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Colloids and Surfaces. B, Biointerfaces Feb 2020Free polymer graphene aerogel nanoparticles (GA NPs) were synthesized by using reduction/aggregation of graphene oxide (GO) sheets in the presence of vitamin C (as a...
Free polymer graphene aerogel nanoparticles (GA NPs) were synthesized by using reduction/aggregation of graphene oxide (GO) sheets in the presence of vitamin C (as a biocompatible reductant agent) at a low temperature (40 °C), followed by an effective sonication. Synthesis of GA NPs in doxorubicin hydrochloride (DOX)-containing solution results in the simultaneous synthesis and drug loading with higher performance (than that of the separately synthesized and loaded samples). To investigate the mechanism of loading and the capability of GA NPs in the loading of other drug structures, two groups of ionized (DOX, Amikacin sulfate and, d-glucosamine hydrochloride) and non-ionized (Paclitaxel (PTX)) drugs were examined. Furthermore, the relationship between the bipolar level of DOX solution (contributing to H-bonding of DOX and GO) and the amount of DOX loading was investigated. The DOX showed higher loading (>3 times) than PTX, as anticancer drugs. Since both DOX and PTX possess aromatic structures, the higher loading of DOX was assigned to its positive partial charge and ionized nature. Accordingly, other drugs (having positive partial charge and ionized nature, but no aromatic structure) such as Amikacin sulfate and d-glucosamine hydrochloride presented higher loading than PTX. These results indicated that although the π-π interactions induced by aromatic structures are important in drug loading, the electrostatic interaction of ionized drugs with GO (especially through H-bonding) is the dominant mechanism. DOX-loaded GANPs showed high pH-sensitive release (equivalent to the carrier weight) after 5 days, which can indicate benefits in tumor cell acidic microenvironments in-vivo.
Topics: Amikacin; Antineoplastic Agents; Doxorubicin; Drug Liberation; Gels; Glucosamine; Graphite; Hydrogen-Ion Concentration; Nanoparticles; Paclitaxel; Particle Size; Surface Properties
PubMed: 31846894
DOI: 10.1016/j.colsurfb.2019.110712 -
Environmental Science and Pollution... May 2022The purpose of this investigation was to analyze the performance of magnetite graphene oxide modified with β-cyclodextrin (GO@FeO@β-CD) for adsorption of methotrexate...
The purpose of this investigation was to analyze the performance of magnetite graphene oxide modified with β-cyclodextrin (GO@FeO@β-CD) for adsorption of methotrexate (MTX) and doxorubicin (DOX) from aqueous solutions. Characterization of GO@FeO@β-CD was carried out using some methods. The perfect conditions for the adsorption of MTX and DOX were 7.0, 45 min, 20 mg, and 25 °C for solution pH, contact time, adsorbent dose, and temperature, respectively, with removal efficiency values of 97.8% and 98.5% for MTX and DOX, respectively. The adsorption kinetic of MTX and DOX via GO@FeO@β-CD followed pseudo second-order (PSO) model, while the adsorption isotherm obeyed Langmuir model by monolayer adsorption with maximum adsorption capacities of 198.5 and 204.5 mg g for MTX and DOX, respectively. Therefore, it could be argued that HCl and 0.1 mol L NaOH would reflect adequate elution properties for GO@FeO@β-CD recovery.
Topics: Adsorption; Doxorubicin; Ferrosoferric Oxide; Graphite; Hydrogen-Ion Concentration; Kinetics; Methotrexate; Water; Water Pollutants, Chemical; Water Purification; beta-Cyclodextrins
PubMed: 35044605
DOI: 10.1007/s11356-022-18725-x -
Biophysical Chemistry Sep 2022The presented study was designed to estimate the ability of hypericin to interact with the anticancer drug doxorubicin. The hetero-association of hypericin and...
The presented study was designed to estimate the ability of hypericin to interact with the anticancer drug doxorubicin. The hetero-association of hypericin and doxorubicin was investigated with absorption and fluorescence spectroscopy methods in aqueous solution of DMSO in two-component mixtures: doxorubicin-hypericin and three component mixtures: DNA-doxorubicin-hypericin. The data indicate that hypericin forms complexes with doxorubicin and that the association constants are on the order of 300,000 M in a buffer with 30% DMSO content. The absorption spectra of the hypericin - doxorubicin complexes were examined as well. Owing to its ability to interact with flat aromatic compounds, hypericin may potentially be used as an interceptor molecule to detoxification of patients after chemotherapy.
