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European Journal of Applied Physiology Oct 2023This study examined the thermoregulatory response and ergogenic effects of ice slurry (ICE) ingestion in hot environments with high and low relative humidity (RH).
PURPOSE
This study examined the thermoregulatory response and ergogenic effects of ice slurry (ICE) ingestion in hot environments with high and low relative humidity (RH).
METHODS
Eight males completed four trials in a crossover manner in dry (DRY: 34.7 ± 0.2 °C, 38 ± 2%RH) and humid heat (HUM: 34.8 °C ± 0.2 °C, 80 ± 1%RH). They ingested 8.0 g·kg of ICE (0.0 °C) or 37.5 °C water (CON) during 30 min before exercise, and three aliquots (3.2 g·kg) of ICE or CON during 45-min cycling at 50%[Formula: see text]O, followed by cycling to exhaustion at 80%[Formula: see text]O (TTE). Body core temperature (T), mean skin temperature (T), heart rate (HR), thermal comfort, thermal sensation and rating of perceived exertion (RPE) were measured.
RESULTS
Relative to CON, ICE improved TTE by 76.5 ± 96.5% in HUM and 21.3 ± 44.9% in DRY (p = 0.044). End-exercise T was lower in ICE versus CON in DRY (37.8 ± 0.4 °C versus 38.1 ± 0.3 °C, p = 0.005) and HUM (38.8 ± 0.4 °C versus 39.3 ± 0.6 °C, p = 0.004). ICE decreased HR, heat storage and heat strain index only in DRY (p < 0.001-0.018). ICE improved thermal sensation and comfort in DRY and HUM (p < 0.001-0.011), attenuated RPE in HUM (p = 0.012) but not in DRY (p = 0.065).
CONCLUSION
ICE tended to benefit performance in humid heat more than in dry heat. This is likely due to the reduced extent of hyperthermia in dry heat and the relative importance of sensory inputs in mediating exercise capacity.
Topics: Male; Humans; Hot Temperature; Body Temperature Regulation; Skin Temperature; Exercise; Eating; Body Temperature
PubMed: 37256293
DOI: 10.1007/s00421-023-05235-y -
International Journal of Circumpolar... Dec 2023A field study was conducted to examine the vulnerability of military divers to non-freezing cold injury (NFCI) during Arctic ice-diving operations. Participants were...
A field study was conducted to examine the vulnerability of military divers to non-freezing cold injury (NFCI) during Arctic ice-diving operations. Participants were instrumented with temperature sensors on the back of their hands and on the bottom of their big toe for each dive to measure cooling of their extremities. While NFCI was not diagnosed in any of the participants during this field study, the data indicate that the feet were particularly vulnerable during the dives given that they were mostly in a temperature zone that could cause pain and performance decrements. The data also show that for short term dives, the dry and wet suits with wet gloves in both configurations were thermally more comfortable for the hands than the dry suit with dry glove configuration; however, the latter would be more protective against potential NFCI during longer dives. Features such as hydrostatic pressure and repetitive diving that are unique to diving but not previously considered as risk factors for NFCI are examined herein and warrant deeper investigation given that symptoms of NFCI might be mistaken as decompression sickness.
Topics: Humans; Diving; Cold Temperature; Temperature; Arctic Regions; Extremities
PubMed: 36966493
DOI: 10.1080/22423982.2023.2190488 -
National Science Review Mar 2022Severe sandstorms reoccurred in the spring of 2021 after an absence for more than 10 years in North China. The dust source area, located in Mongolia, suffered...
Severe sandstorms reoccurred in the spring of 2021 after an absence for more than 10 years in North China. The dust source area, located in Mongolia, suffered destructive cooling and warming in early and late winter, which loosened the land. A lack of precipitation, excessive snow melt and strong evaporation resulted in dry soil and exiguous spring vegetation. A super-strong Mongolian cyclone developed on the bare and loose ground, and easily blew and transported large amounts of sand particles into North China. Furthermore, top-ranking anomalies (sea ice shift in the Barents and Kara Sea, and sea surface temperatures in the east Pacific and northwest Atlantic) were found to induce the aforementioned tremendous climate anomalies in the dust source area. Analyses, based on large-ensemble Coupled Model Intercomparison Project Phase 6, yield results identical to the reanalysis data. Thus, the climate variabilities at different latitudes and synoptic disturbances jointly facilitated the strongest spring sandstorm over the last decade.
PubMed: 35265339
DOI: 10.1093/nsr/nwab165 -
Klinicheskaia Laboratornaia Diagnostika Sep 2020Taking into account the impact of shipment method of biosamples is necessary for obtaining high-quality biological samples in biobanking and laboratory research. The...
