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Journal of Fungi (Basel, Switzerland) Dec 2023Citrinin (CIT), a secondary metabolite produced by the filamentous fungi species, exhibits nephrotoxic, hepatotoxic, and carcinogenic effects in mammals, remarkably...
Citrinin (CIT), a secondary metabolite produced by the filamentous fungi species, exhibits nephrotoxic, hepatotoxic, and carcinogenic effects in mammals, remarkably restricting the utilization of -derived products. CIT synthesis is mediated through the gene and modified by multiple genetic factors. Here, the regulatory effects of two transcripts, α, and β, generated via pre-mRNA alternative splicing (AS), were investigated using hairpin RNA (ihpRNA) interference, and their impact on CIT biosynthesis and the underlying mechanisms were assessed through chemical biology and transcriptome analyses. The CIT yield in ihpRNA-pksCTα and ihpRNA-pksCT (α + β) transformants decreased from 7.2 μg/mL in the wild-type strain to 3.8 μg/mL and 0.08 μg/mL, respectively. Notably, several genes in the CIT biosynthetic gene cluster, specifically , , and in the ihpRNA-pksCT (α + β) transformant, were downregulated. Transcriptome results revealed that silencing has a great impact on carbohydrate metabolism, amino acid metabolism, lipid metabolism, and AS events. The key enzymes in the citrate cycle (TCA cycle) and glycolysis were significantly inhibited in the transformants, leading to a decrease in the production of biosynthetic precursors, such as acetyl-coenzyme-A (acetyl-coA) and malonyl-coenzyme-A (malonyl-coA). Furthermore, the reduction of CIT has a regulatory effect on lipid metabolism via redirecting acetyl-coA from CIT biosynthesis towards lipid biosynthesis. These findings offer insights into the mechanisms underlying CIT biosynthesis and AS in , thus providing a foundation for future research.
PubMed: 38132775
DOI: 10.3390/jof9121174 -
Microbiology Resource Announcements Jan 2024In China, certain strains are traditionally used as edible fungi. We sequenced the genome of FM39-7 strain, an isolate from fermented rice. The genome is 25.89 Mb with...
In China, certain strains are traditionally used as edible fungi. We sequenced the genome of FM39-7 strain, an isolate from fermented rice. The genome is 25.89 Mb with a G + C content of 48.86%, containing 8485 annotated genes.
PubMed: 38099680
DOI: 10.1128/mra.00805-23 -
Journal of Fungi (Basel, Switzerland) May 2024, one of the conidiation center regulatory genes in many filamentous fungi, plays an important role in promoting asexual spores (conidia) maturation. Our recent research...
, one of the conidiation center regulatory genes in many filamentous fungi, plays an important role in promoting asexual spores (conidia) maturation. Our recent research has found that knocking out or overexpressing (a homolog of ) in M7 does not affect the development of its asexual spores like other fungi, but both repress the development of its sexual spores (ascospores). However, the mechanism remains unclear. In this study, the function of on sexual reproduction and secondary metabolism in M7 was confirmed by a complementary experiment. Moreover, the regulatory roles of in modulating the expression of genes involved in sexual reproduction, meiosis, and biosynthesis of pigment and citrinin were analyzed based on the transcriptional data. These results not only contribute to clarifying the regulation of the reproduction and secondary metabolism of spp., but also to enriching the regulation molecular mechanism of reproduction in filamentous fungi.
PubMed: 38786694
DOI: 10.3390/jof10050338