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Frontiers in Microbiology 2024Free-living amoebae are an extensive group of protistans that can be found in a wide variety of environments. Among them, the genus and stand out as two of the most...
INTRODUCTION
Free-living amoebae are an extensive group of protistans that can be found in a wide variety of environments. Among them, the genus and stand out as two of the most pathogenic amoebae and with a higher number of reported cases. is mainly found in warm freshwater water bodies whereas amoebae of the genus are broadly distributed through natural and anthropogenic environments. In this regard, the management and the control of the amoebic populations in swimming pools has become a major public health challenge for institutions.
METHODS
The aim of this work was to evaluate the growth pattern of trophozoites of and at different temperatures and salt concentrations.
RESULTS AND DISCUSSION
Our results showed that resisted a higher concentration of salt than . Moreover, no trophozoites could withstand the salt levels of the sea in conditions. This work supports the contention that salinity could represent an important and useful tool for the control of the most pathogenic amoebic populations in recreational water bodies.
PubMed: 38426057
DOI: 10.3389/fmicb.2024.1356452 -
Antioxidants (Basel, Switzerland) Dec 2023is a ubiquitous genus of amoebae that can act as opportunistic parasites in both humans and animals, causing a variety of ocular, nervous and dermal pathologies....
is a ubiquitous genus of amoebae that can act as opportunistic parasites in both humans and animals, causing a variety of ocular, nervous and dermal pathologies. Despite advances in therapy, the management of patients with infections remains a challenge for health services. Therefore, there is a need to search for new active substances against Acanthamoebae. In the present study, we evaluated the amoebicidal activity of nitroxoline against the trophozoite and cyst stages of six different strains of . The strain showed the lowest IC value in the trophozoite stage (0.69 ± 0.01 µM), while the strain L-10 showed the lowest IC value in the cyst stage (0.11 ± 0.03 µM). In addition, nitroxoline induced in treated trophozoites of features compatibles with apoptosis and autophagy pathways, including chromatin condensation, mitochondrial malfunction, oxidative stress, changes in cell permeability and the formation of autophagic vacuoles. Furthermore, proteomic analysis of the effect of nitroxoline on trophozoites revealed that this antibiotic induced the overexpression and the downregulation of proteins involved in the apoptotic process and in metabolic and biosynthesis pathways.
PubMed: 38136200
DOI: 10.3390/antiox12122081