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International Journal of Systematic and... Feb 2020An obligately anaerobic, Gram-stain-positive and coccus-shaped bacterium, designated strain YH-panp20, was isolated from pig faeces. Phylogenetic analysis based on 16S...
An obligately anaerobic, Gram-stain-positive and coccus-shaped bacterium, designated strain YH-panp20, was isolated from pig faeces. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belongs to the family , and is most closely related to KCTC 15517 (93.5 % sequence similarity), followed by KCTC 5815 (92.2 %), KCTC 15521 (90.2 %) and KCTC 5969 (89.6 %). Average nucleotide identity values between YH-panp20 and its closest relatives were lower than 71 %. The G+C content of the isolate was 38.4 mol%, and its cell-wall peptidoglycan was found to be of A1γ type and contained -diaminopimelic acid. The predominant fatty acids were C 9, C DMA and C. The major end-products of glucose fermentation were lactate, acetate and formate. Therefore, based on the phenotypic, phylogenetic and chemotaxonomic properties, a novel genus and species, gen. nov., sp. nov., is proposed for isolate YH-panp20 (=KCTC 15747=JCM 32769).
Topics: Animals; Bacterial Typing Techniques; Base Composition; Cell Wall; DNA, Bacterial; Diaminopimelic Acid; Fatty Acids; Feces; Fermentation; Firmicutes; Gram-Positive Rods; Peptidoglycan; Phylogeny; RNA, Ribosomal, 16S; Republic of Korea; Sequence Analysis, DNA; Swine
PubMed: 31702538
DOI: 10.1099/ijsem.0.003803 -
BMJ Case Reports May 2020Necrotising fasciitis is a life-threatening condition characterised by inflammation, affecting the soft tissues, which spreads within a fascial plane. Skin changes can...
Necrotising fasciitis is a life-threatening condition characterised by inflammation, affecting the soft tissues, which spreads within a fascial plane. Skin changes can be delayed and can often go unnoticed. The condition arises from a bacterial infection, commonly being of polymicrobial aetiology. We describe an uncommon case of necrotising fasciitis caused by , an anaerobic coccus, in a 40-year-old patient with diabetes. is a Gram-positive anaerobic coccus, which was previously known as The bacteria is found in the normal flora of the urogenital tract. The bacteria is associated with severe infections such as native valve endocarditis, paravalvular abscess around a bioprosthetic valve, purulent pericarditis complicated by mediastanitis, meningitis after pneumonia and necrotising pneumonia complicated by pyopneumothorax. There have been no cases in the literature describing necrotising fasciitis of the abdominal wall caused by .
Topics: Abdominal Wall; Adult; Fasciitis, Necrotizing; Firmicutes; Humans; Male
PubMed: 32467123
DOI: 10.1136/bcr-2020-235115 -
Anaerobe Feb 2023Murdochiella asaccharolytica is a Gram-positive anaerobic coccus firstly isolated from wound specimens, since then, it was very rarely isolated from human infections. We...
Murdochiella asaccharolytica is a Gram-positive anaerobic coccus firstly isolated from wound specimens, since then, it was very rarely isolated from human infections. We report the first case of bloodstream infection due to this bacterium in a 90 years-old-man. The patient had a history of B-cell lymphoma and presented with pain and induration of the base of penis along with fever. A blood culture was obtained yielding M. asaccharolytica in pure culture. Only resistance to clindamycin was demonstrated. Treatment with metronidazole was started and the clinical evolution improved with antimicrobial treatment, being discharged after 6 days.
Topics: Male; Humans; Aged, 80 and over; Bacteria, Anaerobic; Bacterial Infections; Bacteremia; Metronidazole; Bacteria; Gram-Positive Cocci; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
PubMed: 36649886
DOI: 10.1016/j.anaerobe.2023.102697 -
Frontiers in Microbiology 2020is a major foodborne pathogen worldwide. As it forms biofilms, it can become a persistent contaminant in the food and pharmaceutical industries. In this study, it was...
