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Science (New York, N.Y.) Nov 2022Research raises standards for working with anthropological collections.
Research raises standards for working with anthropological collections.
Topics: Humans; Anthropology; Pneumonia; Racism; Dental Records; Dental Calculus; Social Justice
PubMed: 36378986
DOI: 10.1126/science.adf6049 -
Journal of International Society of... 2022The purpose of this study was to compare salivary and dental plaque (DP) composition between disabled children who require home care (DCHC) and a control group (CG) and...
AIMS
The purpose of this study was to compare salivary and dental plaque (DP) composition between disabled children who require home care (DCHC) and a control group (CG) and to correlate it with oral and systemic health factors.
MATERIALS AND METHODS
This cross-sectional study included 15 DCHC and 15 healthy children (aged between 4 and 10 years). The caregivers answered a questionnaire on disease diagnosis, medical history, dental history, and oral hygiene routine. In addition to clinical examination, saliva and DP samples were collected and analyzed using attenuated total reflection-Fourier transform infrared spectroscopy. Data were collected between January and December 2019. Student's and Kendall correlation tests were used.
RESULTS
Calculus (46.7%), bleeding on toothbrushing (53.3%), and gingival hyperplasia (40.0%) were prevalent in DCHC. The saliva of DCHC presented a higher amount of lipids and collagen and a lower amount of carbohydrates than that of the CG ( < 0.05). DP components were similar in DCHC and CG.
CONCLUSION
DCHC presented oral comorbidities and changes in salivary composition, compared with the CG.
PubMed: 35966916
DOI: 10.4103/jispcd.JISPCD_296_21 -
Journal of Veterinary Dentistry Sep 2019Periodontal disease (PD) in pregnant women has been correlated with negative impacts on pregnancy outcomes including low birth weight, preterm labor, and increased...
Periodontal disease (PD) in pregnant women has been correlated with negative impacts on pregnancy outcomes including low birth weight, preterm labor, and increased neonatal mortality. This study aimed to explore similar associations in dogs. Medical, dental, and reproductive records of healthy female beagles producing one or more litters within a research breeding facility were evaluated over a 1.5-year period. Dental scoring (1-6) was based on a facility-developed, subjective assessment of gingivitis and calculus deposition following parturition. Assessment of periodontitis was not performed. Statistical analyses were performed to find correlations between the dam's dental score and pregnancy outcomes (length of gestation, litter size, stillborn, and failure to thrive puppies). Dam age, litter size, and perinatal mortalities were controlled in the statistical models. Data were collected from 808 dams, 1344 litters, and 7197 puppies; 75.3% of the 1344 dam scores were categorized as dental score 3 or less with a mean of 2.7. None of the evaluated pregnancy outcomes had any significant correlations with the severity of dental scores. The lack of a significant relationship between dental score and pregnancy outcomes may be the result of the low number of dogs with severe gingivitis and calculus deposition, the variability of dental score timing postparturition, and/or the subjective facility-generated dental scoring method that did not include universally accepted methods for gingivitis and periodontitis assessment. Studies involving more animals with severe PD utilizing objective, universally accepted methods for gingivitis and periodontitis assessment are warranted to say with confidence no association exists.
Topics: Animals; Dogs; Female; Gingivitis; Periodontal Diseases; Periodontitis; Pregnancy
PubMed: 31928398
DOI: 10.1177/0898756419892202 -
Molecular Biology and Evolution Oct 2020Dental calculus, the calcified form of the mammalian oral microbial plaque biofilm, is a rich source of oral microbiome, host, and dietary biomolecules and is well...
Dental calculus, the calcified form of the mammalian oral microbial plaque biofilm, is a rich source of oral microbiome, host, and dietary biomolecules and is well preserved in museum and archaeological specimens. Despite its wide presence in mammals, to date, dental calculus has primarily been used to study primate microbiome evolution. We establish dental calculus as a valuable tool for the study of nonhuman host microbiome evolution, by using shotgun metagenomics to characterize the taxonomic and functional composition of the oral microbiome in species as diverse as gorillas, bears, and reindeer. We detect oral pathogens in individuals with evidence of oral disease, assemble near-complete bacterial genomes from historical specimens, characterize antibiotic resistance genes, reconstruct components of the host diet, and recover host genetic profiles. Our work demonstrates that metagenomic analyses of dental calculus can be performed on a diverse range of mammalian species, which will allow the study of oral microbiome and pathogen evolution from a comparative perspective. As dental calculus is readily preserved through time, it can also facilitate the quantification of the impact of anthropogenic changes on wildlife and the environment.
