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International Journal of Systematic and... Sep 2023A novel, anaerobic, Gram-stain-positive coccoid strain, CBA3646, was isolated from the faeces of a thoroughbred racehorse. Phylogenetic analysis based on 16S rRNA gene...
A novel, anaerobic, Gram-stain-positive coccoid strain, CBA3646, was isolated from the faeces of a thoroughbred racehorse. Phylogenetic analysis based on 16S rRNA gene sequencing yielded results indicative of CBA3646 representing a member of the genus , with the species most closely related to it being DSM 20463, with a similarity of 94.79 %. DNA-DNA relatedness and average nucleotide identity values between CBA3646 and DSM 20463 were 21.4 and 67.6 %, respectively. CBA3646 has a circular chromosomal genome of 1 709 189 bp (45.5 mol% DNA G+C content), containing 1652 genes in total, 1584 predicted protein-coding genes, 3 complete rRNA loci and 47 tRNA genes. The cells were non-motile diplococci, catalase-positive and oxidase-negative. Growth of CBA3646 was observed at 20-40 °C (optimal temperature, 35 °C) and in the presence of 0-4 % (w/v) NaCl (optimum concentration, 1 %). The major fatty acids (>10 %) of CBA3646 were C, Cω9 and Cω9 dimethyl acetal, with its major polar lipids being diphosphatidylglycerol and phosphatidylglycerol. The elucidated phylogenetic, physiological, chemotaxonomic and molecular properties are indicative of strain CBA3646 representing a novel species of the genus , or which the name sp. nov. is proposed. The type strain is CBA3646 (= KACC 22890 = JCM 35845).
Topics: Horses; Animals; Coloring Agents; Anaerobiosis; Base Composition; Fatty Acids; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; DNA, Bacterial; Bacterial Typing Techniques; Gram-Positive Cocci; Feces; Clostridiales
PubMed: 37750780
DOI: 10.1099/ijsem.0.006053 -
Archives of Microbiology Dec 2021A Gram-stain-positive, coccus-shaped, facultatively anaerobic, non-motile bacterial strain, designated YIM S02567, was isolated from a forest soil sample collected from...
Propioniciclava soli sp. nov., isolated from forest soil, Yunnan, China, and reclassification of the genus Brevilactibacter into the genus Propioniciclava, and Brevilactibacter sinopodophylli, Brevilactibacter flavus, and Brevilactibacter coleopterorum as Propioniciclava sinopodophylli comb. nov.,...
A Gram-stain-positive, coccus-shaped, facultatively anaerobic, non-motile bacterial strain, designated YIM S02567, was isolated from a forest soil sample collected from Gejiu City, Yunnan Province, southwest PR China. Growth was observed at 10-45 °C, at pH 6.0-9.5, in the presence of up to 4.0% (w/v) NaCl on R2A medium. The results of 16S rRNA gene sequence similarity analysis showed that strain YIM S02567 was most closely related to the type strain of Brevilactibacter sinopodophylli (95.4%) and Propioniciclava tarda (94.7%), and phylogenetic analysis based on genome data showed that strain YIM S02567 should be assigned to the genus Propioniciclava. The cell-wall diamino acid was meso-diaminopimelic acid. The major cellular fatty acids were identified as anteiso-C and C, and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, and two unidentified glycolipids. The predominant menaquinone was MK-9(H). The genomic DNA G + C content was 71.2 mol%. Based on the polyphasic taxonomic evidence, strain YIM S02567 is assigned to a novel member of the genus Propioniciclava, for which the name Propioniciclava soli sp. nov., (type strain YIM S02567 = CCTCC AB 2020128 = CGMCC 1.18504 = KCTC 49478) is proposed. Furthermore, we propose the reclassification of Brevilactibacter as Propioniciclava gen. nov.
Topics: China; Forests; Phylogeny; Propionibacteriaceae; RNA, Ribosomal, 16S; Soil
PubMed: 34928401
DOI: 10.1007/s00203-021-02621-y -
International Journal of Systematic and... Jan 2021A polyphasic taxonomic approach was used to characterize a novel bacterium, designated as strain HDW20, isolated from the intestine of the dark diving beetle . The...
