-
Journal of Dentistry Jul 2022To evaluate the interactions of two phosphate ester monomers [10-methacryloyloxydecyl dihydrogen phosphate (10-MDP) and dipentaerythritol penta-acrylate phosphate...
OBJECTIVES
To evaluate the interactions of two phosphate ester monomers [10-methacryloyloxydecyl dihydrogen phosphate (10-MDP) and dipentaerythritol penta-acrylate phosphate (PENTA)] with hydroxyapatite and collagen and understand their influence on dentine bonding.
METHODS
Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, X-ray diffraction, nuclear magnetic resonance, ultraviolet-visible, and molecular docking were applied for separately evaluating the interactions of two monomers with hydroxyapatite and collagen. Hydrophilicity tests and morphological observation were employed to characterize pretreated dentine. Microtensile bond strength (μTBS) and nanoleakage were investigated to evaluate the bonding performance. Hydroxyproline assay, in situ zymography, and matrix metalloproteinase-9 (MMP-9) activity assay were used to confirm the MMP inhibition.
RESULTS
Chemoanalytic characterization confirmed the interactions of 10-MDP and PENTA with hydroxyapatite and collagen. The interactions of PENTA were weaker than 10-MDP. PENTA possessed better dentine tubule sealing after etching than 10-MDP. Dentine treated with PENTA was more hydrophilic than 10-MDP. 10-MDP and PENTA treating significantly increased the initial μTBS than the control group without primer conditioning. μTBS decreased significantly during aging, and the decrease was more severe in the PENTA group than 10-MDP. The 10-MDP and PENTA groups exhibited relatively less fluorescence than the control. The relative inhibition percentages of MMP-9 decreased in the order of 10-MDP-Ca salt, 10-MDP and PENTA. The 10-MDP, PENTA, and 10-MDP-Ca salt groups showed significantly lower hydroxyproline contents than the control.
CONCLUSIONS
Although PENTA adsorbed on hydroxyapatite, it did not form a stable calcium salt. The interactions of 10-MDP with hydroxyapatite and collagen are different than those of PENTA.
CLINICAL SIGNIFICANCE
The sealing of dentinal tubules by PENTA and the inhibition of MMP by 10-MDP and its calcium salts contribute to improving the dentine bonding durability.
Topics: Calcium; Dental Bonding; Dentin; Durapatite; Esters; Hydroxyproline; Materials Testing; Matrix Metalloproteinase 9; Methacrylates; Molecular Docking Simulation; Organophosphates; Resin Cements
PubMed: 35550398
DOI: 10.1016/j.jdent.2022.104159 -
Advanced Healthcare Materials Jan 2024Periodontitis, a chronic infection causing periodontal tissue loss, may be effectively addressed with in situ tissue engineering. Small intestinal submucosa (SIS) offers...
Hydroxyapatite-Coated Small Intestinal Submucosa Membranes Enhanced Periodontal Tissue Regeneration through Immunomodulation and Osteogenesis via BMP-2/Smad Signaling Pathway.
Periodontitis, a chronic infection causing periodontal tissue loss, may be effectively addressed with in situ tissue engineering. Small intestinal submucosa (SIS) offers exceptional biocompatibility and biodegradability but lacks sufficient osteoconductive and osteoinductive properties. This study develops and characterizes SIS coated with hydroxyapatite (SIS-HA) and gelatin methacrylate hydroxyapatite (SIS-Gel-HA) using biomineralization and chemical crosslinking. The impact on periodontal tissue regeneration is assessed by evaluating macrophage immune response and osteogenic differentiation potential of periodontal ligament stem cells (PDLSCs) in vitro and rat periodontal defects in vivo. The jejunum segment, with the highest collagen type I content, is optimal for SIS preparation. SIS retains collagen fiber structure and bioactive factors. Calcium content is 2.21% in SIS-HA and 2.45% in SIS-Gel-HA, with no significant differences in hydrophilicity, physicochemical properties, protein composition, or biocompatibility among SIS, SIS-HA, SIS-Gel, and SIS-Gel-HA. SIS is found to upregulate M2 marker expression, both SIS-HA and SIS-Gel-HA enhance the osteogenic differentiation of PDLSCs through the BMP-2/Smad signaling pathway, and SIS-HA demonstrates superior in vitro osteogenic activity. In vivo, SIS-HA and SIS-Gel-HA yield denser, more mature bones with the highest BMP-2 and Smad expression. SIS-HA and SIS-Gel-HA demonstrate enhanced immunity-osteogenesis coupling, representing a promising periodontal tissue regeneration approach.
