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Physical Chemistry Chemical Physics :... Dec 2023Liquid water is essential for life as we know it and the coupling between water and biomolecular dynamics is crucial for life processes. Jupiter's moon Europa is a good...
Liquid water is essential for life as we know it and the coupling between water and biomolecular dynamics is crucial for life processes. Jupiter's moon Europa is a good candidate for searching for extraterrestrial life in our outer solar system, mainly because a liquid water salty ocean in contact with a rocky seafloor underlies its ice shell. Little, however, is known about the chemical composition of the subglacial ocean of Europa or the brine pockets within its ice shell and their impacts on water dynamics. Here, we employ H, O, Na and Cl NMR spectroscopy, especially NMR spin relaxation and diffusion methods, and investigate the mobility of water molecules and ions in eutectic solutions of magnesium sulfate and sodium chloride, two salts ubiquitously present on the surface of Europa, over a range of temperatures and pressures pertinent to Europa's subglacial ocean. The NMR data demonstrate the more pronounced effect of magnesium sulfate compared with sodium chloride on the mobility of water molecules. Even at its much lower eutectic temperature, the sodium chloride solution retains a relatively large level of water mobility. Our results highlight the higher potential of a sodium chloride-rich than magnesium sulfate-rich Europa's ocean to accommodate life and support life origination within the eutectic melts of Europa's ice shell.
PubMed: 38054803
DOI: 10.1039/d3cp03455k -
Behavioural Brain Research Sep 2021Evidence suggest that magnesium dietary supplementation has several health benefits including lowering blood pressure, reducing insulin resistance, and improving...
Evidence suggest that magnesium dietary supplementation has several health benefits including lowering blood pressure, reducing insulin resistance, and improving symptoms of depression, anxiety, and migraine. Here, we aimed to study the effect of chronic magnesium supplementation on anxiety-like behavior in rats by supplementing with magnesium their drinking water for 30 days. Anxiety-like behavior was induced by subcutaneous injection of veratrin 30 min before performing elevated plus maze and open field tests to measure anxiety levels and locomotion, respectively. We quantify the concentration of magnesium in plasma and cerebrospinal fluid. We used diazepam to compare the efficacy of magnesium supplementation as an anxiolytic agent. Our results show that rats supplemented with magnesium had a statistically significant decrease in anxiety levels with not effects on locomotion and a statistically significant increase in concentration of magnesium in plasma and cerebrospinal fluid. However, the anxiolytic effect of magnesium supplementation washes-out in 12 days. We discuss the advantages of using supplemental magnesium as anxiolytic.
Topics: Animals; Anti-Anxiety Agents; Anxiety; Behavior, Animal; Diazepam; Disease Models, Animal; Magnesium; Magnesium Chloride; Rats; Rats, Wistar
PubMed: 34252502
DOI: 10.1016/j.bbr.2021.113460 -
Antioxidants (Basel, Switzerland) Dec 2023Anesthesia serves as an effective method to mitigate the stress response in aquatic animals during aquaculture and product transportation. In this study, we assessed the...
Anesthesia serves as an effective method to mitigate the stress response in aquatic animals during aquaculture and product transportation. In this study, we assessed the anesthetic efficacy of clove oil, tricaine methane-sulfonate (MS-222), ethanol, and magnesium chloride by anesthesia duration, recovery time, 24-hour survival rate, and the behavior of mud crabs (). Additionally, the optimal anesthetic concentration for varying body weights of mud crabs was also investigated. The results revealed that clove oil emerged as the optimal anesthetic for mud crabs, with a 24-hour survival rate surpassing those observed in MS-222 and magnesium chloride treatments. Ethanol caused amputation and hyperactivity in mud crabs. Regression analyses between the optimal anesthetic concentration of clove oil and the weight categories of 0.03-27.50 g and 27.50-399.73 g for mud crabs yielded the following equations: y = 0.0036 x - 0.1629 x + 1.7314 x + 4.085 (R = 0.7115) and y = 0.0437 x + 2.9461 (R = 0.9549). Clove oil exhibited no significant impact on serum cortisol, glucose, lactate content, aspartate aminotransferase (AST), alanine aminotransferase (ALT) activities, or superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) levels in mud crabs across different treatment groups. Anesthesia induced by clove oil in mud crabs resulted in an increase in inhibitory neurotransmitters such as glycine. However, the recovery from anesthesia was associated with elevated levels of the excitatory neurotransmitters L-aspartic acid and glutamate. In conclusion, clove oil proves to be a safe and optimal anesthetic agent for mud crabs, exerting no physiological stress on the species.
PubMed: 38136243
DOI: 10.3390/antiox12122124 -
Nano Letters Oct 2021Magnesium bis(hexamethyldisilazide) (Mg(HMDS))-based electrolytes are compelling candidates for rechargeable magnesium batteries due to their high compatibility with...
