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Environmental Pollution (Barking, Essex... Oct 2023Agricultural nanotechnology has become a powerful tool to help crops and improve agricultural production in the context of a growing world population. However, its... (Review)
Review
Agricultural nanotechnology has become a powerful tool to help crops and improve agricultural production in the context of a growing world population. However, its application can have some problems with the development of harvests, especially during germination. This review evaluates nanoparticles with essential (Cu, Fe, Ni and Zn) and non-essential (Ag and Ti) elements on plant germination. In general, the effect of nanoparticles depends on several factors (dose, treatment time, application method, type of nanoparticle and plant). In addition, pH and ionic strength are relevant when applying nanoparticles to the soil. In the case of essential element nanoparticles, Fe nanoparticles show better results in improving nutrient uptake, improving germination, and the possibility of magnetic properties could favor their use in the removal of pollutants. In the case of Cu and Zn nanoparticles, they can be beneficial at low concentrations, while their excess presents toxicity and negatively affects germination. About nanoparticles of non-essential elements, both Ti and Ag nanoparticles can be helpful for nutrient uptake. However, their potential effects depend highly on the crop type, particle size and concentration. Overall, nanotechnology in agriculture is still in its early stages of development, and more research is needed to understand potential environmental and public health impacts.
Topics: Metal Nanoparticles; Silver; Agriculture; Soil; Nanoparticles; Nanotechnology; Germination
PubMed: 37482337
DOI: 10.1016/j.envpol.2023.122222 -
Plants (Basel, Switzerland) Nov 2023Flowering is a crucial process in the life cycle of most plants as it is essential for the reproductive success and genetic diversity of the species. There are... (Review)
Review
Flowering is a crucial process in the life cycle of most plants as it is essential for the reproductive success and genetic diversity of the species. There are situations in which breeders want to expedite, delay, or prevent flowering, for example, to shorten or prolong vegetative growth, to prevent unwanted pollination, to reduce the risk of diseases or pests, or to modify the plant's phenotypes. This review aims to provide an overview of the current state of knowledge to use CRISPR/Cas9, a powerful genome-editing technology to modify specific DNA sequences related to flowering induction. We discuss the underlying molecular mechanisms governing the regulation of the photoperiod, autonomous, vernalization, hormonal, sugar, aging, and temperature signal pathways regulating the flowering time. In addition, we are investigating the most effective strategies for nominating target genes. Furthermore, we have collected a dataset showing successful applications of CRISPR technology to accelerate flowering in several plant species from 2015 up to date. Finally, we explore the opportunities and challenges of using the potential of CRISPR technology in flowering time engineering.
PubMed: 38068655
DOI: 10.3390/plants12234020 -
International Journal of Biological... Jul 2023The WRKY transcription factor (TF) family, named for its iconic WRKY domain, is among the largest and most functionally diverse TF families in higher plants. WRKY TFs... (Review)
Review
The WRKY transcription factor (TF) family, named for its iconic WRKY domain, is among the largest and most functionally diverse TF families in higher plants. WRKY TFs typically interact with the W-box of the target gene promoter to activate or inhibit the expression of downstream genes; these TFs are involved in the regulation of various physiological responses. Analyses of WRKY TFs in numerous woody plant species have revealed that WRKY family members are broadly involved in plant growth and development, as well as responses to biotic and abiotic stresses. Here, we review the origin, distribution, structure, and classification of WRKY TFs, along with their mechanisms of action, the regulatory networks in which they are involved, and their biological functions in woody plants. We consider methods currently used to investigate WRKY TFs in woody plants, discuss outstanding problems, and propose several new research directions. Our objective is to understand the current progress in this field and provide new perspectives to accelerate the pace of research that enable greater exploration of the biological functions of WRKY TFs.
