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Journal of Medicinal Chemistry May 2024The novel metal(II)-based complexes HA-Cu, HA-Co, and HA-Ni with phenanthroline, sulfamethazine, and aromatic-aromatic coupled disulfamethazines as ligands were...
Arene-Arene Coupled Disulfamethazines (or Sulfadiazine)-Phenanthroline-Metal(II) Complexes were Synthesized by In Situ Reactions and Inhibited the Growth and Development of Triple-Negative Breast Cancer through the Synergistic Effect of Antiangiogenesis, Anti-Inflammation, Pro-Apoptosis, and...
The novel metal(II)-based complexes HA-Cu, HA-Co, and HA-Ni with phenanthroline, sulfamethazine, and aromatic-aromatic coupled disulfamethazines as ligands were synthesized and characterized. HA-Cu, HA-Co, and HA-Ni all showed a broad spectrum of cytotoxicity and antiangiogenesis. HA-Cu was superior to HA-Co and HA-Ni, and even superior to DDP, showing significant inhibitory effect on the growth and development of tripe-negative breast cancer in vivo and in vitro. HA-Cu exhibited observable synergistic effects of antiproliferation, antiangiogenesis, anti-inflammatory, pro-apoptosis, and cuproptosis to effectively inhibited tumor survival and development. The molecular mechanism was confirmed that HA-Cu could downregulate the expression of key proteins in the VEGF/VEGFR2 signaling pathway and the expression of inflammatory cytokines, enhance the advantage of pro-apoptotic protein Bax, and enforce cuproptosis by weakening the expression of FDX1 and enhancing the expression of HSP70. Our research will provide a theoretical and practical reference for the development of metal-sulfamethazine and its derivatives as chemotherapy drugs for cancer treatment.
Topics: Apoptosis; Humans; Coordination Complexes; Animals; Phenanthrolines; Female; Triple Negative Breast Neoplasms; Angiogenesis Inhibitors; Antineoplastic Agents; Cell Proliferation; Mice; Cell Line, Tumor; Anti-Inflammatory Agents; Drug Synergism; Structure-Activity Relationship; Mice, Inbred BALB C; Drug Screening Assays, Antitumor
PubMed: 38634624
DOI: 10.1021/acs.jmedchem.3c02432 -
Military Medicine Sep 2023Immediate evacuation of burn casualties can be challenging in austere environments, and it is predicted to be even more difficult in future multi-domain battlespaces...
INTRODUCTION
Immediate evacuation of burn casualties can be challenging in austere environments, and it is predicted to be even more difficult in future multi-domain battlespaces against near-peer foes. Therefore, a need exists to treat burn wounds at the point of injury to protect the exposed injury for an extended period. In this study, we compare two commercially available FDA-approved therapies to the current gold standard of care (GSOC), excisional debridement followed by the application of split-thickness skin graft, and the standard for prolonged field care, silver sulfadiazine (SSD) cream. The shelf-stable therapies evaluated were irradiated human skin (IHS) allograft and polylactic acid (PLA). Our objective was to study whether they have the potential capability to reduce the need for evacuation to a burn center for surgical intervention so that the combat power can be preserved in the field.
MATERIALS AND METHODS
Sixteen burns (50 cm2) were created on the dorsum of four anesthetized swine. All materials were sterile, but a sterile field was not utilized in order to simulate the prolonged field care setting. The wounds were then treated with PLA, IHS, and SSD cream, and the remaining wounds (designated GSOC) were also treated with SSD cream. On post-operative day (POD) 3, sterile surgical debridement and skin grafting (1:4) were performed on the GSOC wounds. Burn healing was followed for either PODs 10, 14, 21, or 28, wherein one animal was humanely euthanized at each time point; each represented a time point of the healing process. A full-thickness excisional biopsy was taken from each wound immediately after euthanasia to give a cross-section view of the wound edge to edge. Wound healing was determined by the histological analysis of wound re-epithelialization, epidermal thickness, rete ridges, and scar elevation index and macroscopically using noninvasive imaging systems.
