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Microbiological Research Mar 2024The bark and leaves of the Eucommia ulmoides Oliv. (E. ulmoides) have good medicinal value. Studies show endophytes play important roles in host medicinal plant...
The bark and leaves of the Eucommia ulmoides Oliv. (E. ulmoides) have good medicinal value. Studies show endophytes play important roles in host medicinal plant secondary metabolite synthesis, with season being a key influencing factor. Therefore, we used 16 S rRNA to detect endophytic bacteria (EB) in E. ulmoides bark and leaves collected in winter, spring, summer, and autumn, and analyzed the contents of major active components respectively. The results showed that the species diversity and richness of EB of the E. ulmoides bark were higher than those of leaves in all seasons except fall. Among them, the higher species diversity and richness were found in the E. ulmoides bark in winter and spring. EB community structure differed significantly between medicinal tissues and seasons. Concurrently, the bark and leaves of E. ulmoides showed abundant characteristic EB across seasons. For active components, geniposidic acid showed a significant positive correlation with EB diversity and richness, while the opposite was true for aucubin. Additionally, some dominant EB exhibited close correlations with the accumulation of active components. Delftia, enriched in autumn, correlated significantly positively with aucubin. Notably, the impact of the same EB genera on active components differed across medicinal tissues. For example, Sphingomonas, enriched in summer, correlated significantly positively with pinoresinol diglucoside (PDG) in the bark, but with aucubin in the leaves. In summary, EB of E. ulmoides was demonstrated high seasonal dynamics and tissue specificity, with seasonal characteristic EB like Delftia and Sphingomonas correlating with the accumulation of active components in medicinal tissues.
Topics: Plants, Medicinal; Seasons; Eucommiaceae; Plant Bark; Plant Leaves; Bacteria; Iridoid Glucosides
PubMed: 38103467
DOI: 10.1016/j.micres.2023.127567 -
Insects Feb 2024bacteria (phylum Proteobacteria) are ubiquitous intracellular parasites of diverse invertebrates. In insects, coevolution has forged mutualistic associations with...
bacteria (phylum Proteobacteria) are ubiquitous intracellular parasites of diverse invertebrates. In insects, coevolution has forged mutualistic associations with species, influencing reproduction, immunity, development, pathogen resistance, and overall fitness. However, the impact of on other microbial associates within the insect microbiome, which are crucial for host fitness, remains less explored. The diamondback moth (), a major pest of cruciferous vegetables worldwide, harbors the dominant strain , known to distort its sex ratio. This study investigated the bacterial community diversity and dynamics across different developmental life stages and infection states in using high-throughput 16S rDNA amplicon sequencing. Proteobacteria and Firmicutes dominated the microbiome regardless of life stage or infection. However, the relative abundance of dominant genera, including an unclassified genus of Enterobacteriaceae, , , and , displayed significant stage-specific variations. While significant differences in bacterial diversity and composition were observed across life stages, infection had no substantial impact on overall diversity. Nonetheless, relative abundances of specific genera differed between infection states. Notably, exhibited a stable, high relative abundance across all stages and negatively correlated with an unclassified genus of Enterobacteriaceae, , and . Our findings provide a foundational understanding of the complex interplay between the host, , and the associated microbiome in , paving the way for a deeper understanding of their complex interactions and potential implications for pest control strategies.
PubMed: 38392551
DOI: 10.3390/insects15020132 -
Microbiology Resource Announcements Jun 2024a Gram-negative rod-shaped aerobic bacterium with environmental remediation functions. strain HA60 was isolated from a commercial nano-particle product,...
a Gram-negative rod-shaped aerobic bacterium with environmental remediation functions. strain HA60 was isolated from a commercial nano-particle product, nano-hydroxyapatite. We report that the genome of strain HA60 has a circular genome of 6,922,195 base pairs with a G+C content of 66.45%.
PubMed: 38700346
DOI: 10.1128/mra.00171-24 -
Pathogens (Basel, Switzerland) Nov 2023Styles transmit pollen-derived sperm nuclei from pollen to ovules, but also transmit environmental pathogens. The microbiomes of styles are likely important for...
Styles transmit pollen-derived sperm nuclei from pollen to ovules, but also transmit environmental pathogens. The microbiomes of styles are likely important for reproduction/disease, yet few studies exist. Whether style microbiome compositions are spatially responsive to pathogens is unknown. The maize pathogen enters developing grain through the style (silk). We hypothesized that treatment shifts the cultured transmitting silk microbiome (TSM) compared to healthy silks in a distance-dependent manner. Another objective of the study was to culture microbes for future application. Bacteria were cultured from husk-covered silks of 14 -treated diverse maize genotypes, proximal (tip) and distal (base) to the inoculation site. Long-read 16S sequences from 398 isolates spanned 35 genera, 71 species, and 238 OTUs. More bacteria were cultured from -inoculated tips (271 isolates) versus base (127 isolates); healthy silks were balanced. caused a collapse in diversity of ~20-25% across multiple taxonomic levels. Some species were cultured exclusively or, more often, from -treated silks (e.g., , , , , ). Overall, the results suggest that alters the TSM in a distance-dependent manner. Many isolates matched taxa that were previously identified using V4-MiSeq (core and -induced), but long-read sequencing clarified the taxonomy and uncovered greater diversity than was initially predicted (e.g., within ). These isolates represent the first comprehensive cultured collection from pathogen-treated maize silks to facilitate biocontrol efforts and microbial marker-assisted breeding.
PubMed: 38003787
DOI: 10.3390/pathogens12111322 -
Microbiology Resource Announcements Jan 2024Soil is a source for diverse microbes that possess useful biotechnological capabilities. Here, we report the genome sequences of seven bacterial isolates from the...
Soil is a source for diverse microbes that possess useful biotechnological capabilities. Here, we report the genome sequences of seven bacterial isolates from the species , , , , sp., and (two isolates) cultured from Dallas/Fort Worth metroplex soil samples.
PubMed: 38038466
DOI: 10.1128/MRA.00357-23 -
MBio Jan 2024Society uses thousands of organofluorine compounds, sometimes denoted per- and polyfluoroalkyl substances (PFAS), in hundreds of products, but recent studies have shown...
Society uses thousands of organofluorine compounds, sometimes denoted per- and polyfluoroalkyl substances (PFAS), in hundreds of products, but recent studies have shown some to manifest human and environmental health effects. As a class, they are recalcitrant to biodegradation, partly due to the paucity of fluorinated natural products to which microbes have been exposed. Another limit to PFAS biodegradation is the intracellular toxicity of fluoride anion generated from C-F bond cleavage. The present study identified a broader substrate specificity in an enzyme originally studied for its activity on the natural product fluoroacetate. A recombinant expressing this enzyme was used here as a model system to better understand the limits and effects of a high level of intracellular fluoride generation. A fluoride stress response has evolved in bacteria and has been described in spp. The present study is highly relevant to organofluorine compound degradation or engineered biosynthesis in which fluoride anion is a substrate.
Topics: Humans; Fluorides; Pseudomonas; Fluoroacetates; Biodegradation, Environmental; Fluorocarbons
PubMed: 38063407
DOI: 10.1128/mbio.02785-23