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Dependence of arsenic resistance and reduction capacity of Aeromonas hydrophila on carbon substrate.Journal of Hazardous Materials Feb 2021The high toxicity and prevalence of arsenic in the environment have aroused increasing research interest in understanding the mechanisms of microbial arsenic resistance....
The high toxicity and prevalence of arsenic in the environment have aroused increasing research interest in understanding the mechanisms of microbial arsenic resistance. A wide spectrum of arsenic resistant microbes with ability of arsenic bio-transformation has been isolated from arsenic-contaminated environments. However, arsenic resistance processes and reduction abilities of microbes under various growth conditions remain poorly understood. In this work, a high correlation between the arsenic resistance and reduction ability of Aeromonas hydrophila and the carbon substrate was identified. Genome analysis suggests that the arsenic resistance system is widely present in Aeromonas genus, and the arsenic resistance was associated with the ars operon. The sensitivity of A. hydrophila to As(V) and As(III) depended heavily on the type of carbon substrate. The upregulated expression of arsA, arsB, arsD and/or downregulated expression of glpF might be responsible for the increased microbial tolerance to As(III). The As(V) reduction rate was also affected by the type of carbon substrate. Our results provide new insights into the impacts of carbon substrate on the arsenic biotoxicity as well as arsenic biotransformation processes.
Topics: Aeromonas hydrophila; Arsenic; Bacterial Proteins; Carbon; Operon
PubMed: 32768864
DOI: 10.1016/j.jhazmat.2020.123611 -
Frontiers in Immunology 2024, a gram-negative coccobacillus bacterium, can cause various infections in humans, including septic arthritis, diarrhea (traveler's diarrhea), gastroenteritis, skin and...
, a gram-negative coccobacillus bacterium, can cause various infections in humans, including septic arthritis, diarrhea (traveler's diarrhea), gastroenteritis, skin and wound infections, meningitis, fulminating septicemia, enterocolitis, peritonitis, and endocarditis. It frequently occurs in aquatic environments and readily contacts humans, leading to high infection rates. This bacterium has exhibited resistance to numerous commercial antibiotics, and no vaccine has yet been developed. Aiming to combat the alarmingly high infection rate, this study utilizes techniques to design a multi-epitope vaccine (MEV) candidate against this bacterium based on its aerolysin toxin, which is the most toxic and highly conserved virulence factor among the species. After retrieval, aerolysin was processed for B-cell and T-cell epitope mapping. Once filtered for toxicity, antigenicity, allergenicity, and solubility, the chosen epitopes were combined with an adjuvant and specific linkers to create a vaccine construct. These linkers and the adjuvant enhance the MEV's ability to elicit robust immune responses. Analyses of the predicted and improved vaccine structure revealed that 75.5%, 19.8%, and 1.3% of its amino acids occupy the most favored, additional allowed, and generously allowed regions, respectively, while its ERRAT score reached nearly 70%. Docking simulations showed the MEV exhibiting the highest interaction and binding energies (-1,023.4 kcal/mol, -923.2 kcal/mol, and -988.3 kcal/mol) with TLR-4, MHC-I, and MHC-II receptors. Further molecular dynamics simulations demonstrated the docked complexes' remarkable stability and maximum interactions, i.e., uniform RMSD, fluctuated RMSF, and lowest binding net energy. models also predict the vaccine will stimulate a variety of immunological pathways following administration. These analyses suggest the vaccine's efficacy in inducing robust immune responses against . With high solubility and no predicted allergic responses or toxicity, it appears safe for administration in both healthy and -infected individuals.
Topics: Humans; Artificial Intelligence; Aeromonas hydrophila; Diarrhea; Travel; Machine Learning; Vaccines; Epitopes, T-Lymphocyte; Adjuvants, Immunologic; Adjuvants, Pharmaceutic; Bacterial Toxins; Pore Forming Cytotoxic Proteins
PubMed: 38495891
DOI: 10.3389/fimmu.2024.1369890 -
Antimicrobial Resistance and Infection... Mar 2024Aeromonas hydrophila infections can cause gastrointestinal symptoms such as diarrhea; however, deep infections are rarely reported. Outbreaks of A. hydrophila are...