Topics: Animals; Biological Evolution; Dental Calculus; Diet; Drug Resistance, Microbial; Gorilla gorilla; Mammals; Metagenome; Microbiota; Mouth; Reindeer; Ursidae
PubMed: 32467975
DOI: 10.1093/molbev/msaa135 -
Journal of Proteome Research Oct 2023Dental calculus is becoming a crucial material in the study of past populations with increasing interest in its proteomic and genomic content. Here, we suggest further...
Dental calculus is becoming a crucial material in the study of past populations with increasing interest in its proteomic and genomic content. Here, we suggest further development of a protocol for analysis of ancient proteins and a combined approach for subsequent ancient DNA extraction. We tested the protocol on recent teeth, and the optimized protocol was applied to ancient tooth to limit the destruction of calculus as it is a precious and irreplaceable source of dietary, microbiological, and ecological information in the archeological context. Finally, the applicability of the protocol was demonstrated on samples of the ancient calculus.
PubMed: 37699853
DOI: 10.1021/acs.jproteome.3c00370 -
The Chinese Journal of Dental Research Sep 2023To investigate the dental caries status and periodontal status of disabled children and adolescents aged 0 to 18 years in China and provide suggestions for future...
OBJECTIVE
To investigate the dental caries status and periodontal status of disabled children and adolescents aged 0 to 18 years in China and provide suggestions for future policies.
METHODS
The cross-sectional survey included disabled children and adolescents aged 0 to 18 years in welfare institutions across 10 provinces in eastern, central and western regions in China, between November 2013 and May 2015. Oral health examination included dental caries status and periodontal status. The mean score for decayed, missing and filled teeth (dmft/ DMFT) due to caries in primary/permanent dentition, gingival bleeding rate and calculus rate were recorded and statistically evaluated. Data analysis was performed using SPSS 24.0 software (IBM, Armonk, NY, USA), and the level of statistical significance was P < 0.05.
RESULTS
The mean dmft/DMFT values of for groups aged 0 to 5, 6 to 12 and 13 to 18 years were 1.48 ± 3.00, 2.19 ± 2.94 and 1.78 ± 2.93, respectively. The mean scores for the groups aged 0 to 5 (P < 0.05) and 13 to 18 years (P < 0.01) showed a significant difference among different disability types. A significant difference between sexes was found only in the group aged 0 to 5 years (P < 0.05). Gingival bleeding rates were 13.70% for the group aged 0 to 5 years, 24.81% for the group aged 6 to 12 years and 42.06% for the group aged 13 to 18 years, and calculus rates were 5.48%, 22.41% and 47.62% for the three age groups, respectively. The gingival bleeding rate for all three age groups showed a significant difference between different disability types (P < 0.01), whereas calculus rates a showed significant difference only in the groups aged 6 to 12 and 13 to 18 years (P < 0.01).
CONCLUSION
The prevalence of dental caries, gingival bleeding and calculus in disabled children and adolescents in China is high. Disability type was strongly associated with oral health status. Specially designed oral health education and training are necessary for clinical professionals and caregivers.
Topics: Child; Humans; Adolescent; Disabled Children; Cross-Sectional Studies; Dental Caries; Oral Health; China; Gingival Hemorrhage
PubMed: 37732683
DOI: 10.3290/j.cjdr.b4330833 -
Journal of Dental Education Oct 2022The Central Regional Dental Testing Service in the United States administered its first manikin-based (M) dental hygiene licensing exam in 2020. The aim of this study...
OBJECTIVES
The Central Regional Dental Testing Service in the United States administered its first manikin-based (M) dental hygiene licensing exam in 2020. The aim of this study was to compare the licensure exam results of dental hygiene students examined using manikins versus live patients.
METHODS
After obtaining the ethical approval, the de-identified exam scores of three different cohorts (2019, 2020, and 2021) were collected from the academic record database of Carrington College, Dental Hygiene School, San Jose, California. The exam scores of the students were grouped based on the exam format conducted: either M or patient-based (P). Mann-Whitney U test and two-tailed Fisher's exact were used to compare the scores of the groups.
RESULTS
The scores of 108 dental hygiene graduates between 2019 and 2021 were analyzed. The study included 65 participants examined in group P and 43 participants in group M. There was no significant difference in the mean score between groups P and M (p = 0.46) or in the passing rate between the two groups (p = 0.52). However, a higher first-attempt passing rate was noted in the M group. Moreover, calculus removal scores were comparable between the two examination groups (p = 0.18).