Taking into account the impact of shipment method of biosamples is necessary for obtaining high-quality biological samples in biobanking and laboratory research. The impact of liquid nitrogen, dry ice and cold accumulators on the quality of biological markers was considered, as well as recommendations to reduce the impact of these methods of shipment. The liquid nitrogen provides the best preservation of samples, however, dry ice is used much more often during their transportation. When transporting certain types of cells using dry ice, there is the way to use CryoStor CS1 and Cell Banker 1 cryoprotectors. The dry ice has a significant effect on both the pH of liquid biological samples and the coagulological parameters of plasma samples. The penetration of CO2 into the sample leads to changes in the parameters of PTT and APPT, as well as to decrease the protein C and fibrinogen level under certain conditions. Serum and plasma samples exposed to dry ice for more than 16 hours should be thawed open at room temperature, or instead of it should be kept at -80 °C for 24 hours to avoid changes in coagulation parameters, The use of cold accumulators is unacceptable for long-term shipment of serum and plasma containing unstable biomarkers because of insufficiently low temperature (increase over time to -25 °C and above). Besides, metal pellets can be used as cold storage batteries at low temperatures (up to -80 ° C), but they are not as effective as dry ice, since it is able to hold the required temperature for much longer.
Topics: Biological Specimen Banks; Blood Coagulation Tests; Cryopreservation; Dry Ice; Humans; Temperature
PubMed: 33245651
DOI: 10.18821/0869-2084-2020-65-10-619-625 -
Neuropharmacology Nov 2022Chemotherapy-induced neuropathic pain (CINP) is a debilitating and difficult-to-treat side effect of chemotherapeutic drugs. CINP is marked with oxidative stress and...
Chemotherapy-induced neuropathic pain (CINP) is a debilitating and difficult-to-treat side effect of chemotherapeutic drugs. CINP is marked with oxidative stress and neuronal hypersensitivities. The peroxisome proliferator-activated receptor gamma (PPARγ) is a transcription factor that regulates genes involved in oxidative stress and inflammation. We hypothesize that PPARγ agonists are protective against CIPN by reducing oxidative stress and inhibiting neuronal hypersensitivities. To test our hypothesis, acute or chronic CIPN was introduced by short or long-term treatment of oxaliplatin in BALB/c mice. CIPN mice were treated with either a novel blood-brain barrier (BBB) penetrable PPARγ agonist ELB00824, or a BBB non-penetrable PPARγ agonist pioglitazone, or vehicle. Cold allodynia, mechanical allodynia, motor coordination, sedation and addiction were measured with dry ice, von Frey filaments, beam-walking tests, and conditioned place preference, respectively. Oxidative stress was accessed by measuring byproducts of protein oxidation (carbonyl and 3-Nitrotyrosine) and lipid peroxidation [Thiobarbituric acid reactive substances (TBARS)], as wells as gene expression of Cat, Sod2, Ppargc1a. The effects of ELB00824 on nociceptor excitability were measured using whole-cell electrophysiology of isolated dorsal root ganglion neurons. Preemptive ELB00824, but not pioglitazone, reduced oxaliplatin-induced cold and mechanical allodynia and oxidative stress. ELB0824 suppressed oxaliplatin-induced firing in IB4 neurons. ELB00824 did not cause motor discoordination or sedation/addiction or reduce the antineoplastic activity of oxaliplatin (measured with an MTS-based cell proliferation assay) in a human colon cancer cell line (HCT116) and a human oral cancer cell line (HSC-3). Our results demonstrated that ELB00824 prevents oxaliplatin-induced pain, likely via inhibiting neuronal hypersensitivities and oxidative stress.
Topics: Animals; Antineoplastic Agents; Humans; Hyperalgesia; Hypersensitivity; Mice; Neuralgia; Neurons; Oxaliplatin; Oxidative Stress; PPAR gamma
PubMed: 36007855
DOI: 10.1016/j.neuropharm.2022.109233 -
Cryobiology Dec 2023In order to make dry ice transportation of vitrified embryos practical, a near-equilibrium vitrification was developed using a cryoprotectant solution (EDFS10/10a), by...
In order to make dry ice transportation of vitrified embryos practical, a near-equilibrium vitrification was developed using a cryoprotectant solution (EDFS10/10a), by which mouse embryos at various stages were vitrified in a near-equilibrium environment. EDFS10/10a consisted of 10% (v/v) ethylene glycol, 10% (v/v) MeSO, 0.4 M sucrose and 24% (w/v) Ficoll PM70. This method exhibited the benefits of slow freezing and vitrification, with a low risk of osmotic injury. In this study, we investigated whether mouse oocytes are vitrifiable with EDFS10/10a in a highly dehydrated/concentrated state, and whether they can remain fertilizable and developing into embryos after vitrification. When mature mouse oocytes were vitrified in liquid nitrogen and after 4-28 days of storage at -80 °C, high survival rates were observed (88-99%). Vitrified and warmed oocytes were subjected to partial zona dissection and in vitro fertilized. The rate of 2-cell stage was 80-82%. Blastocyst formation rate was 55-70% which was similar to that of embryos derived from fresh oocytes. After the 2-cell embryos were transferred to recipient mice, the implantation and offspring rates did not differ significantly from those of embryos derived from fresh oocytes, indicating that vitrified oocytes retained the developmental ability. Therefore, it is possible to vitrify mouse oocytes in a near-equilibrium state using EDFS10/10a and conveniently transported using dry ice.