A polyphasic taxonomic approach was used to characterize a novel bacterium, designated as strain HDW20, isolated from the intestine of the dark diving beetle . The isolate was Gram-stain-positive, facultatively anaerobic, non-motile, coccus-shaped, and formed pale orange colonies. Phylogenetic analysis based on 16S rRNA gene sequences and genome sequences showed that the isolate belonged to the genus in the phylum and was closely related to SST-39, JCM 17540, and NSG39, with the highest 16S rRNA gene sequence similarity of 98.5 % and a highest average nucleotide identity (ANI) value of 80.6 %. The major cellular fatty acids were C 9 and anteiso-C. The main respiratory quinone was MK-9 (H). The major polar lipid components were phosphatidylglycerol and diphosphatidylglycerol. The genomic DNA G+C content was 69.0 %. The isolate contains ʟʟ-diaminopimelic acid, ʟ-alanine, and ʟ-lysine as amino acid components, and ribose, glucose, and galactose as sugar components of the cell wall peptidoglycan. The results of phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses suggested that strain HDW20 represents a novel species within the genus . We propose the name sp. nov. The type strain is HDW20 (=KACC 21348=KCTC 49324=JCM 33674).
Topics: Animals; Bacterial Typing Techniques; Base Composition; Coleoptera; DNA, Bacterial; Diaminopimelic Acid; Fatty Acids; Intestines; Peptidoglycan; Phospholipids; Phylogeny; Pigmentation; Propionibacteriaceae; RNA, Ribosomal, 16S; Republic of Korea; Rivers; Sequence Analysis, DNA; Vitamin K 2
PubMed: 33289624
DOI: 10.1099/ijsem.0.004588 -
Antonie Van Leeuwenhoek Aug 2021A Gram-stain-positive, non-pigmented, coccus-shaped, facultatively anaerobic and α-hemolytic bacterium designated as INB8 was isolated from a hypersaline marine water...
Reclassification of Facklamia ignava, Facklamia sourekii and Facklamia tabacinasalis as Falseniella ignava gen. nov., comb. nov., Hutsoniella sourekii gen. nov., comb. nov., and Ruoffia tabacinasalis gen. nov., comb. nov., and description of Ruoffia halotolerans sp. nov., isolated from hypersaline...
A Gram-stain-positive, non-pigmented, coccus-shaped, facultatively anaerobic and α-hemolytic bacterium designated as INB8 was isolated from a hypersaline marine water sample collected at the Inland Sea of Qatar. The isolate was able to grow at 25-40 °C (optimum, 30 °C), at pH 5-11 and with 2-8% NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain INB8 was placed within the family Aerococcaceae with the highest sequence similarity to Facklamia tabacinasalis CCUG 30090 (99.5%), followed by Facklamia hominis CCUG 36813 (93.9%), Facklamia sourekii Y17312 (93.8%), Facklamia ignava CCUG 37419 (93.6%), Facklamia miroungae CCUG 42728 (93.5%), Suicoccus acidiformans ZY16052 (93.5%), Facklamia languida CCUG 37842 (93.2%), Ignavigranum ruoffiae (93.1%), and Dolosicoccus paucivorans DSM 15742 (90.8%). Average nucleotide identity and digital DNA-DNA hybridization values between strain INB8 and F. tabacinasalis CCUG 30090 were determined to be 94.5% and 58.9% respectively, confirming strain INB8 represents a novel species. The major fatty acids were C C C and C ω9c The G + C content of strain INB8 determined from the genome was 36.3 mol%. Based on the phylogenetic, chemotaxonomic and phenotypic information, it is proposed that Facklamia tabacinasalis should be reclassified as Ruoffia tabacinasalis, Facklamia ignava be reclassified as Falseniella ignava, and Facklamia sourekii be reclassified Hutsoniella sourekii. It is further proposed that strain INB8 should be classified as a species of the genus Ruoffia for which the name Ruoffia halotolerans sp. nov. is proposed. The type strain is INB8 (= LMG 30291 = CCUG 70701 = QCC/B60/17).
