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Journal of Biomedical Optics Jan 2021Surgical microscopes provide adjustable magnification, bright illumination, and clear visualization of the surgical field and have been increasingly used in operating... (Review)
Review
SIGNIFICANCE
Surgical microscopes provide adjustable magnification, bright illumination, and clear visualization of the surgical field and have been increasingly used in operating rooms. State-of-the-art surgical microscopes are integrated with various imaging modalities, such as optical coherence tomography (OCT), fluorescence imaging, and augmented reality (AR) for image-guided surgery.
AIM
This comprehensive review is based on the literature of over 500 papers that cover the technology development and applications of surgical microscopy over the past century. The aim of this review is threefold: (i) providing a comprehensive technical overview of surgical microscopes, (ii) providing critical references for microscope selection and system development, and (iii) providing an overview of various medical applications.
APPROACH
More than 500 references were collected and reviewed. A timeline of important milestones during the evolution of surgical microscope is provided in this study. An in-depth technical overview of the optical system, mechanical system, illumination, visualization, and integration with advanced imaging modalities is provided. Various medical applications of surgical microscopes in neurosurgery and spine surgery, ophthalmic surgery, ear-nose-throat (ENT) surgery, endodontics, and plastic and reconstructive surgery are described.
RESULTS
Surgical microscopy has been significantly advanced in the technical aspects of high-end optics, bright and shadow-free illumination, stable and flexible mechanical design, and versatile visualization. New imaging modalities, such as hyperspectral imaging, OCT, fluorescence imaging, photoacoustic microscopy, and laser speckle contrast imaging, are being integrated with surgical microscopes. Advanced visualization and AR are being added to surgical microscopes as new features that are changing clinical practices in the operating room.
CONCLUSIONS
The combination of new imaging technologies and surgical microscopy will enable surgeons to perform challenging procedures and improve surgical outcomes. With advanced visualization and improved ergonomics, the surgical microscope has become a powerful tool in neurosurgery, spinal, ENT, ophthalmic, plastic and reconstructive surgeries.
Topics: Industrial Development; Microscopy; Neurosurgical Procedures; Surgery, Computer-Assisted; Tomography, Optical Coherence
PubMed: 33398948
DOI: 10.1117/1.JBO.26.1.010901 -
Journal of Biophotonics Jun 2020Having the least lenses, the significant feature of the singlet imaging system, helps the development of the portable and cost-effective microscopes. A novel method of...
Having the least lenses, the significant feature of the singlet imaging system, helps the development of the portable and cost-effective microscopes. A novel method of monochromatic/color singlet microscopy, which is combined with only one aspheric lens and deep learning computational imaging technology, is proposed in this article. The designed singlet aspheric lens is an approximate linear signal system, which means modulation-transfer-function curves on all field-of-views (5 mm diagonally) are almost coincident with each other. The purpose of the designed linear signal system is to further improve the resolution of our microscope by using deep learning algorithm. As a proof of concept, we designed a singlet microscopy based on our method, which weighs only 400 g. The experimental data and results of the sample USAF-1951 target and bio-sample (the Equisetum-arvense Strobile L.S), prove that the performance of the proposed singlet microscope is competitive to a commercial microscope with the 4X/NA0.1 objective lens. We believe that our idea and method would guide to design more cost-effective and powerful singlet imaging system.
Topics: Algorithms; Deep Learning; Equipment Design; Lenses; Microscopy
PubMed: 32125774
DOI: 10.1002/jbio.202000013 -
Endeavour Sep 2019This article draws attention to literary works which have been influenced by microscopy, or in which microscopy has played a significant role. The work of writers...
This article draws attention to literary works which have been influenced by microscopy, or in which microscopy has played a significant role. The work of writers including Jonathan Swift, Voltaire, George Eliot, H. G. Wells and D. H. Lawrence is discussed. In many cases these authors had direct experience of operating microscopes and viewing the wonders of the microscopic world. However, with the increasing separation of the two cultures, recent examples of microscopy in literature are rare.
