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Acta Odontologica Latinoamericana : AOL Aug 2023This study evaluated cytotoxicity and antioxidant gene expression of resin cements on human gingival fibroblasts (hGF).
AIM
This study evaluated cytotoxicity and antioxidant gene expression of resin cements on human gingival fibroblasts (hGF).
MATERIALS AND METHOD
RelyX Ultimate™(RXU), Variolink™II(VLII), and RelyXU200™(RXU200) resin cements were incubated with culture medium for 24 h to obtain eluates. Then, the eluates were applied over hGF to assess cell viability at 24 h, 48 h, and 72 h and antioxidant gene expression at 24 h. hGF cultures non-exposed to the eluates were used as Control. Data were submitted to ANOVA and Bonferroni tests (α≤0.05).
RESULTS
RXU and RXU200 reduced the number of viable cells in 24 h. Longer exposure to cement extracts caused cell death. Gene expression showed peroxiredoxin 1 (PRDX1) induction by all resin cement types, and superoxide dismutase 1 (SOD1) induction by RXU200 and VLII. Moreover, RXU200 induced not only PRDX1 and SOD1, but also glutathione peroxidase 1 (GPX1), catalase (CAT), and glutathione synthetase (GSS).
CONCLUSIONS
All resin cements showed toxicity, and induced antioxidant genes in hGF. Antioxidant gene induction is at least partly associated with cytotoxicity of tested cements to oxidative stress experience.
Topics: Humans; Resin Cements; Antioxidants; Superoxide Dismutase-1; Materials Testing; Dental Cements
PubMed: 37776509
DOI: 10.54589/aol.36/2/ -
Journal of Dental Research Aug 2021Ceramic restorations are often adhesively luted onto the tooth prep. The so-called touch-cure concept was developed to yield optimum polymerization of composite cement...
Ceramic restorations are often adhesively luted onto the tooth prep. The so-called touch-cure concept was developed to yield optimum polymerization of composite cement at the restoration-cement-tooth interface for immediate bond stabilization. Although this touch cure is theorized to initiate polymerization at the interface when the accelerator in the primer makes contact with the cement, this process has not yet been proven. This study aimed to elucidate the mechanism of touch cure by measuring the degree of conversion (DC) of composite cement applied with or without an accelerator-containing tooth primer (TP) versus an accelerator-free primer using real-time Fourier-transform infrared spectroscopy (RT-FTIR) and attenuated total reflection (ATR)-FTIR. Interfacial bond strength was measured in shear mode, the accelerator composition confirmed by X-ray fluorescence analysis (XRF), and the interfacial interaction of TP and composite cement with dentin investigated by X-ray diffraction (XRD), focused-ion-beam scanning electron microscopy (FIB-SEM) with 3-dimensional interface reconstruction, and transmission electron microscopy (TEM). RT/ATR-FTIR revealed the significantly highest DC when the composite cement was applied with the accelerator-containing primer. XRF disclosed a vanadium compound as a novel chemical accelerator within TP, instead of a classic chemical curing initiator system, to set off touch cure as soon the cement contacts the previously applied primer. Although the TP contains the acidic functional monomer 10-MDP for adhesion to tooth tissue, touch cure using the accelerator-containing TP combined the fastest/highest DC with the highest bond strength. FIB-SEM and TEM confirmed the tight interfacial interaction at dentin with submicron hybridization along with stable 10-MDP also Ca-salt nanolayering.
Topics: Composite Resins; Dental Bonding; Dentin; Dentin-Bonding Agents; Materials Testing; Methacrylates; Polymerization; Resin Cements; Surface Properties; Tensile Strength; Touch
PubMed: 33771050
DOI: 10.1177/00220345211001020 -
Dental Materials : Official Publication... Dec 2022To correlate trueness and cement-space characteristics of crowns milled chairside and in the laboratory with those of inkjet 3D-printed crowns, and to assess whether...
OBJECTIVES
To correlate trueness and cement-space characteristics of crowns milled chairside and in the laboratory with those of inkjet 3D-printed crowns, and to assess whether 3D-printing accuracy meets the clinical standard.
METHODS
Thirty crowns were either (1) milled using a chairside Cerec MCXL unit from Cerec Zirconia Mono L (Dentsply Sirona), (2) milled using a LX-O 5-axis (Matsuura Machinery) industrial machine from Initial Zirconia HT (GC), or (3) 3D-printed using an inkjet Carmel 1400 (Xjet) printer (n = 10). Crown trueness determined by comparing the original CAD with each visible-light digitized crown was correlated with the 3D cement-space characteristics recorded by micro-CT. Statistics involved Kruskal-Wallis testing and Spearman correlation.
