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ACS Applied Materials & Interfaces Oct 2023One of the most critical issues concerning orthopedic implants is the risk of chronic inflammation, which poses a threat to the bone healing process....
One of the most critical issues concerning orthopedic implants is the risk of chronic inflammation, which poses a threat to the bone healing process. Osteo-immunomodulation plays a pivotal role in implant technology by influencing proinflammatory and anti-inflammatory responses, ultimately promoting bone healing. This study aims to investigate the morphology-dependent osteo-immunomodulatory properties of a hydroxyapatite (HA)/plasma electrolytic oxidation (PEO)-coated WE43 alloy. In this context, following the PEO process with various operational parameters (duty cycles of 50-40, 50-20, 70-40%, and frequencies of 0.5, 0.8, and 1 kHz), a layer of HA was applied as the top coating using a straightforward hot-dip process. The results revealed the formation of the PEO layer with distinct morphologies and pore sizes, depending on the operational parameters. Specifically, a uniform PEO coating with small pore sizes (5.2-5.3 μm) led to the creation of plate-like HA particles, while a random-like HA structure formed on nonuniform surfaces with large pores (7.0-11.1 μm) of PEO. Moreover, it was observed that the plate-like HA coating exhibited higher adhesion strength than the random one (classified as class 2 vs class 3 based on cross-cut standards). Furthermore, electrochemical impedance spectroscopy (EIS) and polarization studies confirmed a substantial increase in the polarization resistance (680 kΩ) and total impedance (48 559.6 Ω) for the plate-like HA/PEO as compared to the substrate (an increase of 1511-fold and 311-fold, respectively) and the random HA/PEO samples (an increase of 85-fold and 18-fold, respectively). In addition, compared to random HA coatings, there was a significant enhancement in the viability (150% control vs 96% control), proliferation, and differentiation of MG63 cells when exposed to plate-like HA coatings. Moreover, surface morphology and chemistry pronouncedly impacted macrophages' viability, morphology, and phenotype. Notably, plate-like HA coatings resulted in a higher upregulation of BMP-2 and TGF-β than proinflammatory cytokines (IL-6 and M-CSF), indicating a polarization of macrophage type 1 (M1) toward type 2 (M2). In summary, the bilayer HA/PEO coating exhibited remarkable osteo-immunomodulatory activity, making it highly appealing for use in bone implant applications.
Topics: Magnesium; Durapatite; Surface Properties; Prostheses and Implants; Bone and Bones; Coated Materials, Biocompatible; Titanium
PubMed: 37831072
DOI: 10.1021/acsami.3c11184 -
Macromolecular Bioscience Nov 2022Bone tissue is usually damaged after big traumas, tumors, and increasing aging-related diseases such as osteoporosis and osteoarthritis. Current treatments are based on...
Bone tissue is usually damaged after big traumas, tumors, and increasing aging-related diseases such as osteoporosis and osteoarthritis. Current treatments are based on implanting grafts, which are shown to have several inconveniences. In this regard, tissue engineering through the 3D bioprinting technique has arisen to manufacture structures that would be a feasible therapeutic option for bone regenerative medicine. In this study, nanocellulose-alginate (NC-Alg)-based bioink is improved by adding two different inorganic components such as hydroxyapatite (HAP) and graphene oxide (GO). First, ink rheological properties and biocompatibility are evaluated as well as the influence of the sterilization process on them. Then, scaffolds are characterized. Finally, biological studies of embedded murine D1 mesenchymal stem cells engineered to secrete erythropoietin are performed. Results show that the addition of both HAP and GO prevents NC-Alg ink from viscosity lost in the sterilization process. However, GO is reduced due to short cycle autoclave sterilization, making it incompatible with this ink. In addition, HAP and GO have different influences on scaffold architecture and surface as well as in swelling capacity. Scaffolds mechanics, as well as cell viability and functionality, are promoted by both elements addition. Additionally, GO demonstrates an enhanced bone differentiation capacity.
