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Clinical Biochemistry Mar 2024An analytical benchmark for high-sensitivity cardiac troponin (hs-cTn) assays is to achieve a coefficient of variation (CV) of ≤ 10.0 % at the 99th percentile upper...
BACKGROUND
An analytical benchmark for high-sensitivity cardiac troponin (hs-cTn) assays is to achieve a coefficient of variation (CV) of ≤ 10.0 % at the 99th percentile upper reference limit (URL) used for the diagnosis of myocardial infarction. Few prospective multicenter studies have evaluated assay imprecision and none have determined precision at the female URL which is lower than the male URL for all cardiac troponin assays.
METHODS
Human serum and plasma matrix samples were constructed to yield hs-cTn concentrations near the female URLs for the Abbott, Beckman, Roche, and Siemens hs-cTn assays. These materials were sent (on dry ice) to 35 Canadian hospital laboratories (n = 64 instruments evaluated) participating in a larger clinical trial, with instructions for storage, handling, and monthly testing over one year. The mean concentration, standard deviation, and CV for each instrument type and an overall pooled CV for each manufacturer were calculated.
RESULTS
The CVs for all individual instruments and overall were ≤ 10.0 % for two manufacturers (Abbott CV = 6.3 % and Beckman CV = 7.0 %). One of four Siemens Atellica instruments yielded a CV > 10.0 % (CV = 7.7 %), whereas 15 of 41 Roche instruments yielded CVs > 10.0 % at the female URL of 9 ng/L used worldwide (6 cobas e411, 1 cobas e601, 4 cobas e602, and 4 cobas e801) (CV = 11.7 %). Four Roche instruments also yielded CVs > 10.0 % near the female URL of 14 ng/L used in the United States (CV = 8.5 %).
CONCLUSIONS
The number of instruments achieving a CV ≤ 10.0 % at the female 99th-percentile URL varies by manufacturer and by instrument. Monitoring assay precision at the female URL is necessary for some assays to ensure optimal use of this threshold in clinical practice.
Topics: Humans; Male; Female; Prospective Studies; Canada; Myocardial Infarction; Biological Assay; Troponin; Troponin T; Biomarkers; Reference Values
PubMed: 38360198
DOI: 10.1016/j.clinbiochem.2024.110731 -
Acta Tropica Jun 2024Mosquito-borne diseases constitute a significant global impact on public and animal health. Climatic variables are recognized as major drivers in the mosquitoes' life...
Mosquito-borne diseases constitute a significant global impact on public and animal health. Climatic variables are recognized as major drivers in the mosquitoes' life history, principally rainfall and temperature, which directly influence mosquito abundance. Likewise, urbanization changes environmental conditions, and understanding how environmental variables and urbanization influence mosquito dynamics is crucial for the integrated management of mosquito-borne diseases, especially in the context of climate change. In this study, our aim was to observe the effect of temperature, rainfall, and the percentage of impervious surface on the abundance of mosquito species over a temporal scale of one complete year of fortnightly samplings, spanning from June 2021 to June 2022 in Yucatan, Mexico. We selected nine localities along an urbanization gradient (three natural, three rural, and three urban) from Mérida City to Reserva de la Biosfera Ría Celestún. Using BG-traps, mosquitoes were collected biweekly at each locality. Additionally, we estimated the percentage of impervious surface. Daily data of the maximum, mean and minimum temperatures, diurnal temperature range and rainfall were accumulated weekly. We calculated the accumulated quantities of temperatures and rainfall and lagged from one to four weeks before sampling for each locality. Generalized linear mixed models were then performed to study the influence of environmental variables and percentage of impervious surfaces on each of the 15 most abundant species. A total of 131,525 mosquitoes belonging to 11 genera and 49 species were sampled with BG-Sentinel traps baited with BG-lure and dry ice. The most frequently significative variable is the accumulated precipitation four weeks before the sampling. We observed a positive relationship between Cx. quinquefasciatus and Cx. thriambus with the diurnal temperature range. For Ae. aegypti, we observed a positive relationship with minimum temperature. Conversely, the percentage of impervious surface serves as a proxy of anthropogenic influence and helped us to distinguishing species exhibiting habitat preference for urban and rural environments, versus those preferring natural habitats. Our results characterize the species-specific effects of environmental variables (temperature, rainfall and impervious surface) on mosquito abundance.