PubMed: 31668793
DOI: 10.1016/j.endeavour.2019.100695 -
Medicine and Pharmacy Reports Jan 2021Magnification devices improve direct and indirect vision and precision being significantly higher in microscope use compared to the loupes. Dental loupes are the most... (Review)
Review
Magnification devices improve direct and indirect vision and precision being significantly higher in microscope use compared to the loupes. Dental loupes are the most commonly used devices for magnification, due to the more affordable prices and the ease of use without major changes in the working protocol and ergonomics. Loupes primary benefits reported refers to ergonomics and posture, restoration evaluation/detection and overall treatment quality. There are some disadvantages that limit the use of loupes among dentists: lack of fixed position (fine movements of the dentist's head disturb the image of the magnified operating field); the need to change the loupes to achieve different magnification. On the contrary, the use of a dental microscope require minimum adjustment and effort so as to reduce postural deviation while working. It has been more than 30 years since dental practitioners raised the issue of using dental operating microscopes (DOM) in restorative dentistry, identifying the benefits of magnification in diagnosis and treatment steps. The growth is evident in recent years, maybe because of the familiarity of dentists with dental microscopes which have already become implicit endowment for endodontic specialists. Fiber optic lighting is the key factor that complements the magnification offered by the microscope so that treatments can now be performed under increased safety conditions and in conditions of significantly higher quality than in the past. Since the beginning of the 2000s the principles of minimally invasive dentistry have been widely promoted in dentistry. These principles are major impetus for the use of microscope by dentists. Working with magnification leads dentists to be more conservative with dental tissues.
PubMed: 33629044
DOI: 10.15386/mpr-1662 -
Clinics in Dermatology 2021Dermatology is one of the few visual specialties; much can be seen with the naked eye, yet many tools and devices can facilitate the diagnosis of cutaneous maladies....
Dermatology is one of the few visual specialties; much can be seen with the naked eye, yet many tools and devices can facilitate the diagnosis of cutaneous maladies. There has been significant progress in diagnostic techniques in recent decades with the advent of artificial intelligence and teledermatology. Such advancements reflect principles of long-standing aids, whereas others embody novel techniques and concepts. These bedside tools range from simple magnifiers and binocular loupes to reflectance confocal microscopes and ultrasounds. The cornerstone diagnostic techniques, including the potassium hydroxide examination and the Tzanck smear, remain highly useful. Although visual examination often permits an accurate diagnosis of a skin disease, the clinician is now even further equipped. Just as the microscope did 4 centuries ago, newer advances will continue to revolutionize the practice of medicine.
Topics: Artificial Intelligence; Dermatology; Humans; Skin; Skin Diseases; Staining and Labeling
PubMed: 34809761
DOI: 10.1016/j.clindermatol.2021.03.002 -
Inflammation and Regeneration Feb 2023Inflammation can contribute to the development and progression of cancer. The inflammatory responses in the tumor microenvironment are shaped by complex sequences of... (Review)
Review
Inflammation can contribute to the development and progression of cancer. The inflammatory responses in the tumor microenvironment are shaped by complex sequences of dynamic intercellular cross-talks among diverse types of cells, and recapitulation of these dynamic events in vitro has yet to be achieved. Today, intravital microscopy with two-photon excitation microscopes (2P-IVM) is the mainstay technique for observing intercellular cross-talks in situ, unraveling cellular and molecular mechanisms in the context of their spatiotemporal dynamics. In this review, we summarize the current state of 2P-IVM with fluorescent indicators of signal transduction to reveal the cross-talks between cancer cells and surrounding cells including both immune and non-immune cells. We also discuss the potential application of red-shifted indicators along with optogenetic tools to 2P-IVM. In an era of single-cell transcriptomics and data-driven research, 2P-IVM will remain a key advantage in delivering the missing spatiotemporal context in the field of cancer research.
PubMed: 36750856
DOI: 10.1186/s41232-023-00261-x -
Lab on a Chip Nov 2021We report the Muscope, a miniature lensless holographic microscope suitable for on-chip integration. The prototype of the Muscope measured approximately only 7 mm × 4...