RESULTS
The PLA and IHS treatments did not need to be reapplied to the wounds during the course of the experiment, unlike SSD, which was reapplied at each assessment time point. In terms of re-epithelialization, on POD 10, IHS and SSD were similar to the GSOC; on POD 14, all treatments were similar; on POD 21, PLA and IHS were similar to SSD; finally, on POD 28, re-epithelialization was similar in all groups. On POD 28, scar elevation index and rete ridges/mm were similar to all groups, and epidermal and dermal thickness for PLA and IHS were similar to GSOC.
CONCLUSIONS
This preclinical study demonstrated that the use of the PLA and the IHS dressings resulted in similar outcomes to the GSOC-treated burns in several key metrics of wound healing. These therapies represent a potentially useful tool in current and future battlespaces, where surgical intervention is not possible. The products are lightweight and, more importantly, stable at room temperature for their entire shelf lives. This would allow for easy storage and transport by medical practitioners in the field.
PubMed: 37776542
DOI: 10.1093/milmed/usad390 -
Journal of Environmental Sciences... Aug 2023Antibiotic pollution has become a global eco-environmental issue. To reduce sulfonamide antibiotics in water and improve resource utilization of solid wastes,...
Antibiotic pollution has become a global eco-environmental issue. To reduce sulfonamide antibiotics in water and improve resource utilization of solid wastes, phosphogypsum modified biochar composite (PMBC) was prepared via facile one-step from distillers grains, wood chips, and phosphogypsum. The physicochemical properties of PMBC were characterized by scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR), Zeta potential, X-ray diffraction (XRD), etc. The influencing factors, adsorption behaviors, and mechanisms of sulfadiazine (SD) and sulfamethazine (SMT) onto PMBC were studied by batch and fixed bed column adsorption experiments. The results showed that the removal rates of SD and SMT increased with the increase of phosphogypsum proportion, while decreased with the increase of solution pH. The maximum adsorption capacities of modified distillers grain and wood chips biochars for SD were 2.98 and 4.18 mg/g, and for SMT were 4.40 and 8.91 mg/g, respectively, which was 9.0-22.3 times that of pristine biochar. Fixed bed column results demonstrated that PMBC had good adsorption capacities for SD and SMT. When the solution flow rate was 2.0 mL/min and the dosage of PMBC was 5.0 g, the removal rates of SD and SMT by modified wood chips biochar were both higher than 50% in 4 hr. The main mechanisms of SD and SMT removal by PMBC are hydrogen bonding, π-π donor-acceptor, electrostatic interaction, and hydrophobic interaction. This study provides an effective method for the removal of antibiotics in water and the resource utilization of phosphogypsum.
Topics: Anti-Bacterial Agents; Water; Water Pollutants, Chemical; Charcoal; Sulfanilamide; Sulfamethazine; Sulfonamides; Sulfadiazine; Adsorption; Spectroscopy, Fourier Transform Infrared; Kinetics
PubMed: 37032034
DOI: 10.1016/j.jes.2022.10.023 -
Journal of Nanobiotechnology Sep 2023Wounds can lead to skin and soft tissue damage and their improper management may lead to the growth of pathogenic bacteria at the site of injury. Identifying better ways...
Wounds can lead to skin and soft tissue damage and their improper management may lead to the growth of pathogenic bacteria at the site of injury. Identifying better ways to promote wound healing is a major unmet need and biomedical materials with the ability to promote wound healing are urgently needed. Here, we report a thermosensitive black phosphorus hydrogel composed of black phosphorus nano-loaded drug silver sulfadiazine (SSD) and chitosan thermosensitive hydrogel for wound healing. The hydrogel has temperature-sensitive properties and enables the continuous release of SSD under near-infrared irradiation to achieve synergistic photothermal and antibacterial treatment. Additionally, it exerts antibacterial effects on Staphylococcus aureus. In a rat skin injury model, it promotes collagen deposition, boosts neovascularization, and suppresses inflammatory markers. In summary, the excellent thermosensitivity, biocompatibility, and wound-healing-promoting qualities of the reported thermosensitive hydrogel make it suitable as an ideal wound dressing in the clinic.
Topics: Animals; Rats; Silver Sulfadiazine; Hydrogels; Anti-Bacterial Agents; Wound Healing; Phosphorus
PubMed: 37715259
DOI: 10.1186/s12951-023-02054-3 -
Heliyon Jan 2024Antibiotics are widely used in intensive animal husbandry in the Netherlands and are subsequently emitted to soil via manure. To predict degradation and mobility in...