is a major foodborne pathogen worldwide. As it forms biofilms, it can become a persistent contaminant in the food and pharmaceutical industries. In this study, it was demonstrated that could make more biofilm in aerobic conditions than in microaerobic conditions, and only 13.9% entered coccus (a VBNC state) under microaerobic conditions; however, the rate increased to 95.5% under aerobic conditions. could form more biofilm in mixed culture with or than in pure culture. Scanning electron microscope results showed that retained its normal spiral shape under aerobic conditions for 48 h by forming crosslinks with the aerobic and facultative anaerobic bacteria. Additionally, culture medium containing 0.5 mg/ml ZnO nanoparticles inhibited biofilm formation. Our results provide information on a new approach to controlling contamination via
PubMed: 32210924
DOI: 10.3389/fmicb.2020.00207 -
Infection and Drug Resistance 2022is a Gram-positive anaerobic coccus, which forms part of the normal flora and the human commensals of the skin, genitourinary system, and gut. It can cause...
is a Gram-positive anaerobic coccus, which forms part of the normal flora and the human commensals of the skin, genitourinary system, and gut. It can cause opportunistic infections in immunocompromised patients and is frequently isolated as part of polymicrobial spectra. Severe monomicrobial infections caused by the genus rarely occur. In this study, we report on septic shock, renal abscess, and bacteremia due to in a woman with nephrosis and diabetes mellitus. To the best of our knowledge, this report is the first to describe isolated from both renal abscess and blood cultures purely. The underlying diseases of the host and the removal of the double J tube were significant predisposing factors in this infection.
PubMed: 35281574
DOI: 10.2147/IDR.S353966 -
International Journal of Systematic and... Sep 2021A Gram-stain-negative, coccus-shaped, obligately anaerobic, non-motile and non-spore-forming bacterium, designated strain JN500902, was isolated from the mud in a...
A Gram-stain-negative, coccus-shaped, obligately anaerobic, non-motile and non-spore-forming bacterium, designated strain JN500902, was isolated from the mud in a fermentation cellar used continuously over 30 years for Chinese strong-flavour production. Colonies were white, circular, convex and smooth-edged. Growth was observed at 20-40 °C (optimum, 37 °C), at pH 5.0-10 (optimum, pH 7.5), with 0-2 % (w/v) NaCl and with 0-4 % (v/v) ethanol. The Biolog assay demonstrated positive reactions of strain JN500902 in the metabolism of l-fucose and pyruvate. The predominant cellular fatty acids (>10 %) consisted of C and C. The major end metabolites of strain JN500902 were acetic acid and ethanol when incubated anaerobically in liquid reinforced clostridial medium. Acetate was the major organic acid end product. The complete genome size of strain JN500902 was 3 420 321 bp with 3327 identified genes. The G+C content was 43.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain JN500902 with the family , having low sequence similarity (92.8 %) to the nearest type strain, DSM 3224 and forming a clearly distinct branch. Core genome phylogenetic analysis of the isolate and 134 strains belonging to the family also revealed that strain JN500902 was well-separated from other genera of this family as a monophyletic clade. The average nucleotide identity and amino acid identity values between strain JN500902 and 134 strains were less than 74 and 65 %, respectively. Considering its polyphasic characteristics, strain JN500902 represents a novel genus and species within the family , for which the name gen. nov., sp. nov. is proposed. The type strain is JN500902 (=CICC 24502=JCM 33939).
Topics: Anaerobiosis; Bacterial Typing Techniques; Base Composition; China; Clostridiales; DNA, Bacterial; Fatty Acids; Fermentation; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Soil Microbiology
PubMed: 34499596
DOI: 10.1099/ijsem.0.004991 -
IDCases 2023is an anaerobic, gram-positive coccus rarely identified as a pathogenic organism. However, case reports have suggested as a causative pathogen in bacteremia,...
is an anaerobic, gram-positive coccus rarely identified as a pathogenic organism. However, case reports have suggested as a causative pathogen in bacteremia, nosocomial pneumonia, ventilator-associated pneumonia, synovitis, cholecystitis, and ophthalmologic infections. Herein, we present the first case of causing native mitral valve infective endocarditis. With the exception of erythromycin, the isolate displayed favorable minimum inhibitory concentrations (MIC) to all other antibiotics tested, including beta-lactams, levofloxacin, and vancomycin. The patient was successfully treated with a 6-week course of intravenous (IV) ceftriaxone followed by robotically assisted cardiac valve repair.
