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Experimental Dermatology May 2022The present account offers a generalized view of the evolution of process of terminal differentiation in keratinocytes of the epidermis in anamniotes, indicated as...
The present account offers a generalized view of the evolution of process of terminal differentiation in keratinocytes of the epidermis in anamniotes, indicated as keratinization, into a further differentiating process of cornification in the skin and appendages of terrestrial vertebrates. Keratinization indicates the prevalent accumulation of intermediate filaments of keratins (IFKs) and is present in most fish and amphibian epidermis and inner epithelia of all vertebrates. During land adaptation, terrestrial vertebrates evolved a process of cornification and keratinocytes became dead corneocytes by the addition of numerous others proteins to the IFKs framework, represented by keratin-associated proteins (KAPs) and corneous proteins (CPs). Most of genes coding for these types of proteins are localized in chromosomal loci different and un-related from those of IFKs, and CPs originated from a gene cluster indicated as epidermal differentiation complex. During the evolution of reptiles and birds, the epidermis and corneous derivatives such as scales, claws, beaks and feathers mainly accumulate a type of CPs that overcome IFKs and containing a 34 amino acid beta-sheet core indicated as corneous beta proteins, formerly known as beta-keratins. Mammals did not evolve a beta-sheet core in their CPs and KAPs but instead produced numerous cysteine-rich IFKs in their epidermis and specialized KAPs in hairs, claws, nails, hooves and horns.
Topics: Amphibians; Animals; Epidermis; Keratins; Mammals; Reptiles; Vertebrates
PubMed: 35007368
DOI: 10.1111/exd.14525 -
Integrative Zoology Sep 2022Individual age and population age composition are the major concerns of ecologists, evolutionary biologists and conservation biologists. In amphibians,... (Review)
Review
Individual age and population age composition are the major concerns of ecologists, evolutionary biologists and conservation biologists. In amphibians, skeletochronology-counting the number of lines of arrested growth deposited in the bone tissue, is the dominant method to determine actual age of an individual. Since 1970s, age data of the ectothermic taxa have been accumulated and increasingly used in comparative studies. Here we make a global assessment for the availability of the data, based on a collection of 369 published papers. For a specific species, more males than females were sampled. Among the extant 8146 amphibian species, only 266 (3.3%) have been skeletochronologically investigated. Of these studied species, 2 (0.9% of 214) belong to caecilians, 56 (7.6% of 740) salamanders and 208 (2.9% of 7192) anurans. A complete paucity of data was seen in 80%, 50%, and 54% of families in the corresponding orders. More temperate species than tropical species were sampled, while the proportion of Palearctic species studied was higher than that in the other 5 biogeographical realms. Species inhabiting semi-aquatic niche were more likely to be studied than fossorial or plant dwellers. Age information of multiple populations (2-48) was available for species with a broad distribution, accounts for 61% of salamander and 43% of anuran species studied. Because these gaps in demographic knowledge can limit our understanding of questions ranging from life history evolution, population dynamics to conservation, we encourage herpetologists to pay more efforts on filling them.
Topics: Amphibians; Animals; Anura; Female; Male; Population Dynamics
PubMed: 34498379
DOI: 10.1111/1749-4877.12584 -
PloS One 2019Amphibians are the focus of a recent debate and public attention owing to the global decline in their populations worldwide. Amphibians are one of the most threatened...
Amphibians are the focus of a recent debate and public attention owing to the global decline in their populations worldwide. Amphibians are one of the most threatened and poorly known groups of vertebrates in several geographic areas, even though they play a central role in their own ecosystems. At different levels, amphibians make their contribution to proper ecosystem functioning. They act as regulators of the food web and nutrient cycling, and they also provide several valuable ecosystem services, e.g., as a food source and as animal models for lab research. In this sense, it seems clear that the maintenance of amphibian diversity should be one of the major goals for the several countries where their population decline is observed. However, we are still struggling with the very first step of this process, i.e., the correct identification of the amphibian species diversity. Over the past few decades, research on molecular identification of amphibians using DNA barcoding has encountered some difficulties related to high variability in the mitochondrial genome of amphibians, and a research gap is noticeable in the literature. We herein evaluated both COI and 16S rRNA mitochondrial genes for the molecular identification of frogs and tadpoles in a large fragment of the South American Atlantic Rainforest in Rio de Janeiro, Brazil. Our results suggest that both COI and 16S rRNA are informative markers for the molecular identification of the amphibian specimens with all specimens unambiguously identified at the species level. We also made publicly available 12 new sequences of Atlantic Rainforest amphibian species for the first time, and we discussed some conservation issues related to amphibians within the Atlantic Rainforest domains in the state of Rio de Janeiro, Brazil.
