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International Journal of Molecular... Dec 2021Decidualization is essential to the establishment of pregnancy in rodents and primates. Laminin A5 (encoding by ) is a member of the laminin family, which is mainly...
Decidualization is essential to the establishment of pregnancy in rodents and primates. Laminin A5 (encoding by ) is a member of the laminin family, which is mainly expressed in the basement membranes. Although laminins regulate cellular phenotype maintenance, adhesion, migration, growth, and differentiation, the expression, function, and regulation of laminin A5 during early pregnancy are still unknown. Therefore, we investigated the expression and role of laminin A5 during mouse and human decidualization. Laminin A5 is highly expressed in mouse decidua and artificially induced deciduoma. Laminin A5 is significantly increased under in vitro decidualization. Laminin A5 knockdown significantly inhibits the expression of , a marker for mouse decidualization. Progesterone stimulates the expression of laminin A5 in ovariectomized mouse uterus and cultured mouse stromal cells. We also show that progesterone regulates laminin A5 through the PKA-CREB-C/EBPβ pathway. Laminin A5 is also highly expressed in human pregnant decidua and cultured human endometrial stromal cells during in vitro decidualization. Laminin A5 knockdown by siRNA inhibits human in vitro decidualization. Collectively, our study reveals that laminin A5 may play a pivotal role during mouse and human decidualization via the PKA-CREB-C/EBPβ pathway.
Topics: Adult; Animals; CCAAT-Enhancer-Binding Protein-beta; Cyclic AMP; Cyclic AMP Response Element-Binding Protein; Cyclic AMP-Dependent Protein Kinases; Decidua; Female; Gene Expression Regulation, Developmental; Humans; Laminin; Male; Mice, Inbred ICR; Models, Biological; Pregnancy; Progesterone; Signal Transduction; Stromal Cells; Mice
PubMed: 35008625
DOI: 10.3390/ijms23010199 -
International Journal of Molecular... Dec 2022During decidualization in rodents, uterine stromal cells undergo extensive reprogramming to differentiate into distinct cell types, forming primary decidual zones...
During decidualization in rodents, uterine stromal cells undergo extensive reprogramming to differentiate into distinct cell types, forming primary decidual zones (PDZs), secondary decidual zones (SDZs), and layers of undifferentiated stromal cells. The formation of secondary decidual zones is accompanied by extensive angiogenesis. During early pregnancy, besides ovarian estrogen, de novo synthesis of estrogen in the uterus is essential for the progress of decidualization. However, the molecular mechanisms are not fully understood. Studies have shown that Cystatin B () is highly expressed in the decidual tissue of the uterus, but the regulation and mechanism of in the process of decidualization have not been reported. Our results showed that was highly expressed in mouse decidua and artificially induced deciduoma via in situ hybridization and immunofluorescence. Estrogen stimulates the expression of through the Estrogen receptor (ER)α. Moreover, in situ synthesis of estrogen in the uterus during decidualization regulates the expression of . Silencing the expression of affects the migration ability of stromal cells. Knockdown by siRNA significantly inhibits the expression of , a marker for mouse decidualization. Our study identifies a novel estrogen target, , during decidualization and reveals that may play a pivotal role in angiogenesis during mouse decidualization via the .
Topics: Pregnancy; Female; Mice; Animals; Decidua; Embryo Implantation; Estrogens; Uterus; Stromal Cells; Angiopoietin-Like Protein 7
PubMed: 36613747
DOI: 10.3390/ijms24010302 -
Reproductive Biology and Endocrinology... Oct 2021Decidualization is essential to the successful pregnancy in mice. The molecular mechanisms and effects of Aurora kinase A (Aurora A) remain poorly understood during...
BACKGROUND
Decidualization is essential to the successful pregnancy in mice. The molecular mechanisms and effects of Aurora kinase A (Aurora A) remain poorly understood during pregnancy. This study is the first to investigate the expression and role of Aurora A during mouse decidualization.
METHODS
Quantitative real time polymerase chain reaction, western blotting and in situ hybridization were used to determine the expression of Aurora A in mouse uteri. Aurora A activity was inhibited by Aurora A inhibitor to explore the role of Aurora A on decidualization via regulating the Aurora A/Stat3/Plk1/Cdk1 signaling pathway.
RESULTS
Aurora A was strongly expressed at implantation sites compared with inter-implantation sites. Furthermore, Aurora A was also significantly increased in oil-induced deciduoma compared with control. Both Aurora A mRNA and protein were significantly increased under in vitro decidualization. Under in vitro decidualization, Prl8a2, a marker of mouse decidualization, was significantly decreased by TC-S 7010, an Aurora A inhibitor. Additionally, Prl8a2 was reduced by Stat3 inhibitor, Plk1 inhibitor and Cdk1 inhibitor, respectively. Moreover, the protein levels of p-Stat3, p-Plk1 and p-Cdk1 were suppressed by TC-S 7010. The protein levels of p-Stat3, p-Plk1 and p-Cdk1 were also suppressed by S3I-201, a Stat3 inhibitor). SBE 13 HCl (Plk1 inhibitor) could reduce the protein levels of p-Plk1 and p-Cdk1. Collectively, Aurora A could regulate Stat3/Plk1/Cdk1 signaling pathway.
