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BMC Infectious Diseases Dec 2023Pseudomonas otitidis belongs to the genus Pseudomonas and causes various infections, including ear, skin, and soft tissue infections. P. otitidis has a unique... (Review)
Review
BACKGROUND
Pseudomonas otitidis belongs to the genus Pseudomonas and causes various infections, including ear, skin, and soft tissue infections. P. otitidis has a unique susceptibility profile, being susceptible to penicillins and cephalosporins but resistant to carbapenems, due to the production of the metallo-β-lactamase called POM-1. This revealed genetic similarities with Pseudomonas aeruginosa, which can sometimes lead to misidentification.
CASE PRESENTATION
We report the case of a 70-year-old Japanese male who developed cellulitis and bacteremia during chemotherapy for multiple myeloma. He was initially treated with meropenem, but blood culture later revealed gram-negative bacilli identified as P. otitidis using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Carbapenem resistance was predicted from previous reports; therefore, we switched to dual therapy with levofloxacin and cefepime, and favorable treatment results were obtained.
CONCLUSION
This is the first reported case of P. otitidis cellulitis and bacteremia in an immunocompromised patient. Carbapenems are typically used in immunocompromised patients and P. otitidis is often resistant to it. However, its biochemical properties are similar to those of Pseudomonas aeruginosa; therefore, its accurate identification is critical. In the present study, we rapidly identified P. otitidis using MALDI-TOF MS and switched from carbapenems to an appropriate antimicrobial therapy, resulting in a successful outcome.
Topics: Humans; Male; Aged; Anti-Bacterial Agents; Cellulitis; Pseudomonas; Carbapenems; Pseudomonas Infections; Pseudomonas aeruginosa; Bacteremia; Immunocompromised Host; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
PubMed: 38110897
DOI: 10.1186/s12879-023-08919-0 -
Letters in Applied Microbiology Jun 2024This study explores the eco-friendly synthesis of silver nanoparticles (AgNPs) using soil bacteria, Pseudomonas otitidis. The bio-synthesized AgNPs were characterized...
This study explores the eco-friendly synthesis of silver nanoparticles (AgNPs) using soil bacteria, Pseudomonas otitidis. The bio-synthesized AgNPs were characterized using various techniques, including UV-visible spectroscopy, Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and X-ray diffraction (XRD). UV-visible spectroscopy revealed a distinct broad absorption band in the range of 443 nm, indicating the reduction of silver nitrate to AgNPs. XRD analysis provided evidence of the crystalline nature of the particles, with sharp peaks confirming their crystallinity and an average size of 82.76 nm. FTIR spectroscopy identified extracellular protein compounds as capping agents. SEM examination revealed spherical agglomeration of the crystalline AgNPs. The antimicrobial assay by a disc diffusion method, minimum inhibitory concentration, and minimum bactericidal concentration testing revealed that the biosynthesized AgNPs showed moderate antibacterial activity against both pathogenic Gram-negative (Klebsiella pneumoniae, Pseudomonas aeruginosa, and Acinetobacter baumannii) and Gram-positive (Bacillus cereus, Staphylococcus aureus, and Streptococcus mutans) bacterial strains. Furthermore, the AgNPs significantly disrupted the biofilm of P. aeruginosa, as confirmed by crystal violet assay and fluorescent microscopy. Overall, this study underscores the potential of microbial-synthesized nanoparticles in biomedical applications, particularly in combating pathogenic bacteria, offering a promising avenue for future research and development.
Topics: Silver; Biofilms; Metal Nanoparticles; Anti-Bacterial Agents; Microbial Sensitivity Tests; Pseudomonas; Spectroscopy, Fourier Transform Infrared; X-Ray Diffraction; Pseudomonas aeruginosa
PubMed: 38845375
DOI: 10.1093/lambio/ovae053 -
Journal of Global Antimicrobial... Mar 2024The contamination of fresh surface waters poses a significant burden on human health and prosperity, especially in marginalized communities with limited resources and...
OBJECTIVES
The contamination of fresh surface waters poses a significant burden on human health and prosperity, especially in marginalized communities with limited resources and inadequate infrastructure. Here, we performed in-depth genomic analyses of multidrug-resistant bacteria (MDR-B) isolated from Al-Oueik river water that is used for irrigation of agricultural fields in a disenfranchised area that also hosts a makeshift Syrian refugee camp.