Topics: Rats; Animals; Osteogenesis; Durapatite; Cell Differentiation; Periodontal Ligament; Signal Transduction; Immunity; Immunomodulation
PubMed: 37739439
DOI: 10.1002/adhm.202301479 -
Journal of Biomedical Materials... May 2022Hydroxyapatite (HAp)/collagen-based composite materials have been a constant in the development of bioinspired materials for bone tissue engineering. The most... (Review)
Review
Hydroxyapatite (HAp)/collagen-based composite materials have been a constant in the development of bioinspired materials for bone tissue engineering. The most fundamental research works focus on combining HAp, due to its chemical similarity with the mineral component of bones, and collagen, which is the most abundant protein in the body. Modern studies have explored different two-dimensional (2D) and 3D structures, in order to obtain biomaterials with specific physicochemical, mechanical, and biological characteristics that can be applied in distinct biomedical applications. However, as there is already so much work developed with these materials, it is crucial to question: what can still be done? What is the importance of current know-how for the future of bioinspired materials? In this paper we intend to review and update the available methodologies to synthesize HAp/collagen composites, along with their characteristics. In addition, the future of these materials in terms of applications and their potential as a cutting-edge technology is discussed.
Topics: Biocompatible Materials; Biomimetic Materials; Collagen; Durapatite; Tissue Engineering
PubMed: 34860461
DOI: 10.1002/jbm.b.34976 -
Journal of the Mechanical Behavior of... Apr 2023Hydroxyapatite (HAP), a natural constituent of bone tissue is commonly used for the clinical treatment of bone defects due to its similar structure with bone and...
Hydroxyapatite (HAP), a natural constituent of bone tissue is commonly used for the clinical treatment of bone defects due to its similar structure with bone and excellent biocompatibility. However, the processing exertion, poor osteoinductive capability and poor mechanical strength of HAP needs further addressing for its immense implementation in tissue engineering. Different approaches have been reported to escalate the mechanical hardness and osteogenic potential of HAP. In the present work, halloysite nanoclay (HNC) and sericin protein were used for better mechanical and osteogenic properties, respectively. Halloysite nanoclay (HNC, 1.0-4.0%) was used to reinforce hydroxyapatite (HAP) and the mechanical strength of nanocomposite scaffolds were evaluated. After surface modification of nanocomposite scaffolds with 1.0% silk sericin protein; physical properties like microstructure, porosity, swelling ratio and degradation rate were evaluated. Cell morphology, cytocompatibility and alkaline phosphatase (ALP) activity were assessed using MG 63 osteoblast cell lines. HAP reinforced with 4% HNC (HAP@4) showed a significant increase (199 MPa) in young modulus as compared to pure HAP. HAP reinforced with 2% HNC (HAP@2) and 4% HNC (HAP@4) showed a significant decrease in porosity as well as degradation rate than pure HAP but no significant difference was observed in swelling ratio. The scanning electron microscope (SEM) images of the scaffolds showed porous architecture. Remarkably, the incorporation of HNC in HAP enhanced the cytocompatibility as well as ALP activity in comparison to pure HAP. Overall, these results suggested that halloysite nanoclay reinforced HAP scaffold could be an auspicious alternative for bone tissue regeneration.
Topics: Durapatite; Tissue Scaffolds; Porosity; Clay; Sericins; Tissue Engineering; Bone Regeneration
PubMed: 36739825
DOI: 10.1016/j.jmbbm.2022.105626 -
Dental Materials : Official Publication... Jun 2022Targeting a tissue engineering-based vital pulp therapy (VPT), this study investigated the incorporation of nano-hydroxyapatite (nHA) into polycaprolactone (PCL)...
OBJECTIVES
Targeting a tissue engineering-based vital pulp therapy (VPT), this study investigated the incorporation of nano-hydroxyapatite (nHA) into polycaprolactone (PCL) nanofibers, and the metabolism of human dental pulp cells (HDPCs) seeded on the scaffolds.
METHODS
PCL-based solutions (10% w/v) containing nHA (0 - control; 0.5; 1.0; or 2.0% w/v) were electrospun into nanofibrous scaffolds. The scaffolds were characterized for morphology and composition (MEV/EDS), solubility, the release of calcium/phosphate (C/P), and modulation of medium pH. Then, HDPCs were seeded on the scaffolds and evaluated for cell viability (alamarBlue and live/dead), adhesion and spreading (F-actin), total protein (TP; Lowry), alkaline phosphatase activity (ALP; thymolphthalein assay), expression of odontogenic genes (RT-qPCR), and formation of a mineralized matrix (Alizarin Red). Data were analyzed with ANOVA and post-hocs (α = 5%).