Topics: Humans; Transcription Factors; Plant Proteins; Plants; Stress, Physiological; Plant Development; Gene Expression Regulation, Plant; Phylogeny
PubMed: 37178519
DOI: 10.1016/j.ijbiomac.2023.124379 -
Oecologia Oct 2023Phylogenetic diversity of plant communities can influence the interaction between plants, herbivores, and their natural enemies. Plant communities with phylogenetically...
Phylogenetic diversity of plant communities can influence the interaction between plants, herbivores, and their natural enemies. Plant communities with phylogenetically distant species tend to present a wide variety of functional traits and ecological niches, which in turn can influence competitive interactions among plants as well as food and habitat quality for herbivores and their natural enemies. To assess some different mechanisms by which phylogenetic diversity of plant communities can influence herbivores and their natural enemies, we established 12 experimental plots of tropical trees with two treatments: high and low phylogenetic diversity. We measured plant growth and anti-herbivore defenses, herbivore foliar damage, and predator activity in seven species that were present in both treatments. We found significant differences in the expression of plant traits as a function of species identity and their life history, but also depending on the phylogenetic context in which they grew. Pioneer species had higher growth and produced more phenolics in plots with high phylogenetic diversity versus plants in plots with low phylogenetic diversity. Accordingly, herbivore damage in these species was greater in plots with low phylogenetic diversity. Finally, predator activity on caterpillar clay models placed on plants was greater within the low phylogenetic diversity treatment, but only for non-myrmecophytic species. These results suggest that plant phylogenetic diversity can influence the expression of growth and defensive traits and further modify the interaction between plants, herbivores, and their natural enemies. However, such effects depend on plant life history and the presence of mutualistic interaction with ants.
Topics: Phylogeny; Ecosystem; Plants; Herbivory; Plant Development
PubMed: 37777642
DOI: 10.1007/s00442-023-05455-1 -
Plant Signaling & Behavior Dec 2023, commonly known as canola, is an important oilseed crop in Canada contributing over $29.9 billion CAD to the Canadian economy annually. A major challenge facing...
, commonly known as canola, is an important oilseed crop in Canada contributing over $29.9 billion CAD to the Canadian economy annually. A major challenge facing Canadian canola is drought, which has become increasingly prevalent in recent years due to the changing climate. Research investigating novel agronomic techniques in mitigating drought is key to securing yields and sustainability in canola and other crops. One such technique is the use of bio-stimulant sprays to help offset biotic and abiotic stresses in plants through promoting stand establishment. Previous studies have shown that the application of seaweed extracts as bio-stimulant sprays to Brassicaceae has been successful in improving plant growth and development along with stress tolerance. However, this method has yet to be tested on canola. The organic nutrients that are waste products from processed seaweed help stimulate plant growth, yielding higher quality plants as a result. In association with Le Groupe Roullier, this study demonstrates that the Roullier extracts (RE) help increase plant growth characteristics and drought tolerance in canola when sprayed 3 times over a 3-week period. A high yielding but drought sensitive mutant of canola, d14 (developed through gene editing) was used for drought assays after 8 weeks of growth and where water was withheld for 6 days. Application of the REs prior to drought resulted in plants having enhanced survival rate and improved biomass retention indicating high drought tolerance. Subsequent RNA sequencing and gene ontological term analysis performed using RE treated plants in triplicates, revealed substantial levels of differential expression of growth-related genes along with stress-related genes. These REs elicited responses in plants that had previously only been achieved through gene editing and transgenic methodologies. Using bio-stimulant sprays provides a novel platform to promote beneficial agronomic traits, independent of genetic manipulation.