Antibiotics are widely used in intensive animal husbandry in the Netherlands and are subsequently emitted to soil via manure. To predict degradation and mobility in soil, generic sorption models have been derived. However, most of the coefficients used in generic models are based on a limited range of soils and have not been validated for agricultural soils in the Netherlands. To improve model predictions and assess to what extent differences among soils affect sorption and degradation, an experimental study has been performed. Using a recently developed experimental approach, both the degradation (DT50) and mobility () of eight selected commonly used antibiotics were determined in 29 typical Dutch agricultural soils. Median DT50 values range from 5.3 days for Sulfadiazine to 120 days for Trimethoprim but are affected by soil type. The ratio of the lowest and highest DT50 for a given antibiotic among soils can be as large as 151, for Tylosin. Measured values of the logK also range from 0.19 for Sulfadiazine to more than 2 for Doxycycline, Flumequine, Trimethoprim, Tylosin and Enrofloxacine. The impact of soil on is large, especially for more mobile antibiotics such as Sulfadoxine and Sulfadiazine. Both the range in DT50 and can be predicted reasonably well using a Freundlich type regression model that accounts for the variation in soil type and sampling depth. Organic matter, iron oxides, pH and clay content appear to be the main constituents and explain between 29 % (Trimethoprim) and 77 % of the variation in DT50 and between 64 % (Lincomycin) and 87 % (Sulfadoxine and Sulfadiazine) of the variation of . The effect of depth on DT50 and is however limited. The information thus obtained in combination with local data on soil type can be used to more accurately predict the potential risk of relevant antibiotics in soil and transport to ground- and nearby surface waters.
PubMed: 38187236
DOI: 10.1016/j.heliyon.2023.e23718 -
Veterinary World Dec 2023Potato ( L.) is mainly characterized by its antioxidant and healing properties. Therefore, this study aimed to evaluate the effects of an ointment based on L. "papa...
BACKGROUND AND AIM
Potato ( L.) is mainly characterized by its antioxidant and healing properties. Therefore, this study aimed to evaluate the effects of an ointment based on L. "papa tumbay" on burns induced in Balb/c mice ().
MATERIALS AND METHODS
The experimental animals were divided into four groups (n = 5/group) 48 h before second-degree burns were inducted. After epilating the loin areas of the mice and anesthetizing them with ketamine/xylazine (80 mg/kg/10 mg/kg) through intraperitoneal (i.p.) route, a round metal rod (0.7 cm in diameter) was placed on the depilated skin at a temperature of 100°C for 5 s. Group I was not given any treatment, Group II was treated with silver sulfadiazine (1%), and the other two groups (III and IV) were treated with the ointment formulated based on L. "papa tumbay" at 1% and 2%, respectively. After performing the treatment for 21 days, the mice were euthanized using i.p. sodium pentobarbital (185 mg/kg) to obtain skin samples. The samples were preserved in 10% neutral-buffered formalin and subjected to histopathological analysis.
RESULTS
We found statistically significant differences in the histopathological sections between the groups (p < 0.05). The abundant collagen and fibroblasts observed in the direction of the dermis in Groups III and IV indicate that the phytoconstituents present in the potato might promote the healing of the second-degree burns until day 21 of treatment.
CONCLUSION
Our findings showed that the ointments based on the ethanolic extracts of L. "papa tumbay," especially the 2% ointment, might accelerate the healing of second-degree burns induced in Balb/c mice.
PubMed: 38328356
DOI: 10.14202/vetworld.2023.2440-2445 -
Journal of Chromatographic Science May 2024A direct and precise isocratic RP-HPLC method for simultaneous determination of silver sulfadiazine (SSD) and sodium hyaluronate (SH) in the presence of methyl (MP) and...
Development and Validation of a RP-HPLC Method for the Simultaneous Determination of Silver Sulfadiazine and Sodium Hyaluronate in the Presence of Methyl and Propyl Paraben in a Pharmaceutical Cream for Burns.
