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Toxins Nov 2022Catalase (CAT) can eliminate oxygen radicals, but it is unclear whether exogenous CAT can protect chickens against deoxynivalenol (DON)-induced oxidative stress. This...
Catalase (CAT) can eliminate oxygen radicals, but it is unclear whether exogenous CAT can protect chickens against deoxynivalenol (DON)-induced oxidative stress. This study aimed to investigate the effects of supplemental CAT on antioxidant property and gut microbiota in DON-exposed broilers. A total of 144 one-day-old Lingnan yellow-feathered male broilers were randomly divided into three groups (six replicates/group): control, DON group, and DON + CAT (DONC) group. The control and DON group received a diet without and with DON contamination, respectively, while the DONC group received a DON-contaminated diet with 200 U/kg CAT added. Parameter analysis was performed on d 21. The results showed that DON-induced liver enlargement ( < 0.05) was blocked by CAT addition, which also normalized the increases ( < 0.05) in hepatic oxidative metabolites contents and caspase-9 expression. Additionally, CAT addition increased ( < 0.05) the jejunal CAT and GSH-Px activities coupled with T-AOC in DON-exposed broilers, as well as the normalized DON-induced reductions ( < 0.05) of jejunal villus height (VH) and its ratio for crypt depth. There was a difference ( < 0.05) in gut microbiota among groups. The DON group was enriched ( < 0.05) with some harmful bacteria (e.g., , , , , and /) that elicited negative correlations ( < 0.05) with jejunal CAT activity, and VH. DONC group was differentially enriched ( < 0.05) with certain beneficial bacteria (e.g., , , and ) that could benefit intestinal antioxidation and morphology. In conclusion, supplemental CAT alleviates DON-induced oxidative stress and intestinal damage in broilers, which can be associated with its ability to improve gut microbiota, aside from its direct oxygen radical-scavenging activity.
Topics: Animals; Male; Chickens; Catalase; Gastrointestinal Microbiome; Dysbiosis; Antioxidants; Oxidative Stress; Diet; Dietary Supplements; Animal Feed
PubMed: 36548727
DOI: 10.3390/toxins14120830 -
Tropical Animal Health and Production Jan 2022To investigate the potential of Ampelopsis grossedentata extract used as a feed additive, laying performance, egg quality, yolk cholesterol, plasma biochemical...
To investigate the potential of Ampelopsis grossedentata extract used as a feed additive, laying performance, egg quality, yolk cholesterol, plasma biochemical parameters, intestinal histology, and gut microbiota of hens (n = 60) were determined between basal diet (CK) and dietary supplementation with A. grossedentata extract (RT) for 11 weeks. The laying rate in RT group was 6.3 percentage points higher than in CK group together with feed conversion rate decreasing. Significant upregulation of immunoglobulin indexes and downregulation of lipid-related indexes in RT group were also found in comparison with CK group, suggesting that dietary supplementation with A. grossedentata extract benefited in immunity enhancing and blood-fat depressing. Meanwhile, the villus height in duodenum and villus height to crypt depth ratio in duodenum and jejunum of RT group were significantly higher than that of CK group, indicating that dietary supplementation with A. grossedentata extract facilitated nutrient adsorption via intestinal histology changing. Moreover, the richness, diversity, and composition of gut microbiota in RT group significantly altered with a comparison of CK group, including beneficial bacterium and pathogenic bacterium, revealing that dietary supplementation with A. grossedentata extract could modify gut microbiota communities to affect intestinal adsorption and pathogen invasion. In addition, the lipid metabolism-related insulin signing pathway was significantly enriched by gut microbiota in RT group, which were conducive to egg production elevation via facilitating blood lipid amelioration and insulin resistance alleviation. These results provided a basis for A. grossedentata extract served as a feed additive in the hen industry.
Topics: Ampelopsis; Animal Feed; Animals; Chickens; Diet; Dietary Supplements; Female; Plant Extracts
PubMed: 35015154
DOI: 10.1007/s11250-022-03044-7 -
Fish & Shellfish Immunology Apr 2023The present study aims to explore the effects of dietary fulvic acid (FA) supplementation on the growth performance, digestive enzyme activity and immune response of sea...
The present study aims to explore the effects of dietary fulvic acid (FA) supplementation on the growth performance, digestive enzyme activity and immune response of sea cucumber (Apostichopus japonicas). FA was used to replace 0 (control), 0.1, 0.5 and 1 g cellulose in the basic diet of sea cucumber to formulate four experimental feeds with equivalent nitrogen and energy denoted as F0, F0.1, F0.3 and F1, respectively. No significant differences were observed in the survival rate among all groups (P > 0.05). Results show that the body weight gain rate, specific growth rate, intestinal trypsin, amylase and lipase activities, serum superoxide dismutase, catalase, lysozyme, alkaline and acid phosphatase activities and disease resistance ability against the pathogen, Vibrio splendidus of the sea cucumbers fed with FA-containing diets were significantly higher than those of the control group (P < 0.05). The optimum dose of dietary FA supplementation required for the maximum growth of sea cucumber was 0.54 g/kg. Therefore, dietary FA supplementation to the feed of sea cucumber can significantly improve its growth performance immune response.