Topics: Plant Growth Regulators; Brassica napus; Drought Resistance; Canada; Plant Development; Droughts; Stress, Physiological
PubMed: 37903454
DOI: 10.1080/15592324.2023.2267222 -
International Journal of Molecular... Apr 2024Sulfur (S), one of the crucial macronutrients, plays a pivotal role in fundamental plant processes and the regulation of diverse metabolic pathways. Additionally, it has... (Review)
Review
Sulfur (S), one of the crucial macronutrients, plays a pivotal role in fundamental plant processes and the regulation of diverse metabolic pathways. Additionally, it has a major function in plant protection against adverse conditions by enhancing tolerance, often interacting with other molecules to counteract stresses. Despite its significance, a thorough comprehension of how plants regulate S nutrition and particularly the involvement of phytohormones in this process remains elusive. Phytohormone signaling pathways crosstalk to modulate growth and developmental programs in a multifactorial manner. Additionally, S availability regulates the growth and development of plants through molecular mechanisms intertwined with phytohormone signaling pathways. Conversely, many phytohormones influence or alter S metabolism within interconnected pathways. S metabolism is closely associated with phytohormones such as abscisic acid (ABA), auxin (AUX), brassinosteroids (BR), cytokinins (CK), ethylene (ET), gibberellic acid (GA), jasmonic acid (JA), salicylic acid (SA), and strigolactones (SL). This review provides a summary of the research concerning the impact of phytohormones on S metabolism and, conversely, how S availability affects hormonal signaling. Although numerous molecular details are yet to be fully understood, several core signaling components have been identified at the crossroads of S and major phytohormonal pathways.
Topics: Plant Growth Regulators; Sulfates; Plant Development; Abscisic Acid; Cytokinins
PubMed: 38612787
DOI: 10.3390/ijms25073978 -
Journal of Experimental Botany May 2024Legumes establish symbiosis with rhizobia, forming nitrogen-fixing nodules. The central role of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in...
Legumes establish symbiosis with rhizobia, forming nitrogen-fixing nodules. The central role of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in nodule biology has been clearly established. Recently, hydrogen sulfide (H2S) and other reactive sulfur species (RSS) have emerged as novel signaling molecules in animals and plants. A major mechanism by which ROS, RNS, and RSS fulfil their signaling role is the post-translational modification of proteins. To identify possible functions of H2S in nodule development and senescence, we used the tag-switch method to quantify changes in the persulfidation profile of common bean (Phaseolus vulgaris) nodules at different developmental stages. Proteomic analyses indicate that persulfidation plays a regulatory role in plant and bacteroid metabolism and senescence. The effect of a H2S donor on nodule functioning and on several proteins involved in ROS and RNS homeostasis was also investigated. Our results using recombinant proteins and nodulated plants support a crosstalk among H2S, ROS, and RNS, a protective function of persulfidation on redox-sensitive enzymes, and a beneficial effect of H2S on symbiotic nitrogen fixation. We conclude that the general decrease of persulfidation levels observed in plant proteins of aging nodules is one of the mechanisms that disrupt redox homeostasis leading to senescence.
Topics: Root Nodules, Plant; Plant Proteins; Phaseolus; Symbiosis; Hydrogen Sulfide; Reactive Oxygen Species; Reactive Nitrogen Species; Nitrogen Fixation; Plant Senescence
PubMed: 37952184
DOI: 10.1093/jxb/erad436 -
BioMed Research International 2023The increasing public concern over the negative impacts of chemical fertilizers and pesticides on food security and sustainability has led to exploring innovative... (Review)
Review
The increasing public concern over the negative impacts of chemical fertilizers and pesticides on food security and sustainability has led to exploring innovative methods that offer both environmental and agricultural benefits. One such innovative approach is using plant-growth-promoting bioinoculants that involve bacteria, fungi, and algae. These living microorganisms are applied to soil, seeds, or plant surfaces and can enhance plant development by increasing nutrient availability and defense against plant pathogens. However, the application of biofertilizers in the field faced many challenges and required conjunction with innovative delivering approaches. Nanotechnology has gained significant attention in recent years due to its numerous applications in various fields, such as medicine, drug development, catalysis, energy, and materials. Nanoparticles with small sizes and large surface areas (1-100 nm) have numerous potential functions. In sustainable agriculture, the development of nanochemicals has shown promise as agents for plant growth, fertilizers, and pesticides. The use of nanomaterials is being considered as a solution to control plant pests, including insects, fungi, and weeds. In the food industry, nanoparticles are used as antimicrobial agents in food packaging, with silver nanomaterials being particularly interesting. However, many nanoparticles (Ag, Fe, Cu, Si, Al, Zn, ZnO, TiO, CeO, AlO, and carbon nanotubes) have been reported to negatively affect plant growth. This review focuses on the effects of nanoparticles on beneficial plant bacteria and their ability to promote plant growth. Implementing novel sustainable strategies in agriculture, biofertilizers, and nanoparticles could be a promising solution to achieve sustainable food production while reducing the negative environmental impacts.