Topics: Animals; Mice; Durapatite; Printing, Three-Dimensional; Bioprinting; Tissue Engineering; Bone Regeneration; Alginates; Tissue Scaffolds
PubMed: 35981208
DOI: 10.1002/mabi.202200236 -
European Spine Journal : Official... Jul 2023To develop a novel 3D printable polyether ether ketone (PEEK)-hydroxyapatite (HA)-magnesium orthosilicate (MgSiO) composite material with enhanced properties for...
PURPOSE
To develop a novel 3D printable polyether ether ketone (PEEK)-hydroxyapatite (HA)-magnesium orthosilicate (MgSiO) composite material with enhanced properties for potential use in tumour, osteoporosis and other spinal conditions. We aim to evaluate biocompatibility and imaging compatibility of the material.
METHODS
Materials were prepared in three different compositions, namely composite A: 75 weight % PEEK, 20 weight % HA, 5 weight % MgSiO; composite B: 70 weight% PEEK, 25 weight % HA, 5 weight % MgSiO; and composite C: 65 weight % PEEK, 30 weight % HA, 5 weight % MgSiO. The materials were processed to obtain 3D printable filament. Biomechanical properties were analysed as per ASTM standards and biocompatibility of the novel material was evaluated using indirect and direct cell cytotoxicity tests. Cell viability of the novel material was compared to PEEK and PEEK-HA materials. The novel material was used to 3D print a standard spine cage. Furthermore, the CT and MR imaging compatibility of the novel material cage vs PEEK and PEEK-HA cages were evaluated using a phantom setup.
RESULTS
Composite A resulted in optimal material processing to obtain a 3D printable filament, while composite B and C resulted in non-optimal processing. Composite A enhanced cell viability up to ~ 20% compared to PEEK and PEEK-HA materials. Composite A cage generated minimal/no artefacts on CT and MR imaging and the images were comparable to that of PEEK and PEEK-HA cages.
CONCLUSION
Composite A demonstrated superior bioactivity vs PEEK and PEEK-HA materials and comparable imaging compatibility vs PEEK and PEEK-HA. Therefore, our material displays an excellent potential to manufacture spine implants with enhanced mechanical and bioactive property.
Topics: Humans; Durapatite; Polyethylene Glycols; Polymers; Ketones
PubMed: 37179256
DOI: 10.1007/s00586-023-07734-0 -
Biomaterials Advances Feb 2022Customisation of bioactivity and degradability of porous bioceramic scaffolds is a formidable challenge in the field of regenerative medicine. In this study, we...
Customisation of bioactivity and degradability of porous bioceramic scaffolds is a formidable challenge in the field of regenerative medicine. In this study, we developed gyroid-structured ternary composite scaffolds (biphasic calcium phosphate (BCP) and 45S5 bioglass® (BG)) using digital light processing 3D printing technology based on material and structural design. Additionally, the mechanical strength, bioactivity, degradability, and biocompatibility of the composite ceramic scaffolds were evaluated. The results revealed that BG reacted with BCP to generate major active crystalline phases of CaSiO and NaCa(PO). These active crystalline phases accelerated the exchange rate of Si, Ca, and PO with HCO in simulated body fluids and resulted in the rapid formation of carbonated hydroxyapatite (CHA), analogous to the formation of natural bone tissue. Interestingly, the precipitated CHA showed petal- and needle-like morphologies, which provided a large surface area to promote cell adhesion and proliferation. Furthermore, an increase in the BG content improved the degradability of ternary composite scaffolds after soaking in Tris-HCl solution. The tuneable degradability increased by three times at 30 wt% BG and sharply increased by 6.8 times at 40 wt% BG. This study provides a promising strategy to design scaffolds with improved bioactivity and tuneable degradability to assist a diverse population suffering from orthopedic conditions.