CONCLUSION
Our study shows that Aurora A is expressed in decidual cells and should be important for mouse decidualization. Aurora A/Stat3/Plk1/Cdk1 signaling pathway may be involved in mouse decidualization.
Topics: Animals; Aurora Kinase A; CDC2 Protein Kinase; Cell Cycle Proteins; Cells, Cultured; Decidua; Enzyme Inhibitors; Female; Mice; Pregnancy; Protein Serine-Threonine Kinases; Proto-Oncogene Proteins; STAT3 Transcription Factor; Signal Transduction; Polo-Like Kinase 1
PubMed: 34715887
DOI: 10.1186/s12958-021-00847-5 -
Chinese Herbal Medicines Jul 2020The objective of the present study was to evaluate the antifertility activity of ether (ErCD), chloroform (CeCD) and ethyl alcohol (EyCD) extracts of the whole plant of...
OBJECTIVE
The objective of the present study was to evaluate the antifertility activity of ether (ErCD), chloroform (CeCD) and ethyl alcohol (EyCD) extracts of the whole plant of in female Wistar albino rats.
METHODS
Acute oral toxicity and an antifertility study were performed in female Wistar rats with two dose levels (200 and 400 mg/kg, orally) of EyCD. The estrogenic and progestogenic effects of EyCD were further observed by administering it to immature Wistar rats by investigations of vaginal cornification, hormonal level, uterus weight, biochemical parameters, histopathology of the uterus and deciduoma formation, respectively. Isolation of EyCD was carried out by Flash Chromatography and isolated fraction was estimated by HPLC.
RESULTS
No toxicity with any of the extract was found up to the dose of 2000 mg/kg body weight. EyCD treated rats exhibited maximum reduction in pregnancy (83.33%). Estimation of EyCD on vaginal cornification, estrogen-induced uterotrophic assay and deciduoma model demonstrated vaginal cornification, significant ( < 0.01) increase in uterine weight and uterine proliferation in histopathology and reduced deciduoma formation respectively. Hormonal and biochemical parameters confirmed the above findings indicating estrogenic potential and antiprogestogenic potential of EyCD that might be attributed to the presence of phytoestrogen (apigenin) in EyCD.
CONCLUSION
The results suggested that extracts of possess significant antifertility activity, which is consistent with the literature reported in folk medicine of this plant in fertility regulation.
PubMed: 36119013
DOI: 10.1016/j.chmed.2020.06.001 -
AJP Reports Oct 2019Deciduosis is the presence of ectopic decidual tissue outside the uterus, pelvic, or abdominal organs usually associated with pregnancy. It usually presents as smaller...
Deciduosis is the presence of ectopic decidual tissue outside the uterus, pelvic, or abdominal organs usually associated with pregnancy. It usually presents as smaller lesions but can be larger vascular lesions. Typically, these masses are detected incidentally during operative procedures. Our patient was referred at 14 weeks for a large intrauterine mass detected on ultrasound examination that was initially thought to be an acardiac twin. The mass was highly vascularized. However, since the patient was asymptomatic, she strongly desired to continue the pregnancy. The pregnancy was followed closely from 14 to 39 weeks with serial ultrasound examinations. The vascularity was documented to diminish overtime and the mass appeared to convolute as well. Due to the decrease in vascularity of the mass, the patient was allowed spontaneous vaginal delivery at term. Following delivery of the fetus and the placenta, the mass was easily extracted manually without any complications.
PubMed: 31737406
DOI: 10.1055/s-0039-1697647 -
BMJ Case Reports Jul 2019We describe a case of a deciduoid mesothelioma, a rare variant of epithelioid mesothelioma, which is associated with a very poor prognosis. A review of the relevant...
We describe a case of a deciduoid mesothelioma, a rare variant of epithelioid mesothelioma, which is associated with a very poor prognosis. A review of the relevant literature is also included. The patient was a man with probable asbestos exposure and presented with classic features of pleural malignancy. Diagnosis was reached with close correlation between clinical, radiological and pathological findings.
Topics: Aged; Asbestos; Deciduoma; Environmental Exposure; Humans; Lung Neoplasms; Male; Mesothelioma; Mesothelioma, Malignant; Palliative Care; Pleural Neoplasms; Prognosis; Thoracoscopy; Tomography, X-Ray Computed
PubMed: 31289169
DOI: 10.1136/bcr-2019-229945