Topics: Animals; Sea Cucumbers; Dietary Supplements; Stichopus; Immunity, Innate; Animal Feed; Diet; Disease Resistance
PubMed: 36871631
DOI: 10.1016/j.fsi.2023.108662 -
Advances in Experimental Medicine and... 2022L-Arginine (Arg) plays a central role in the nitrogen metabolism (e.g., syntheses of protein, nitric oxide, polyamines, and creatine), blood flow, nutrient utilization,...
L-Arginine (Arg) plays a central role in the nitrogen metabolism (e.g., syntheses of protein, nitric oxide, polyamines, and creatine), blood flow, nutrient utilization, and health of ruminants. This amino acid is produced by ruminal bacteria and is also synthesized from L-glutamine, L-glutamate, and L-proline via the formation of L-citrulline (Cit) in the enterocytes of young and adult ruminants. In pre-weaning ruminants, most of the Cit formed de novo by the enterocytes is used locally for Arg production. In post-weaning ruminants, the small intestine-derived Cit is converted into Arg primarily in the kidneys and, to a lesser extent, in endothelial cells, macrophages, and other cell types. Under normal feeding conditions, Arg synthesis contributes 65% and 68% of total Arg requirements for nonpregnant and late pregnany ewes fed a diet with ~12% crude protein, respectively, whereas creatine production requires 40% and 36% of Arg utilized by nonpregnant and late pregnant ewes, respectively. Arg has not traditionally been considered a limiting nutrient in diets for post-weaning, gestating, or lactating ruminants because it has been assumed that these animals can synthesize sufficient Arg to meet their nutritional and physiological needs. This lack of a full understanding of Arg nutrition and metabolism has contributed to suboptimal efficiencies for milk production, reproductive performance, and growth in ruminants. There is now considerable evidence that dietary supplementation with rumen-protected Arg (e.g., 0.25-0.5% of dietary dry matter) can improve all these production indices without adverse effects on metabolism or health. Because extracellular Cit is not degraded by microbes in the rumen due to the lack of uptake, Cit can be used without any encapsulation as an effective dietary source for the synthesis of Arg in ruminants, including dairy and beef cows, as well as sheep and goats. Thus, an adequate amount of supplemental rumen-protected Arg or unencapsulated Cit is necessary to support maximum survival, growth, lactation, reproductive performance, and feed efficiency, as well as optimum health and well-being in all ruminants.
Topics: Animals; Arginine; Cattle; Citrulline; Diet; Dietary Supplements; Endothelial Cells; Female; Lactation; Milk; Pregnancy; Ruminants; Sheep
PubMed: 34807443
DOI: 10.1007/978-3-030-85686-1_10 -
Poultry Science Sep 2022In this study, the effects of 3 graded dietary levels (0.1%, 0.2%, and 0.4%) of naringin were studied in Three-Yellow breeder hens during the late laying period (55-62...
In this study, the effects of 3 graded dietary levels (0.1%, 0.2%, and 0.4%) of naringin were studied in Three-Yellow breeder hens during the late laying period (55-62 wk). A total of 480 Three-Yellow breeder hens (54-wk-old) were randomly divided into 4 groups (6 replicates of 20 hens): basal diet group (C), and basal diets supplemented with 0.1%, 0.2%, and 0.4% of naringin (N1, N2, and N3), respectively. Results showed that dietary supplementation with 0.1%, 0.2%, and 0.4% of naringin for 8 wk increased the laying rate and egg mass, enhanced egg yolk color, and decreased the feed egg ratio (P < 0.05). Meanwhile, compared with hens in C group, there were more preovulatory follicles and higher ovarian index as well as an enhanced ovarian somatic cell proliferation in hens of N2 and N3 groups (P < 0.05). With 0.2% and 0.4% naringin, glutathione concentration, the activity of catalase and total superoxide dismutase, and the total antioxidant capacity of ovarian tissues and serum increased (P < 0.05), while the contents of malondialdehyde and hydrogen peroxide decreased (P < 0.05). Moreover, compared to C group, the transcription levels of antioxidant genes in ovarian tissues increased in hens from N2 and N3 groups (P < 0.05). In conclusion, supplementation with 0.2% and 0.4% naringin both could improve the laying rate, ovarian and serum antioxidant capacity of Three-Yellow breeder hens during the late laying period.