Topics: Aerococcaceae; Bacterial Typing Techniques; DNA, Bacterial; Phospholipids; Phylogeny; Qatar; RNA, Ribosomal, 16S; Sequence Analysis, DNA
PubMed: 34181136
DOI: 10.1007/s10482-021-01587-7 -
Current Microbiology Oct 2019A novel facultative anaerobic, non-spore forming, non-motile, and Gram-stain-positive coccus, designated strain ChDC B353, was isolated from human postoperative...
A novel facultative anaerobic, non-spore forming, non-motile, and Gram-stain-positive coccus, designated strain ChDC B353, was isolated from human postoperative maxillary cyst. The 16S ribosomal RNA gene (16S rDNA) sequence of the strain was most closely related to those of Streptococcus pseudopneumoniae ATCC BAA-960 (99.4%), Streptococcus mitis NCTC 12261 (99.3%), and Streptococcus pneumoniae NCTC 7465 (99.2%). The major fatty acids of the strain were C (43.2%) and C/C (20.2%). The genome of strain ChDC B353 was composed of 1,902,053 bps. The DNA G+C content of the strain was 40.2 mol%. Average nucleotide identity (ANI) values between strain ChDC B353 and S. pseudopneumoniae ATCC BAA-960, S. mitis NCTC 12261, and S. pneumoniae NCTC 7465 were 91.9%, 93.5%, and 91.3%, respectively. Genome-to-genome distance (GGD) values between strain ChDC B353 and S. pseudopneumoniae ATCC BAA-960, S. mitis NCTC 12261, or S. pneumoniae NCTC 7465 were 46.6% (44.0-49.2%), 53.2% (50.5-55.9%), and 46.0% (43.5-48.7%), respectively. The threshold values of ANI and GGD for species discrimination are 95-96% and 70%, respectively. These results reveal that strain ChDC B353 (= KCOM 1699 = JCM 33453) is a novel species belonging to genus Streptococcus, for which a name of Streptococcus chosunense sp. nov. is proposed.
Topics: Base Composition; Cysts; DNA, Bacterial; Fatty Acids; Genome, Bacterial; Humans; Male; Maxillary Diseases; Middle Aged; Phylogeny; RNA, Ribosomal, 16S; Republic of Korea; Sequence Analysis, DNA; Species Specificity; Streptococcus
PubMed: 31332483
DOI: 10.1007/s00284-019-01746-0 -
BMC Ophthalmology Nov 2021Parvimonas micra is known as a causative agent of chronic periodontal disease. This Gram-positive obligate anaerobic coccus was cultured from the ocular surface of...
BACKGROUND
Parvimonas micra is known as a causative agent of chronic periodontal disease. This Gram-positive obligate anaerobic coccus was cultured from the ocular surface of blowout fracture-related orbital cellulitis progressing to panophthalmitis.
CASE PRESENTATION
The patient was a woman in her fifties who had panic disorder and subsequently was a victim of domestic violence. These factors led to delayed consultation. At the initial visit to an ophthalmologist, the ocular surface of the right eye was covered with pus. Swelling of the upper and lower eyelids prevented the eyelid from closing and exophthalmos, severe corneal ulcer, panophthalmitis, and no light perception were observed. Head computed tomography revealed an old blowout fracture and chronic sinusitis with orbital cellulitis. P. micra were isolated from culture of pus samples from the sinus and from the ocular surface.
CONCLUSIONS
There is a possibility that P. micra invaded the orbit via the fragile bony site and caused orbital cellulitis, severe corneal ulcer, and panophthalmitis that required enucleation. In cases of coexisting old blowout fracture and chronic sinusitis, the chronic sinusitis should be treated as quickly as possible.
Topics: Female; Firmicutes; Humans; Orbit; Orbital Cellulitis; Orbital Fractures; Panophthalmitis
PubMed: 34758776
DOI: 10.1186/s12886-021-02153-5 -
BMC Infectious Diseases Apr 2021Parvimonas micra (P. micra) is a gram-positive anaerobic coccus that is detected widely on the skin, in the oral mucosa and in the gastrointestinal tract. In certain...