CONCLUSION
The findings of this study supported the comparability of both manikin and live P exam outcomes. Therefore, the use of M exams may be considered for future examinations. Further studies are needed in other settings to confirm the efficacy of M exams in evaluating the students' clinical performance.
Topics: Dental Hygienists; Education, Dental; Educational Measurement; Humans; Licensure; Licensure, Dental; Manikins; Students; United States
PubMed: 35426129
DOI: 10.1002/jdd.12940 -
Australian Dental Journal Mar 2021This study investigated the possible correlations between patient-performed implant hygiene and peri-implant success and disease, as well as patient-reported outcomes,...
BACKGROUND
This study investigated the possible correlations between patient-performed implant hygiene and peri-implant success and disease, as well as patient-reported outcomes, in a community-based cohort.
METHODS
Fifty-one patients (78 implants) from two private general practices were surveyed on their dental implant treatment history, oral hygiene instructions (OHI) received, home hygiene habits and current implant concerns. Their dentition, plaque/calculus scores and clinical implant parameters were examined. Correlations between hygiene habits, risk factors, implant success and peri-implant disease rates were assessed.
RESULTS
Implants had a patient-reported mean time in function of 6.7 years. Floss (74.4%), interdental brushes (IDB) (44.9%) and mouthwash (39.7%) were commonly used, while 7.7% of implants were only cleaned by brushing. Over half (56.4%) of implants fulfilled the success criteria, 61.5% had peri-implant health, 24.4% had mucositis and 7.7% had peri-implantitis. Only brushing (P < 0.001) and detectable plaque/calculus (P < 0.001) were significantly associated with more peri-implant disease. Local prosthetic factors affecting cleaning accessibility significantly reduced implant success (P < 0.001). Patients reported mixed recall of implant OHI, 7.7% of implants were aesthetically unsatisfactory and 9.0% had peri-implant symptoms.
CONCLUSIONS
Lack of interproximal cleaning and the presence of plaque/calculus were significantly associated with peri-implant disease in a community-based general practice setting, and patients reported mixed recall of OHI.
Topics: Dental Implants; Humans; Oral Hygiene; Patient Reported Outcome Measures; Peri-Implantitis; Periodontal Index
PubMed: 33174206
DOI: 10.1111/adj.12806 -
Frontiers in Microbiology 2022is a reported calcifying bacterium that can usually be isolated from dental calculus and induce mineralization . In recent years, based on hybridization probe and... (Review)
Review
is a reported calcifying bacterium that can usually be isolated from dental calculus and induce mineralization . In recent years, based on hybridization probe and sequencing technology, researchers have discovered the central "pillar" role of in supragingival plaque, and many studies focused on bacterial interactions in the biofilm structure dominated by have been conducted. Besides, seems to be an indicator of "caries-free" oral status according to imaging and sequencing studies. Therefore, in this review, we summarize 's role in supragingival plaque based on the structure, interactions, and potential connections with oral diseases.
PubMed: 35875585
DOI: 10.3389/fmicb.2022.940643 -
Genes Feb 2021Recent advantages in paleomicrobiology have provided an opportunity to investigate the composition of ancient microbial ecologies. Here, using metagenome analysis, we...
Recent advantages in paleomicrobiology have provided an opportunity to investigate the composition of ancient microbial ecologies. Here, using metagenome analysis, we investigated the microbial profiles of historic dental calculus retrieved from archaeological human remains from postmedieval Latvia dated 16-17th century AD and examined the associations of oral taxa and microbial diversity with specific characteristics. We evaluated the preservation of human oral microbiome patterns in historic samples and compared the microbial composition of historic dental calculus, modern human dental plaque, modern human dental calculus samples and burial soil microbiota. Overall, the results showed that the majority of microbial DNA in historic dental calculus originated from the oral microbiome with little impact of the burial environment. Good preservation of ancient DNA in historical dental calculus samples has provided reliable insight into the composition of the oral microbiome of postmedieval Latvian individuals. The relative stability of the classifiable oral microbiome composition was observed. Significant differences between the microbiome profiles of dental calculus and dental plaque samples were identified, suggesting microbial adaptation to a specific human body environment.
Topics: Adolescent; Adult; Archaeology; Body Remains; Burial; Child; DNA, Ancient; DNA, Bacterial; Dental Calculus; Dental Plaque; Female; Humans; Latvia; Male; Metagenome; Microbiota; Middle Aged; Soil Microbiology; Young Adult
PubMed: 33671794
DOI: 10.3390/genes12020309