We report the Muscope, a miniature lensless holographic microscope suitable for on-chip integration. The prototype of the Muscope measured approximately only 7 mm × 4 mm × 4 mm, and was capable of offering a sub-micron half-pitch resolution. We have used, for the first time, a microLED display as the light source in a microscope. The individual pixels of a microLED display chip are used as programmable, microscopic and intense LEDs which can be spatially moved in a two-dimensional plane with a 5 μm pitch. This unique feature set of the display was used to implement computational super-resolution and wide-field imaging without any extra hardware, unlike many other lensless microscopes. We also report a new method to evaluate the magnification in our setting. The Muscope surpasses the existing lensless microscopes in compactness, scalability for production, automated operation and system integration. It provides exciting opportunities for a new class of devices with in-built optical imaging and monitoring and/or sensing capabilities.
Topics: Holography; Microscopy; Optical Imaging
PubMed: 34723299
DOI: 10.1039/d1lc00792k -
Annals of Anatomy = Anatomischer... Jun 2024In this manuscript, the structure of the human choroid is reviewed with emphasis of the macro- and microscopic anatomy including Bruch's membrane, choriocapillaris,... (Review)
Review
In this manuscript, the structure of the human choroid is reviewed with emphasis of the macro- and microscopic anatomy including Bruch's membrane, choriocapillaris, Sattler's and Haller's layer, and the suprachoroid. We here discuss the development of the choroid, as well as the question of choroidal lymphatics, and further the neuronal control of this tissue, as well as the pathologic angiogenesis. Wherever possible, functional aspects of the various structures are included and reviewed.
Topics: Humans; Choroid; Bruch Membrane
PubMed: 38432349
DOI: 10.1016/j.aanat.2024.152239 -
Chemical Reviews Jul 2023Catalysts are the primary facilitator in many dynamic processes. Therefore, a thorough understanding of these processes has vast implications for a myriad of energy... (Review)
Review
Catalysts are the primary facilitator in many dynamic processes. Therefore, a thorough understanding of these processes has vast implications for a myriad of energy systems. The scanning/transmission electron microscope (S/TEM) is a powerful tool not only for atomic-scale characterization but also catalytic experimentation. Techniques such as liquid and gas phase electron microscopy allow the observation of catalysts in an environment conducive to catalytic reactions. Correlated algorithms can greatly improve microscopy data processing and expand multidimensional data handling. Furthermore, new techniques including 4D-STEM, atomic electron tomography, cryogenic electron microscopy, and monochromated electron energy loss spectroscopy (EELS) push the boundaries of our comprehension of catalyst behavior. In this review, we discuss the existing and emergent techniques for observing catalysts using S/TEM. Challenges and opportunities highlighted aim to inspire and accelerate the use of electron microscopy to further investigate the complex interplay of catalytic systems.
PubMed: 37327473
DOI: 10.1021/acs.chemrev.2c00880 -
Journal of Cell Science Oct 2021Custom-built microscopes often require control of multiple hardware devices and precise hardware coordination. It is also desirable to have a solution that is scalable...
Custom-built microscopes often require control of multiple hardware devices and precise hardware coordination. It is also desirable to have a solution that is scalable to complex systems and that is translatable between components from different manufacturers. Here we report Python-Microscope, a free and open-source Python library for high-performance control of arbitrarily complex and scalable custom microscope systems. Python-Microscope offers simple to use Python-based tools, abstracting differences between physical devices by providing a defined interface for different device types. Concrete implementations are provided for a range of specific hardware, and a framework exists for further expansion. Python-Microscope supports the distribution of devices over multiple computers while maintaining synchronisation via highly precise hardware triggers. We discuss the architectural features of Python-Microscope that overcome the performance problems often raised against Python and demonstrate the different use cases that drove its design: integration with user-facing projects, namely the Microscope-Cockpit project; control of complex microscopes at high speed while using the Python programming language; and use as a microscope simulation tool for software development.
Topics: Computer Simulation; Gene Library; Software
PubMed: 34448002
DOI: 10.1242/jcs.258955