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Journal of Cellular and Molecular... Feb 2024Blood vessels are essential for bone development and metabolism. Type H vessels in bone, named after their high expression of CD31 and Endomucin (Emcn), have recently... (Review)
Review
Blood vessels are essential for bone development and metabolism. Type H vessels in bone, named after their high expression of CD31 and Endomucin (Emcn), have recently been reported to locate mainly in the metaphysis, exhibit different molecular properties and couple osteogenesis and angiogenesis. A strong correlation between type H vessels and bone metabolism is now well-recognized. The crosstalk between type H vessels and osteoprogenitor cells is also involved in bone metabolism-related diseases such as osteoporosis, osteoarthritis, fracture healing and bone defects. Targeting the type H vessel formation may become a new approach for managing a variety of bone diseases. This review highlighted the roles of type H vessels in bone-related diseases and summarized the research attempts to develop targeted intervention, which will help us gain a better understanding of their potential value in clinical application.
Topics: Humans; Osteogenesis; Bone and Bones; Osteoporosis; Bone Diseases, Metabolic; Fracture Healing; Neovascularization, Physiologic
PubMed: 38353470
DOI: 10.1111/jcmm.18123 -
International Journal of Oncology Nov 2023Osteosarcoma (OS) prevailing in children and adolescents mainly occurs at the metaphysis of long bones. As it is associated with a high invasive and metastatic ability,... (Review)
Review
Osteosarcoma (OS) prevailing in children and adolescents mainly occurs at the metaphysis of long bones. As it is associated with a high invasive and metastatic ability, resistance to chemotherapy, and a low 5‑year survival rate, the diagnosis and treatment of OS post a global healthy issue. Over the past decades, RNA biology has shed new light onto the pathogenesis of OS. As a type of non‑coding RNAs, circular RNAs (circRNAs) have been found to play crucial roles in cellular activities. Recently, a large number of circRNAs have been identified in OS and some of them have been validated to be functional in OS. In the present review, abnormally expressed and different types of circRNAs in OS are summarized. Functional studies on circRNAs have revealed that circRNAs can regulate gene expression at different levels, such as gene transcription, precursor mRNA splicing, miRNA sponges and translation into proteins/peptides. Mechanistic analyses on circRNAs show that circRNAs can regulate JAK‑STAT3, NF‑κB, PI3K‑AKT, Wnt/β‑catenin signaling pathways during the occurrence and development of OS. Furthermore, the potential clinical applications of circRNAs are also emphasized. The present review focus on the current knowledge on the functions and mechanisms of circRNAs in the pathogenesis of OS, aiming to provide new insight into the OS diagnosis and treatment of OS.
Topics: Adolescent; Child; Humans; RNA, Circular; Phosphatidylinositol 3-Kinases; MicroRNAs; Osteosarcoma; Bone Neoplasms
PubMed: 37681483
DOI: 10.3892/ijo.2023.5571 -
Cureus Feb 2020Slipped capital femoral epiphysis (SCFE) is a frequent cause of nontraumatic painful hip of the adolescence. It is the result of the separation of the proximal femoral... (Review)
Review
Slipped capital femoral epiphysis (SCFE) is a frequent cause of nontraumatic painful hip of the adolescence. It is the result of the separation of the proximal femoral growth cartilage at the level of the hypertrophic cell zone. The femoral neck metaphysis rotates externally and migrates proximally relative to the femoral head epiphysis, which is stably seated in the acetabulum; early diagnosis and in situ stabilization grants the best long term results. Numerous factors affect treatment outcomes. Not all implants have the same effect on the slipped physis. Application of the traditionally used implants, such as non-threaded pins and cannulated screws, is questioned. Modern implants are available, which stabilize the slip without accelerating physis fusion. This allows femoral head and neck growth and remodeling to limit the post-slip sequellae on hip anatomy and function. Femoroacetabular impingement (FAI) complicates almost all slips. It causes progressive labral and articular cartilage damage and leads to early hip osteoarthritis and total hip replacement, approximately ten years earlier compared to the general population. Avascular necrosis of the femoral head is a dramatic complication, seen almost exclusively in unstable slips. It develops within months after the slip and leads to immediate articular joint degeneration and the need for total hip replacement. Another serious complication of SCFE is chondrolysis, which is a rapid progressive articular cartilage degeneration leading to a narrow joint space and restriction of hip motion. Implant-related complications, such as migration and loosening, may lead to the progression of the slip. Though bilateral disease is quite frequent, there is no consensus about the need for preventive surgery on the healthy contralateral hip. Diagnosis of SCFE is frequently missed or delayed, leading to slips of higher severity. Silent slippage of the capital femoral epiphysis is highly suspected as an underlying cause of cam-type FAI and early-onset hip osteoarthritis. There is controversy, whether asymptomatic implants should be removed. Novel surgical techniques, such as the modified Dunn procedure and hip arthroscopy, seem to be effective modalities for the prevention of FAI in SCFE.
