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Chemosphere Dec 2020A new treatment method using a deep eutectic solvent embedded melamine sponge (DES-MS) was studied for the removal of organic pollutants from water and soil samples....
A new treatment method using a deep eutectic solvent embedded melamine sponge (DES-MS) was studied for the removal of organic pollutants from water and soil samples. Five organophosphorus pesticides (OPPs) consisting of azinphos-methyl (AZP), parathion-methyl (PRT), fenitrothion (FNT), diazinon (DIZ) and chlorpyrifos (CPF), and two dyes including acid blue 29 (AB29) and malachite green (MG) were used as the model pollutants. DESs were easily prepared from tetrabutylammonium bromide (TBABr) and various fatty acids. The synthesised DESs were loaded into the sponge before being utilized for the removal of the studied pollutants. After the removal, the residual OPPs or dyes in the supernatant was quantified by high performance liquid chromatography or derivative spectrophotometry, respectively. The proposed method was simple, rapid, environmentally friendly and effective with the removal efficiency higher than 70% for various samples. Moreover, the removal of various dyes was successfully achieved with the efficiency greater than 65% under the optimum condition.
Topics: Coloring Agents; Environmental Restoration and Remediation; Limit of Detection; Pesticides; Resins, Synthetic; Soil; Soil Pollutants; Solvents; Triazines; Wastewater; Water Pollutants, Chemical; Water Purification
PubMed: 32679376
DOI: 10.1016/j.chemosphere.2020.127590 -
Water Research Jan 2024Assessing spatial variation in the chemical sensitivity of natural assemblages will enhance ecological relevance and reduce uncertainty in ecological risk assessments...
Assessing spatial variation in the chemical sensitivity of natural assemblages will enhance ecological relevance and reduce uncertainty in ecological risk assessments and the derivation of environmental quality standards (EQSs). However, the majority of species in natural communities have not undergone toxicity testing for any chemical, which poses a major challenge when assessing their sensitivity. We investigated spatial variation and patterns in the sensitivity of 4084 freshwater macroinvertebrate assemblages across England to 5 general-acting chemicals (heavy metals) and 13 specifically acting chemicals (insecticides) using a novel hierarchical species sensitivity distribution method based on taxonomic relatedness. Furthermore, we explored how river typology relates to spatial variation in assemblage sensitivity to chemicals and the potential impacts of such variation on current EQSs. Our findings revealed that, whereas assemblages with similar taxonomic compositions exhibit comparable sensitivity distributions, assemblages with different taxonomic compositions could have very similar or very different sensitivity distributions. The variation in assemblage sensitivity was greater for specifically acting chemicals than for general-acting chemicals and exhibited spatial clustering patterns. These spatial clustering patterns varied depending on the chemical, and the regions where assemblages were most sensitive to metals were generally not the same as the regions where assemblages were most sensitive to insecticides. Spatial variation in assemblage sensitivity was related to river typology with sensitive assemblages being more common than expected in lowland calcareous (or mixed geology) rivers within very small to small catchments. Comparing spatial variation in assemblage-specific chemical sensitivity to EQSs, we found that the operational EQSs in England would protect most study assemblages (i.e., > 99.5 %), although a small proportion of assemblages may face potential risks associated with azinphos-methyl, copper, and malathion. In many cases the EQSs were very precautionary, potentially requiring expensive control measures or restricting beneficial chemical use with no additional environmental benefit. The development of spatially defined EQSs, possibly based on river types, could be developed to target areas that require the highest level of protection and thus strike a balance between the benefits of chemical use and environmental protection.
Topics: Animals; Invertebrates; Insecticides; Fresh Water; Rivers; Conservation of Natural Resources; Ecosystem; Environmental Monitoring
PubMed: 37992635
DOI: 10.1016/j.watres.2023.120854 -
ALTEX 2022The photomotor response (PMR) of zebrafish embryos, a light pulse-triggered undirected movement, is known to be altered by neuroactive chemicals. Here, we developed an...