BACKGROUND
Aeromonas hydrophila infections can cause gastrointestinal symptoms such as diarrhea; however, deep infections are rarely reported. Outbreaks of A. hydrophila are reported more frequently in fish, poultry, and snakes than in humans. This study aimed to track clonal relatedness of deep infections caused by A. hydrophila using whole genome sequencing (WGS).
METHODS
We collected three isolates of A. hydrophila in July 19 to August 29, 2019, from patients that underwent spine surgery. Accurate species identification was performed using whole-genome average nucleotide identity (ANI). Antimicrobial susceptibility testing was performed using a VITEK 2 automated AST-N334 Gram-negative susceptibility card system. Antimicrobial resistance and virulence genes were identified using the Comprehensive Antibiotic Resistance Database and Virulence Factor Database VFanalyzer.
RESULTS
All three isolates were identified as A. hydrophila based on ANI and multilocus sequence typing analysis revealed that A. hydrophila belonged to a novel sequence type (ST1172). All three isolates were susceptible to amikacin and levofloxacin; however, they were resistant to piperacillin/tazobactam, ceftriaxone, cefuroxime, cefoxitin, and imipenem. Isolate 19W05620 (patient 3) showed increased ceftazidime resistance (minimum inhibitory concentration ≥ 64 µg/mL). All three isolates possessed the same chromosomally encoded β-lactamases, including bla (β-lactamase), imiH (metallo-β-lactamase), and bla (AmpC) in plasmids.
CONCLUSIONS
Our study validated the transmission of a novel carbapenem-resistant A. hydrophila sequence type (ST1172) in patients that underwent spine surgery. Control measures should be developed to prevent dissemination of A. hydrophila in the hospital setting.
Topics: Animals; Humans; Aeromonas hydrophila; Amikacin; Carbapenems; beta-Lactamases; Anti-Infective Agents
PubMed: 38433212
DOI: 10.1186/s13756-023-01339-4 -
Ecotoxicology and Environmental Safety Sep 2023Aeromonas hydrophila is a conditional pathogen impacting public hygiene and safety. Hemolysin is a virulence factor of Aeromonas hydrophila that causes erythrocyte...
Aeromonas hydrophila is a conditional pathogen impacting public hygiene and safety. Hemolysin is a virulence factor of Aeromonas hydrophila that causes erythrocyte hemolysis, yet its transcriptional response to Cyprinus rubrofuscus remains unknown. Our investigation confirmed the hemolysis of hemolysin from A. hydrophila. Serum enzyme activity was evaluated weekly after C. rubrofuscus were immunized with hemolysin Ahh1. The results showed that the hemolysin enhances the serum superoxide dismutase (SOD), lysozyme (LZM), and catalase (CAT) activity, which reached a maximum on day 14. To elucidate the molecular interaction between hemolysin from A. hydrophila and the host, we performed transcriptome sequencing on the spleen of C. rubrofuscus 14 days post hemolysin infection. The total number of clean reads was 41.37 Gb, resulting in 79,832 unigenes with an N50 length of 1863 bp. There were 1982 significantly differentially expressed genes (DEGs), including 1083 upregulated genes and 899 downregulated genes. Transcript levels of the genes, such as LA6BL, CD2, and NLRC5, were significantly downregulated, while those of IL11, IL1R2, and IL8 were dramatically upregulated. The DEGs were mainly enriched in the immune disease, viral protein interaction with cytokine and cytokine receptor, and toll-like receptor pathways, suggesting that hemolysin stimulation can activate the transcriptional responses. RT-qPCR experiments results of seven genes, IL-8, STAT2, CTSK, PRF1, CXCL9, TLR5, and SACS, showed that their expression was highly concordant with RNA-seq data. We clarified for the first time the key genes and signaling pathways response to hemolysin from A. hydrophila, which offers strategies for treating and preventing diseases.
