-
Journal of Oral Biology and... 2024The permanence of deep subgingival restorations are questionable both functionally and biologically. Crown lengthening is one of the traditionally performing procedures... (Review)
Review
The permanence of deep subgingival restorations are questionable both functionally and biologically. Crown lengthening is one of the traditionally performing procedures to visualize and relocate the deep margins, but the limitations of the invasive surgical procedure are anatomical complications like exposure of root concavities or furcation, violation of biological width, post operative discomfort because of sutures or periodontal packs; and less patient compliance. Other than crown lengthening, researchers tried some other techniques like modified matrix adaptation technique, using retraction cord, making holes in matrix band and flowing resin modified glass ionomer cement (RMGIC) to the root or cervical caries, orthodontic extrusion. But most of these procedures are failed to give adequate clinical success. Deep margin elevation (DME) is one of the minimally invasive and successful procedure performing in deep subgingival caries. But the evidences and knowledge in this technique is limited among practitioners. This review is to evaluate the applicability of DME, the current clinical concepts, techniques and materials for DME; and a comparison with traditionally used various techniques for cervical margin relocation also concluding that currently available various clinical parameters with this technique.
PubMed: 38481655
DOI: 10.1016/j.jobcr.2023.12.002 -
Plant Methods Nov 2023Calcium oxalate (CaOx) is the most prevalent and widespread biomineral in plants and is involved in protective and/or defensive functions against abiotic stress factors....
BACKGROUND
Calcium oxalate (CaOx) is the most prevalent and widespread biomineral in plants and is involved in protective and/or defensive functions against abiotic stress factors. It is, however, expected that this function has an extremely significant contribution to growth processes in plants bearing large amounts of CaOx, such as cacti growing in desert environment.
RESULTS
In our research, small-sized CaOx crystals (≤ 20 µm) with tetrahedral or spherical shapes were observed to dominate in each epidermal and cortical cell from the tubercles of Mammillaria schumannii, a species from the Cereoideae subfamily, having tubercles (main photosynthetic organs) united with adjacent ones almost into ridges on its stem. Because they have potential significant functions, differential centrifugations after mechanical blending were used to obtain these small-sized CaOx crystals, which extremely tend to adhere to tissue or suspend in solution. And then the combined Scanning Electron Microscope Energy Dispersive System (SEM-EDS) and Raman spectroscopy were further performed to demonstrate that the extracted crystals were mainly CaCO·2HO. Interestingly, spherical druses had 2 obvious abnormal Raman spectroscopy peaks of -CH and -OH at 2947 and 3290 cm, respectively, which may be attributed to the occluded organic matrix. The organic matrix was further extracted from spherical crystals, which could be polysaccharide, flavone, or lipid compounds on the basis of Raman spectroscopy bands at 2650, 2720, 2770, and 2958 cm.
CONCLUSIONS
Here we used a highlightedly improved method to effectively isolate small-sized CaOx crystals dominating in the epidermal and cortical cells from tubercles of Mammillaria schumannii, which extremely tended to adhere plant tissues or suspend in isolation solution. And then we further clarified the organic matrix getting involved in the formation of CaOx crystals. This improved method for isolating and characterizing biomineral crystals can be helpful to understand how CaOx crystals in cacti function against harsh environments such as strong light, high and cold temperature, and aridity.
PubMed: 38012623
DOI: 10.1186/s13007-023-01110-1 -
ACS Omega Oct 2023Anthracene core-based hole-transporting material containing TIPs (triisopropylsilylacetylene) has been spotlighted as potential donors for perovskite solar cells (SCs)...
