Humans; Anti-Bacterial Agents; Proteus Infections; Virulence Factors; Virulence
PubMed: 38802894
DOI: 10.1186/s12941-024-00704-4 -
Biometals : An International Journal on... May 20242-((1-(4-((2,4,6-trioxohexahydropyrimidin-5-yl)diazenyl) phenyl) ethylidene) amino) benzoic acid (HL), and its V(IV), Co(II), Ni(II), Cu(II), Pd(II) and Ag(I) chelates...
2-((1-(4-((2,4,6-trioxohexahydropyrimidin-5-yl)diazenyl) phenyl) ethylidene) amino) benzoic acid (HL), and its V(IV), Co(II), Ni(II), Cu(II), Pd(II) and Ag(I) chelates were synthesized. They were defined using multiple spectral and analytical techniques. With the exception of Ag(I) chelate, all chelates possessed non-electrolytic character. Square pyramidal shape was proposed for V(IV) chelate and Square planar for the other chelates. The analysis of functional group bands of HL and its coordination compounds alludes that HL chelated as neutral tetradentate via nitrogen atoms of azo and azomethine groups, oxygen atom of carbonyl of barbituric acid and OH of the carboxylic group. TG/DTG predicted the thermal behaviors of all compounds. The antibacterial activity of HL and its coordination compounds was conducted against Proteus mirabilis at concentrations of 250, 500, and 1000 µg/mL. Ag(I) at 1000 µg/mL, showed the most inhibiting potency against P. mirabilis and registered zone of inhibition of 28.33 ± 0.84 mm and highest biofilm inhibition of 70.31%. At 50 Gy of gamma irradiation, the reducing effect of Ag(I) chelate was improved. The protein interruption of P. mirabilis was greatly interrupted by increasing the concentration of the chaletes. Also, Ag(I) showed the highest cytotoxicity with IC value of 11.5 µg/ mL. The novelty of this study is the synthesis of a new azo-Schiff base and this is almost the first publication of the effect of azo-Schiff ligands against that bacterial strain P. mirabilis.
PubMed: 38811521
DOI: 10.1007/s10534-024-00608-y -
Experimental and Therapeutic Medicine Sep 2023is a common pathogen, which is responsible for urinary tract infections. Iron is a critical element necessary for both humans and pathogens to maintain their biological...
is a common pathogen, which is responsible for urinary tract infections. Iron is a critical element necessary for both humans and pathogens to maintain their biological functions, and iron limitation via chelator agents may be useful in the treatment of infections. The present study aimed to investigate the synergistic interactions between the iron chelator agent deferoxamine (DFO) and the antibacterial drug colistin. The minimum inhibitory concentration (MIC) values of DFO and colistin for isolates were determined by broth microdilution. The checkerboard technique was used to examine the potential synergy between DFO and colistin. Furthermore, time-kill assays were used for the confirmation of synergy detected by the checkerboard assay, as well as for determining bacteriostatic and bactericidal interactions throughout a 24-h period. As expected, all isolates were resistant to colistin. DFO did not inhibit growth when used alone, even at very high doses (10 µg ml). Notably, when in combination with DFO, the MIC values of colistin were markedly reduced, and the checkerboard assay results showed synergy between colistin and DFO for all isolates. In addition, in time-kill assays, colistin + DFO exhibited synergistic activity against all strains at most time intervals and concentrations tested. Colistin + DFO showed bactericidal activity at colistin concentrations of 1xMIC and 2xMIC, although a degree of re-growth was observed in one of the strains at 12-24 h. These findings indicated that DFO has the potential for use as an adjunct to colistin through iron sequestration, thus providing synergistic activity to an antibiotic that would not normally be considered a treatment option against experiments in the future may provide useful information on the efficacy of DFO/colistin since these models effectively reflect physiological parameters.