Topics: Anthracenes; Dimethyl Sulfoxide; Doxorubicin; Humans; Perylene; Spectrometry, Fluorescence
PubMed: 35905651
DOI: 10.1016/j.bpc.2022.106858 -
Macromolecular Bioscience Mar 2022As a stand-alone therapy strategy may not be sufficient for effective cancer treatment and a combination of chemotherapy with other therapies is a main trend in cancer...
As a stand-alone therapy strategy may not be sufficient for effective cancer treatment and a combination of chemotherapy with other therapies is a main trend in cancer treatment. A combination of chemotherapy and photothermal therapy (PTT) is reported here to achieve the goal of cascade multistage cancer treatment. A thermally responsive amphiphilic copolymer is designed and then a CuS nanoparticles (NPs)-based carbon monoxide (CO) photoinduced release system and doxorubicin (Dox) are encapsulated to construct the nanomedicine. The large-sized nanomedicine can accumulate in tumors after long circulation in vivo and will generate heat to act as a photothermal therapeutic agent by near infrared (NIR) light. Moreover, synergically release of CO and Dox is achieved and acted as a sensitized chemotherapeutic agent. The combination of PTT and chemotherapy sensitization can effectively eliminate active tumor cells in the periphery of the tumor. CuS NPs are also released after the degradation of nanomedicine and small-sized CuS NPs possess better tumor penetration and achieve penetration-enhanced PTT by further NIR irradiation, thereby effectively eliminating tumor cells inside solid tumors. Hence, cascade multistage cancer treatment of "combined PTT and chemotherapy sensitization"-"penetration-enhanced PTT" is achieved, and tumor cells are comprehensively and effectively eliminated.
Topics: Cell Line, Tumor; Doxorubicin; Humans; Nanoparticles; Neoplasms; Phototherapy; Photothermal Therapy; Polymers
PubMed: 34910842
DOI: 10.1002/mabi.202100429 -
Colloids and Surfaces. B, Biointerfaces Sep 2022Core-shell nanocomposites are one of the most important achievements in the fast-growing field of nanotechnology. The combination of multi-responsive nano-shell with...
Core-shell nanocomposites are one of the most important achievements in the fast-growing field of nanotechnology. The combination of multi-responsive nano-shell with luminescent and photothermal core has led to promising applications in various fields such as optics, electronics and medicine. In this work, a nanosized core-shell system composed by carbonized dots core and poly(N-isopropylacrylamide) shell was developed and the photothermal triggered release of doxorubicin was demonstrated. The system was fully characterized by H-NMR, DLS, Z-potential, AFM, optical absorption and fluorescence measurements. A photothermal conversion efficiency (η) value of about 67.9% and a doxorubicin photo-release rate value of about 1.0% min were measured. Molecular dynamic (MD) simulations data were in agreement with experimental results, at 310 K the coil-to-globule transition and a consequent desorption of doxorubicin from the polymer were observed. Both the radius of gyration and the fluctuation of the distance doxorubicin-PNIPAM pointed that the temperature above the LCST and the acid pH facilitated the polymer transition. Moreover, MD simulations and experimental data suggested an influence on the lower critical solution temperature (LCST) exerted by the number of polymer chains anchored to the carbon core.
Topics: Acrylic Resins; Doxorubicin; Drug Liberation; Polymers; Temperature
PubMed: 35716451
DOI: 10.1016/j.colsurfb.2022.112628 -
Cardiovascular Toxicology Aug 2023Doxorubicin is associated with cardiotoxicity, and physical exercise seeks to minimize the toxic effects of doxorubicin through physiological cardiac remodeling, as well...