Anthracene core-based hole-transporting material containing TIPs (triisopropylsilylacetylene) has been spotlighted as potential donors for perovskite solar cells (SCs) due to their appropriate energy levels, efficient hole transport capacity, high stability, and high power conversion efficiency. Herein, we have efficiently designed seven new highly conjugated A-B-D-C-D molecules (AS1-AS7) containing an anthracene core. We used end-capped modifications of donor units with acceptor units on one side and then theoretically characterized them for their appropriate use for SC applications. Modern quantum chemistry techniques have theoretically described the R (reference molecule) and developed (AS1-AS7) molecules. Moreover, the proposed (AS1-AS7) molecules are explored with density functional theory (DFT) and time-dependent density functional theory (TD-DFT) employing B3LYP/6-31G(d,p), and numerous parameters like photovoltaic, optical and electronic characteristics, frontier molecular orbital, excitation, binding and reorganization (λ and λ) energies, open circuit voltage, light harvesting efficiency, transition density matrix, fill factor, and the density of states have been studied. End-capped modification causes a smaller band gap between the highest occupied molecular orbital (HOMO) and lowest unoccupied molecular orbital (LUMO), higher UV-vis absorption maxima, tuned energy levels, lower binding and reorganizational (λ and λ) energies, and larger values in proposed (AS1-AS7) molecules than R. AS5 has a remarkable absorption maximum of 495.94 nm and a narrow optimal energy gap () of 1.46 eV. Furthermore, a complex study of AS5:PC61BM has revealed extraordinary charge shifting at the HOMO (AS5)-LUMO (PCBM) interface. Our results suggested that newly developed anthracene core-based compounds (AS1-AS7) would be effective candidates with excellent photovoltaic and optoelectronic properties and could be employed in future organic and perovskite SC applications.
PubMed: 37810664
DOI: 10.1021/acsomega.3c03790 -
Communications Biology Aug 2023Cells sense, manipulate and respond to their mechanical microenvironment in a plethora of physiological processes, yet the understanding of how cells transmit, receive...
Cells sense, manipulate and respond to their mechanical microenvironment in a plethora of physiological processes, yet the understanding of how cells transmit, receive and interpret environmental cues to communicate with distant cells is severely limited due to lack of tools to quantitatively infer the complex tangle of dynamic cell-cell interactions in complicated environments. We present a computational method to systematically infer and quantify long-range cell-cell force transmission through the extracellular matrix (cell-ECM-cell communication) by correlating ECM remodeling fluctuations in between communicating cells and demonstrating that these fluctuations contain sufficient information to define unique signatures that robustly distinguish between different pairs of communicating cells. We demonstrate our method with finite element simulations and live 3D imaging of fibroblasts and cancer cells embedded in fibrin gels. While previous studies relied on the formation of a visible fibrous 'band' extending between cells to inform on mechanical communication, our method detected mechanical propagation even in cases where visible bands never formed. We revealed that while contractility is required, band formation is not necessary, for cell-ECM-cell communication, and that mechanical signals propagate from one cell to another even upon massive reduction in their contractility. Our method sets the stage to measure the fundamental aspects of intercellular long-range mechanical communication in physiological contexts and may provide a new functional readout for high content 3D image-based screening. The ability to infer cell-ECM-cell communication using standard confocal microscopy holds the promise for wide use and democratizing the method.
Topics: Extracellular Matrix; Mechanical Phenomena; Fibroblasts
PubMed: 37537232
DOI: 10.1038/s42003-023-05179-1 -
International Ophthalmology Oct 2023To describe three patterns of posterior plane edge identification in small-incision lenticule extraction and to prevent lenticule mis-dissection.
PURPOSE
To describe three patterns of posterior plane edge identification in small-incision lenticule extraction and to prevent lenticule mis-dissection.
METHODS
Femtosecond laser application was performed for small-incision lenticule extraction (SMILE) by one surgeon. The surgical videos of SMILE were recorded and re-watched by the surgeon after operation.
RESULTS
Small-incision lenticule extraction was performed in 52 eyes of 28 patients, and no patient had cap-lenticular adhesion. Three patterns of posterior plane of lenticule were noticed when the surgical videos were re-watched. A "double lines" attached to the dissector were visible, signifying the reflective tape of the edge of the lenticule and the cap. During the expansion of the posterior lamellar separation, a fusiform opening between the lenticule edge and the underlying matrix layer was assumed to be a "leaf sign." With some unintentional operation, the posterior lamella was pushed away from the surgeon. The edge of the lenticule away from the anatomical part, the marking of the femtosecond laser cut, and the edge of the cap layer showed three reflective bands, which formed a "triple lines." The "double lines," "leaf sign," and "triple lines" were observed in 30 eyes (57.7%), 21 eyes (40.4%), and 1 eye (1.9%), respectively.
CONCLUSION
These three signs cover possible situations and provide visual landmarks to identify the correct dissection of the posterior plane, which can help shorten the learning curve of novice doctors.