Topics: Amphibians; Animals; Atlantic Ocean; Conservation of Natural Resources; Larva; Molecular Biology; RNA, Ribosomal, 16S; Rainforest; South America
PubMed: 31644600
DOI: 10.1371/journal.pone.0224320 -
Methods in Molecular Biology (Clifton,... 2023Assessing numbers of leukocytes in salamanders and other amphibians can be useful metrics for understanding health or stress levels of individuals in a population. In...
Assessing numbers of leukocytes in salamanders and other amphibians can be useful metrics for understanding health or stress levels of individuals in a population. In this chapter we describe the procedures for obtaining blood samples from amphibians, preparing blood films for microscopy, counting, and identifying cells. We also provide reference values for amphibian leukocytes for use in interpreting leukocyte data. From our assessment of the published and unpublished literature, "non-stressed" salamanders would have a leukocyte profile where 60-70% of cells are lymphocytes, 17-30% are neutrophils, 1-4% are eosinophils, 4-12% are basophils, and 2-6% are monocytes. In Ambystoma spp., the eosinophil abundance can be notably higher (30% of all white blood cells), for reasons unknown. Finally, the neutrophil-lymphocyte ratio of most non-stressed salamanders tends to be between 0.3 and 0.4 (sometimes less), while the ratios of stressed salamanders tend to be over 1.0.
Topics: Animals; Humans; Urodela; Leukocytes; Leukocyte Count; Ambystoma; Neutrophils; Amphibians; Eosinophils
PubMed: 36272093
DOI: 10.1007/978-1-0716-2659-7_29 -
Science (New York, N.Y.) Sep 2022
Topics: Amphibians; Animals; Classification; Genomics
PubMed: 36108017
DOI: 10.1126/science.ade5002 -
Hormones and Behavior Feb 2020Research on hormonal involvement in animals' preferences for mating or pairing with same- or other-sex partners has been among the topics featured in Hormones and... (Review)
Review
Research on hormonal involvement in animals' preferences for mating or pairing with same- or other-sex partners has been among the topics featured in Hormones and Behavior over the years. In several species of non-pair-forming mammals, there is good evidence that the early sex steroid environment has an organizational effect on later sexual partner preference. Research with zebra finches, a pair-forming species, shows a likely early estrogenic organizational hormone effect on pairing partner preference, an effect that can also interact with the early social environment to determine adult pairing preference. Experiments with two amphibian and fish species suggest that activational hormone effects (effects of the hormone milieu in adulthood) may regulate sexual partner preference. As a complement to the research on hormonal involvement, a growing body of theoretical and empirical research addresses the possible adaptive functions of the same-sex sexual and pairing behavior observed in many wild animals. Such advances have important implications for conceptualizing neuroendocrine mechanisms of partner preferences.
Topics: Amphibians; Animals; Birds; Female; Fishes; Gonadal Steroid Hormones; Male; Pair Bond; Sexual Behavior, Animal; Social Environment; Songbirds
PubMed: 31778718
DOI: 10.1016/j.yhbeh.2019.104646 -
EcoHealth Sep 2020Infection records of Batrachochytrium dendrobatidis (Bd), a pathogen that has devastated amphibian populations worldwide, have rapidly increased since the pathogen's...
Infection records of Batrachochytrium dendrobatidis (Bd), a pathogen that has devastated amphibian populations worldwide, have rapidly increased since the pathogen's discovery. Dealing with so many records makes it difficult to (a) know where, when and in which species infections have been detected, (b) understand how widespread and pervasive Bd is and (c) prioritize study and management areas. We conducted a systematic review of papers and compiled a database with Bd infection records. Our dataset covers 71 amphibian families and 119 countries. The data revealed how widespread and adaptable Bd is, being able to infect over 50% of all tested amphibian species, with over 1000 confirmed host species and being present in 86 countries. The distribution of infected species is uneven among and within countries. Areas where the distributions of many infected species overlap are readily visible; these are regions where Bd likely develops well. Conversely, areas where the distributions of species that tested negative overlap, such as the Atlantic Coast in the USA, suggest the presence of Bd refuges. Finally, we report how the number of tested and infected species has changed through time, and provide a list of oldest detection records per country.
Topics: Amphibians; Animals; Chytridiomycota; Communicable Diseases, Emerging; Databases as Topic; Internationality
PubMed: 33201333
DOI: 10.1007/s10393-020-01504-w -
Conservation Biology : the Journal of... Feb 2021Invasive species have major impacts on biodiversity and are one of the primary causes of amphibian decline and extinction. Unlike other top ant invaders that negatively...