RESULTS
Higher nHA concentrations roughened fiber surfaces, whereas PCL+ 2%nHA increased the interfibrillar spaces. PCL+ 1%nHA or PCL+ 2%nHA significantly released more C/P but the medium pH was maintained below 8.0. HDPCs viability was not affected by nHA, while cell adhesion/spreading was favored, especially for PCL+ 2%nHA. Higher protein content and ALP activity were seen for scaffolds incorporated with nHA, after 21 days. PCL+ 1%nHA and PCL+ 2%nHA upregulated the expression of DSPP and DMP1 in 14 days, and COL1A1, ALPL, and DMP1 in 21 days. The formation of a mineralized matrix was nHA concentration-dependent, and it was about 9 × higher for PCL+ 2%nHA.
SIGNIFICANCE
nHA-incorporated PCL nanofibrous scaffolds are cytocompatible and can stimulate the adhesion and odontogenic potential of HDPCs. PCL+ 2%nHA formulation is a bioactive tissue engineering-based cell-homing strategy for VPT.
Topics: Cell Differentiation; Cell Proliferation; Dental Pulp; Dentin; Durapatite; Humans; Nanofibers; Polyesters; Tissue Engineering; Tissue Scaffolds
PubMed: 35331551
DOI: 10.1016/j.dental.2022.03.006 -
Journal of Biomedical Materials... Feb 2021Bone tissue engineering is a promising approach for tackling clinical challenges. Osteoprogenitor cells, osteogenic factors, and osteoinductive/osteoconductive scaffolds...
Bone tissue engineering is a promising approach for tackling clinical challenges. Osteoprogenitor cells, osteogenic factors, and osteoinductive/osteoconductive scaffolds are employed in bone tissue engineering. However, scaffold materials remain limited due to their source, low biocompatibility, and so on. In this study, a composite hydrogel scaffold composed of hydroxyapatite (HA) and sodium alginate (SA) was manufactured using three-dimensional printing. Naringin (NG) and calcitonin-gene-related peptide (CGRP) were used as osteogenic factors in the fabrication of drug-loaded scaffolds. Investigation using animal experiments, as well as scanning electron microscopy, cell counting kit-8 testing, alkaline phosphatase staining, and alizarin red-D staining of bone marrow mesenchymal stem cell culture showed that the three scaffolds displayed similar physicochemical properties and that the HA/SA/NG and HA/SA/CGRP scaffolds displayed better osteogenesis than that of the HA/SA scaffold. Thus, the HA/SA scaffold could be a biocompatible material with potential applications in bone regeneration. Meanwhile, NG and CGRP doping could result in better and more positive proliferation and differentiation.
Topics: Alginates; Animals; Biocompatible Materials; Bone Marrow Cells; Bone Regeneration; Cell Adhesion; Cell Differentiation; Cell Proliferation; Dogs; Durapatite; Flavanones; Humans; Osteocytes; Osteogenesis; Printing, Three-Dimensional; Stem Cells; Tissue Scaffolds
PubMed: 32490561
DOI: 10.1002/jbm.a.37018 -
Colloids and Surfaces. B, Biointerfaces Nov 2022The bonding strength of the bone-titanium (Ti) implant interface is critical for patients undergoing joint replacement. However, current bone adhesives used in clinic...
The bonding strength of the bone-titanium (Ti) implant interface is critical for patients undergoing joint replacement. However, current bone adhesives used in clinic have shortcomings, such as biological inertness, cytotoxicity, and lack of osteogenic ability. In this study, a simple and low-cost hydrogel-based bone adhesive was prepared to improve the osseointegration ability and anti-infection ability of the bone-implant interface. A multifunctional hydrogel was prepared by incorporating nano-hydroxyapatite (HA) on polyethyleneimine (PEI) and polyacrylic acid (PAA) (PEI/PAA-HA). It was shown that PEI/PAA-HA hydrogel exhibited good self-healing and strong adhesive ability. The adhesive strengths of bone-Ti and Ti-Ti were measured as 2.30 ± 0.15 MPa and 1.07 ± 0.07 MPa, respectively. Vancomycin (VAN) was loaded into the PEI/PAA-HA hydrogel (PEI/PAA-HA-VAN) via a simple immersion method. The PEI/PAA-HA-VAN showed excellent antibacterial effect by sustained release of VAN. In addition, the PEI/PAA-HA-VAN hydrogel exhibited excellent cytocompatibility promoting the expression of osteogenic genes and the deposition of mineralized matrix. Collectively, this strong adhesive hydrogel showed great potential in enhancing bone-implant interface fixation.
Topics: Humans; Bone-Implant Interface; Hydrogels; Durapatite; Osteogenesis; Osseointegration
PubMed: 36084513
DOI: 10.1016/j.colsurfb.2022.112817 -
Sensors (Basel, Switzerland) Dec 2022In this paper, a novel nanocrystalline composite material of hydroxyapatite (HA)/polyvinyltrimethoxysilane (PVTMS)/iron(II)chloride tetrahydrate (ClFeH-O) with hexagonal...