The photomotor response (PMR) of zebrafish embryos, a light pulse-triggered undirected movement, is known to be altered by neuroactive chemicals. Here, we developed an approach for data analysis of the distribution of PMR movement activities along the time axis; differences between treatment and respective controls are expressed by an aggregated value integrating the time-resolved density of the movement parameter as a measure for a chemically elicited PMR effect. Logistic concentration-PMR effect relationships were modeled for neuroactive test compounds with different modes of action (acetylcholinesterase inhibition, activation and inhibition of voltage-gated sodium channels); 50% effect concentrations (EC50) were in the low to medium μM range (EC50 < 10 μM for flucythrinate, esfenvalerate, azinphos-methyl, propoxur; EC50 > 10 μM for tricaine). Modulation of movement activities in different phases of the PMR (i.e., “fingerprint”) by neuroactive test compounds varied across concentrations, showing that mode of action-specific PMR fingerprints are also concentration-dependent. Above concentrations causing 10% lethality (LC10; 48 h), 3,4-dichloroaniline caused movement inhibition. This substance presumably is not neuroactive; its effect on the PMR therefore is considered a secondary toxic effect. Quantitative morphological examinations of chemically exposed embryos showed that malformations occurred only above PMR effect concentrations, indicating that changes in the PMR were not due to such indirect effects. The PMR assay will provide a useful measure in ecotoxicological risk assessment of neuroactive chemicals with zebrafish embryos and could potentially be used to infer acute fish toxicity levels from PMR effect concentrations of neurotoxic compounds.
Topics: Acetylcholinesterase; Animal Testing Alternatives; Animals; Data Analysis; Embryo, Nonmammalian; Neurotoxicity Syndromes; Zebrafish
PubMed: 34363684
DOI: 10.14573/altex.2004021 -
Frontiers in Bioengineering and... 2021In this study, was grown on municipal biosolids (BS) as the substrate to produce laccase for the removal of pesticides (fungicides, herbicides, and insecticides) from...
In this study, was grown on municipal biosolids (BS) as the substrate to produce laccase for the removal of pesticides (fungicides, herbicides, and insecticides) from wastewater. Among the various types of BS tested, sterilized biosolids were the most promising substrate for laccase production by with a maximal laccase activity (162.1 ± 21.1 U/g dry substrate), followed by hygenized biosolids (96.7 ± 17.6 U/g dry substrate), unsterilized biosolids (UBS) (31.9 ± 1.2 U/g dry substrate), and alkali-treated biosolids (8.2 ± 0.4 U/g dry substrate). The ultrasound-assisted extraction of this enzyme from fermented UBS was carried out with 0.1 M phosphate buffer at pH 7.0, which increased the enzyme activity of the crude extract by 30%. To test the catalytic potential of the biocatalyst in real matrices, 1 U/ml of recovered crude laccase extract was applied for 24 h for the removal of 29 pesticides (nine fungicides, 10 herbicides, and 10 insecticides) either separately or as a mixture from spiked biologically treated wastewater effluent. When treated with crude enzyme extract, high-priority herbicides metolachlor and atrazine were completely removed, while 93%-97% of the insecticides aldicarb, spinosad, and azinphos-methyl and up to 91% of kresoxim-methyl were removed. Promising results were obtained with BS-derived crude enzyme extract exhibiting improved pesticides removal, which may be due to the mediator effect resulting from the catalytic transformation of other molecules in the cocktail. The results demonstrated a promising integrated bioprocess for the removal of pesticides in wastewater using crude laccase obtained from BS.
PubMed: 35223809
DOI: 10.3389/fbioe.2021.770435 -
Ecotoxicology and Environmental Safety Jan 2024The insect cuticle consists of chitin and cuticular proteins (CPs), which stabilize the body shape and provide an effective physical barrier against the external...
The insect cuticle consists of chitin and cuticular proteins (CPs), which stabilize the body shape and provide an effective physical barrier against the external environment. They are also potential target sites for developing environmentally friendly insect management through the utilization of physiology-based methods. The codling moth, Cydia pomonella, is a pest afflicting fruit orchards worldwide. This study used a comparative genomic approach, whole-genome resequencing, and transcriptome data to understand the role that CPs played in the environmental adaptation of the codling moth. A total of 182 putative CPs were identified in the codling moth genome, which were classified into 12 CP families. 119 CPR genes, including 54 RR-1, 60 RR-2, and 5 RR-3 genes were identified and accounted for 65.4% of the total CPs. Eight and seven gene clusters are formed in RR1 and RR2 subfamily and the ancestor-descendant relationship was explained. Five CPAP genes were highly expressed during the egg stage and exposed to high temperature, which indicated their potential role in aiding codling moth eggs in acclimating to varying external heat conditions. Moreover, six CPs belonging to the CPR and CPLCP families were identified in association with insecticide resistance by population resequencing. Their expression levels increased after exposure to insecticides, suggesting they might be involved in codling moth resistance to the insecticides azinphos-methyl or deltamethrin. Our results provide insight into the evolution of codling moth CPs and their association with high temperature adaptation and insecticide resistance, and provide an additional information required for further analysis of CPs in environmental adaptation.
