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Mass Spectrometry Reviews 2024Mass spectrometry (MS) has become a central technique in cancer research. The ability to analyze various types of biomolecules in complex biological matrices makes it... (Review)
Review
Mass spectrometry (MS) has become a central technique in cancer research. The ability to analyze various types of biomolecules in complex biological matrices makes it well suited for understanding biochemical alterations associated with disease progression. Different biological samples, including serum, urine, saliva, and tissues have been successfully analyzed using mass spectrometry. In particular, spatial metabolomics using MS imaging (MSI) allows the direct visualization of metabolite distributions in tissues, thus enabling in-depth understanding of cancer-associated biochemical changes within specific structures. In recent years, MSI studies have been increasingly used to uncover metabolic reprogramming associated with cancer development, enabling the discovery of key biomarkers with potential for cancer diagnostics. In this review, we aim to cover the basic principles of MSI experiments for the nonspecialists, including fundamentals, the sample preparation process, the evolution of the mass spectrometry techniques used, and data analysis strategies. We also review MSI advances associated with cancer research in the last 5 years, including spatial lipidomics and glycomics, the adoption of three-dimensional and multimodal imaging MSI approaches, and the implementation of artificial intelligence/machine learning in MSI-based cancer studies. The adoption of MSI in clinical research and for single-cell metabolomics is also discussed. Spatially resolved studies on other small molecule metabolites such as amino acids, polyamines, and nucleotides/nucleosides will not be discussed in the context.
Topics: Humans; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Artificial Intelligence; Metabolomics; Neoplasms; Lipidomics
PubMed: 36065601
DOI: 10.1002/mas.21804 -
Pharmaceuticals (Basel, Switzerland) Aug 2023Xerostomia, commonly known as dry mouth, is a widespread oral health malfunction characterized by decreased salivary flow. This condition results in discomfort, impaired... (Review)
Review
Xerostomia, commonly known as dry mouth, is a widespread oral health malfunction characterized by decreased salivary flow. This condition results in discomfort, impaired speech and mastication, dysphagia, heightened susceptibility to oral infections, and ultimately, a diminished oral health-related quality of life. The etiology of xerostomia is multifaceted, with primary causes encompassing the use of xerostomic medications, radiation therapy to the head and neck, and systemic diseases such as Sjögren's syndrome. Consequently, there is a growing interest in devising management strategies to address this oral health issue, which presents significant challenges due to the intricate nature of saliva. Historically, natural products have served medicinal purposes, and in contemporary pharmaceutical research and development, they continue to play a crucial role, including the treatment of xerostomia. In this context, the present review aims to provide an overview of the current state of knowledge regarding natural compounds and extracts for xerostomia treatment, paving the way for developing novel therapeutic strategies for this common oral health issue.
PubMed: 37631049
DOI: 10.3390/ph16081136 -
Journal of Gynecology Obstetrics and... Nov 2023The French National College of Obstetricians and Gynecologists (CNGOF) published guidelines for managing endometriosis-associated pain in 2018. Given the development of...
The French National College of Obstetricians and Gynecologists (CNGOF) published guidelines for managing endometriosis-associated pain in 2018. Given the development of new pharmacological therapies and a review that was published in 2021, most national and international guidelines now suggest a new therapeutic approach. In addition, a novel validated screening method based on patient questionnaires and analysis of 109-miRNA saliva signatures, which combines biomarkers and artificial intelligence, opens up new avenues for overcoming diagnostic challenges in patients with pelvic pain and for avoiding laparoscopic surgery when sonography and MRI are not conclusive. Dienogest (DNG) 2 mg has been a reimbursable healthcare expense in France since 2020, and, according to recent studies, it is at least as effective as combined hormonal contraception (CHC) and can be used as an alternative to CHC for first-line treatment of endometriosis-associated pain. Since 2018, the literature concerning the use of DNG has grown considerably, and the French guidelines should be modified accordingly. The levonorgestrel intrauterine system (LNG IUS) and other available progestins per os, including DNG, or the subcutaneous implant, can be offered as first-line therapy, gonadotropin-releasing hormone (GnRH) agonists with add-back therapy (ABT) as second-line therapy. Oral GnRH antagonists are promising new medical treatments for women with endometriosis-associated pain. They competitively bind to GnRH receptors in the anterior pituitary, preventing native GnRH from binding to GnRH receptors and from stimulating the secretion of luteinizing hormone and follicle-stimulating hormone. Consequently, estradiol and progesterone production is reduced. Oral GnRH antagonists will soon be on the market in France. Given their mode of action, their efficacy is comparable to that of GnRH agonists, with the advantage of oral administration and rapid action with no flare-up effect. Combination therapy with ABT is likely to allow long-term treatment with minimal impact on bone mass. GnRH antagonists with ABT may thus be offered as second-line treatment as an alternative to GnRH agonists with ABT. This article presents an update on the management of endometriosis-associated pain in women who do not have an immediate desire for pregnancy.