BACKGROUND
Parvimonas micra (P. micra) is a gram-positive anaerobic coccus that is detected widely on the skin, in the oral mucosa and in the gastrointestinal tract. In certain circumstances, P. micra can cause abdominal abscesses, bacteraemia and other infections. To the best of our knowledge, there have been no case reports describing the biological characteristics of P. micra-related pneumonia. These bacteria do not always multiply in an aerobic organ, such as the lung, and they could be easily overlooked because of the clinical mindset.
CASE PRESENTATION
A 35-year-old pregnant woman was admitted to the emergency department 4 weeks prior to her due date who was exhibiting 5 points on the Glasgow coma scale. A computed tomography (CT) scan showed a massive haemorrhage in her left basal ganglia. She underwent a caesarean section and brain surgery before being admitted to the ICU. She soon developed severe pneumonia and hypoxemia. Given that multiple sputum cultures were negative, the patient's bronchoalveolar lavage fluid was submitted for next-generation sequencing (NGS) to determine the pathogen responsible for the pneumonia; as a result, P. micra was determined to be the causative pathogen. Accordingly the antibiotic therapy was altered and the pneumonia improved.
CONCLUSION
In this case, we demonstrated severe pneumonia caused by the anaerobic organism P. micra, and the patient benefited from receiving the correct antibiotic. NGS was used as a method of quick diagnosis when sputum culture failed to distinguish the pathogen.
Topics: Adult; Anti-Bacterial Agents; Cesarean Section; Coma; Female; Firmicutes; Gram-Positive Bacterial Infections; Humans; Intracranial Hemorrhages; Pneumonia, Bacterial; Pregnancy; Pregnancy Complications, Infectious; Pregnancy Trimester, Third; Severity of Illness Index; Tomography, X-Ray Computed
PubMed: 33865326
DOI: 10.1186/s12879-021-06058-y -
Current Microbiology Nov 2019A novel Gram-stain-negative, motile, and facultative anaerobic coccus, strain ChDC F240 was isolated from human subgingival dental plaque of a gingivitis lesion. The...
A novel Gram-stain-negative, motile, and facultative anaerobic coccus, strain ChDC F240 was isolated from human subgingival dental plaque of a gingivitis lesion. The phylogenetic analysis based on the 16S ribosomal RNA gene (16S rDNA) sequence showed that the strain belonged to the genus Lautropia. 16S rDNA of strain ChDC F240 had the highest similarity to that of Lautropia mirabilis ATCC 51599 (98.8%). Major cellular fatty acids of strain ChDC F240 were C (43.9%) and C/C (38.1%). Draft genome of the strain was 3,834,139 bp in length and the G+C content was 65.0 mol%. Average nucleotide identity and genome-to-genome distance values between strain ChDC F240 and L. mirabilis ATCC 51599 were 81.99% and 28.50% (26.1-30.9%), respectively. These results reveal that strain ChDC F240 is a novel species within the genus Lautropia, for which the name Lautropia dentalis sp. nov. is proposed; type strain is ChDC F240 (= KCOM 2505 = JCM 33297).
Topics: Bacterial Typing Techniques; Base Composition; Burkholderiaceae; DNA, Bacterial; DNA, Ribosomal; Dental Plaque; Fatty Acids; Genome, Bacterial; Gingivitis; Humans; Phylogeny; RNA, Ribosomal, 16S
PubMed: 31446477
DOI: 10.1007/s00284-019-01761-1 -
BMC Infectious Diseases Jul 2022Campylobacter rectus is a gram-negative rod, and Parvimonas micra is a gram-positive coccus, both of which are oral anaerobes that cause chronic periodontitis. Chronic...
Femoral osteomyelitis caused by oral anaerobic bacteria with mixed bacteremia of Campylobacter rectus and Parvimonas micra in a chronic periodontitis patient: a case report.