Topics: Animals; Spleen; Aeromonas hydrophila; Hemolysin Proteins; Hemolysis; Carps
PubMed: 37591129
DOI: 10.1016/j.ecoenv.2023.115375 -
Brazilian Journal of Microbiology :... Mar 2024Sichuan is a significant aquaculture province in China, with a total aquaculture output of 1.72 × 10 tons in 2022. One of the most significant microorganisms...
Sichuan is a significant aquaculture province in China, with a total aquaculture output of 1.72 × 10 tons in 2022. One of the most significant microorganisms hurting the Sichuan aquaculture is Aeromonas hydrophila, whose genotype and antibiotic resistance are yet unknown. This study isolated a total of 64 strains of A. hydrophila from various regions during September 2019 to June 2021 within Sichuan province, China. The technique of Multi-Locus Sequence Typing (MLST) was used for the purpose of molecular typing. Meanwhile, identification of antibiotic resistance phenotype and antibiotic resistance gene was performed. The findings of the study revealed that 64 isolates exhibited 29 sequence types (ST) throughout different regions in Sichuan, with 25 of these ST types being newly identified. Notably, the ST251 emerged as the predominant sequence type responsible for the pandemic. The resistance rate of isolated strains to roxithromycin was as high as 98.3%, followed by co-trimoxazole (87.5%), sulfafurazole (87.5%), imipenem (80%), amoxicillin (60%), and clindamycin (57.8%). Fifteen strains of A. hydrophila exhibited resistance to medicines across a minimum of three categories, suggesting the development of multidrug resistance in these isolates. A total of 63 ARGs were detected from the isolates, which mediated a range of antibiotic resistance mechanisms, with deactivation and efflux potentially serving as the primary mechanisms of antibiotic resistance. This study revealed the diversity of A. hydrophila genotypes and the risk of antibiotic resistance in Sichuan, providing reference for scientific and effective control of A. hydrophila infection.
Topics: Aeromonas hydrophila; Multilocus Sequence Typing; Drug Resistance, Microbial; Genotype; Phenotype; Anti-Bacterial Agents; China; Aeromonas
PubMed: 37999911
DOI: 10.1007/s42770-023-01187-9 -
Frontiers in Immunology 2024, a bacterium widely distributed in the natural environment, causes multiple diseases in various animals. Exploring the mechanism of the host defense against can help...
Integrated time-series biochemical, transcriptomic, and metabolomic analyses reveal key metabolites and signaling pathways in the liver of the Chinese soft-shelled turtle () against infection.
INTRODUCTION
, a bacterium widely distributed in the natural environment, causes multiple diseases in various animals. Exploring the mechanism of the host defense against can help develop efficient strategies against infection.
METHODS
Herein, we investigated the temporal influence of A. hydrophila on the Chinese soft-shelled turtle, an economically important species, at the biochemical, transcriptomic, and metabolomic levels. Plasma parameters were detected with the test kits. Transcriptome and metabolome were respectively applied to screen the differentially expressed genes and metabolites.
RESULTS
The contents or activities of these plasma parameters were significantly increased at 24 hpi and declined at 96 hpi, indicating that 24 and 96 hpi were two important time points during infection. Totals of 3121 and 274 differentially expressed genes (DEGs) from the transcriptome while 74 and 91 differentially abundant metabolites (DAMs) from the metabolome were detected at 24 and 96 hpi. The top DEGs at 24 hpi included and while and were the most abundant at 96 hpi. The predominant DAMs included O-phospho-L-serine, γ-Aminobutyric acid, orotate, L-tyrosine, and L-tryptophan at 24 hpi, as well as L-glutamic acid, L-arginine, glutathione, glutathione disulfide, and citric acid at 96 hpi.
DISCUSSION
The combined analysis of DEGs and DAMs revealed that tryptophan metabolism, nicotinate and nicotinamide metabolism, as well as starch and sucrose metabolism, were the most important signaling pathways at the early infective stage while tyrosine metabolism, pyrimidine metabolism, as well as alanine, aspartate and glutamate metabolism were the most crucial pathways at the later stage. In general, our results indicated that the Chinese soft-shelled turtle displays stage-specific physiological responses to resist infection.