RESULTS
Crown trueness at the intaglio marginal area positively correlated with the marginal and axial cement-space characteristics. 3D-printing revealed data in-between those of the two milling systems with undercut values being not statistically different from those recorded for chairside milling and a low overcut level that was statistically similar to that obtained by laboratory milling. Laboratory milling revealed a significantly better marginal accuracy with a consequently lower cement-space thickness. A higher overcut level was recorded for the chairside-milled crowns in the marginal/occlusal thirds, resulting in the significantly highest occlusal cement-space thickness and cement-volume percentage with a cement thickness above 120 µm (limit considered as clinically acceptable). No statistical difference in trueness was found for the external crown dimensions.
SIGNIFICANCE
The 3D-printed zirconia crowns provided sufficient manufacturing accuracy for clinical use. Accurate milling and printing of the crown's intaglio marginal area is primordial.
Topics: Dental Materials; Dental Cementum; Dental Cements; Printing, Three-Dimensional; Glass Ionomer Cements; Bone Cements; Crowns
PubMed: 36411148
DOI: 10.1016/j.dental.2022.11.002 -
British Dental Journal Nov 2021
Topics: Dental Bonding; Dental Cements; Resin Cements
PubMed: 34824406
DOI: 10.1038/s41415-021-3684-6 -
International Journal of Oral... Nov 2023To evaluate the survival of and incidence of mechanical complications with single- and multiple-unit cement-retained posterior implant-supported restorations with custom...
Survival and mechanical complications of single- and multiple-unit cement-retained posterior implant-supported restorations with custom CAD/CAM Atlantis titanium abutments: An up to 10-year retrospective analysis.
PURPOSE
To evaluate the survival of and incidence of mechanical complications with single- and multiple-unit cement-retained posterior implant-supported restorations with custom CAD/CAM Atlantis titanium abutments (Dentsply Sirona, Charlotte, NC, USA).
MATERIALS AND METHODS
This retrospective clinical study analysed 196 Astra Tech OsseoSpeed TX Internal Hexagon implants (Dentsply Sirona) placed in 85 patients between January 2011 and January 2021. Customised Atlantis titanium abutments and cement-retained metal-ceramic crowns were employed. The clinical outcomes recorded were implant and abutment survival rates, and mechanical complications. The results were analysed according to implant length and diameter, arch, implant position and single- or multiple-unit restoration.
RESULTS
Over the observation period (up to 10 years), implant and abutment survival rates were 98.5% and 100.0%, respectively. The mean observation period for the single- and multiple-unit implant-supported restorations was 106.00 ± 20.84 months, with a minimum of 41 months and a maximum of 120 months. For the 67 single-unit and 129 multiple-unit posterior implant-supported restorations, four mechanical complications were recorded: two cases of screw loosening, one case of chipping or fracture of veneering materials, and one case of crown decementation. No screw or abutment fractures were observed.
CONCLUSIONS
According to the results of this retrospective clinical study, cement-retained posterior implant-supported restorations with custom CAD/CAM Atlantis titanium abutments showed high survival rates over a follow-up period of up to 10 years. No statistically significant differences were recorded when comparing implant position, implant diameter, implant length, single- versus multiple-unit restoration and arch.
Topics: Humans; Titanium; Retrospective Studies; Dental Implants; Dental Cements; Glass Ionomer Cements
PubMed: 37994819
DOI: No ID Found -
BMC Oral Health Dec 2022This study aimed to evaluate the effect of virtual cement space and restorative materials on the fit of computer-aided design and computer-aided manufacturing (CAD-CAM)...
BACKGROUND
This study aimed to evaluate the effect of virtual cement space and restorative materials on the fit of computer-aided design and computer-aided manufacturing (CAD-CAM) endocrowns.
METHODS
A mandibular first molar tooth model received a butt joint margin endocrown preparation with a 2-mm occlusal thickness. Then, using a 3D-printing system, 120 copies of this prepared die were printed and assigned equally to three groups with different cement space settings (30, 60, and 120 μm) during the chairside CAD design. In the milling process, CAD-based models with a particular space setting were subdivided into four groups (n = 10) and fabricated from different CAD-CAM materials: Vita Suprinity (VS), Celtra Duo (CD), Lava Ultimate (LU), and Grandio blocs (GR). Finally, the endocrowns were stabilized over their corresponding models with siloxane and subjected to micro-computed tomography to measure the fit.