Topics: Humans; Animals; Moths; Insecticides; Temperature; Azinphosmethyl; Insecticide Resistance
PubMed: 38141334
DOI: 10.1016/j.ecoenv.2023.115852 -
Journal of Molecular Recognition : JMR Jul 2024Organophosphorus are typically hazardous chemicals used in the pharmaceutical, agricultural, and other industries. They pose a serious risk to human life and can be...
Organophosphorus are typically hazardous chemicals used in the pharmaceutical, agricultural, and other industries. They pose a serious risk to human life and can be fatal upon direct exposure. Hence, studying the interaction between such compounds with proteins is crucial for environmental, health, and food safety. In this study, we investigated the interaction mechanism between azinphos-methyl (AZM) and β-lactoglobulin (BLG) at pH 7.4 using a combination of biophysical techniques. Intrinsic fluorescence investigations revealed that BLG fluorescence was quenched in the presence of increasing AZM concentrations. The quenching mechanism was identified as static, as evidenced by a decrease in the fluorescence quenching constant (1.25 × 10, 1.18 × 10, and 0.86 × 10 M) with an increase in temperatures. Thermodynamic calculations (ΔH > 0; ΔS > 0) affirmed the formation of a complex between AZM and BLG through hydrophobic interactions. The BLG's secondary structure was found to be increased due to AZM interaction. Ultraviolet -visible spectroscopy data showed alterations in BLG conformation in the presence of AZM. Molecular docking highlighted the significant role of hydrophobic interactions involving residues such as Val43, Ile56, Ile71, Val92, Phe105, and Met107 in the binding between BLG and AZM. A docking energy of -6.9 kcal mol, and binding affinity of 1.15 × 10 M suggest spontaneous interaction between AZM and BLG with moderate to high affinity. These findings underscore the potential health risks associated with the entry of AZM into the food chain, emphasizing the need for further consideration of its impact on human health.
Topics: Lactoglobulins; Molecular Docking Simulation; Cattle; Animals; Thermodynamics; Azinphosmethyl; Pesticides; Spectrometry, Fluorescence; Hydrophobic and Hydrophilic Interactions; Protein Binding; Protein Structure, Secondary
PubMed: 38686702
DOI: 10.1002/jmr.3086 -
Ecology and Evolution Nov 2021Although insect herbivores are known to evolve resistance to insecticides through multiple genetic mechanisms, resistance in individual species has been assumed to...
Although insect herbivores are known to evolve resistance to insecticides through multiple genetic mechanisms, resistance in individual species has been assumed to follow the same mechanism. While both mutations in the target site insensitivity and increased amplification are known to contribute to insecticide resistance, little is known about the degree to which geographic populations of the same species differ at the target site in a response to insecticides. We tested structural (e.g., mutation profiles) and regulatory (e.g., the gene expression of and , AChE activity) differences between two populations (Vermont, USA and Belchow, Poland) of the Colorado potato beetle, in their resistance to two commonly used groups of insecticides, organophosphates, and carbamates. We established that Vermont beetles were more resistant to azinphos-methyl and carbaryl insecticides than Belchow beetles, despite a similar frequency of resistance-associated alleles (i.e., S291G) in the gene. However, the Vermont population had two additional amino acid replacements (G192S and F402Y) in the gene, which were absent in the Belchow population. Moreover, the Vermont population showed higher expression of and was less sensitive to AChE inhibition by azinphos-methyl oxon than the Belchow population. Therefore, the two populations have evolved different genetic mechanisms to adapt to organophosphate and carbamate insecticides.
PubMed: 34824806
DOI: 10.1002/ece3.8269 -
Environmental Toxicology and Chemistry Dec 2021We analyzed the reproductive success of eastern bluebirds (Sialia sialis) nesting in apple orchards and non-orchard nest-boxes in southern Ontario, Canada, from 1988 to...