PubMed: 37645537
DOI: 10.1016/j.idcr.2023.e01818 -
Archives of Microbiology Dec 2021Native Shewanella sp. RCRI7 is recently counted as an operative bacterium in the uranium bio-reduction. The aim of this study was to investigate the effects of uranium...
Native Shewanella sp. RCRI7 is recently counted as an operative bacterium in the uranium bio-reduction. The aim of this study was to investigate the effects of uranium tolerance on the morphology and population of RCRI7, following its potential removal capacity in different time intervals. In this research, the bacterial growth and uranium removal kinetic were evaluated in aerobic TSB medium, uranium-reducing condition (URC), aerobic uranium-containing (AUC) and anaerobic uranium-free (AUF) solution, following evaluations of omcAB gene expressions. In addition, spectrophotometry analyses were performed in URC confirming the bio-reduction mechanism. It was found that the bacteria can grow efficiently in the presence of 0.5 mM uranium anaerobically, unlike AUC and AUF solutions. Since the bacterium's adsorption capacity is quickly saturated, it can be deduced that uranium reduction should be dominant as incubation times proceed up to 84 h in URC. In 92 h incubation, the adsorbed uranium containing unreduced and reduced (U (IV) monomeric), was released to the solution due to either increased pH or bacterial death. In AUC and AUF, improper conditions lead to the reduced bacterial size (coccus-shape formation) and increased bacterial aggregations; however, membrane vesicles produced by the bacteria avoid the uranium incrustation in AUC. In overall, this study implies that Shewanella sp. RCRI7 are well tolerated by uranium under anaerobic conditions and the amount of regenerated uranium increases over time in the reduced form.
Topics: Adsorption; Biodegradation, Environmental; Kinetics; Oxidation-Reduction; Shewanella; Uranium
PubMed: 34958431
DOI: 10.1007/s00203-021-02716-6 -
Germs Dec 2021Emphysematous endocarditis is caused by the gas-forming organisms , , species, and . We report the first case of emphysematous endocarditis caused by .
INTRODUCTION
Emphysematous endocarditis is caused by the gas-forming organisms , , species, and . We report the first case of emphysematous endocarditis caused by .
CASE REPORT
An 82-year-old man presented with fever and rapidly progressive shortness of breath. He was found to be in atrial fibrillation with rapid ventricular rates. Two-dimensional transthoracic echocardiography demonstrated severe mitral regurgitation. Subsequent two- and three-dimensional transesophageal echocardiogram revealed a large, highly mobile vegetation on the atrial surface of the anterior mitral leaflet with aneurysmal destruction of the lateral scallop requiring mitral valve replacement. Sequencing of the vegetation revealed an anaerobic gram-positive coccus that, in rare cases, produces gas using a heme-dependent catalase. Histopathological analysis of the infected valve suggested interstitial gas accumulation, leading to the diagnosis of emphysematous endocarditis.
CONCLUSIONS
associated emphysematous endocarditis should be included in the differential diagnosis of valvular vegetation in patients with a rapidly progressing clinical course. When possible, histopathological analysis should be used alongside other imaging techniques to confirm the diagnosis of emphysematous endocarditis. This case also highlights the importance of collecting blood cultures prior to initiating antibiotic treatment.
PubMed: 35096679
DOI: 10.18683/germs.2021.1297 -
Respiratory Medicine Case Reports 2023is a gram-positive anaerobic coccus (GPAC) that colonizes the oral cavity and gastrointestinal tract. Recent advances in bacterial identification have confirmed the...
is a gram-positive anaerobic coccus (GPAC) that colonizes the oral cavity and gastrointestinal tract. Recent advances in bacterial identification have confirmed the clinical importance of . Here, we report a case of empyema with bacteremia caused by . We successfully treated the patient with the appropriate antibiotics and drainage. can cause respiratory infections, including empyema, which can progress to bacteremia if treatment is delayed. In infections, not only the oral cavity but also the entire body must be investigated to clarify the entry mechanism.
PubMed: 37577121
DOI: 10.1016/j.rmcr.2023.101892