RESULTS
The cement space that was predesigned at 30 μm generated the largest marginal discrepancy (from 144.68 ± 22.43 μm to 174.36 ± 22.78 μm), which was significantly different from those at 60 μm and 120 μm (p < 0.001). The combination of VS or CD with a pre-setting cement space of 60 μm and the combination of LU or GR with a cement space of 120 μm showed better agreement between the predesigned and actual measured marginal gap widths. For internal adaptation, only the cement space set to 30 μm exceeded the clinically acceptable threshold (200 μm).
CONCLUSIONS
The setting of the cement space and restorative material significantly affected the marginal adaptation of CAD-CAM endocrown restorations. Considering the discrepancy between design and reality, different virtual cement spaces should be applied to ceramic and resin composite materials.
Topics: Humans; Dental Marginal Adaptation; Dental Porcelain; Crowns; Dental Prosthesis Design; X-Ray Microtomography; Materials Testing; Computer-Aided Design; Dental Materials; Ceramics; Dental Cements; Glass Ionomer Cements
PubMed: 36494663
DOI: 10.1186/s12903-022-02598-0 -
American Journal of Dentistry Oct 2023To evaluate the capacity of highly opaque cements on masking titanium abutment background.
PURPOSE
To evaluate the capacity of highly opaque cements on masking titanium abutment background.
METHODS
Dentin and titanium specimens were used to simulate respectively, a natural dental background and an implant abutment. To simulate the full-crowns, Y-TZP zirconia (ZC), lithium disilicate (LD), and resin composite (RC) blocks were used. The titanium specimens were divided into six cementation groups (n=10): two regular cements (BQM and RX); three opaque cements (MHA; VA and BHA); and a clear liquid (CL). The masking capacity of each cement was calculated as the color difference between the color of the crowns over dentin with clear liquid (reference) and the color of the crowns over the titanium with the different cements (ΔEab). Data were statistically analyzed using two-way ANOVA and Tukey test (α= 0.05).
RESULTS
Significant differences (P= 0.0001) were observed for both factors, cement and crown, and for the interactions between them. The results of Tukey test for cement were: BHA-2.25(0.98), MHA-2.94 (1.03), VA-3.45 (1.67), BQM-9.55 (2.77), RX-9.88 (3.12), CL-10.41 (3.47). The cements BHA, MHA and VA showed significantly smaller means than BQM, RX and CL. The results for crown were: ZC-3.66 (2.37), LD-7.50 (4.08), RC-8.08 (4.67). The means for all crown materials were significantly different. Highly opaque cements were more efficient on background masking than regular cements. Zirconia promoted the higher color masking while the resin composite the lowest.
CLINICAL SIGNIFICANCE
The use of a highly opaque cement can reduce the color interference of the titanium abutment background, favoring the esthetic outcome of metal-free cemented crowns.
Topics: Composite Resins; Crowns; Dental Abutments; Dental Cements; Dental Porcelain; Glass Ionomer Cements; Materials Testing; Resin Cements; Titanium; Zirconium
PubMed: 37865808
DOI: No ID Found -
Pediatric Dentistry Nov 2023to update the 2016 systematic review evidence for vital pulp therapy (VPT) for primary teeth affected by caries or trauma. The population, intervention, comparison,... (Meta-Analysis)
Meta-Analysis
to update the 2016 systematic review evidence for vital pulp therapy (VPT) for primary teeth affected by caries or trauma. The population, intervention, comparison, outcomes, and study (PICOS) design inclusion/exclusion was used for multiple databases. Risk of bias, meta-analyses using RevMan, and certainty of evidence was created. A total of 299 studies were included; no trauma was found. Indirect pulp treatment (IPT) resulted in 97 percent success. Two calcium silicate cement (CSC) pulpotomies' success using mineral trioxide aggregate (MTA) and Biodentine were 94 percent and 90 percent, respectively, greater than for direct pulp capping (DPC; 86 percent) and other pulpotomies (moderate certainty). The success of IPT versus pulpotomy at 24 months showed no significant difference (P=0.31). Different liners or capping agents did not affect the success of IPT (P=0.79) or DPC at 24 months (P=0.24). The two CSC pulpotomies were not significantly different based on 24-month success (P=0.34). The formocresol pulpotomy success at 24 months was significantly lower than for MTA (P=0.02). Ferric sulfate had a significant lower success at 24 months than MTA pulpotomy (69 percent versus 92 percent; P=0.03). Zinc oxide eugenol, as a singular pulpotomy, had low success (65 percent). Selective/stepwise caries removal did significantly better at avoiding pulp exposures than complete excavation (P<0.001). Complete, selective, and no caries removal (Hall technique [HT], steel crown placement with no caries removal) had no significant difference in pulp vitality success for deep caries at 24 months (P=0.29). For deep caries affecting vital incisors, pulpotomy had significantly greater success than pulpectomy (P=0.002). The following had no significant effect on MTA pulpotomy success: coronal pulp removal methods; irrigation solution; method to control hemorrhage; base over MTA; treatment in one or two visits; and anterior or posterior teeth. Vital pulp therapy success of indirect pulp treatment or two calcium silicate cement pulpotomies demonstrated improved success over direct pulp capping and other pulpotomies based on 24-month evidence with moderate certainty. The Hall technique did not significantly reduce pulp vitality success versus caries removal.