BACKGROUND
Campylobacter rectus is a gram-negative rod, and Parvimonas micra is a gram-positive coccus, both of which are oral anaerobes that cause chronic periodontitis. Chronic periodontitis can cause bacteremia and systemic diseases, including osteomyelitis. Hematogenous osteomyelitis caused by anaerobic bacteria is uncommon, and to date, there have been no reports of mixed bacteremia with C. rectus and P. micra. Here, we report the first case of osteomyelitis of the femur caused by anaerobic bacteria with mixed bacteremia of C. rectus and P. micra caused by chronic periodontitis.
CASE PRESENTATION
A 75-year-old man with chronic periodontitis, hyperuricemia, and benign prostatic hyperplasia was admitted to the hospital with a fracture of the left femur. The patient had left thigh pain for 4 weeks prior to admission. Left femoral intramedullary nail fixation was performed, and a large amount of abscess and necrotic tissue was found intraoperatively. The cultures of abscess specimens were identified as P. micra, Fusobacterium nucleatum, and C. rectus. C. rectus and P. micra were also isolated from blood cultures. C. rectus was identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and 16 S ribosomal RNA sequencing. Sulbactam-ampicillin was administered for approximately 1 month, after which it was replaced by oral clavulanic acid-amoxicillin for long-term suppressive treatment.
CONCLUSIONS
Only five cases of bloodstream infection with C. rectus have been reported, and this is the first report of mixed bacteremia with P. micra. Clinicians should consider that chronic periodontitis caused by rare oral anaerobic bacteria can cause systemic infections, such as osteomyelitis.
Topics: Abscess; Aged; Bacteremia; Bacteria, Anaerobic; Campylobacter rectus; Chronic Periodontitis; Femur; Firmicutes; Humans; Male; Osteomyelitis; Peptostreptococcus
PubMed: 35836203
DOI: 10.1186/s12879-022-07573-2 -
Anaerobe Dec 2021Peptostreptococcus anaerobius is a gram-positive anaerobic coccus (GPAC) found in the gastrointestinal and vaginal microbiota. The organism is mainly found in... (Review)
Review
Peptostreptococcus anaerobius: Pathogenicity, identification, and antimicrobial susceptibility. Review of monobacterial infections and addition of a case of urinary tract infection directly identified from a urine sample by MALDI-TOF MS.
Peptostreptococcus anaerobius is a gram-positive anaerobic coccus (GPAC) found in the gastrointestinal and vaginal microbiota. The organism is mainly found in polymicrobial and scarcely in monobacterial infections such as prosthetic and native endocarditis. Anaerobic bacteria have rarely been reported as the cause of urinary tract infection (UTI). Although GPAC are susceptible to most antimicrobials used against anaerobic infections, P. anaerobius has shown to be more resistant. Herein, we report a case of UTI caused by P. anaerobius from a 62-year-old man with a history of urological disease. Surprisingly, the microorganism was directly identified by Matrix-Assisted Laser Desorption-Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) from the urine sample. The isolate was successfully identified by phenotypic methods, MALDI-TOF MS, and 16S rRNA gene sequencing. P. anaerobius showed no β-lactamase-producing activity, was resistant to penicillin, ampicillin, ciprofloxacin and levofloxacin, and displayed intermediate susceptibility to ampicillin-sulbactam and amoxicillin-clavulanic acid. Successful treatment was achieved with oral amoxicillin-clavulanic acid. Antimicrobial susceptibility testing (AST) should be performed on P. anaerobius isolates due to their unpredictable AST patterns and because empirically administered antimicrobial agents may not be active. This report shows that MALDI-TOF MS, directly used in urine specimens, may be a quick option to diagnose UTI caused by P. anaerobius or other anaerobic bacteria. This review is a compilation of monobacterial infections caused by P. anaerobius published in the literature, their pathogenicity, identification, and data about the antimicrobial susceptibility of P. anaerobius.
Topics: Anti-Infective Agents; Bacterial Typing Techniques; Disease Susceptibility; Gram-Positive Bacterial Infections; Humans; Male; Microbial Sensitivity Tests; Middle Aged; Molecular Typing; Peptostreptococcus; RNA, Ribosomal, 16S; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Treatment Outcome; Urinary Tract Infections
PubMed: 34626800
DOI: 10.1016/j.anaerobe.2021.102461