Topics: Animals; Turtles; Aeromonas hydrophila; Gram-Negative Bacterial Infections; Signal Transduction; Metabolomics; Liver; Metabolome; Transcriptome; Gene Expression Profiling
PubMed: 38799475
DOI: 10.3389/fimmu.2024.1376860 -
International Microbiology : the... Aug 2022Aeromonas hydrophila is a common pathogen in fish that has caused severe economic losses in aquaculture worldwide. With the emergence of bacterial resistance, it is...
Aeromonas hydrophila is a common pathogen in fish that has caused severe economic losses in aquaculture worldwide. With the emergence of bacterial resistance, it is necessary to develop new drugs to combat bacterial infection, particularly for multidrug-resistant bacteria. In this study, the antibacterial activity of pinocembrin was investigated by observing bacterial growth and microscopic structure, and its mechanism of action was identified by investigating its effect on protein and DNA. The antibacterial susceptibility test indicated that pinocembrin inhibits A. hydrophila growth. The minimal inhibitory concentration and minimum bactericidal concentration were 256 μg/mL and 512 μg/mL, respectively. Ultrastructurally, the bacteria treated with pinocembrin showed surface roughness and plasmolysis. When bacteria were treated with 512 μg/mL pinocembrin, lactate dehydrogenase activity and soluble protein content decreased significantly, and electrical conductivity and DNA exosmosis levels increased by 4.21 ± 0.64% and 15.98 ± 1.93 mg/L, respectively. Staining with 4', 6-Diamidino-2-phenylindole showed that the nucleic acid fluorescence intensity and density decreased after the treatment with pinocembrin. Pinocembrin may inhibit the growth of A. hydrophila by increasing cell membrane permeability and affecting protein and DNA metabolism. Thus, pinocembrin is a candidate drug for the treatment of A. hydrophila infection in aquaculture.
Topics: Aeromonas hydrophila; Animals; Anti-Bacterial Agents; Fish Diseases; Flavanones; Microbial Sensitivity Tests
PubMed: 35438439
DOI: 10.1007/s10123-022-00245-w -
Biocontrol Science 2021Aeromonas hydrophila is a major waterborne pathogen, which induces various diseases in freshwater fish with the capability for zoonotic potential. This study was applied...
Virulent and Multiple Antimicrobial Resistance Aeromonas hydrophila Isolated from Diseased Nile Tilapia Fish (Oreochromis niloticus) in Egypt with Sequencing of Some Virulence-Associated Genes.
Aeromonas hydrophila is a major waterborne pathogen, which induces various diseases in freshwater fish with the capability for zoonotic potential. This study was applied to investigate the prevalence of A. hydrophila in diseased Nile tilapia fish, genetic characterization of the virulence encoding genes (act, aerA, alt, and ast genes), and antibiotic susceptibility. Out of the 500 diseased Nile tilapia fish samples, 70% (350/500) Aeromonas species were isolated. From which 53.4% (187/350) of Aeromonas hydrophila strains were identified. A. hydrophila was detected in kidneys, followed by liver, spleen, intestine, and gills. The results of virulotyping displayed the presence of act, and aerA genes in a high percentage of 40%, followed by alt gene (30%), but ast gene was not detected (0%) in A. hydrophila strains. Based on DNA sequence analysis of three virulence associated-genes (act, aerA, and alt genes), the phylogenetic tree showed the genetic relationship with related species. Finally, the antibiotic susceptibility tests revealed high resistance toward chloramphenicol (67.4%), followed by amikacin (51.9%) and gentamicin (47.1%), whereas a high sensitivity was exhibited toward meropenem (90.9%), followed by ciprofloxacin (84.2%), amoxicillin-clavulanic acid (73.3%) and trimethoprim-sulfamethoxazole (64.2%). The multidrug-resistant A. hydrophila strains were observed in 69.0% of strains with six resistance patterns.