Topics: Humans; Calcium Compounds; Silicates; Dental Care; Dental Cements; Zinc Oxide-Eugenol Cement; Dental Pulp Capping; Pulpotomy; Glass Ionomer Cements; Tooth, Deciduous; Oxides; Drug Combinations; Treatment Outcome; Aluminum Compounds
PubMed: 38129755
DOI: No ID Found -
Journal of Prosthodontics : Official... Oct 2022To investigate the potential of adding silver-nanoparticle-containing amorphous calcium phosphate microparticles as bioactive fillers into commercially available...
PURPOSE
To investigate the potential of adding silver-nanoparticle-containing amorphous calcium phosphate microparticles as bioactive fillers into commercially available nonbioactive dental resin cement.
MATERIALS AND METHODS
Experimental cement was formulated by adding 7.5% silver-nanoparticle-containing amorphous calcium phosphate microparticles to Multilink Automix resin cement (Ivoclar Vivadent). The experimental cement was evaluated for shear bond strength (N = 11 per group) and demineralization/remineralization (N = 16 per group), with BioCem Universal BioActive cement (NuSmile) as the positive control and Multilink Automix cement as the negative control. One-way analysis of variance and post hoc tests were used to assess the significance of differences among or between the groups RESULTS: The addition of silver-nanoparticle-containing amorphous calcium phosphate microparticles at the level of 7.5% by weight into Multilink Automix did not have a statistically significant effect on the shear bond strength (p > 0.05), but statistically significantly increased the depth of remineralization on both dentin and enamel (p = 0.01 and p < 0.001, respectively) when compared to Multilink Automix alone. The experimental cement prepared in the present study was comparable to BioCem on the depths of remineralization on both dentin and enamel (p = 0.59 and p = 0.99, respectively).
CONCLUSION
When incorporated into nonbioactive commercial dental resin cements as bioactive fillers at the level of 7.5% by weight, silver-nanoparticle-containing amorphous calcium phosphate microparticles could provide remineralization potential without affecting the shear bond strength.
Topics: Resin Cements; Dental Cements; Silver; Materials Testing; Glass Ionomer Cements; Dental Materials; Shear Strength; Dental Bonding; Surface Properties; Dental Stress Analysis
PubMed: 34942682
DOI: 10.1111/jopr.13473 -
Journal of the Mechanical Behavior of... Jun 2023Cariogenic bacteria and dental plaque biofilm at prosthesis margins are considered a primary risk factor for failed restorations. Resin cement containing antibacterial...
Cariogenic bacteria and dental plaque biofilm at prosthesis margins are considered a primary risk factor for failed restorations. Resin cement containing antibacterial agents can be beneficial in controlling bacteria and biofilm. This work aimed to evaluate the impact of incorporating magnesium oxide nanoparticles (MgONPs) as an antibacterial filler into dual-cure resin cement on bacteriostatic activity and physical properties, including mechanical, bonding, and physicochemical properties, as well as performance when subjected to a 5000-times thermocycling regimen. Experimental resin cements containing MgONPs of different mass fractions (0, 2.5%, 5%, 7.5% and 10%) were developed. Results suggested that the inclusion of MgONPs markedly improved the materials' bacteriostatic effect against Streptococcus mutans without compromising the physical properties when its addition reached 7.5 wt%. The mechanical properties of the specimens did not significantly decline after undergoing aging treatment, except for the flexural properties. In addition, the cements displayed good bonding performance and the material itself was not prone to cohesive fracture in the failure mode analysis. Furthermore, MgONPs possibly have played a role in decelerating material aging during thermocycling and enhancing bonding fastness in the early stage of cementation, which requires further investigation. Overall, developing MgONPs-doped resin cements can be a promising strategy to improve the material's performance in inhibiting cariogenic bacteria at restoration margins, in order to achieve a reduction in biofilm-associated secondary caries and a prolonged restoration lifespan.
Topics: Resin Cements; Magnesium Oxide; Materials Testing; Anti-Bacterial Agents; Nanoparticles; Dental Cements; Dental Bonding
PubMed: 37068430
DOI: 10.1016/j.jmbbm.2023.105815