PubMed: 38851624
DOI: 10.1016/j.actatropica.2024.107275 -
Scientific Reports May 2024A volatile sessile liquid droplet or a sublimating solid manifests levitation on its own vapor when placed on a sufficiently heated surface, illustrating the Leidenfrost...
A volatile sessile liquid droplet or a sublimating solid manifests levitation on its own vapor when placed on a sufficiently heated surface, illustrating the Leidenfrost phenomenon. In this study, we introduce a non-invasive capacitance method for investigating this phenomenon, offering a potentially simpler alternative to existing optical techniques. The designed sensor features in-plane miniaturized electrodes forming a double-comb structure, also known as an interdigitated capacitor. Initially, the sensor's capacitance is characterized for various distances between the sensor and a dielectric material. The influence of the sensor substrate material and the spacing between the electrodes on the sensor's capacitance is also investigated. To demonstrate the feasibility of the method, a sublimating dry ice pellet is placed on the capacitive sensor, and its performance is evaluated. We present results for the dimensionless vapor layer thickness and the pellet's lifetime at different substrate temperatures, derived from the capacitance output. The results are compared with Optical Coherence Tomography (OCT) data, serving as a benchmark. While the temporal evolution of the sensor's output, variation in the dimensionless vapor layer thickness, and the lifetime of the dry ice pellet align with expected results from OCT, notable quantitative deviations are observed. These deviations are attributed to practical experimental limitations rather than shortcoming in the sensor's working principle. Although this necessitates further investigation, the methodology presented in this paper can potentially serve as an alternative for the detection and measurement of Leidenfrost vapor layers.
PubMed: 38719905
DOI: 10.1038/s41598-024-61222-z -
International Journal of Molecular... May 2024In the last few years, several studies have emphasized the existence of injury-specific EV "barcodes" that could have significant importance for the precise diagnosis of...
In the last few years, several studies have emphasized the existence of injury-specific EV "barcodes" that could have significant importance for the precise diagnosis of different organ injuries in polytrauma patients. To expand the research potential of the NTF (network trauma research) biobank of polytraumatized patients, the NTF research group decided to further establish a biobank for EVs. However, until now, the protocols for the isolation, characterization, and storage of EVs for biobank purposes have not been conceptualized. Plasma and serum samples from healthy volunteers (n = 10) were used. Three EV isolation methods of high relevance for the work with patients' samples (ultracentrifugation, size exclusion chromatography, and immune magnetic bead-based isolation) were compared. EVs were quantified using nanoparticle tracking analysis, EV proteins, and miRNAs. The effects of different isolation solutions; the long storage of samples (up to 3 years); and the sensibility of EVs to serial freezing-thawing cycles and different storage conditions (RT, 4/-20/-80 °C, dry ice) were evaluated. The SEC isolation method was considered the most suitable for EV biobanking. We did not find any difference in the quantity of EVs between serum and plasma-EVs. The importance of particle-free PBS as an isolation solution was confirmed. Plasma that has been frozen for a long time can also be used as a source of EVs. Serial freezing-thawing cycles were found to affect the mean size of EVs but not their amount. The storage of EV samples for 5 days on dry ice significantly reduced the EV protein concentration.
Topics: Humans; Extracellular Vesicles; Biological Specimen Banks; Multiple Trauma; Specimen Handling; Chromatography, Gel; Male; Ultracentrifugation; MicroRNAs; Adult; Female
PubMed: 38891833
DOI: 10.3390/ijms25115645 -
Frontiers in Veterinary Science 2023Information on slaughter procedures for farmed fish in aquaculture is limited, both in Europe and in Italy, due to a general lack of field data. The aim of this study...