Topics: Bone and Bones; Durapatite; Porosity; Printing, Three-Dimensional; Tissue Scaffolds
PubMed: 35527154
DOI: 10.1016/j.msec.2021.112595 -
Journal of the Mechanical Behavior of... Jun 2022Human tooth enamel is composed of enamel rods and surrounding inter-rod enamel. As the fundamental block of enamel, hydroxyapatite (HAP) nanofibers are mostly...
Human tooth enamel is composed of enamel rods and surrounding inter-rod enamel. As the fundamental block of enamel, hydroxyapatite (HAP) nanofibers are mostly longitudinally aligned in the rods but inclined in the inter-rod enamel. The surface hardening of enamel by occlusal loading is reportedly a result of hydroxyapatite nanofiber fragmentation and rearrangement and plays an important role in the anti-wear performance of enamel, but little is known about the effect of HAP nanofiber orientation on enamel surface hardening. In this study, the occlusal loading-induced surface hardening behaviors of enamel at different zones (rod and inter-rod) and different orientations (occlusal and axial) were investigated in vitro using impact treatment and a nanoindentation technique, aiming to reveal the effect of nanofiber orientation on enamel surface hardening. It was found that surface hardening by occlusal loading occurs in the rod and inter-rod areas, but the former shows a greater hardening degree than the latter, leading to an increase in the mechanical heterogeneity of enamel surface. Under the same loading condition, the HAP nanofibers in the inter-rod enamel are more likely to tilt into transverse nanofibers than those in the rods. Compared with longitudinal nanofibers, transverse nanofibers fragment into more transverse crystal particles, but the transverse particles impair the compactness of the hardening layer and decrease its hardening degree. In sum, inherent non-uniform nanofiber orientation endows the enamel with the ability to undergo heterogeneous surface hardening upon occlusal loading, which is critical for providing and maintaining its surface mechanical heterogeneity. These findings extend the understanding of the relationship between microstructure and mechanical properties of dental enamel and provide valuable insights into the bionic design of engineering materials.
Topics: Dental Enamel; Durapatite; Humans; Nanofibers; Tooth
PubMed: 35405521
DOI: 10.1016/j.jmbbm.2022.105221 -
Acta Biomaterialia Jun 2022Concerns about the potential systematic toxicity limit the extensive application of traditional therapeutic drugs for melanoma therapy, nano-hydroxyapatite (nHA) with...
Concerns about the potential systematic toxicity limit the extensive application of traditional therapeutic drugs for melanoma therapy, nano-hydroxyapatite (nHA) with good biocompatibility and anti-tumor ability could be an alternative choice. In this study, nHA was employed as an anti-tumor biomaterial due to its tumor-specific toxicity. Meanwhile, granulocyte-macrophage colony-stimulating factor (GM-CSF) served as the immune adjuvant to activate the immune response. The delivery platform was fabricated by co-encapsulation of both nHA and GM-CSF into a biocompatible thermosensitive PLGA-PEG-PLGA hydrogel. The results showed that the bio-activities of nHA and GM-CSF could be well-maintained within the hydrogel. Interestingly, the addition of nHA could attenuate the burst release of GM-CSF due to possible protein absorption capacity of nHA, which is beneficial for GM-CSF sustainable release at the tumor site, achieving boosted and prolonged anti-tumor immunity. The in vitro and in vivo data demonstrated that nHA/GM-CSF hydrogel exhibited greater potency to inhibit tumor growth via enhanced CD8 T-cell response compared with hydrogel and nHA hydrogel groups, contributed by the synergistic effects of nHA and GM-CSF. Overall, the strategy combining nHA and immune adjuvant shows great promise, which largely broadens the choice of combinational therapies for melanoma. STATEMENT OF SIGNIFICANCE: Nano-hydroxyapatite (nHA) has been confirmed to specifically inhibit melanoma tumor growth and induce immune response. However, its antitumor efficiency and immunity-evoking capacity are limited. In this study, granulocyte-macrophage colony-stimulating factor (GM-CSF) was introduced to serve as the immune adjuvant. Both of them were encapsulated into a biocompatible thermosensitive PLGA-PEG-PLGA hydrogel. The addition of nHA could attenuate the burst release of GM-CSF due to the interaction with nHA, which is beneficial for GM-CSF sustainable release at tumor site, achieving boosted and prolonged anti-tumor immunity. Anti-tumor immune response could be activated due to the release of tumor-associated antigen and tumor debris induced by the specifically tumor inhibition effect of nHA and GM-CSF. The combination of nHA and GM-CSF could play synergistic inhibiting effect on tumor growth via boosting and prolonging anti-tumor immunity.