Topics: Aeromonas hydrophila; Animals; Anti-Bacterial Agents; Cichlids; Drug Resistance, Bacterial; Egypt; Fish Diseases; Phylogeny; Virulence
PubMed: 34556619
DOI: 10.4265/bio.26.167 -
Journal of Food Protection Mar 2024Aquaponic production of fresh produce is a sustainable agricultural method becoming widely adopted, though few studies have investigated potential food safety hazards...
Aquaponic production of fresh produce is a sustainable agricultural method becoming widely adopted, though few studies have investigated potential food safety hazards within commercial systems. A longitudinal study was conducted to isolate and quantify several foodborne pathogens from a commercial, aquaponic farm, and to elucidate their distribution throughout. The survey was conducted over 2 years on a controlled-environment farm containing Nile tilapia (Oreochromis niloticus) and lettuce (Lactuca sativa). Samples (N = 1,047) were collected bimonthly from three identical, independent systems, and included lettuce leaves, roots, fingerlings (7-126 d old), feces from mature fish (>126 d old), water, and sponge swabs collected from the tank interior surface. Most probable number of generic Escherichia coli were determined using IDEXX Colilert Quanti-Tray. Enumeration and enrichment were used to detect Shiga toxin-producing E. coli (STEC), Salmonella enterica, Listeria monocytogenes, Aeromonas spp., Aeromonas hydrophilia, and Pseudomonas aeruginosa. Generic E. coli, STEC, L. monocytogenes, and S. enterica were not detected in collected samples. P. aeruginosa was isolated from water (7/351; 1.99%), swabs (3/351; 0.85%), feces (2/108; 1.85%), and lettuce leaves (2/99; 2.02%). A. hydrophila was isolated from all sample types (623/1047; 59.50%). The incidence of A. hydrophila in water (X = 23.234, p < 0.001) and sponge samples (X = 21.352, p < 0.001) increased over time.
Topics: Animals; Escherichia coli; Aeromonas hydrophila; Longitudinal Studies; Agriculture; Water
PubMed: 38278488
DOI: 10.1016/j.jfp.2024.100230 -
MSphere Aug 2022Bacterial pathogens are well equipped to adhere to and initiate infection in teleost fish. Fish skin mucus serves as the first barrier against environmental pathogens....
Bacterial pathogens are well equipped to adhere to and initiate infection in teleost fish. Fish skin mucus serves as the first barrier against environmental pathogens. The mucus harbors commensal microbes that impact host physiological and immunological responses. However, how the skin mucosal microbiota responds to the presence of pathogens remains largely unexplored. Thus, little is known about the status of skin mucus prior to infection with noticeable symptoms. In this study, we investigated the interactions between pathogens and the skin mucosal microbiota as well as the fish skin immune responses in the presence of pathogens. Striped catfish (Pangasianodon hypophthalmus) were challenged with different concentrations of the bacterial pathogen Aeromonas hydrophila (AH), and the skin immune response and the mucosal microbiota were examined by quantitative PCR (qPCR) and 16S rRNA gene sequence analysis. We determined that the pathogen concentration needed to stimulate the skin immune response was associated with significant mucosal microbiota changes, and we reconfirmed these observations using an fish skin model. Further analysis indicated that changes in the microbiota were attributed to a significant increase in opportunistic pathogens over AH. We concluded that the presence and increase of AH result in dysbiosis of the mucosal microbiota that can stimulate skin immune responses. We believe that our work sheds light on host-pathogen-commensal microbiota interactions and therefore contributes to aquaculture fish health. The fish skin mucosal microbiota is essential in modulating the host response to the presence of pathogens. Our study provides a platform to study both the correlation and causation of the interactions among the pathogen, fish skin, and the skin mucosal microbiota. Based on these findings, we provide the first mechanistic information on how mucosal microbiota changes induced by the pathogen AH result in skin disturbance with immune stimulation in striped catfish in the natural state and a potential direction for early-infection screening. Thus, this study is highly significant in the prevention of fish disease.
Topics: Aeromonas hydrophila; Animals; Catfishes; Dysbiosis; Microbiota; RNA, Ribosomal, 16S
PubMed: 35766485
DOI: 10.1128/msphere.00194-22