Magnesium bis(hexamethyldisilazide) (Mg(HMDS))-based electrolytes are compelling candidates for rechargeable magnesium batteries due to their high compatibility with magnesium metal anode. However, the usual combination of Mg(HMDS) with chloride salts limits their practical application due to severe corrosion of cell components and low anodic stability. Herein, we report for the first time, a chloride-free Mg(HMDS)-based electrolyte in 1,2-dimethoxyethane. By chemically controlling the moisture content using tetrabutylammonium borohydride as a moisture scavenger, the electrolyte demonstrates outstanding electrochemical performance in magnesium plating/stripping, with an average Coulombic efficiency of 98.3% over 150 cycles, and is noncorrosive to cell components. Surface analysis and depth profiling of the magnesium metal anode reveals the formation of a robust solid electrolyte interphase at the anode-electrolyte nanointerface, which allows magnesium plating/stripping to occur reversibly. The electrolyte also demonstrates good compatibility with a copper sulfide nanomaterial cathode, which exhibits a high initial discharge capacity of 261.5 mAh g.
PubMed: 34519512
DOI: 10.1021/acs.nanolett.1c02655 -
Journal of Feline Medicine and Surgery Dec 2022The aim of this study was to evaluate the magnitude, duration and significance of postprandial changes to select serum biochemistry analytes in healthy adult cats in the...
OBJECTIVES
The aim of this study was to evaluate the magnitude, duration and significance of postprandial changes to select serum biochemistry analytes in healthy adult cats in the 12 h period after a meal.
METHODS
Nine adult research cats fed commercial food were included. Blood samples were taken after a 12 h fast (hour 0), cats were offered and consumed a meal, and postprandial samples were obtained over a 12 h period starting 2 h after the baseline blood draw (hours 2, 4, 6, 8, 10 and 12). Serum samples were run on a Roche Cobas C501 chemistry analyzer to obtain concentrations of blood urea nitrogen (BUN), creatinine, phosphorus, total calcium, bicarbonate, cholesterol, magnesium, sodium, potassium and chloride. Serum concentrations of each analyte at hours 2, 4, 6, 8, 10 and 12 were compared with concentrations prior to feeding.
RESULTS
Serum concentration for at least one postprandial time point was different from baseline fasted concentration for BUN (hour 2, = 0.006; hour 4, <0.0001; hour 6, = 0.002; hour 8, = 0.026), phosphorus (hour 2, = 0.019), bicarbonate (hours 2, 4, 6, 8 and 10; all <0.01), glucose (hour 12, = 0.014), magnesium (hour 10, = 0.029) and chloride (hour 2, = 0.026; hour 4, = 0.044; hour 12, = 0.019). No significant difference was seen at any postprandial sampling point compared with baseline for serum creatinine, total calcium, cholesterol, sodium or potassium concentrations.
CONCLUSIONS AND RELEVANCE
Short-term postprandial serum concentrations of BUN, phosphorus, bicarbonate and chloride differed at multiple time points within a 12 h period compared with the fasted state at baseline, with most values remaining within the reference intervals. Veterinarians should be aware of these alterations, though they are unlikely to be mistaken for pathological disease states in healthy adult cats.
Topics: Cats; Animals; Bicarbonates; Chlorides; Magnesium; Calcium; Sodium; Potassium
PubMed: 36219454
DOI: 10.1177/1098612X221121929 -
Medicina (Kaunas, Lithuania) Sep 2022Vortioxetine (VRT) is a relatively new selective serotonin reuptake inhibitor (SSRI) antidepressant and serotonin receptor modulator, approved for the treatment of...
Vortioxetine (VRT) is a relatively new selective serotonin reuptake inhibitor (SSRI) antidepressant and serotonin receptor modulator, approved for the treatment of major depression and generalized anxiety disorder. Depression has been linked with psychomotor disengagement, oxidative stress burden and decreased blood levels of brain-derived neurotrophic factor (BDNF). In our study we performed the experimental investigation of VRT, magnesium and of their association on the rats' endurance capacity, motor behavior and blood biological disturbances in rats subjected to forced exercise in treadmill test. The substances were administered orally for 14 consecutive days, as follows: group 1 (control): distilled water 0.3 mL/100 g body; group 2 (Mg): magnesium chloride 200 mg/kg body; group 3 (VRT): VRT 20 mg/kg body; group 4 (VRT+Mg): VRT 20 mg/kg body + magnesium chloride 200 mg/kg body. Magnesium was used as positive control substance with known effects in treadmill test. The consequences of VRT treatment on glucose, cortisol, BDNF and oxidative stress biomarkers (superoxide-dismutase, malondialdehyde, glutathione-peroxidase, lactate dehydrogenase) were also assessed. The use of VRT resulted in an improvement in motor capacity and an increase of the rats' endurance to physical effort. The administration of VRT increased the serum BDNF levels and reduced the oxidative stress in rats subjected to physical effort. The association of magnesium potentiated the effects of VRT on physical performances, the antioxidant activity and the decreasing in serum stress markers in treadmill test in rats.