PubMed: 37614438
DOI: 10.3892/etm.2023.12158 -
Pathogens (Basel, Switzerland) Nov 2023is a common uropathogen and a leading cause of catheter-associated urinary tract infections (CAUTIs), which are often polymicrobial. Through a genome-wide screen, we...
is a common uropathogen and a leading cause of catheter-associated urinary tract infections (CAUTIs), which are often polymicrobial. Through a genome-wide screen, we previously identified two [NiFe] hydrogenases as candidate fitness factors for CAUTI: a Hyb-type Group 1c H-uptake hydrogenase and a Hyf-type Group 4a H-producing hydrogenase. In this study, we disrupted one gene of each system ( and ) and also generated a double mutant to examine the contribution of flexible H metabolism to growth and fitness in vitro and during experimental CAUTI. Since is typically present as part of a polymicrobial community in the urinary tract, we also examined the impact of two common co-colonization partners, and , on the expression and contribution of each hydrogenase to fitness. Our data demonstrate that neither system alone is critical for growth in vitro or fitness during experimental CAUTI. However, perturbation of flexible H metabolism in the ∆∆ double mutant decreased fitness in vitro and during infection. The Hyf system alone contributed to the generation of proton motive force and swarming motility, but only during anaerobic conditions. Unexpectedly, both systems contributed to benzyl viologen reduction in TYET medium, and disruption of either system increased expression of the other. We further demonstrate that polymicrobial interactions with and alter the expression of Hyb and Hyf in vitro as well as the contribution of each system to fitness during CAUTI.
PubMed: 38133262
DOI: 10.3390/pathogens12121377 -
Sensors (Basel, Switzerland) Sep 2023Bacterial resistance to antibiotics is a primary global healthcare concern as it hampers the effectiveness of commonly used antibiotics used to treat infectious...
Bacterial resistance to antibiotics is a primary global healthcare concern as it hampers the effectiveness of commonly used antibiotics used to treat infectious diseases. The development of bacterial resistance continues to escalate over time. Rapid identification of the infecting bacterium and determination of its antibiotic susceptibility are crucial for optimal treatment and can save lives in many cases. Classical methods for determining bacterial susceptibility take at least 48 h, leading physicians to resort to empirical antibiotic treatment based on their experience. This random and excessive use of antibiotics is one of the most significant drivers of the development of multidrug-resistant (MDR) bacteria, posing a severe threat to global healthcare. To address these challenges, considerable efforts are underway to reduce the testing time of taxonomic classification of the infecting bacterium at the species level and its antibiotic susceptibility determination. Infrared spectroscopy is considered a rapid and reliable method for detecting minor molecular changes in cells. Thus, the main goal of this study was the use of infrared spectroscopy to shorten the identification and the susceptibility testing time of and from 48 h to approximately 40 min, directly from patients' urine samples. It was possible to identify the and species with 99% accuracy and, simultaneously, to determine their susceptibility to different antibiotics with an accuracy exceeding 80%.
Topics: Humans; Pseudomonas; Microbial Sensitivity Tests; Proteus; Bacteria; Bacterial Infections; Anti-Bacterial Agents; Spectrophotometry, Infrared; Machine Learning; Urinary Tract Infections
PubMed: 37836961
DOI: 10.3390/s23198132 -
Brazilian Journal of Microbiology :... Sep 2023Treatment of Proteus mirabilis infections is a challenge due to the high abundance of virulence factors and the high intrinsic resistance to antimicrobials. Multidrug...
Treatment of Proteus mirabilis infections is a challenge due to the high abundance of virulence factors and the high intrinsic resistance to antimicrobials. Multidrug resistance (MDR) and extensive drug resistance (XDR) further challenge the control of P. mirabilis infection. This study aimed to investigate the correlation between virulence determinants and multidrug resistance in 100 clinical isolates of P. mirabilis collected in Alexandria from December 2019 to June 2021. Susceptibility to antimicrobials was tested by the Kirby Bauer method. Detection of swarming, urease, protease, hemolysin, and biofilm formation was performed phenotypically and by PCR amplification of zapA, flaA, ureC, mrpA, atfA, ucaA, hpmA, and luxS. MDR and XDR were detected in 34% and 5%, respectively. All isolates were positive for motility, swarming, urease, and protease production. Ninety percent were positive for hemolysin production, while 73% formed biofilm. All isolates possessed the ureC and zapA genes. The luxS, flaA, ucaA, hpmA, mrpA, and atfA genes were detected in 99%, 98%, 96% 90%, 89%, and 84%, respectively. The presence of a single biofilm-related gene was statistically correlated with non-biofilm production (P= 0.018). It was concluded that P. mirabilis isolates from catheterized-urine samples were significantly associated with biofilm formation. MDR and virulence were not statistically correlated. A significant positive correlation was detected between some virulence genes in P. mirabilis. Non-MDR isolates of P. mirabilis had a high abundance of virulence factors with no statistically significant difference from MDR. Most of the MDR and all XDR isolates could produce biofilm.