Topics: Humans; Visual Acuity; Corneal Stroma; Corneal Surgery, Laser; Myopia; Lasers, Excimer
PubMed: 37354360
DOI: 10.1007/s10792-023-02760-9 -
Materials (Basel, Switzerland) Oct 2023In this study, the microstructure of the Mg-4Zn-4Sn-1Mn-xAl (x = 0, 0.3 wt.%, denoted as ZTM441 and ZTM441-0.3Al) as-cast alloys was investigated using scanning electron...
In this study, the microstructure of the Mg-4Zn-4Sn-1Mn-xAl (x = 0, 0.3 wt.%, denoted as ZTM441 and ZTM441-0.3Al) as-cast alloys was investigated using scanning electron microscopy (SEM), focused-ion/electron-beam (FIB) micromachining, transmission electron microscopy (TEM), and high-angle annular dark-field scanning transmission electron microscopy (HAADF-STEM). The analysis results revealed that the microstructure of the ZTM441 and ZTM441-0.3Al as-cast alloys both mainly consist of the α-Mg matrix, skeleton-shaped MgZn eutectic texture, block-shaped MgSn, and Zn/Sn-rich nanoscale precipitate bands along the grain boundary and the interdendrite. Nanoscale α-Mn dispersoids formed in the grain in the ZTM441 alloy, while no α-Mn formed in the ZTM441-0.3Al alloy instead of nanoscale AlMn particles. In the ZTM441 as-cast alloy, part of the Zn element is dissolved into the α-Mn phase, and part of the Mn element is dissolved into the MgZn phase, but in the ZTM441-0.3Al alloy, there are no such characteristics of mutual solubility. Zn and Mn elements are easy to combine in ZTM441 as-cast alloy, while Al and Mn are easy to combine in ZTM441-0.3Al as-cast alloy. The Mg-Zn phases have not only MgZn-type crystal structure but also MgZn- and MgZn-type crystal structure in the ZTM441-0.3Al as-cast alloy. The addition of Al changes the combination of Mn and Zn, promotes the formation of AlMn, and the growth of the grain.
PubMed: 37959576
DOI: 10.3390/ma16216979 -
Journal of Biomaterials Applications May 2024Self-assembling peptides (SAPs) show promise in creating synthetic microenvironments that regulate cellular function and tissue repair. Also, the precise π-π... (Review)
Review
Self-assembling peptides (SAPs) show promise in creating synthetic microenvironments that regulate cellular function and tissue repair. Also, the precise π-π interactions and hydrogen bonding within self-assembled peptide structures enable the creation of quantum confined structures, leading to reduced band gaps and the emergence of semiconductor properties within the superstructures. This review emphasizes the need for standardized 3D cell culture methods and electronic devices based on SAPs for monitoring cell communication and controlling cell surface morphology. Additionally, the gap in understanding the relationship between SAP peptide sequences and nanostructures is highlighted, underscoring the importance of optimizing peptide deposition parameters, which affect charge transport and bioactivity due to varying morphologies. The potential of peptide nanofibers as extracellular matrix mimics and the introduction of the zone casting method for improved film deposition are discussed within this review, aiming to bridge knowledge gaps and offer insights into fields like tissue engineering and materials science, with the potential for groundbreaking applications at the interface of biology and materials engineering.
Topics: Humans; Peptides; Tissue Engineering; Animals; Nanofibers; Cell Culture Techniques, Three Dimensional; Biocompatible Materials; Extracellular Matrix; Tissue Scaffolds; Electronics
PubMed: 38502905
DOI: 10.1177/08853282241240139 -
BMC Oral Health Oct 2023Proper proximal contact in direct composite restorations is crucial for periodontal health. Over a one-year period, this study was conducted to assess successive... (Randomized Controlled Trial)
Randomized Controlled Trial
Evaluation of the proximal contact tightness in class II resin composite restorations using different contact forming instruments: a 1-year randomized controlled clinical trial.
BACKGROUND
Proper proximal contact in direct composite restorations is crucial for periodontal health. Over a one-year period, this study was conducted to assess successive biological changes in proximal contact tightness PCT in class II direct composite restorations and the adjacent teeth by applying sectional matrix system along with different contact forming instruments.