Invasive species have major impacts on biodiversity and are one of the primary causes of amphibian decline and extinction. Unlike other top ant invaders that negatively affect larger fauna via chemical defensive compounds, the Argentine ant (Linepithema humile) does not have a functional sting. Nonetheless, it deploys defensive compounds against competitors and adversaries. We estimated levels of ant aggression toward 3 native terrestrial amphibians by challenging juveniles in field ant trails and in lab ant foraging arenas. We measured the composition and quantities of toxin in L. humile by analyzing pygidial glands and whole-body contents. We examined the mechanisms of toxicity in juvenile amphibians by quantifying the toxin in amphibian tissues, searching for histological damages, and calculating toxic doses for each amphibian species. To determine the potential scope of the threat to amphibians, we used global databases to estimate the number, ranges, and conservation status of terrestrial amphibian species with ranges that overlap those of L. humile. Juvenile amphibians co-occurring spatially and temporally with L. humile die when they encounter L. humile on an ant trail. In the lab, when a juvenile amphibian came in contact with L. humile the ants reacted quickly to spray pygidial-gland venom onto the juveniles. Iridomyrmecin was the toxic compound in the spray. Following absorption, it accumulated in brain, kidney, and liver tissue. Toxic dose for amphibian was species dependent. Worldwide, an estimated 817 terrestrial amphibian species overlap in range with L. humile, and 6.2% of them are classified as threatened. Our findings highlight the high potential of L. humile venom to negatively affect amphibian juveniles and provide a basis for exploring the largely overlooked impacts this ant has in its wide invasive range.
Topics: Amphibians; Animals; Ant Venoms; Ants; Behavior, Animal; Conservation of Natural Resources
PubMed: 32812277
DOI: 10.1111/cobi.13604 -
Journal of Experimental Zoology. Part... Jan 2021Ectothermic animals, such as amphibians and reptiles, are particularly sensitive to rapidly warming global temperatures. One response in these organisms may be to evolve... (Review)
Review
Ectothermic animals, such as amphibians and reptiles, are particularly sensitive to rapidly warming global temperatures. One response in these organisms may be to evolve aspects of their thermal physiology. If this response is adaptive and can occur on the appropriate time scale, it may facilitate population or species persistence in the changed environments. However, thermal physiological traits have classically been thought to evolve too slowly to keep pace with environmental change in longer-lived vertebrates. Even as empirical work of the mid-20th century offers mixed support for conservatism in thermal physiological traits, the generalization of low evolutionary potential in thermal traits is commonly invoked. Here, we revisit this hypothesis to better understand the mechanisms guiding the timing and patterns of physiological evolution. Characterizing the potential interactions among evolution, plasticity, behavior, and ontogenetic shifts in thermal physiology is critical for accurate prediction of how organisms will respond to our rapidly warming world. Recent work provides evidence that thermal physiological traits are not as evolutionarily rigid as once believed, with many examples of divergence in several aspects of thermal physiology at multiple phylogenetic scales. However, slow rates of evolution are often still observed, particularly at the warm end of the thermal performance curve. Furthermore, the context-specificity of many responses makes broad generalizations about the potential evolvability of traits tenuous. We outline potential factors and considerations that require closer scrutiny to understand and predict reptile and amphibian evolutionary responses to climate change, particularly regarding the underlying genetic architecture facilitating or limiting thermal evolution.
Topics: Adaptation, Physiological; Amphibians; Animals; Body Temperature Regulation; Reptiles
PubMed: 32970931
DOI: 10.1002/jez.2414 -
Physiological and Biochemical Zoology :... 2023AbstractTrade-offs between life history traits are context dependent; they vary depending on environment and life stage. Negative associations between development and...
AbstractTrade-offs between life history traits are context dependent; they vary depending on environment and life stage. Negative associations between development and growth often characterize larval life stages. Both growth and development consume large parts of the energy budget of young animals. The metabolic rate of animals should reflect differences in growth and developmental rates. Growth and development can also have negative associations with immune function because of their costs. We investigated how intraspecific variation in growth and development affected the metabolism of larval amphibians and whether intraspecific variation in growth, development, and metabolic rate could predict mortality and viral load in larvae infected with ranavirus. We also compared the relationship between growth and development before and after infection with ranavirus. We hypothesized that growth and development would affect metabolism and predicted that each would have a positive correlation with metabolic rate. We further hypothesized that allocation toward growth and development would increase ranavirus susceptibility and therefore predicted that larvae with faster growth, faster development, and higher metabolic rates would be more likely to die from ranavirus and have higher viral loads. Finally, we predicted that growth rate and developmental rate would have a negative association. Intraspecific variation in growth rate and developmental rate did not affect metabolism. Growth rate, developmental rate, and metabolism did not predict mortality from ranavirus or viral load. Larvae infected with ranavirus exhibited a trade-off between developmental rate and growth rate that was absent in uninfected larvae. Our results indicate a cost of ranavirus infection that is potentially due to both the infection-induced anorexia and the cost of infection altering priority rules for resource allocation.
Topics: Animals; DNA Virus Infections; Larva; Ranavirus; Life History Traits; Amphibians
PubMed: 38237190
DOI: 10.1086/727729