Topics: Female; Humans; Endometriosis; Receptors, LHRH; Artificial Intelligence; Pelvic Pain; Gonadotropin-Releasing Hormone; Hormone Antagonists
PubMed: 37669732
DOI: 10.1016/j.jogoh.2023.102664 -
European Journal of Translational... Jul 2023The purpose of this study was to investigate the effect of different solutions on the optical behavior of two types of 3D-printed resin (Detax and Asiga). The samples...
The purpose of this study was to investigate the effect of different solutions on the optical behavior of two types of 3D-printed resin (Detax and Asiga). The samples were randomly divided into 4 groups of cola, orange juice, tea and artificial saliva (control group) in the form of 14 x 10 mm blocks with a thickness of 1.5 ± 0.3 mm (10 samples in each group). By spectrophotometric device, the color of the examined samples was recorded in the CIE system and in three parameters *L (brightness), a* (red-green) and b* (blue-yellow) as the initial record. The color difference of the samples was recorded on 7 and 30 days after immersion as a secondary record. After that, using the formula, the color difference (ΔE) were calculated. T-test also was used to analyze the data. There was no significant difference between the amount of ΔE on 7th and 30th days after exposure to artificial saliva and cola and on 7th days after exposure to orange juice and tea. But on the 30th day, a significant difference was found in orange juice and tea drinks (p<0.05), so that the value of ΔE in Asiga printer was lower than Detax. ΔE changes in orange juice solution were more than other drinks. On the 30th day of immersion in tea drinks and orange juice, Asiga printer had significantly less color difference than Detax printer.
PubMed: 37767891
DOI: 10.4081/ejtm.2023.11493 -
ACS Central Science Jul 2023
PubMed: 37521799
DOI: 10.1021/acscentsci.3c00803 -
Dental Materials : Official Publication... Jul 2024Three-dimensional (3D) printing is increasingly used to fabricate dental restorations due to its enhanced precision, consistency and time and cost-saving advantages. The...
BACKGROUND
Three-dimensional (3D) printing is increasingly used to fabricate dental restorations due to its enhanced precision, consistency and time and cost-saving advantages. The properties of 3D-printed resin materials can be influenced by the chosen printing orientation which can impact the mechanical characteristics of the final products.
PURPOSE
The objective of this study was to evaluate the influence of printing orientation and artificial ageing on the Martens hardness (HM) and indentation modulus (E) of 3D-printed definitive and temporary dental restorative resins.
METHODS
Disk specimens (20 mm diameter × 2 mm height) were additively manufactured in three printing orientations (0°, 45°, 90°) using five 3D-printable resins: VarseoSmile Crownplus (VCP), Crowntec (CT), Nextdent C&B MFH (ND), Dima C&B temp (DT), and GC temp print (GC). The specimens were printed using a DLP 3D-printer (ASIGA MAX UV), while LavaTM Ultimate (LU) and Telio CAD (TC) served as milled control materials. Martens hardness (HM) and indentation modulus (E) were tested both before and after storage in distilled water and artificial saliva for 1, 30, and 90 days at 37 °C.
RESULTS
90° printed specimens exhibited higher HM than the other orientations at certain time points, but no significant differences were observed in HM and E between orientations for all 3D-printed materials after 90 days of ageing in both aging media. LU milled control material exhibited the highest HM and E among the tested materials, while TC, the other milled control, showed similar values to the 3D printed resins. CT and VCP (definitive resins) and ND displayed higher Martens parameters compared to DT and GC (temporary resins). The hardness of the 3D-printed materials was significantly impacted by artificial ageing compared to the controls, with ND having the least hardness reduction percentage amongst all 3D-printed materials. The hardness reduction percentage in distilled water and artificial saliva was similar for all materials except for TC, where higher reduction was noted in artificial saliva.
SIGNIFICANCE
The used 3D printed resins cannot yet be considered viable alternatives to milled materials intended for definitive restorations but are preferable for use as temporary restorations.
Topics: Printing, Three-Dimensional; Hardness; Materials Testing; Elastic Modulus; Dental Materials; Surface Properties; Composite Resins; Time Factors; Dental Restoration, Permanent; Resins, Synthetic
PubMed: 38735775
DOI: 10.1016/j.dental.2024.05.005 -
European Journal of Obstetrics,... Dec 2023In contrast to miRNA expression, little attention has been given to piwiRNA (piRNA) expression among endometriosis patients. The aim of the present study was to explore...
OBJECTIVES
In contrast to miRNA expression, little attention has been given to piwiRNA (piRNA) expression among endometriosis patients. The aim of the present study was to explore the human piRNAome and to investigate a potential piRNA saliva-based diagnostic signature for endometriosis.