Topics: Mice; Animals; Vitrification; Cryopreservation; Dry Ice; Cryoprotective Agents; Oocytes; Blastocyst
PubMed: 37722470
DOI: 10.1016/j.cryobiol.2023.104586 -
Journal of Cell Science Nov 2019This paper describes a simple, hazard-free and inexpensive procedure that allows researchers to send cultured cells across the globe at ambient temperatures. The method...
This paper describes a simple, hazard-free and inexpensive procedure that allows researchers to send cultured cells across the globe at ambient temperatures. The method enables transit of up to 2 weeks without compromising cell recovery. Its use will assist collaborators in distant laboratories to exchange cells without using dry-ice.
Topics: Animals; Cell Culture Techniques; Cell Survival; Dry Ice; Ice; Laboratories; Rats; Time Factors
PubMed: 31578238
DOI: 10.1242/jcs.238139 -
Cryobiology Mar 2024Cryopreserved semen is routinely shipped in liquid nitrogen. Dry ice could serve as an alternative coolant, however, frozen storage above liquid nitrogen temperatures...
Cryopreserved semen is routinely shipped in liquid nitrogen. Dry ice could serve as an alternative coolant, however, frozen storage above liquid nitrogen temperatures (LN2, -196 °C) may negatively affect shelf-life and cryosurvival. In this study, we determined critical temperatures for storage of cryopreserved stallion sperm. We evaluated: (i) effects of cooling samples to different subzero temperatures (-10 °C to -80 °C) prior to storing in LN2, (ii) stability at different storage temperatures (i.e., in LN2, dry ice, -80 °C and -20 °C freezers, 5 °C refrigerator), and (iii) sperm cryosurvival during storage on dry ice (i.e., when kept below -70 °C and during warming). Furthermore, (iv) we analyzed if addition of synthetic polymers (PVP-40, Ficoll-70) modulates ice crystallization kinetics and improves stability of cryopreserved specimens. Sperm motility and membrane intactness were taken as measures of cryosurvival, and an artificial insemination trial was performed to confirm fertilizing capacity. We found that adding PVP-40 or Ficoll-70 to formulations containing glycerol reduced ice crystal sizes and growth during annealing. Post-thaw sperm viability data indicated that samples need to be cooled below -40 °C before they can be safely plunged and stored in LN2. No negative effects of relocating specimens from dry ice to LN2 and vice versa became apparent. However, sample warming above -50 °C during transport in dry ice should be avoided to ensure preservation of viability and fertility. Moreover, addition of PVP-40 or Ficoll-70 was found to increase sperm cryosurvival, especially under non-ideal storage conditions where ice recrystallization may occur.
Topics: Male; Animals; Horses; Cryopreservation; Semen; Dry Ice; Ice; Polymers; Crystallization; Ficoll; Semen Preservation; Sperm Motility; Spermatozoa; Nitrogen; Povidone
PubMed: 38295927
DOI: 10.1016/j.cryobiol.2024.104852 -
Materials (Basel, Switzerland) Aug 2022This article presents the results of a numerical experimental study on the simulation of the dry ice compaction process. The first part of the article presents a...
This article presents the results of a numerical experimental study on the simulation of the dry ice compaction process. The first part of the article presents a description of the material used, material models and the methodology of experimental research. In the second part, numerical and experimental study results are presented. For the purpose of comparison, a parametric method based on the residual sum of squares was used. The application of the indicated method fills the gap in the available literature as the authors are not aware of any existing data from previous studies on the method of comparing the results of numerical tests in terms of the obtained results and the change of the value of the tested parameter as a function of another variable. The results of this study can be useful in research work aimed at further development of the process of extrusion and compaction of dry ice using Drucker-Prager/Cap and modified Cam-Clay material models for instance for optimization of geometric parameters of parts and components of the main assembly of the machine used in the process of dry ice extrusion.
PubMed: 36013907
DOI: 10.3390/ma15165771 -
Journal of Fish Biology Mar 2020Larval fishes provide a valuable metric for assessing and monitoring species, populations, and ecosystem trends and condition. However, taxonomic resolution for this...
Larval fishes provide a valuable metric for assessing and monitoring species, populations, and ecosystem trends and condition. However, taxonomic resolution for this life stage is inherently problematic because of their individual sizes, limited morphological characteristics and high tissue degradation rates. There is little research on methods that rapidly preserve larval tissues for later morphological and molecular identification. The goal of this study was to test methods of rapidly killing fish embryos that maintain both morphological and molecular integrity. Rapid cooling with dry ice successfully maintained morphological and molecular integrity and may offer a simple and cost-effective approach for larval fish identification.
Topics: Animals; DNA Barcoding, Taxonomic; Dry Ice; Embryo, Nonmammalian; Fishes; Preservation, Biological
PubMed: 31893466
DOI: 10.1111/jfb.14248