Topics: Rats; Animals; Brain-Derived Neurotrophic Factor; Vortioxetine; Magnesium; Selective Serotonin Reuptake Inhibitors; Antioxidants; Magnesium Chloride; Hydrocortisone; Superoxides; Glutathione Peroxidase; Malondialdehyde; Oxidative Stress; Antidepressive Agents; Biomarkers; Glutathione; Physical Functional Performance; Glucose; Lactate Dehydrogenases; Water
PubMed: 36295524
DOI: 10.3390/medicina58101363 -
Cureus Nov 2023Alzheimer's disease (AD) is a widespread neurodegenerative condition with complex causes and a significant global impact, particularly among the elderly. This brief...
INTRODUCTION
Alzheimer's disease (AD) is a widespread neurodegenerative condition with complex causes and a significant global impact, particularly among the elderly. This brief introduction emphasizes AD's hallmark features and the urgent public health concern it poses, with numbers on the rise. It also highlights the potential of statins and magnesium L-threonate as a combined therapeutic approach to prevent AD and mitigate its underlying pathological features. The study's goal is to shed light on these promising interventions in a rat model induced by aluminum chloride (AlCl3).
MATERIALS AND METHODS
A total of 30 aged female Wistar rats were divided into five groups (n=6/group). The vehicle control group received normal saline orally (p.o.).The model control group received AlCl3(4.2 mg/kg/day intraperitoneal (i.p.)). The standard-treated group received rivastigmine (1 mg/kg/day p.o.), and the atorvastatin-treated and atorvastatin with magnesium L-threonate-treated groups received atorvastatin (20 mg/kg/day p.o.) and atorvastatin (20 mg/kg/day) with magnesium L-threonate (604 mg/kg/day ), respectively. Cognitive functions such as radial arm maze, elevated plus maze (EPM), passive shock avoidance test, and open-field test (OFT) were performed at weekly intervals up to 28 days. After completion of the study on the 29 day, all animals were sacrificed, and the brain was used for estimation of AchE enzyme activity, oxidative stress parameters, and histopathological analysis.
RESULT
At the end of the fourth week, administration of atorvastatin and atorvastatin with magnesium L-threonate resulted in a decreased average time taken to reach the correct arm, reduced transfer latency (TL) in the EPM, shortened latency to reach the shock-free zone (SFZ), and an increase in rearing and counts by locomotion activity in the OFT. It also demonstrated improved anti-cholinesterase activity and suppressed oxidative stress, as indicated by a decrease in nitric oxide (NO) levels and an increase in superoxide dismutase (SOD) and catalase levels. Additionally, it led to reductions in brain changes observed in histopathological analysis.
CONCLUSION
Atorvastatin with magnesium L-threonate provides a better beneficial protective effect against AD than atorvastatin alone. This combination can be a first choice for patients who are already taking atorvastatin in the early stages of AD.
PubMed: 38074017
DOI: 10.7759/cureus.48400 -
Animals : An Open Access Journal From... Nov 2023A wide variety of substances have been used to anaesthetise invertebrates, but many are not anaesthetics and merely incapacitate animals rather than preventing pain. In...
A wide variety of substances have been used to anaesthetise invertebrates, but many are not anaesthetics and merely incapacitate animals rather than preventing pain. In essence, the role of an ideal general anaesthetic is to act as a muscle relaxant, an analgesic, an anaesthetic, and an amnesic. To achieve all these properties with a single substance is difficult, and various adjuvants usually need to be administered, resulting in a cocktail of drugs. In a clinical setting, the vast majority of patients are unaware of surgery being carried out and have no memory of it, so they can claim to have felt no pain, but this is much more difficult to demonstrate in invertebrates. Here, we show that 1% MgCl a muscle relaxant is a useful adjuvant for the clinical anaesthetic isoflurane on when applied alone in seawater for 10 min before the clinical anaesthetic. After this, full anaesthesia can be achieved in 5 min using 1% isoflurane insufflated into the saline still containing MgCl. Full recovery takes place rapidly in about 10 to 15 min. The depth of anaesthesia was monitored using changes in respiratory rate, chromatophore pattern, and withdrawal movements of the arms and siphon. This methodology reduces stress on the animal and minimises the quantity of anaesthetic used.
PubMed: 38003196
DOI: 10.3390/ani13223579 -
Scientific Reports Mar 2022This retrospective study aimed to explore the relationships between electrolytes and osmotic pressure homeostasis with contrast-associated acute kidney injury (CA-AKI)...