Doxorubicin is associated with cardiotoxicity, and physical exercise seeks to minimize the toxic effects of doxorubicin through physiological cardiac remodeling, as well as the reduction of oxidative stress, evidenced by previous studies. This study aimed to analyze whether running training before treatment with doxorubicin influences tolerance to physical exertion and cardiotoxicity. Thirty-nine male Wistar rats, aged 90 days and weighing between 250 and 300 g, were divided into 4 groups: Control (C), Doxorubicin (D), Trained (T), and Trained + Doxorubicin (TD). Animals in groups T and DT were submitted to treadmill running for 3 weeks, 5 times a week at 18 m/min for 20-30 min before treatment with doxorubicin. Animals in groups D and DT received intraperitoneal injections of doxorubicin hydrochloride three times a week for two weeks, reaching a total cumulative dose of 7.50 mg/kg. Our results show an increase in total collagen fibers in the D group (p = 0.01), but no increase in the TD group, in addition to the attenuation of the number of cardiac mast cells in the animals in the TD group (p = 0.05). The animals in the TD group showed maintenance of tolerance to exertion compared to group D. Therefore, running training attenuated the cardiac damage caused by the treatment with doxorubicin, in addition to maintaining the tolerance to exertion in the rats.
Topics: Rats; Male; Animals; Cardiotoxicity; Antibiotics, Antineoplastic; Rats, Wistar; Physical Conditioning, Animal; Doxorubicin
PubMed: 37402033
DOI: 10.1007/s12012-023-09798-2 -
International Journal of Nanomedicine 2020The integration of NIR photothermal therapy and chemotherapy is considered as a promising technique for future cancer therapy. Hollow Prussian nanospheres have attracted...
BACKGROUND
The integration of NIR photothermal therapy and chemotherapy is considered as a promising technique for future cancer therapy. Hollow Prussian nanospheres have attracted much attention due to excellent near-infrared photothermal conversion effect and drug-loading capability within an empty cavity. However, to date, the hollow Prussian nanospheres have been prepared by a complex procedure or in organic media, and their shell thickness and size cannot be controlled. Thus, a simple and controllable route is highly desirable to synthesize hollow Prussian nanospheres with controllable parameters.
MATERIALS AND METHODS
Here, in our designed synthesis route, the traditional FeCl precursor was replaced with FeO nanospheres, and then the Prussian blue (PB) nanoparticles were engineered into hollow-structured PB (HPB) nanospheres through an interface reaction, where the FeO colloidal template provides Fe ions. The reaction mechanism and control factors of HPB nanospheres were systematically investigated. Both in vitro and in vivo biological effects of the as-synthesized HPB nanospheres were evaluated in detail.
RESULTS
Through systematical experiments, a solvent-mediated interface reaction mechanism was put forward, and the parameters of HPB nanospheres could be easily adjusted by growth time and template size under optimal water and ethanol ratio. The in vitro tests show the rapid and remarkable photothermal effects of the as-prepared HPB nanospheres under NIR laser irradiation (808 nm). Meanwhile, HPB nanospheres also demonstrated a high DOX loading capacity of 440 mg g as a drug carrier, and the release of the drug can be regulated by the heat from PB shell under the exposure of an NIR laser. The in vivo experiments confirmed the outstanding performance of HPB nanospheres in photothermal/chemo-synergistic therapy of cancer.
CONCLUSION
A solvent-mediated template route was developed to synthesize hollow Prussian blue (HPB) nanospheres in a simple and controllable way. The in vitro and in vivo results demonstrate the as-synthesized HPB nanospheres as a promising candidate due to their low toxicity and high efficiency for cancer therapy.
Topics: Combined Modality Therapy; Doxorubicin; Drug Carriers; Ferric Compounds; Ferrocyanides; Humans; Hyperthermia, Induced; Nanospheres; Phototherapy
PubMed: 32764943
DOI: 10.2147/IJN.S252505 -
Small (Weinheim An Der Bergstrasse,... Oct 2022Stimuli-responsive DNA hydrogels are promising candidates for cancer treatment, as they not only possess biocompatible and biodegradable 3D network structures as highly...