Topics: Animal Feed; Animals; Antioxidants; Chickens; Diet; Dietary Supplements; Female; Flavanones
PubMed: 35901650
DOI: 10.1016/j.psj.2022.102023 -
Nutrients Nov 2023Cardiovascular disease (CVD) remains the first cause of mortality globally. Diet plays a fundamental role in cardiovascular health and is closely linked to the... (Review)
Review
Cardiovascular disease (CVD) remains the first cause of mortality globally. Diet plays a fundamental role in cardiovascular health and is closely linked to the development of CVD. Numerous human studies have provided evidence on the relationship between diet and CVD. By discussing the available findings on the dietary components that potentially influence CVD progression and prevention, this review attempted to provide the current state of evidence on healthy dietary choices for CVD. We focus on the effects of individual macronutrients, whole food products, and dietary patterns on the risks of CVD, and the data from population-based trials, observational studies, and meta-analyses are summarized. Unhealthy dietary habits, such as high intake of saturated fatty acids, sugar-sweetened beverages, red meat, and processed meat as well as high salt intake are associated with the increased risk of CVD. Conversely, increased consumption of plant-based components such as dietary fiber, nuts, fruits, and vegetables is shown to be effective in reducing CVD risk factors. The Mediterranean diet appears to be one of the most evidence-based dietary patterns beneficial for CVD prevention. However, there is still great debate regarding whether the supplementation of vitamins and minerals confers cardioprotective benefits. This review provides new insights into the role of dietary factors that are harmful or protective in CVD, which can be adopted for improved cardiovascular health.
Topics: Humans; Cardiovascular Diseases; Diet; Fruit; Diet, Mediterranean; Nutrients; Risk Factors
PubMed: 38068756
DOI: 10.3390/nu15234898 -
Journal of Animal Physiology and Animal... Mar 2022Weaning is one of the most stressful events in the pig's life, which disrupts physiological balance and leads to oxidative stress. It is reported that glucose oxidase...
Weaning is one of the most stressful events in the pig's life, which disrupts physiological balance and leads to oxidative stress. It is reported that glucose oxidase supplementation could alleviate oxidative stress in animals by increasing the concentration of antioxidant enzymes in vivo. The purpose of this study was to evaluate the effects of dietary supplementation of Aspergillus niger-expressed glucose oxidase (AN-GOX) on growth performance, nutrient digestibility, faecal microbiota, faecal gas emission and serum antioxidant enzyme parameters in weaning pigs. A total of 120 21-day-old weaning pigs [(Yorkshire ×Landrace) × Duroc] with an initial body weight of 6.54 ± 0.55 kg were used in a 21-day experiment (phase 1, days 1-7; phase 2, days 8-21) with a completely randomized block design. Pigs were randomly divided into 4 treatment groups with 6 replicate pens per treatment and 5 pigs per pen (2 barrows and 3 gilts). Dietary treatments were corn-soybean meal-based basal diet supplemented with 0, 0.01, 0.03 or 0.05% AN-GOX (1000 unit/g). The results of this study showed that average daily gain during days 1-7 and 1-21 and the concentrations of serum glutathione peroxidase and glutathione increased linearly at graduated doses of AN-GOX increased in the diet. However, dietary supplementation of AN-GOX had no effects on the apparent nutrient digestibility, faecal microbiota and faecal gas emission. In conclusion, supplementing AN-GOX to the diet of weaning pigs ameliorated weaning stress, which manifested as the increase in serum antioxidant enzyme levels, thus improving growth performance. The suitable dosage of AN-GOX used in the diet of weaning pigs was 0.05%.
Topics: Animal Feed; Animal Nutritional Physiological Phenomena; Animals; Aspergillus niger; Diet; Dietary Supplements; Digestion; Glucose Oxidase; Sus scrofa; Swine; Weaning
PubMed: 34075632
DOI: 10.1111/jpn.13576 -
Reproduction in Domestic Animals =... Nov 2022The physiological effects of dietary boron (B) supplementation for farm animals specifically goat on male fertility are still scarce and need deep investigation. Thus,...