This retrospective study aimed to explore the relationships between electrolytes and osmotic pressure homeostasis with contrast-associated acute kidney injury (CA-AKI) risk in patients with percutaneous coronary intervention or coronary angiography. We totally enrolled 4386 hospitalized patients, who were categorized into five groups based on the predetermined cutoff values of electrolytes and osmotic pressure. CA-AKI was defined as an increase in serum creatine by 0.5 mg/dL (44.2 mol/L) or a 25% increase of the highest level post-operation compared to baseline. Multivariable logistic analysis was used to examine the association of CA-AKI incidence with electrolytes and osmotic pressure levels. Piecewise linear regression models and restricted cubic spline analysis were further utilized to determine the nonlinear relationship. The results showed U-shaped relationships between sodium, chloride, magnesium, and osmotic pressure levels and CA-AKI incidence. The lowest incidence was observed in the categories of 139-141.9 mmol/L, 107.0-109.9 mmol/L, 0.91-1.07 mmol/L, and 290.0-299.9 mOsm/kg, respectively. J-shaped associations were observed for potassium and phosphate levels and CA-AKI incidence, with the lowest incidence in the categories of 3.50-4.09 mmol/L and 0.96-1.28 mmol/L, respectively. A negative correlation was observed between calcium level and CA-AKI incidence, with the lowest CA-AKI risk in the category of ≥ 2.58 mmol/L. In conclusion, abnormally higher or lower sodium, chloride, magnesium, phosphate, and osmotic pressure levels on admission were associated with increased risks of CA-AKI. While for potassium and calcium, the status of hyperkalemia and hypocalcemia on admission showed more susceptibility for CA-AKI.
Topics: Acute Kidney Injury; Calcium; Contrast Media; Electrolytes; Female; Humans; Incidence; Magnesium; Magnesium Chloride; Male; Osmotic Pressure; Percutaneous Coronary Intervention; Phosphates; Potassium; Retrospective Studies; Risk Factors; Sodium
PubMed: 35304524
DOI: 10.1038/s41598-022-08597-z -
Environmental Monitoring and Assessment Dec 2020To investigate the physical, chemical, and mineralogical characteristics of peloids, which are being used traditionally and historically across Turkey, and evaluate...
To investigate the physical, chemical, and mineralogical characteristics of peloids, which are being used traditionally and historically across Turkey, and evaluate their suitability and potential for use in peloidotherapy. Five peloid samples were gathered from their places of origin, namely Gölemezli, Dalyan, Köprüköy, Gökçeada, and Dikili. Mineral analysis and physical and chemical analyses including electrical conductivity, density, cations, anions, trace elements, organic matters, and water retention capacity at 105 °C were performed. The peloids contained a combination of clay minerals (mainly montmorillonite, kaolinite, and muscovite) and non-clay minerals (mainly quartz, calcite, dolomite, and albite) except for Gölemezli peloid, which was dominated by calcite. The other minerals (i.e., chloride-serpentine, sphalerite, pyrite, magnesium calcite, cristobalite) were also found in some peloids. Gölemezli, Dalyan, and Köprüköy peloids had high total organic matters, mainly humic substances. The water retention capacity was high in Dalyan, Köprüköy, and Dikili peloids. All peloids had a pH value slightly greater than 7 (range 7.93-8.69). Dalyan, Köprüköy, and Dikili peloids had a high water retention capacity. Dalyan and Gökçeada peloids had a high electrical conductivity, 22.040 and 9.020 μS/cm, respectively. The density of peloids was ranged between 1.240 (Gölemezli) and 1.450 (Gökçeada) g/cm. Total mineralization of investigated peloids was greater than 1000 mg/L: Köprüköy, 2754.8 mg/L; Gölemezli, 3092.8 mg/L; Dikili, 4044.6 mg/L; Gökçeada, 6576.6 mg/L; and Dalyan, 11782.9 mg/L, mainly sodium, magnesium, calcium, chloride, sulfate, bicarbonate, and metasilicic acid. The levels of trace elements were low (≤ 2.0 mg/L) in all peloids. The peloids contained various amounts of clay minerals (mainly montmorillonite, kaolinite, and muscovite), non-clay minerals (mainly quartz, calcite, dolomite, and albite), organic matters (mainly humic acid), cations (mainly sodium, magnesium, and calcium), anions (mainly chloride, sulfate, and bicarbonate), and insoluble compounds (mainly metasilicic acid). The physical, chemical, and mineralogical properties of peloids suggest their suitability and potential for use in peloidotherapeutic applications.
Topics: Clay; Environmental Monitoring; Humic Substances; Minerals; Turkey
PubMed: 33263866
DOI: 10.1007/s10661-020-08777-2