Topics: Fertilizers; Nanotubes, Carbon; Agriculture; Plant Development; Pesticides; Plants; Nanoparticles; Food Security
PubMed: 38075309
DOI: 10.1155/2023/6911851 -
Frontiers in Plant Science 2023The biosynthesis of auxin or indole-3-acetic acid by microorganisms has a major impact on plant-microbe interactions. Several beneficial microbiota are known to produce...
The biosynthesis of auxin or indole-3-acetic acid by microorganisms has a major impact on plant-microbe interactions. Several beneficial microbiota are known to produce auxin, which largely influences root development and growth in the host plants. Akin to findings in rhizobacteria, recent studies have confirmed the production of auxin by plant growth-promoting fungi too. Here, we show that isolate B9 produces auxin as deduced by liquid chromatography tandem-mass spectrometry analysis. Such fungal auxin is secreted and contributes directly to enhanced root and shoot development and overall plant growth in . Furthermore, auxin production by likely involves more than one tryptophan-dependent pathway. Using auxin biosynthesis inhibitor L-Kynurenine, we show that the indole-3-pyruvate pathway might be one of the key biosynthetic routes involved in such auxin production. Confocal microscopy of the Arabidopsis reporter line helped demonstrate that B9-derived auxin is biologically active and is able to significantly enhance auxin signaling in roots during such improved root growth and plant development. Furthermore, the phenotypic growth defects arising from impaired auxin signaling in Arabidopsis mutant or upon L-Kynurenine treatment of wild-type Arabidopsis seedlings could be significantly alleviated by fungus B9-derived auxin, thus suggesting its positive role in plant growth promotion. Collectively, our results provide clear evidence that the production of auxin is one of the main mechanisms involved in induction of the beneficial plant growth by
PubMed: 37745999
DOI: 10.3389/fpls.2023.1216680 -
International Journal of Molecular... Jan 2024Plant-growth-promoting rhizobacteria (PGPR) could potentially enhance photosynthesis and benefit plant growth by improving soil nutrient uptake and affecting plant... (Review)
Review
Plant-growth-promoting rhizobacteria (PGPR) could potentially enhance photosynthesis and benefit plant growth by improving soil nutrient uptake and affecting plant hormone balance. Several recent studies have unveiled a correlation between alterations in photosynthesis and host plant resistance levels. Photosynthesis provides materials and energy for plant growth and immune defense and affects defense-related signaling pathways. Photosynthetic organelles, which could be strengthened by PGPR inoculation, are key centers for defense signal biosynthesis and transmission. Although endophytic PGPRs metabolize plant photosynthates, they can increase soluble sugar levels and alternate sugar type and distribution. Soluble sugars clearly support plant growth and can act as secondary messengers under stressed conditions. Overall, carbohydrate metabolism modifications induced by PGPR may also play a key role in improving plant resistance. We provide a concise overview of current knowledge regarding PGPR-induced modulation in carbohydrate metabolism under both pathogen-infected and pathogen-free conditions. We highlight PGPR application as a cost-saving strategy amidst unpredictable pathogen pressures.
Topics: Plant Development; Plant Growth Regulators; Carbohydrate Metabolism; Alphaproteobacteria; Sugars; Defense Mechanisms
PubMed: 38338742
DOI: 10.3390/ijms25031465