The physiological effects of dietary boron (B) supplementation for farm animals specifically goat on male fertility are still scarce and need deep investigation. Thus, the current study was designed to investigate how adding B to the diet of male goats affected their testicular and thyroid activity. For that purpose, twelve male goats were divided randomly into two groups (six animals each); control group that was fed the basal diet and B group that was fed the basal diet containing 70 mg B/kg diet for 6 months. Serum samples were collected at different intervals, while testicular biopsies were obtained at the end of the experiment. The results showed that 6 months of dietary B supplementation resulted in a significant increment in serum B concentration. The results of repeated measure analysis showed that there were significant GROUP and TIME × GROUP interactions effects on blood testosterone levels (F = 119.408, p = .000 and F = 6.794, p = .013, respectively), demonstrating that compared with control, B supplementation caused a significant rise in serum testosterone levels over time. However, the mean animal body weights and the serum levels of triiodothyronine (T3) and thyroxine (T4) were kept comparable with the control ones at the different time points. The most striking finding is that B supplementation increased significantly the mRNA expression of the CYP17A1 which is essential for steroidogenesis (p < .001). In addition, a histological examination of testicular tissue corroborated our findings and demonstrated that B supplementation had a positive effect. As a result, B might be considered an excellent food supplement that could be safely added to the male goats' diet at the current dose to improve their reproductive capacity.
Topics: Animals; Male; Animal Feed; Boron; Diet; Dietary Supplements; Goats; Testosterone; Thyroid Gland
PubMed: 35864721
DOI: 10.1111/rda.14212 -
Animal Science Journal = Nihon Chikusan... 2023The current study was conducted to explore the effects of dietary Clostridium butyricum (C. butyricum) and fermented calcium (Ca) butyrate produced by C. butyricum...
The current study was conducted to explore the effects of dietary Clostridium butyricum (C. butyricum) and fermented calcium (Ca) butyrate produced by C. butyricum on the performance and egg quality of post-peak laying. A total of 384 50-week-old hens were fed a basal diet, the basal diet with 300 mg/kg of fermented Ca butyrate or 1 × 10 CFU/kg C. butyricum for 8 weeks. Hens received a C. butyricum exhibited higher yolk properties, albumen height, and Haugh unit. A diet with fermented Ca butyrate or C. butyricum increased the egg mass and the pre-grade yellow follicle number. RNA-sequencing (RNA-seq) data showed that these observations were associated with cytokine-cytokine receptor interaction and intestinal immune status. Accordingly, when compared with the basal diet group, Ca butyrate and C. butyricum addition decreased serum pro-inflammatory cytokine levels and increased the concentration of immunoglobulin A, along with improved intestinal barrier. In addition, dietary C. butyricum inclusion induced a higher abundance of Ruminococcaceae and Lachnospiraceae at the family level. In summary, dietary fermented Ca butyrate or C. butyricum supplementation improved egg quality and ovarian function, which might be related to the enhanced intestinal barrier and immunity in post-peak laying hens.
Topics: Animals; Female; Clostridium butyricum; Chickens; Diet; Calcium, Dietary; Dietary Supplements; Butyrates; Cytokines; Animal Feed
PubMed: 37818858
DOI: 10.1111/asj.13877 -
Poultry Science Mar 2021The objective of this study was to evaluate the effects of dietary Bacillus subtilis supplementation and calcium (Ca) levels on performance, eggshell quality, intestinal...
The objective of this study was to evaluate the effects of dietary Bacillus subtilis supplementation and calcium (Ca) levels on performance, eggshell quality, intestinal morphology, and relative calbindin-D28k (CALB1) mRNA level of laying hens in the late phase of production. An experiment employing a 2 × 3 factorial arrangement of 3 levels of Ca (3.5, 4.0, and 4.5%) and the absence or presence of B. subtilis was carried out with a total of 576 Hy-Line Brown laying hens aged 72 to 79 wk. Every group had 8 replicates of 12 birds each. The results showed that 4.0 and 4.5% Ca levels improved (P < 0.05) apparent retention and serum Ca content of aged laying hens. Compared with the 3.5% Ca level, the 4.0% Ca level in diets increased (P < 0.05) thickness, eggshell weight, shell ratio, and eggshell Ca content of aged laying hens. Moreover, breaking strength, thickness, eggshell weight, shell ratio, eggshell Ca content, apparent retention of Ca in g/day, apparent retention of Ca in percent, villus height, villus height/crypt depth, serum Ca level, and relative CALB1 mRNA level of aged laying hens were all increased (P < 0.05) by B. subtilis supplementation in diets. The supplemental B. subtilis decreased feed conversion ratio (P = 0.001) significantly. In addition, there was an interaction effect between increased Ca levels from 3.5 to 4.5% and B. subtilis supplementation on crypt depth in the duodenum (P < 0.05). In conclusion, we found that both the increase in dietary Ca level from 3.5 to 4.5% and B. subtilis supplementation could enhance intestinal Ca absorption and improve eggshell quality of laying hens in the late phase of production (72-79 wk of age). Dietary supplementation of B. subtilis accompanying the 4.0% Ca level was appropriate in enhancement of eggshell quality.
Topics: Animal Feed; Animals; Bacillus subtilis; Calcium; Chickens; Diet; Dietary Supplements; Egg Shell; Female; Probiotics
PubMed: 33518333
DOI: 10.1016/j.psj.2020.12.067