We analyzed the reproductive success of eastern bluebirds (Sialia sialis) nesting in apple orchards and non-orchard nest-boxes in southern Ontario, Canada, from 1988 to 2018. Using data from 2397 nest-boxes monitored at 20 orchard sites and 52 non-orchard sites, we first modeled phenological parameters typically linked to climate change across both site types. We found that the first egg of each brood was laid significantly earlier in the season each year over our 31-year study. Clutch initiation occurred 4 days earlier in the spring in 2018 compared to 1988. Average clutch size in the first or second brood did not change significantly during our 31-year study; however, clutches were significantly smaller in orchards compared to non-orchards (0.10 ± 0.03 fewer eggs between sites). Nests built in orchards were also at 6.1-fold greater risk of parasitism and 2.1-fold greater risk of depredation than nests in non-orchards. After accounting for depredation and nest parasitism, hatching success was still significantly lower in orchards than in non-orchards. Overall, hatching success was 4%-5% lower in orchards. The probability of successfully fledging did not differ significantly between site types. In 2012, a ban on use of the organophosphate insecticide azinphos-methyl in orchards was enacted in Canada. We did not find a difference in hatching or fledging success in orchards after the ban. In our assessment of available data, we conclude that any pesticide effect on hatching success of eastern bluebirds in sprayed orchards is most likely the consequence of long-term exposure to dichlorodiphenyltrichloroethane (DDT) compounds in orchard soils and bioaccumulation in eggs rather than pesticides in use since regulation of DDT in the 1970s. Environ Toxicol Chem 2021;40:3369-3378. © 2021 Her Majesty the Queen in Right of Canada. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC. Reproduced with the permission of the Minister of Environment and Climate Change Canada.
Topics: Animals; Ecosystem; Female; Malus; Ontario; Pesticides; Songbirds
PubMed: 34551148
DOI: 10.1002/etc.5218 -
Chemosphere Mar 2023One of the key tenets of sustainable agriculture and food safety is the removal of toxic pesticides from the environment. However, developing reliable, affordable, and...
One of the key tenets of sustainable agriculture and food safety is the removal of toxic pesticides from the environment. However, developing reliable, affordable, and efficient methods for detecting and degrading pesticides into non-toxic degradable products remains an immediate matter of concern. Herein, we attempt to develop a strategy for the detection as well as degradation of highly toxic phosphorodithioate pesticide, Azinphos methyl (AZM), using hybrid zinc oxide nanoparticles (ZnO NPs). Considering the non-selectivity of bare ZnO and receptor R1, we have fabricated the heterocalixarene-based Calix (R1) over zinc oxide (ZnO) surface in situ via the sol-gel process. The synthesized heterocaliaxrene-modified ZnO (R1@ZnO) NPs show an excellent affinity for the selective and sensitive detection of AZM with a tremendously low limit of detection (68 mg L) and no interference from other pesticides. Degradation of AZM was fully supported by fluorescence spectroscopy, scanning electron microscopy (SEM), H NMR titrations, FTIR spectroscopy, cyclic voltammetry, and mass spectroscopy, which unequivocally confirmed the formation of non-toxic products. According to our findings, R1@ZnO NPs are sustainable nanomaterials that can be employed for environmental remediation since they operate in an aqueous medium.
Topics: Zinc Oxide; Azinphosmethyl; Nanoparticles; Microscopy, Electron, Scanning; Pesticides
PubMed: 36638927
DOI: 10.1016/j.chemosphere.2022.137693 -
RSC Advances Oct 2019A new bacterial genotoxicity detection strain was constructed, in which the cell lysis gene of from a lambda phage was controlled by a new designed SOS responsive...
A new bacterial genotoxicity detection strain was constructed, in which the cell lysis gene of from a lambda phage was controlled by a new designed SOS responsive element, designated as BL21/pUC-PST. The biosensor responded only after 0.5 h contact with mutagens and the changes in cell culture turbidity could be easily differentiated with the naked eyes from the control sample. This SOS/ system presented a dose-dependent manner to five model DNA-damaging agents with an improved detection sensitivity. The limits of detection (LODs) were 0.026 μM for mitomycin C, 320.4 μM for azinphos-methyl, 34.4 μM for methyl methanesulfonate, 4.6 μM for dithianone and 6.0 μM for dichlofluanid, which were much lower than previously reported. By performing binary and ternary mixture experiments, the toxic equivalency concept was validated in the SOS/ system by comparison with bioanalytical equivalent concentrations (BEQ) and overall toxic equivalent concentration (TEQ) using Cr(vi) as the reference compound. Pearson analysis indicated that a strong correlation existed between the TEQ and BEQ values. Thus the TEQ could be presented as the Cr(vi) equivalent concentration from its dose-effect lysis profiles for the environmental sample. The proposed genotoxicity reporter strain allows for easier qualitative characterization and quantitative interpretation of the TEQ values using Cr(vi) as the reference for environmental water samples.
PubMed: 35528065
DOI: 10.1039/c9ra06202e