PubMed: 32190446
DOI: 10.7759/cureus.6883 -
Magnetic Resonance Imaging Clinics of... Nov 2019Normal bone growth of the pediatric knee as well as normal variants of ossification result in different appearances that can be identified on imaging (radiography/MR... (Review)
Review
Normal bone growth of the pediatric knee as well as normal variants of ossification result in different appearances that can be identified on imaging (radiography/MR imaging). Familiarity with these changes is important to avoid confusing normal growth with pathology. This article illustrates the main features related to normal bone growth (growth arrest lines, physeal changes, ossification centers within the epiphysis, hematopoietic marrow within the metaphysis) and physis disappearance (« FOPE »). Variants in femur (epiphyseal irregularities, subchondral anomalies of posterior condyles, periosteal desmoid), tibia (tibial tuberosity ossification), and patella (dorsal defect, bipartite patella, lower pole fragmentation) are also described.
Topics: Bone Development; Child; Humans; Knee; Knee Joint; Magnetic Resonance Imaging; Radiography
PubMed: 31575403
DOI: 10.1016/j.mric.2019.07.013 -
Clinical Anatomy (New York, N.Y.) May 2024Division of the growing long bone into individual basic parts, that is, diaphysis, metaphysis, physes and epiphyses, has become generally accepted and used. However, the... (Review)
Review
Division of the growing long bone into individual basic parts, that is, diaphysis, metaphysis, physes and epiphyses, has become generally accepted and used. However, the origin of these terms is almost unknown. Therefore, we have analyzed the literature in order to identify their sources. The terms epiphysis and apophysis have been used since the time of Hippokrates, although with different meanings. During the time of Galen, the term apophysis was used to describe all types of bone processes, and epiphyses denoted articular ends. The term diaphysis denoting the middle cylindrical part of the long bone was used for the first time by Heister in 1717. The first to use the term metaphysis was Theodor Kocher in his books on gunshot wounds and on bone inflammation of 1895. On the basis of Kocher's study, Lexer published a radiological study of the vascular supply to bones in which he defined metaphyseal blood vessels as a separate group supplying a particular part of the long bone. The epiphyseal growth plate had no particular name from the time of its first description in 1836. During the second half of 19th century, this structure acquired different names. The term "physis" was therefore introduced in 1964 by the American radiologist Rubin in order to label the growth structure between metaphysis and epiphysis clearly. One year later, the term physis also appeared in the radiological literature, and during the following decades it spread in the orthopedic literature.
PubMed: 38778675
DOI: 10.1002/ca.24176 -
Frontiers in Endocrinology 2022Skeletal stem and progenitor cells (SSPCs) constitute a reservoir of bone-forming cells necessary for bone development, modeling and remodeling, as well as for fracture...
Skeletal stem and progenitor cells (SSPCs) constitute a reservoir of bone-forming cells necessary for bone development, modeling and remodeling, as well as for fracture healing. Recent advances in tools to identify and isolate SSPCs have revealed that cells with multipotent properties are present not only in neonatal bone, but also in adult bone marrow and periosteum. The long bone metaphysis and endosteum have been proposed as an additional SSPC niche, although approaches to study their cellular and molecular characteristics are still limited. Here, we describe a comprehensive procedure to isolate and culture SSPCs derived from the metaphysis and endosteum of young-adult mice. Based on flow cytometry analysis of known SSPC markers, we found the presence of putative multipotent SSPCs, similar to neonatal bone tissue. , metaphyseal/endosteal SSPCs possess self-renewing capacity, and their multipotency is underscored by the ability to differentiate into the osteogenic and adipogenic lineage, while chondrogenic potential is limited. Expansion of metaphyseal/endosteal SSPCs under low oxygen conditions increases their proliferation capacity, while progenitor properties are maintained, likely reflecting their hypoxic niche . Collectively, we propose a validated isolation and culture protocol to study metaphyseal/endosteal SSPC biology .
Topics: Animals; Bone and Bones; Cell Differentiation; Fracture Healing; Mice; Osteogenesis; Stem Cells
PubMed: 35979436
DOI: 10.3389/fendo.2022.930358 -
The Knee Oct 2023Excessive posterior tibial slope (PTS) is an independent risk factor for anterior cruciate ligament reconstruction (ACLR) failure, but it remains unclear how PTS relates...
BACKGROUND
Excessive posterior tibial slope (PTS) is an independent risk factor for anterior cruciate ligament reconstruction (ACLR) failure, but it remains unclear how PTS relates to other proximal tibial morphologic parameters. The purpose of this study was to analyse sagittal tibial metaphysis morphology, and to calculate the correlation coefficients of PTS with anatomical features.
METHODS
The authors retrospectively reviewed lateral radiographs of 350 patients that were scheduled to receive primary ACLR to digitize 15 landmarks on the patella, femur, fibula, and tibia, and measure PTS, patellar height, as well as metaphysis height and inclination. Pearson correlation coefficients (r) were computed to assess the linear relationship of PTS with other parameters.