METHODS
Data from the prospective "ENDOmiRNA" study (ClinicalTrials.gov Identifier: NCT04728152) were used. Saliva samples from 200 patients were analyzed in order to evaluate human piRNA expression using the piRNA bank. Next Generation Sequencing (NGS), barcoding of unique molecular identifiers and both Artificial Intelligence (AI) and machine learning (ML) were used. For each piRNA, sensitivity, specificity, and ROC AUC values were calculated for the diagnosis of endometriosis.
RESULTS
201 piRNAs were identified, none had an AUC ≥ 0.70, and only three piRNAs (piR-004153, piR001918, piR-020401) had an AUC between ≥ 0.6 and < 0.70. Seven were differentially expressed: piR-004153, piR-001918, piR-020401, piR-012864, piR-017716, piR-020326 and piR-016904. The respective correlation and accuracy to diagnose endometriosis according to the F1-score, sensitivity, specificity, and AUC ranged from 0 to 0.862 %, 0-0.961 %, 0.085-1, and 0.425-0.618. A correlation was observed between the patients' age (≥35 years) and piR-004153 (p = 0.002) and piR-017716 (p = 0.030). Among the 201 piRNAs, four were differentially expressed in patients with and without hormonal treatment: piR-004153 (p = 0.015), piR-020401 (p = 0.001), piR-012864 (p = 0.036) and piR-017716 (p = 0.009).
CONCLUSION
Our results support the link between piRNAs and endometriosis physiopathology and establish its utility as a potential diagnostic biomarker using saliva samples. Per se, piRNA expression should be analyzed along with the clinical status of a patient.
Topics: Female; Humans; Adult; Piwi-Interacting RNA; RNA, Small Interfering; Endometriosis; Artificial Intelligence; Prospective Studies; Biomarkers
PubMed: 37857147
DOI: 10.1016/j.ejogrb.2023.10.015 -
Nature Communications Nov 2023Guided bone regeneration aided by the application of occlusive membranes is a promising therapy for diverse inflammatory periodontal diseases. Symbiosis, homeostasis...
Guided bone regeneration aided by the application of occlusive membranes is a promising therapy for diverse inflammatory periodontal diseases. Symbiosis, homeostasis between the host microbiome and cells, occurs in the oral environment under normal, but not pathologic, conditions. Here, we develop a symbiotically integrating occlusive membrane by mimicking the tooth enamel growth or multiple nucleation biomineralization processes. We perform human saliva and in vivo canine experiments to confirm that the symbiotically integrating occlusive membrane induces a symbiotic healing environment. Moreover, we show that the membrane exhibits tractability and enzymatic stability, maintaining the healing space during the entire guided bone regeneration therapy period. We apply the symbiotically integrating occlusive membrane to treat inflammatory-challenged cases in vivo, namely, the open and closed healing of canine premolars with severe periodontitis. We find that the membrane promotes symbiosis, prevents negative inflammatory responses, and improves cellular integration. Finally, we show that guided bone regeneration therapy with the symbiotically integrating occlusive membrane achieves fast healing of gingival soft tissue and alveolar bone.
Topics: Humans; Alveolar Bone Loss; Guided Tissue Regeneration, Periodontal; Wound Healing; Periodontitis; Gingiva; Membranes, Artificial; Bone Regeneration
PubMed: 38001080
DOI: 10.1038/s41467-023-43428-3 -
Materials (Basel, Switzerland) Oct 2023The pitting corrosion of orthodontic apparatus elements in the oral environment is an interest of both clinicians and scientists dealing with the assessment of the...
The pitting corrosion of orthodontic apparatus elements in the oral environment is an interest of both clinicians and scientists dealing with the assessment of the biocompatibility of medical materials. This work presents a study on the effect of ready-to-use Listerine and Meridol mouthwashes and sodium fluoride on the resistance of the commercial Remanium-type orthodontic archwire to pitting corrosion in artificial saliva at 37 °C. XRD, SEM, EDS, mechanical properties, and microhardness measurements were used to characterize the archwire. The in vitro corrosion resistance of the archwire was examined using the open-circuit potential method, electrochemical impedance spectroscopy, and anodic polarization curves. The physicochemical characteristics confirmed the presence of a bi-phase alloy with a mixed austenite/ferrite structure containing Fe 74.4(7) at.%, Cr 18.4(4) at.%, and Ni 7.2(4) at.%. The Fe-Cr-Ni alloy was characterized by high tensile strength and Vickers microhardness. EIS revealed the capacitive behavior with high corrosion resistance. It was found that the kinetics of pitting corrosion in the artificial saliva decreased in the presence of NaF and mouthwashes. The potentiodynamic characteristics confirmed the decrease in susceptibility to pitting corrosion after the modification of artificial saliva. The pitting corrosion mechanism of the self-passive oxide layer on the surface of the Fe-Cr-Ni electrode in the biological environment containing chloride ions was discussed in detail. Mechanical properties after corrosion tests were weakened.
PubMed: 37895772
DOI: 10.3390/ma16206791