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Archives of Microbiology Feb 2024Trueperella pyogenes (T. pyogenes) is an opportunistic pathogen that causes infertility, mastitis, and metritis in animals. T. pyogenes is also a zoonotic disease and is...
Trueperella pyogenes (T. pyogenes) is an opportunistic pathogen that causes infertility, mastitis, and metritis in animals. T. pyogenes is also a zoonotic disease and is considered an economic loss agent in the livestock industry. Therefore, vaccine development is necessary. Using an immunoinformatics approach, this study aimed to construct a multi-epitope vaccine against T. pyogenes. The collagen adhesion protein, fimbriae, and pyolysin (PLO) sequences were initially retrieved. The HTL, CTL, and B cell epitopes were predicted. The vaccine was designed by binding these epitopes with linkers. To increase vaccine immunogenicity, profilin was added to the N-terminal of the vaccine construct. The antigenic features and safety of the vaccine model were investigated. Docking, molecular dynamics simulation of the vaccine with immune receptors, and immunological simulation were used to evaluate the vaccine's efficacy. The vaccine's sequence was then optimized for cloning. The vaccine construct was designed based on 18 epitopes of T. pyogenes. The computational tools validated the vaccine as non-allergenic, non-toxic, hydrophilic, and stable at different temperatures with acceptable antigenic features. The vaccine model had good affinity and stability to bovine TLR2, 4, and 5 as well as stimulation of IgM, IgG, IL-2, IFN-γ, and Th1 responses. This vaccine also increased long-lived memory cells, dendritic cells, and macrophage population. In addition, codon optimization was done and cloned in the E. coli K12 expression vector (pET-28a). For the first time, this study introduced a novel multi-epitope vaccine candidate based on collagen adhesion protein, fimbriae, and PLO of T. pyogenes. It is expected this vaccine stimulates an effective immune response to prevent T. pyogenes infection.
Topics: Female; Animals; Cattle; Immunoinformatics; Escherichia coli; Vaccines; Epitopes, B-Lymphocyte; Collagen; Computational Biology; Bacterial Proteins; Bacterial Toxins; Hemolysin Proteins
PubMed: 38315222
DOI: 10.1007/s00203-023-03814-3 -
Microbiology Resource Announcements Jan 2024Many species of the genus are known as opportunistic pathogens and have been isolated in association with infectious diseases in humans and animals. Here, we present...
Many species of the genus are known as opportunistic pathogens and have been isolated in association with infectious diseases in humans and animals. Here, we present the complete genome sequence of another opportunistic pathogenic representative, namely , isolated from the otitis externa of an English bulldog.
PubMed: 38099684
DOI: 10.1128/mra.00624-23 -
International Journal of Molecular... Jun 2024is an important opportunistic pathogenic bacterium widely distributed in the environment. Pyolysin (PLO) is a primary virulence factor of and capable of lysing many...
is an important opportunistic pathogenic bacterium widely distributed in the environment. Pyolysin (PLO) is a primary virulence factor of and capable of lysing many different cells. PLO is a member of the cholesterol-dependent cytolysin (CDC) family of which the primary structure only presents a low level of homology with other members from 31% to 45%. By deeply studying PLO, we can understand the overall pathogenic mechanism of CDC family proteins. This study established a mouse muscle tissue model infected with recombinant PLO (rPLO) and its single-point mutations, rPLO N139K and rPLO F240A, and explored its mechanism of causing inflammatory damage. The inflammatory injury abilities of rPLO N139K and rPLO F240A are significantly reduced compared to rPLO. This study elaborated on the inflammatory mechanism of PLO by examining its unit point mutations in detail. Our data also provide a theoretical basis and practical significance for future research on toxins and bacteria.
Topics: Animals; Point Mutation; Mice; Hemolysin Proteins; NLR Family, Pyrin Domain-Containing 3 Protein; Bacterial Proteins; Inflammation; Potassium; Signal Transduction; Bacterial Toxins; Inflammasomes; Humans
PubMed: 38928408
DOI: 10.3390/ijms25126703 -
Microbiology Resource Announcements Sep 2020spp. are Gram-positive bacteria which can be found in a wide range of hosts and can be associated with disease in humans and animals. Here, we announce the complete...
spp. are Gram-positive bacteria which can be found in a wide range of hosts and can be associated with disease in humans and animals. Here, we announce the complete genome sequence of sp. strain 2701, isolated from a harbor seal from the North Sea.