Topics: Adjuvants, Immunologic; Durapatite; Granulocyte-Macrophage Colony-Stimulating Factor; Humans; Hydrogels; Immunity, Cellular; Melanoma
PubMed: 35398268
DOI: 10.1016/j.actbio.2022.04.002 -
International Journal of Molecular... Apr 2022Collagen I-based foams were modified with calcined or noncalcined hydroxyapatite or calcium phosphates with various particle sizes and pores to monitor their effect on...
Collagen I-based foams were modified with calcined or noncalcined hydroxyapatite or calcium phosphates with various particle sizes and pores to monitor their effect on cell interactions. The resulting scaffolds thus differed in grain size, changing from nanoscale to microscopic, and possessed diverse morphological characteristics and resorbability. The materials' biological action was shown on human bone marrow MSCs. Scaffold morphology was identified by SEM. Using viability test, qPCR, and immunohistochemical staining, we evaluated the biological activity of all of the materials. This study revealed that the most suitable scaffold composition for osteogenesis induction is collagen I foam with calcined hydroxyapatite with a pore size of 360 ± 130 µm and mean particle size of 0.130 µm. The expression of osteogenic markers RunX2 and ColI mRNA was promoted, and a strong synthesis of extracellular protein osteocalcin was observed. ColI/calcined HAP scaffold showed significant osteogenic potential, and can be easily manipulated and tailored to the defect size, which gives it great potential for bone tissue engineering applications.
Topics: Cell Differentiation; Cells, Cultured; Collagen Type I; Durapatite; Humans; Osteogenesis; Tissue Engineering; Tissue Scaffolds
PubMed: 35457055
DOI: 10.3390/ijms23084236 -
Journal of the Mechanical Behavior of... Feb 2024Hydroxyapatite (HAp) is a ceramic composed of calcium phosphate, frequently employed as a bone substitute material due to its biocompatibility and bioactivity. Over the...
Hydroxyapatite (HAp) is a ceramic composed of calcium phosphate, frequently employed as a bone substitute material due to its biocompatibility and bioactivity. Over the past century, there has been substantial attention in fields such as orthopedics and plastic surgery. Remarkably, synthetic HAp exhibits properties akin to those found in natural bone and teeth. Computational theoretical chemistry focuses on numerically computing molecular electronic structures and interactions. As chemistry education evolves, it's imperative to acknowledge the increasing significance of computational tools in research. Density Functional Theory (DFT) stands out as the most widely adopted method in contemporary computational chemistry. In this study, we synthesized Hydroxyapatite (HAp) via the double decomposition method using synthetic sources. The synthesized materials underwent thorough characterization, including X-ray Diffraction (XRD), UV-visible spectroscopy, and Fourier Transform Infrared (FTIR) spectroscopy under various conditions. Additionally, we performed quantum mechanical computations on the HAp molecule using density functional theory. Our results were then compared with experimental data. Our experimental findings highlight the successful synthesis of HAp, particularly under specific temperature conditions. Moreover, the quantum chemistry calculations exhibited excellent agreement with the experimental results, especially in terms of spectroscopic characterizations.