In this paper, a novel nanocrystalline composite material of hydroxyapatite (HA)/polyvinyltrimethoxysilane (PVTMS)/iron(II)chloride tetrahydrate (ClFeH-O) with hexagonal structure is proposed for the fabrication of a gas/temperature sensor. Taking into account the sensitivity of HA to high temperatures, to prevent the collapse and breakdown of bonds and the leakage of volatiles without damaging the composite structure, a freeze-drying machine is designed and fabricated. X-ray diffraction, FTIR, SEM, EDAX, TEM, absorption and photoluminescence analyses of composite are studied. XRD is used to confirm the material structure and the crystallite size of the composite is calculated by the Monshi-Scherrer method, and a value of 81.60 ± 0.06 nm is obtained. The influence of the oxygen environment on the absorption and photoluminescence measurements of the composite and the influence of vaporized ethanol, N and CO on the SiO/composite/Ag sensor device are investigated. The sensor with a 30 nm-thick layer of composite shows the highest response to vaporized ethanol, N and ambient CO. Overall, the composite and sensor exhibit a good selectivity to oxygen, vaporized ethanol, N and CO environments.
Topics: Durapatite; Temperature; Silicon Dioxide; Nanocomposites; Oxygen; Ethanol
PubMed: 36560381
DOI: 10.3390/s222410012 -
Journal of Materials Science. Materials... Jul 2023The present work studies the effect of Mn doping on the crystalline structure of the Hap synthesized by the hydrothermal method at 200 °C for 24 h, from Ca(OH) and...
The present work studies the effect of Mn doping on the crystalline structure of the Hap synthesized by the hydrothermal method at 200 °C for 24 h, from Ca(OH) and (NH)HPO, incorporating MnCl to 0.1, 0.5, 1.0, 1.5 and 2.0 %wt of Mn concentrations. Samples were characterized by the X-Ray Diffraction technique, which revealed the diffraction peaks that corresponded to the hexagonal and monoclinic phase of the Hap; it was observed that the average size of crystallite decreased from 23.67 to 22.69 nm as the concentration of Mn increased. TEM shows that in all samples, there are two distributions of particle sizes; one corresponds to nanorods with several tens of nanometers in length, and the other in which the diameter and length are very close. FTIR analysis revealed absorption bands corresponding to the PO and OH groups characteristic of the Hap. It was possible to establish a substitution mechanism between the Mn and the ions of Ca of the Hap. From the Alamar blue test, a cell viability of 86.88% ± 5 corresponding to the sample of Hap at 1.5 %wt Mn was obtained, considered non-cytotoxic according to ISO 10993-5. It also evaluated and demonstrated the good osteoinductive properties of the materials, which were verified by histology and immunofluorescence expression of osteogenic markers. Adhesion, viability, biocompatibility and osteoinductive properties, make these materials candidates for future applications in bone tissue engineering with likely uses in regenerative medicine.
Topics: Durapatite; Nanotubes; Osteogenesis
PubMed: 37515640
DOI: 10.1007/s10856-023-06744-0 -
Nanoscale Aug 2022Biomineralization is a common strategy used in Nature to improve the mechanical strength and toughness of biological materials. This strategy, applied in materials like...
Biomineralization is a common strategy used in Nature to improve the mechanical strength and toughness of biological materials. This strategy, applied in materials like bone or nacre, serves as inspiration for materials scientists and engineers to design new materials for applications in healthcare, soft robotics or the environment. In this regard, composites consisting of silk and hydroxyapatite have been extensively researched for bone regeneration applications, due to their reported cytocompatibility and osteoinduction capacity that supports bone formation . Thus, it becomes relevant to understand how silk and hydroxyapatite interact at their interface, and how this affects the overall mechanical properties of these composites. This theoretical-experimental work investigates the interfacial dynamic and structural properties of silk in contact with hydroxyapatite, combining molecular dynamics simulations with analytical characterization. Our data indicate that hydroxyapatite decreases the β-sheets in silk, which are a key load-bearing element of silk. The β-sheets content can usually be increased in silk biomaterials post-processing methods, such as water vapor annealing. However, the presence of hydroxyapatite appears to reduce also for the formation of β-sheets water vapor annealing. This work sheds light into the interfacial properties of silk-hydroxyapatite composite and their relevance for the design of composite biomaterials for bone regeneration.
Topics: Biocompatible Materials; Bone Regeneration; Durapatite; Silk; Steam
PubMed: 35852800
DOI: 10.1039/d2nr01989b