METHODS
72 direct compound class II composite restorations were performed in patients aged 18-40 years and divided into 4 groups: Group I (n = 18): proximal contact was restored with Palodent plus sectional matrix system, Group II (n = 18): Trimax as contact forming instrument, Group III (n = 18): Perform as contact forming instrument and Group IV (n = 18): Contact pro as contact forming instrument. All contact forming instruments were used along with Palodent plus matrix system. PCT was measured using a digital force gauge before (T0), immediate post operative (T1) and at 3 (T2), 6 (T3), 9 (T4), and 12 months (T5) after restorative treatment. Using One-Way ANOVA, Tukey's post hoc test, and Bonferroni correction, PCT values were compared between groups before and after the intervention restoration. Meanwhile, for comparisons within groups, a paired t-test was conducted (p ≤ 0.05).
RESULTS
Contact forming instruments combined with Palodent plus sectional matrix system achieved better PCT. Trimax led to a statistically considerable tighter proximal contacts than the other groups (p < 0.05). No statistically significant difference was found in PCT between Contact pro-2, Perform and Palodent plus sectional matrix system. By means of multivariate analysis, the PCT between both T0 and T1 were increased (p < 0.001) and then it decreased till T5.
CONCLUSIONS
The use of transparent contact forming instruments achieved greater PCT compared to Palodent sectional matrix system alone that gradually decreased throughout 12 months and reached the PCT between the natural teeth. Using Trimax system provided the tightest proximal contacts. Additionally, digital force gauge was confirmed as an inclusive and accurate method to quantify PCT.
TRIAL REGISTRATION
ClinicalTrials.gov NCT05749640: 24/5/2022.
Topics: Humans; Dental Restoration, Permanent; Dental Cavity Preparation; Matrix Bands; Composite Resins; Bicuspid
PubMed: 37805456
DOI: 10.1186/s12903-023-03462-5 -
Materials (Basel, Switzerland) Dec 2023The NbTiAlZrHfTaMoW refractory high-entropy alloy (RHEA) system with the structure of the B2 matrix (antiphase domains) and antiphase domain boundaries was firstly...
The NbTiAlZrHfTaMoW refractory high-entropy alloy (RHEA) system with the structure of the B2 matrix (antiphase domains) and antiphase domain boundaries was firstly developed. We conducted the mechanical properties of the RHEAs at 298 K, 1023 K, 1123 K, and 1223 K, as well as typical deformation characteristics. The RHEAs with low density (7.41~7.51 g/cm) have excellent compressive-specific yield strength (σYS/ρ) at 1023 K (~131 MPa·cm/g) and 1123 K (~104.2 MPa·cm/g), respectively, which are far superior to most typical RHEAs. And, they still keep appropriate plastic deformability at room temperature (ε > 0.35). The superior specific yield strengths are mainly attributed to the solid solution strengthening induced by the Zr element. The formation of the dislocation slip bands with [111](101_) and [111](112_) directions and their interaction provide considerable plastic deformation capability. Meanwhile, dynamic recrystallization and dislocation annihilation accelerate the continuous softening after yielding at 1123 K.
PubMed: 38138735
DOI: 10.3390/ma16247592 -
Scientific Reports Dec 2023Spatially-structured laser beams, eventually carrying orbital angular momentum, affect electronic transitions of atoms and their motional states in a complex way. We...
Spatially-structured laser beams, eventually carrying orbital angular momentum, affect electronic transitions of atoms and their motional states in a complex way. We present a general framework, based on the spherical tensor decomposition of the interaction Hamiltonian, for computing atomic transition matrix elements for light fields of arbitrary spatial mode and polarization structures. We study both the bare electronic matrix elements, corresponding to transitions with no coupling to the atomic center-of-mass motion, as well as the matrix elements describing the coupling to the quantized atomic motion in the resolved side-band regime. We calculate the spatial dependence of electronic and motional matrix elements for tightly focused Hermite-Gaussian, Laguerre-Gaussian and for radially and azimuthally polarized beams. We show that near the diffraction limit, all these beams exhibit longitudinal fields and field gradients, which strongly affect the selection rules and could be used to tailor the light-matter interaction. The presented framework is useful for describing trapped atoms or ions in spatially-structured light fields and therefore for designing new protocols and setups in quantum optics, -sensing and -information processing. We provide open code to reproduce our results or to evaluate interaction matrix elements for different transition types, beam structures and interaction geometries.
PubMed: 38042902
DOI: 10.1038/s41598-023-48589-1