Topics: Durapatite; Bone and Bones; Spectroscopy, Fourier Transform Infrared
PubMed: 38000164
DOI: 10.1016/j.jmbbm.2023.106229 -
European Journal of Pharmaceutical... Jan 2024As an attractive biomaterial for bone reconstruction, injectable biomaterials have many prominent characteristics such as good biocompatibility and bone-filling ability....
As an attractive biomaterial for bone reconstruction, injectable biomaterials have many prominent characteristics such as good biocompatibility and bone-filling ability. However, there are weak as load-bearing scaffolds. In this study, polyvinyl alcohol (PVA) and bioactive glass (BAG) were interpenetrated into sodium alginate (SA) network to obtain self-enhanced injectable hydrogel. The optimum ratio of PVA/SA/BAG hydrogel was determined based on injectability, gelation time and chemical characterization. Results showed that the selected ratio had the shortest gelation time of 3.5min, and the hydrogel had a rough surface and good coagulation property. The hydrogel was capable of carrying 1kg of weight by mineralization for 14 d The compressive strength, compressive modulus, and fracture energy of the hydrogel reached 0.12MPa, 0.376MPa and 17.750kJ m, respectively. Meanwhile, the hydrogel had high moisture content and dissolution rate, and it was sensitive to temperature and ionic strength. Hydroxyapatite was generated on the hydrogel surface, and the hydrogel pores increased, and the pore size enlarged. The biocompatibility of PVA/SA/BAG hydrogel was analyzed using hemolysis and cytotoxicity assays. Results revealed its good biocompatibility with low hemolysis rate and no cytotoxicity to MC3T3-E1 cells. The hydrogel was also found to promote the differentiation of MC3T3-E1 cells with significantly increased in ALP activity and expression of relevant differentiation factors. In vitro mineralization assay showed an increase in calcium nodules and calcification area, indicating the ability of hydrogel to promote mineralization MC3T3-E1 cells. These findings indicated that PVA/SA/BAG hydrogel had potential uses in the field of irregular bone-defect repair due to its injectability, cytocompatibility, and tailorable functionality.
Topics: Humans; Hydrogels; Hemolysis; Biocompatible Materials; Durapatite; Cell Differentiation
PubMed: 37865283
DOI: 10.1016/j.ejps.2023.106617 -
International Journal of Biological... May 2023Biopolymer/hydroxyapatite (HAp) composites are one type of the most promising materials for a variety of biomedical applications. In this study, hierarchical and...
Biopolymer/hydroxyapatite (HAp) composites are one type of the most promising materials for a variety of biomedical applications. In this study, hierarchical and urchin-like chitosan/HAp nanowire (HU-CS/HAp NW) microspheres were for the first time synthesized by in situ hydrothermal treatment of chitosan/HAp (CS/HAp) microspheres in the acetic acid solution. The results indicate that HU-CS/HAp NW microspheres were spherical in morphology with a diameter of 100-300 μm. Their surface was mainly constructed by numerous HAp NWs with the diameter of 80-120 nm and showed a hierarchical and urchin-like nanofibrous architecture. It was found that the acidic hydrothermal treatment caused an in situ conversion of HAp NPs to HAp NWs. In vitro biocompatible evaluation indicates that HU-CS/HAp NW microspheres showed an enhanced cell attachment and proliferation due to the presence of hierarchical and urchin-like architecture. Furthermore, HU-CS/HAp NW microspheres showed a good adsorption capacity for tetracycline hydrochloride (model drug, one of the most representative antibiotics) with a higher adsorption capacity than CS/HAp microspheres and well maintained their antibacterial efficacy to inhibit the growth of bacteria: Escherichia coli and Staphylococcus aureus. Thus, the present HU-CS/HAp NW microspheres would be applicable as novel drug-laden cell carriers.
Topics: Durapatite; Chitosan; Drug Carriers; Microspheres; Anti-Bacterial Agents
PubMed: 36921830
DOI: 10.1016/j.ijbiomac.2023.124039