A direct and precise isocratic RP-HPLC method for simultaneous determination of silver sulfadiazine (SSD) and sodium hyaluronate (SH) in the presence of methyl (MP) and propyl parabens (PP) was developed and validated. Agilent chromatograph with X-Select C18 column (250 × 4.6 mm2, 5 μm) was used. Chromatographic separation was achieved using a mobile phase consisting of acetonitrile and 0.05 M phosphate buffer (pH 5.0 to which added triethyl amine 0.5 ml/L), at a ratio 35: 65 v/v. Elution was used at flow rate of 1.0 mL/min at ambient temperature with UV detection at 205 nm. The retention times for SH, SSD, MP and PP were 1.49, 3.3, 6.7 and 19.5 min, respectively. The presented chromatographic method was fully validated in accordance with ICH requirements, it was valid over linearity ranges of (0.80-100.00 μg/mL) and (3.20-100.00 μg/mL) for SSD and SH, respectively. Acceptable precision and accuracy were obtained for concentrations over the standard curve ranges and the sensitivity of the method, as the limits of detection and quantification for each active ingredient was also determined. The validated method was successfully applied for the quantification of SSD and SH in pharmaceutical cream formulation and the mean recovery % ± SD were 100.93 ± 0.985 and 100.05 ± 0.668 for SSD and SH; respectively, indicating satisfactory accuracy of the method.
PubMed: 38745433
DOI: 10.1093/chromsci/bmae028 -
Burns : Journal of the International... Apr 2024Burns are a major global healthcare concern, often complicated by the presence of bacteria such as Pseudomonas aeruginosa in the wounds. Silver-based dressings are...
Burns are a major global healthcare concern, often complicated by the presence of bacteria such as Pseudomonas aeruginosa in the wounds. Silver-based dressings are commonly used in the treatment of burns but can cause skin irritation and delay healing time. Medical-grade honey (MGH) provides an interesting alternative. This study investigated the antimicrobial effects and possible cytotoxicity of L-Mesitran Soft (MGH-gel) and its individual components, Medihoney (Manuka), Flammazine (silver sulphadiazine), and silver nitrate (AgNO) in an ex vivo human burn wound model. Bacterial survival and wound healing parameters, including re-epithelialization and keratinocyte proliferation were assessed. L-Mesitran, Flammazine, and AgNO reduced P. aeruginosa numbers below detection levels. L-Mesitran Soft exhibited a significantly stronger antimicrobial effect compared to Medihoney. The individual components of L-Mesitran contributed significantly to its antibacterial efficacy, thus suggesting synergistic activities. Moreover, L-Mesitran, Flammazine, and AgNO slightly inhibited re-epithelialization while Medihoney treatment resulted in a complete lack of re-epithelialization and keratinocyte proliferation. Furthermore, clinical cases illustrated the effectiveness of MGH therapy in infected burns. Overall, L-Mesitran Soft had similar effects as silver-based products on bacterial load and epidermal regeneration, but outperformed Medihoney. Therefore, supplemented MGH could be used as an effective alternative to silver-based dressings for P. aeruginosa-infected burns.
Topics: Humans; Silver Sulfadiazine; Honey; Burns; Wound Healing; Anti-Bacterial Agents; Bacteria
PubMed: 37940425
DOI: 10.1016/j.burns.2023.10.009 -
Journal of Integrative Medicine Sep 2023This study tests the efficacy of Bletilla striata polysaccharide (BSP), carboxymethyl chitosan (CMC), baicalin (BA) and silver titanate (ST) in a wound dressings to...
OBJECTIVE
This study tests the efficacy of Bletilla striata polysaccharide (BSP), carboxymethyl chitosan (CMC), baicalin (BA) and silver titanate (ST) in a wound dressings to fight infection, promote healing and provide superior biocompatibility.
METHODS
The antibacterial activity of BA and ST was evaluated in vitro using the inhibition zone method. BA/ST/BSP/CMC porous sponge dressings were prepared and characterized. The biocompatibility of BA/ST/BSP/CMC was assessed using the cell counting kit-8 assay. The therapeutic effect of BA/ST/BSP/CMC was further investigated using the dorsal skin burn model in Sprague-Dawley rats.