METHODS
A composite freshwater sample was filtered. Faecal coliforms were counted and extended spectrum cephalosporins and/or ertapenem resistant bacteria were screened. Isolates were identified using MALDI-TOF-MS and analysed using whole-genome sequencing (WGS) to identify the resistome, sequence types, plasmid types, and virulence genes.
RESULTS
Approximately 10 CFU/100 mL of faecal coliforms were detected in the water. Four drug-resistant Gram-negative bacteria were identified, namely Escherichia coli, Klebsiella pneumoniae, Enterobacter hormaechei, and Pseudomonas otitidis. Notably, the E. coli isolate harboured bla and a YRIN-inserted PBP3, representing an emerging public health challenge. The K. pneumoniae isolate carried bla as well as mutations in the gene encoding the OmpK37 porin. Enterobacter hormaechei and P. otitidis harboured bla and bla, respectively.
CONCLUSION
This report provides comprehensive genomic analyses of MDR-B in irrigation water in Lebanon. Our results further support that irrigation water contaminated with faecal material can be a reservoir of important MDR-B, which can spread to adjacent agricultural fields and other water bodies, posing both public health and food safety issues. Therefore, there is an urgent need to implement effective water quality monitoring and management programs to control the proliferation of antibiotic-resistant pathogens in irrigation water in Lebanon.
Topics: Humans; Escherichia coli; Rivers; Enterobacter; Plasmids; Klebsiella pneumoniae; Gram-Negative Bacteria
PubMed: 38154747
DOI: 10.1016/j.jgar.2023.12.016 -
Biologia Futura Sep 2023To study how biofilm-forming rhizobacteria isolated from mines and dumpsites improved the phytoremediation efficacy of B. juncea in metal-contaminated soil. Out of 91...
Biofilm-forming plant growth-promoting rhizobacterial consortia isolated from mines and dumpsites assist green remediation of toxic metal (Ni and Pb) using Brassica juncea.
To study how biofilm-forming rhizobacteria isolated from mines and dumpsites improved the phytoremediation efficacy of B. juncea in metal-contaminated soil. Out of 91 isolates, six were chosen for research based on their tolerance to metals, and their efficient PGPR properties, and subjected to the design of a consortium. A compatibility study revealed no antagonistic interaction between rhizobacterial-consortiums. The results of the biofilm formation and FEG-SEM studies revealed that a consortium-BC8 formed a strong biofilm on the root surface of B. juncea seedlings. Based on results obtained with the phytoextraction efficiency of B. juncea in consortium-BC8 (SMHMZ46 and SMHMP23), they were identified as Klebsiella variicola and Pseudomonas otitidis, respectively, and submitted to NCBI GenBank with accession numbers MZ145092 and OK560623. This rhizobacteria is the first to be reported as assisting Ni and Pb phytoremediation by employing B. juncea. Soil inoculation with consortium-BC8 increased the amount of soluble Ni and Pb by 13.25-fold and 10.69-fold, respectively, when compared to the control. These consortiums-BC8 significantly increased vegetative growth and metal accumulation in root and shoot with a translocation-factor of 1.58 for Ni and soil to root with a bioconcentration-factor of 1.3 for Pb in B. juncea grown in individual soil contamination with 96.05 mg/kg NiCl and 89.63 mg/kg Pb(NO), which are significantly higher than other consortium treatments and the non-inoculated control. B. juncea amendments with a biofilm-forming consortium-BC8 having TF, BCF, and BAC > 1 for Ni, whereas BCF > 1, TF, and BAC < 1 for Pb, are appropriate for green remediation of Ni and phytostabilization of Pb.
PubMed: 37733193
DOI: 10.1007/s42977-023-00179-y -
Microbiology Resource Announcements Mar 2024Here, we report the draft genome sequences of strains zfem001-005, five isolates from the intestinal microbiota of healthy larval zebrafish at a developmental age of 7...
Here, we report the draft genome sequences of strains zfem001-005, five isolates from the intestinal microbiota of healthy larval zebrafish at a developmental age of 7 days post fertilization. The isolates have been identified as , , , , and , respectively.
PubMed: 38376340
DOI: 10.1128/mra.00934-23