Information on slaughter procedures for farmed fish in aquaculture is limited, both in Europe and in Italy, due to a general lack of field data. The aim of this study was to gather information on the procedures used to slaughter fish in Italy and to discuss them considering the WOAH and EFSA recommendations on fish welfare. Using a questionnaire survey, data were collected by official veterinarians in 64 slaughtering facilities where 20 different species of fish were slaughtered. The main species slaughtered were rainbow trout (; 29/64), followed by European sea bass (; 21/64), sea bream (; 21/64), Arctic char (; 14/64), European eel (; 11/64), sturgeon ( spp; 11/64), common carp (; 6/64), and brown trout ( L.; 5/64). The most applied stunning/killing methods were "asphyxia in ice/thermal shock" and "electric in water bath," followed by "percussion," "asphyxia in air," and "electric dry system." After the application of the method, the assessment of the fish level of unconsciousness was practiced in 72% of the facilities using more than one indicator, with "breathing" and "coordinated movements" the most practiced. The collected data showed a discrepancy between the available recommendations about the welfare of fish at slaughter and what is practiced in many production sites, but for many species precise recommendations are still not available.
PubMed: 37869496
DOI: 10.3389/fvets.2023.1253151 -
Optics Express Jan 2024Atmospheric transport processes and conditions can cause primary aerosols to interact, giving rise to secondary aerosols with unique chemical and physical properties....
Atmospheric transport processes and conditions can cause primary aerosols to interact, giving rise to secondary aerosols with unique chemical and physical properties. These new species of aerosols can potentially influence the light-scattering properties of the aerosol ensemble and thus the climate system in ways that are not yet fully understood. In this study, the effects of different aerosol types on the scattering of incident solar radiation are modeled and the contribution of secondary aerosols to the aerosol scattering ensemble is highlighted. Using the discrete dipole approximation method, the scattering properties of freshwater droplets, sea salts (liquid, dry, and wet solids), ice crystals, clay minerals, clay particles coated with a thin film of water and sea salt droplets, black carbon (BC), and a complex particle of clay, sea salt, and BC with sulphate coating are calculated and compared. The calculations assume a spherical particle shape model for marine aerosols, a distorted cube for wet salts and ice, and a distorted ellipse with an induced surface roughness length for terrestrial aerosols at a size parameter of x=5 and a wavelength range of 400 to 750 nm. The results show that tiny ice crystals trapped in freshwater droplets are the most efficient atmospheric scatterers, followed by sea salt droplets, while BC absorbs the most compared to other aerosols studied. On average, the atmospheric interaction between marine and terrestrial aerosols is able to enhance atmospheric light scattering and polarisation by aerosols compared to terrestrial aerosols. This study suggests that the scenario in which there are many freshwater aerosols in the atmosphere can be very healthy for the Earth's system compared to other aerosols. Therefore, we suggest that when formulating the radiative properties of aerosols in climate models, the scenarios of dominant freshwater aerosols and the contribution of secondary aerosols should not be ignored. The results presented here may be useful in the fields of Geoengineering and Aerosol-cloud microphysics.
PubMed: 38297658
DOI: 10.1364/OE.514164 -
Nature Communications Apr 2024All-solid-state batteries using Si as the anode have shown promising performance without continual solid-electrolyte interface (SEI) growth. However, the first cycle...
All-solid-state batteries using Si as the anode have shown promising performance without continual solid-electrolyte interface (SEI) growth. However, the first cycle irreversible capacity loss yields low initial Coulombic efficiency (ICE) of Si, limiting the energy density. To address this, we adopt a prelithiation strategy to increase ICE and conductivity of all-solid-state Si cells. A significant increase in ICE is observed for LiSi anode paired with a lithium cobalt oxide (LCO) cathode. Additionally, a comparison with lithium nickel manganese cobalt oxide (NCM) reveals that performance improvements with Si prelithiation is only applicable for full cells dominated by high anode irreversibility. With this prelithiation strategy, 15% improvement in capacity retention is achieved after 1000 cycles compared to a pure Si. With LiSi, a high areal capacity of up to 10 mAh cm is attained using a dry-processed LCO cathode film, suggesting that the prelithiation method may be suitable for high-loading next-generation all-solid-state batteries.
PubMed: 38582753
DOI: 10.1038/s41467-024-47352-y -
Saudi Journal of Biological Sciences Jan 2024L. is an annual halophyte that originated from southern Africa. However, little has been reported about factors that modulate the quantity of secondary metabolites in...