RESULTS
The PTS was 9.8 ± 3.1° (mid-shaft axis), anterior metaphyseal height and inclination was 30.9 ± 4.6 mm and 33.9 ± 7.2°, and posterior metaphyseal height and inclination was 16.1 ± 4.0 mm and 22.0 ± 5.8°. PTS had a low correlation with anterior (r, 0.225) and posterior metaphyseal heights (r, -0.183). PTS had moderate correlations with anterior (r, 0.385) and posterior metaphysis inclination (r, 0.417).
CONCLUSION
PTS has a low correlation with anterior metaphyseal height, but a moderate correlation with anterior and posterior metaphyseal inclination. The moderate correlation between PTS and metaphysis inclination sheds light on the origin of the deformity, and knees with higher PTS are therefore likely to have metaphyses with greater posterior inclinations. The clinical relevance of these findings is that tibial deflexion osteotomy techniques should attempt to address the underlying deformity of excessive PTS by adjusting metaphyseal inclination rather than making diaphyseal resections.
Topics: Humans; Tibia; Knee Joint; Retrospective Studies; Femur; Radiography; Anterior Cruciate Ligament Injuries
PubMed: 37717277
DOI: 10.1016/j.knee.2023.08.007 -
Journal of Orthopaedic Case Reports Jan 2023Chondromyxoid fibroma (CMF) is an uncommon bone tumor, accounting for <1% of all bone tumors. It generally affects the metaphysis of the long bone of the lower limb and...
INTRODUCTION
Chondromyxoid fibroma (CMF) is an uncommon bone tumor, accounting for <1% of all bone tumors. It generally affects the metaphysis of the long bone of the lower limb and seldom involves the small bones, but the involvement of the flat bones, like ribs, is infrequent.
CASE REPORT
A teenage girl complained of pain on the right side of the chest for the past 6 months, dull aching, and persistent without any diurnal variation. The chest X-ray examination showed a nodular, hyperdense lesion on the lateral chest wall near the 5-7th rib on the right side. A computed tomography scan confirmed the lesion from the sixth rib with ground-glass matrix and cortical thinning with a smooth margin, without any soft-tissue involvement. An enblock excision of the lesion was done. Histopathological studies showed a well-circumscribed tumor with reactive bone formation at the periphery with lobules of chondromyxoid tissue separated by spindle cells and stellate-shaped cells suggestive of CMF. At 1-year follow-up, she is asymptomatic without any recurrence.
CONCLUSION
CMFs are rare benign tumors that require histopathological study to differentiate from other benign lesions of the bone. In flat tubular bones like the ribs, enblock resection is the mainstay of treatment.
PubMed: 37143567
DOI: 10.13107/jocr.2023.v13.i01.3528 -
Frontiers in Bioengineering and... 2021The growth plate (GP) is a cartilaginous region situated between the epiphysis and metaphysis at the end of the immature long bone, which is susceptible to mechanical... (Review)
Review
The growth plate (GP) is a cartilaginous region situated between the epiphysis and metaphysis at the end of the immature long bone, which is susceptible to mechanical damage because of its vulnerable structure. Due to the limited regeneration ability of the GP, current clinical treatment strategies (e.g., bone bridge resection and fat engraftment) always result in bone bridge formation, which will cause length discrepancy and angular deformity, thus making satisfactory outcomes difficult to achieve. The introduction of cartilage repair theory and cartilage tissue engineering technology may encourage novel therapeutic approaches for GP repair using tissue engineered GPs, including biocompatible scaffolds incorporated with appropriate seed cells and growth factors. In this review, we summarize the physiological structure of GPs, the pathological process, and repair phases of GP injuries, placing greater emphasis on advanced tissue engineering strategies for GP repair. Furthermore, we also propose that three-dimensional printing technology will play a significant role in this field in the future given its advantage of bionic replication of complex structures. We predict that tissue engineering strategies will offer a significant alternative to the management of GP injuries.
PubMed: 34150725
DOI: 10.3389/fbioe.2021.654087 -
IScience Sep 2023Type H vessels couple angiogenesis with osteogenesis, while sympathetic cues regulate vascular and skeletal function. The crosstalk between sympathetic nerves and type H...
Type H vessels couple angiogenesis with osteogenesis, while sympathetic cues regulate vascular and skeletal function. The crosstalk between sympathetic nerves and type H vessels in bone remains unclear. Here, we first identify close spatial connections between sympathetic nerves and type H vessels in bone, particularly in metaphysis. Sympathoexcitation, mimicked by isoproterenol (ISO) injection, reduces type H vessels and bone mass. Conversely, beta-2-adrenergic receptor (ADRB2) deficiency maintains type H vessels and bone mass in the physiological condition. experiments reveal indirect sympathetic modulation of angiogenesis via paracrine effects of mesenchymal stem cells (MSCs), which alter the transcription of multiple angiogenic genes in endothelial cells (ECs). Furthermore, Notch signaling in ECs underlies sympathoexcitation-regulated type H vessel formation, impacting osteogenesis and bone mass. Finally, propranolol (PRO) inhibits beta-adrenergic activity and protects type H vessels and bone mass against estrogen deficiency. These findings unravel the specialized neurovascular coupling in bone homeostasis and regeneration.
PubMed: 37680481
DOI: 10.1016/j.isci.2023.107455