Stimuli-responsive DNA hydrogels are promising candidates for cancer treatment, as they not only possess biocompatible and biodegradable 3D network structures as highly efficient carriers for therapeutic agents but also are capable of undergoing programmable gel-to-solution transition upon external stimuli to achieve controlled delivery. Herein, a promising platform for highly efficient photothermal-chemo synergistic cancer therapy is established by integrating DNA hydrogels with Ti C T -based MXene as a photothermal agent and doxorubicin (DOX) as a loaded chemotherapeutic agent. Upon the irradiation of near-infrared light (NIR), temperature rise caused by photothermal MXene nanosheets triggers the reversible gel-to-solution transition of the DOX-loaded MXene-DNA hydrogel, during which the DNA duplex crosslinking structures unwind to release therapeutic agents for efficient localized cancer therapy. Removal of the NIR irradiation results in the re-formation of DNA duplex structures and the hydrogel matrix, and the recombination of free DOX and adaptive hydrogel transformations can also be achieved. As demonstrated by both in vitro and in vivo models, the MXene-DNA hydrogel system, with excellent biocompatibility and injectability, dynamically NIR-triggered drug delivery, and enhanced drug uptake under mild hyperthermia conditions, exhibits efficient localized cancer treatment with fewer side effects to the organisms.
Topics: DNA Adducts; Doxorubicin; Humans; Hydrogels; Neoplasms; Phototherapy
PubMed: 36056901
DOI: 10.1002/smll.202200263 -
Colloids and Surfaces. B, Biointerfaces Oct 2019In an ideal delivery system, carrier nanoparticles are used as a promising alternative with minimized adverse effects to treat a variety of diseases. The purpose of this...
In an ideal delivery system, carrier nanoparticles are used as a promising alternative with minimized adverse effects to treat a variety of diseases. The purpose of this study was to create a targeted delivery system for doxorubicin hydrochloride (DOX-HCl), using FeO-L@HSA-β-cyclodextrin (β-CD)/Allyl amine nanoparticles. In this study, magnetite nanoparticles (FeO) were produced by co-precipitation, while albumin nanoparticles (HSA) were produced by the desolvation method. The properties of the nanoparticles were studied by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and vibrating sample magnetometer (VSM). To evaluate the loading of the drug on the synthesized nanoparticles, ultraviolet-visible spectrophotometry (UV-Vis) and atomic force microscopy (AFM) were used. DOX-HCl loading was studied by two experiments: an optimization method (OM) and a one-factor-at-a-time method (OFATM). Response surface methodology (RSM) was utilized to optimize the parameters. The optimal conditions for drug loading of nanoparticles in OM and OFATM methods were 81.46% and 77%, respectively. The release of DOX-HCl drug from the synthesized nanoparticles at a temperature of 37 °C and specific time in pH 5.3 and pH 7.4 was 83.35% and 38.39%, respectively. To examine the cytotoxicity of nanoparticles with drugs, the MTT assay was performed using MCF-7 cancer cells. Finally, cell uptake was tested using inductively coupled plasma-mass spectrometry (ICP-MS).
Topics: Antibiotics, Antineoplastic; Cell Survival; Doxorubicin; Drug Carriers; Drug Liberation; Endocytosis; Factor Analysis, Statistical; Ferrosoferric Oxide; Humans; Kinetics; Lysine; MCF-7 Cells; Magnetite Nanoparticles; Serum Albumin, Human; beta-Cyclodextrins
PubMed: 31362156
DOI: 10.1016/j.colsurfb.2019.110368 -
International Journal of Molecular... Sep 2019We used a hydrogel-mediated dual drug delivery approach, based on an injectable glycol chitosan (GC) hydrogel, doxorubicin hydrochloride (DOX⋅HCl), and a complex of...
We used a hydrogel-mediated dual drug delivery approach, based on an injectable glycol chitosan (GC) hydrogel, doxorubicin hydrochloride (DOX⋅HCl), and a complex of beta-cyclodextrin (β-CD) and paclitaxel (PTX) (GDCP) for breast cancer therapy in vitro and in vivo. The hydrogel was swollen over 3 days and remained so thereafter. After an initial burst period of 7 hours, the two drugs were released in a sustained manner for 7 days. The in vitro cell viability test showed that GDCP had a better anticancer effect than well plate and DOX⋅HCl/PTX (DP). In addition, the in vivo tests, which evaluated the anticancer effect, systemic toxicity, and histology, proved the feasibility of GDCP as a clinical therapy for breast cancer.
Topics: Animals; Breast Neoplasms; Doxorubicin; Drug Delivery Systems; Female; Humans; Hydrogels; MCF-7 Cells; Mice; Mice, Nude; Paclitaxel; Xenograft Model Antitumor Assays
PubMed: 31547111
DOI: 10.3390/ijms20194671