Topics: Virulence Factors; Proteus mirabilis; Hemolysin Proteins; Urease; Correlation of Data; Drug Resistance, Multiple; Peptide Hydrolases; Biofilms; Anti-Bacterial Agents
PubMed: 37535261
DOI: 10.1007/s42770-023-01080-5 -
Biomaterial Investigations in Dentistry 2023The aim of this study was the development of a complex multispecies endodontic biofilm using , and on a biofilm of in a dentinal substrate design. The endodontic...
The aim of this study was the development of a complex multispecies endodontic biofilm using , and on a biofilm of in a dentinal substrate design. The endodontic pathology is a biofilm-mediated infection, and the aim of root canal therapy is to reduce, as much as possible, the bacterial population. Thus, it is important to develop a laboratory endodontic biofilm to test the effect of new irrigation and obturation techniques on reduction of bacterial count. The culture of from ATCC 29212 began with aerobic cultivation on blood agar, followed by transfer to Brain Heart Infusion (BHI) broth with 5% sucrose. Incubation occurred in a shaker at 37 °C for 24 h, followed by an additional 24-h static phase. After 10 d, , , and Candida albicans were introduced sequentially in three distinct groups. Group 1: the order of addition was , , and ; Group 2: the order was , , and ; and Group 3: , , and After 16 days, the biofilm was carefully extracted, transferred to sterile BHI, and dissected using a sterile needle technique. Subsequently, an optical density test, bacterial counts, and colony enumeration were performed on various agar plates. Group 2 in which was added directly after followed and showed significantly greater total bacterial count than the other two groups.
PubMed: 38204478
DOI: 10.1080/26415275.2023.2281091 -
Infectious Diseases and Therapy Jul 2023Gram-negative resistance is a well-acknowledged public health threat. Surveillance data can be used to monitor resistance trends and identify strategies to mitigate...
INTRODUCTION
Gram-negative resistance is a well-acknowledged public health threat. Surveillance data can be used to monitor resistance trends and identify strategies to mitigate their threat. The objective of this study was to assess antibiotic resistance trends in Gram-negative bacteria.
METHODS
The first cultures of Pseudomonas aeruginosa, Citrobacter, Escherichia coli, Enterobacter, Klebsiella, Morganella morganii, Proteus mirabilis, and Serratia marcescens per hospitalized patient per month collected from 125 Veterans Affairs Medical Centers (VAMCs) between 2011 to 2020 were included. Time trends of resistance phenotypes (carbapenem, fluoroquinolone, extended-spectrum cephalosporin, multi-drug, and difficult-to-treat) were analyzed with Joinpoint regression to estimate average annual percent changes (AAPC) with 95% confidence intervals and p values. A 2020 antibiogram of reported antibiotic percent susceptibilities was also created to evaluate resistance rates at the beginning of the COVID-19 pandemic.
RESULTS
Among 40 antimicrobial resistance phenotype trends assessed in 494,593 Gram-negative isolates, there were no noted increases; significant decreases were observed in 87.5% (n = 35), including in all P. aeruginosa, Citrobacter, Klebsiella, M. morganii, and S. marcescens phenotypes (p < 0.05). The largest decreases were seen in carbapenem-resistant phenotypes of P. mirabilis, Klebsiella, and M. morganii (AAPCs: - 22.9%, - 20.7%, and - 20.6%, respectively). In 2020, percent susceptibility was over 80% for all organisms tested against aminoglycosides, cefepime, ertapenem, meropenem, ceftazidime-avibactam, ceftolozane-tazobactam, and meropenem-vaborbactam.
CONCLUSION
We observed significant decreases in antibiotic resistance for P. aeruginosa and Enterobacterales over the past decade. According to the 2020 antibiogram, in vitro antimicrobial activity was observed for most treatment options. These results may be related to the robust infection control and antimicrobial stewardship programs instituted nationally among VAMCs.
PubMed: 37326931
DOI: 10.1007/s40121-023-00827-9