L. is an annual halophyte that originated from southern Africa. However, little has been reported about factors that modulate the quantity of secondary metabolites in the plant. In this study, the twin effect of different nitrogen concentrations (0.36, 0.6, 0.8 g/L) and growing media (LECA clay, peat, vermiculite and silica sand) on plant growth, chlorophyll contents, minerals, proximate and antioxidant metabolites in hydroponically cultivated was investigated. This is important to determine the dosage of N fertilizer that will optimize the bio-productivity and biosynthesis of secondary metabolites and antioxidants in grown in a hydroponic system. The untreated plant (0 g/L N) was taken as the control. At the end of the experiment, optimum yields in leaf number (9.2), fresh weight (50.40 g), Ca, N, and Protein (34.04 %) were recorded in grown with peat enhanced with different dosages of N-fertilizer. Likewise, chlorophyll level, dry weight, ABTS/TEAC, FRAP, ADF and NDF contents were optimized in LECA clay treated with N-fertilizer. Silica sand with 0.36 g/L dosage of nitrogen fertilizer optimized P, Mn and Zn levels, so also the moisture (9.83 % at 0.8 g/L N), fat (2.38 %, 0 g/L N) and carbohydrates (44.98 and 44.95 %). The highest ash content, Mg and Fe were recorded in the untreated vermiculite as well as polyphenols and K, at 0.6 g/L; Cu and root length (14.60 cm), at 0.8 g/L. In conclusion, different dosages of nitrogen fertilizer and growing media could enhance the growth potential, chlorophyll, phytochemicals, and nutritional properties of .
PubMed: 38161387
DOI: 10.1016/j.sjbs.2023.103876 -
PLoS Neglected Tropical Diseases Jan 2024The central component of mosquito and vector surveillance programs globally is the adult mosquito trap, which is intended to collect host-seeking mosquitoes. The...
The central component of mosquito and vector surveillance programs globally is the adult mosquito trap, which is intended to collect host-seeking mosquitoes. The miniature CDC trap is a widely distributed trap style in part due to its relative affordability and compact nature. Despite already being a simple trap, in-house production methods, such as 3D printing, could improve the accessibility of the CDC trap by eliminating some of the supply chain variables. We present here several trials with the Salt Lake City (SLC) trap, a three-dimensional (3D) printed trap design. Functional assessments were made on secondary components and found no statistically significant differences when comparing CO2 line height (above vs. below fan), battery types (sealed lead acid vs. USB battery pack), and trap body collection shape (funnel body vs. simple/straight body). The SLC trap was compared directly to a commercial equivalent, the ABC trap, with comparative assessment on species diversity and evenness in collections and found to be statistically equivalent on all metrics. Methods also detail an accompanying optional transport system for a pressurized CO2/regulator set-up, should a practitioner elect not to use dry ice. Our final design is presented here with the publicly published stereolithography (STL) files and a detailed outline of the transport container system. Alternative models are available for in-house manufacture of mosquito traps, and we contribute these designs in an effort to stimulate further growth in vector surveillance.
Topics: Animals; United States; Culicidae; Carbon Dioxide; Mosquito Vectors; Centers for Disease Control and Prevention, U.S.; Mosquito Control
PubMed: 38198453
DOI: 10.1371/journal.pntd.0011899 -
The Science of the Total Environment Aug 2024Due to extreme weather events and deforestation the physical and chemical characteristics of watersheds in mountain areas could exceed the known variations. Therefore,...
Due to extreme weather events and deforestation the physical and chemical characteristics of watersheds in mountain areas could exceed the known variations. Therefore, the spring water of the Innerste river in the Western Harz Mountains (Germany) was monitored from 2020 to 2023. The monitoring approach is simple and can be directly transferred to other watersheds of springs which are connected to the meteoric water cycle. The specific electrical conductivity (SEC) of the water is compared to daily precipitation data. The SEC values range between 50 and 100 μS/cm. In September 2020 and July 2022 the spring dried out. A hysteretic relationship between spring runoff and spring water mineralisation was found. The SEC values of the spring water are rising during dry periods or in winter seasons with a long ice cover in the catchment area. The highest SEC values are typical for long dry periods and flush events after the drying out of the spring. The relationship of SEC and major ions of the spring water (especially bicarbonate) indicates that biogeochemical processes within the soil zone and water-rock interactions in fractured shales and greywackes of the catchment area control the chemical composition of the spring water. Comparing the obtained results with data measured before the first observed drying out of the spring, no unexpected new values and correlations of runoff, SEC values and ion concentrations were found.
PubMed: 38810740
DOI: 10.1016/j.scitotenv.2024.173565