PubMed: 32943560
DOI: 10.1128/MRA.00652-20 -
JMA Journal Sep 2019A 57-year-old man with untreated diabetes mellitus was admitted to our hospital due to an intrathoracic mass lesion infiltrating the vertebral body and mediastinum. The...
A 57-year-old man with untreated diabetes mellitus was admitted to our hospital due to an intrathoracic mass lesion infiltrating the vertebral body and mediastinum. The mass was suspected to be invasive lung cancer; however, percutaneous needle biopsy revealed that the mass was inflammatory granulation tissue caused by an infection. To the best of our knowledge, this is the first report of an intrathoracic mass lesion caused by an infection. When an intrathoracic mass lesion is suspected, clinicians should consider possible infections that cause granulation tissue, such as . This is particularly important in immunocompromized hosts such as patients with diabetes.
PubMed: 33615031
DOI: 10.31662/jmaj.2018-0036 -
New Microbes and New Infections Jun 2024
Expression of Concern: Arcanobacterium ihumii sp. nov., Varibaculum vaginae sp. nov. and Tessaracoccus timonensis sp. nov., isolated from vaginal swabs from healthy Senegalese women.
PubMed: 38799966
DOI: 10.1016/j.nmni.2024.101259 -
New Microbes and New Infections Sep 2019Culturomics studies the microbial variety of the human microbiome by combining diversified culture conditions, matrix-assisted laser desorption/ionization time-of-flight...
Culturomics studies the microbial variety of the human microbiome by combining diversified culture conditions, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and 16S rRNA gene identification. This study identifies three putative new bacterial species: sp. nov. strain Marseille-P5647, sp. nov. strain Marseille-P5644 and sp. nov. strain Marseille-P5995, which we describe according to the concept of taxonogenomics.
PubMed: 31367386
DOI: 10.1016/j.nmni.2019.100585 -
Microbiology Resource Announcements Jul 2021Bacteria of the genus can be found in a variety of hosts. The species Arcanobacterium phocisimile was originally identified in a free-living harbor seal in the German...
Bacteria of the genus can be found in a variety of hosts. The species Arcanobacterium phocisimile was originally identified in a free-living harbor seal in the German North Sea in 2004. Here, we announce the complete genome sequence of Arcanobacterium phocisimile strain DSM 26142.
PubMed: 34264110
DOI: 10.1128/MRA.00215-21 -
Radiology Case Reports Jul 2024, found as normal flora in healthy individuals, is an unusual culprit for pharyngitis and sinusitis in young adults, rarely leading to severe infections. Here, we...
, found as normal flora in healthy individuals, is an unusual culprit for pharyngitis and sinusitis in young adults, rarely leading to severe infections. Here, we present a singular case involving a 19-year-old immunocompetent male who experienced complications arising from sinusitis, leading to orbital and intracranial sinogenic complications. The patient developed severe cerebral vasospasm with delayed cerebral ischemia, necessitating aggressive management encompassing daily catheter-directed intra-arterial infusions, surgical source control, and maximal medical therapy. This case explores the challenging diagnostic and management aspects associated with cerebral artery vasospasm secondary to bacterial meningoencephalitis. The abrupt neurological decline in such patients presents a dilemma in recognizing the occurrence of cerebral vasospasm versus the progression of meningoencephalitis. By utilizing computed tomography brain perfusion scans, we were able to identify delayed cerebral ischemia due to cerebral vasospasm, acknowledging that this modality was not used to identify classical territorial stroke infarcts. This decision was made based on the understanding of the potential for bacterial-induced cerebral vasospasm to involve both hemispheres.
PubMed: 38645946
DOI: 10.1016/j.radcr.2024.03.029 -
New Microbes and New Infections Jun 2024
PubMed: 38799916
DOI: 10.1016/j.nmni.2024.101260