RESULTS
The wound dressing had good antibacterial activity against Escherichia coli and Staphylococcus aureus through BA and ST, while the combination of BSP and CMC played an important role in promoting wound healing. The BA/ST/BSP/CMC porous sponge dressings were prepared using a freeze-drying method with the concentrations of BA and ST at 20 and 0.83 mg/mL, respectively, and the optimal ratio of 5% BSP to 4% CMC was 1:3. The average porosity, water absorption and air permeability of BA/ST/BSP/CMC porous sponge dressings were measured to be 90.43%, 746.1% and 66.60%, respectively. After treatment for 3 and 7 days, the healing rates of the BA/ST/BSP/CMC group and BA/BSP/CMC group were significantly higher than those of the normal saline (NS) group and silver sulfadiazine (SSD) group (P < 0.05). Interleukin-1β expression in the BA/ST/BSP/CMC group at 1 and 3 days was significantly lower than that in the other three groups (P < 0.05). After being treated for 3 days, vascular endothelial growth factor expression in the BA/BSP/CMC group and BA/ST/BSP/CMC group was significantly higher than that in the NS group and SSD group (P < 0.05). Inspection of histological sections showed that the BA/ST/BSP/CMC group and BA/BSP/CMC group began to develop scabbing and peeling of damaged skin after 3 days of treatment, indicating accelerated healing relative to the NS group and SSD group.
CONCLUSION
The optimized concentration of BA/ST/BSP/CMC dressing was as follows: 6 mg BSP, 14.4 mg CMC, 0.5 mg ST and 12 mg BA. The BA/ST/BSP/CMC dressing, containing antibacterial constituents, was non-cytotoxic and effective in accelerating the healing of burn wounds, making it a promising candidate for wound healing. Please cite this article as: Gong YR, Zhang C, Xiang X, Wang ZB, Wang YQ, Su YH, Zhang HQ. Baicalin, silver titanate, Bletilla striata polysaccharide and carboxymethyl chitosan in a porous sponge dressing for burn wound healing. J Integr Med. 2023; 21(5): 487-495.
Topics: Rats; Animals; Chitosan; Silver; Porosity; Vascular Endothelial Growth Factor A; Rats, Sprague-Dawley; Wound Healing; Polysaccharides; Bandages; Burns; Anti-Bacterial Agents; Silver Sulfadiazine
PubMed: 37544834
DOI: 10.1016/j.joim.2023.07.002 -
Chemosphere Dec 2023Surface electron transport and transfer of catalysts have important consequences for persulfate (PS) activation in PS system. In this paper, an electron-rich Cu-beta...
Surface electron transport and transfer of catalysts have important consequences for persulfate (PS) activation in PS system. In this paper, an electron-rich Cu-beta zeolites catalyst was synthesized utilizing a straightforward solid-state ion exchange technique to efficiently degrade sulfadiazine. The X-ray diffraction (XRD) and fourier transform infrared spectroscopy (FTIR) results revealed that Cu element substitutes Al element and enters the beta molecular sieve framework smoothly. Furthermore, the X-ray photoelectron spectroscopy (XPS) measurements demonstrated that the Cu-beta catalyst is primarily Cu. Cu-beta zeolites catalyst can exhibit excellent catalytic activity to degrade sulfadiazine with the oxidant of PS. The optimal sulfadiazine removal performance was explored by adjusting reaction parameters, including sulfadiazine concentration, catalyst dosage, oxidant dosage, and solution pH. The sulfadiazine removal efficiency in the Cu-beta zeolites/PS system could reach 90.5% at the optimal reaction condition ([PS] = 0.5 g/L, [Cu-beta zeolites] = 1.0 g/L, pH = 7.0) with 50 mg/L of sulfadiazine. Meanwhile, The degradation efficiency was less affected by anionic interference (Cl, SO, HCO). The surface electron transport and transfer of the Cu-beta zeolites catalyst were significant causes for the remarkable degradation performance. According to electron paramagnetic resonance (EPR) and quenching studies, the Cu-beta zeolites/PS system was mostly dominated by SO in the degradation of sulfadiazine. Furthermore, two possible pathways for sulfadiazine degradation were proposed according to the analysis of intermediate products detected by the liquid chromatography-mass spectrometry (LC-MS).
Topics: Sulfadiazine; Oxidation-Reduction; Zeolites; Electrons; Oxidants; Water Pollutants, Chemical
PubMed: 37777089
DOI: 10.1016/j.chemosphere.2023.140300